Planarian EST Database


Dr_sW_020_A06

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_020_A06
         (844 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9V0D5|MDH_PYRAB  Malate dehydrogenase                         213   7e-55
sp|O59028|MDH_PYRHO  Malate dehydrogenase                         208   2e-53
sp|P16142|MDH_METFE  Malate/L-sulfolactate dehydrogenase ((R...   181   3e-45
sp|Q58820|COMC_METJA  L-sulfolactate dehydrogenase ((R)-sulf...   174   3e-43
sp|O27273|COMC_METTH  L-sulfolactate dehydrogenase ((R)-sulf...   163   5e-40
sp|O34736|YJMC_BACSU  Hypothetical oxidoreductase yjmC            160   4e-39
sp|P58408|ALLD_ECO57  Ureidoglycolate dehydrogenase               140   6e-33
sp|P77555|ALLD_ECOLI  Ureidoglycolate dehydrogenase               138   2e-32
sp|P58409|YBIC_ECO57  Hypothetical oxidoreductase ybiC            119   1e-26
sp|P30178|YBIC_ECOLI  Hypothetical oxidoreductase ybiC            117   3e-26
>sp|Q9V0D5|MDH_PYRAB Malate dehydrogenase
          Length = 362

 Score =  213 bits (541), Expect = 7e-55
 Identities = 119/270 (44%), Positives = 171/270 (63%), Gaps = 3/270 (1%)
 Frame = +2

Query: 29  FKKCMSTESVIIAPT-EVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLE 205
           F+K    E+ +  P  E+  F ++ +  + T  E +K +AD L+ AD  G  SHG+ RL+
Sbjct: 2   FEKGYVDENYVRIPKDELFSFVVRVLTKLGTPEEDAKIVADNLIMADLRGIESHGVQRLK 61

Query: 206 MYVDDIKANITSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVA 385
            YVD I +   +      II E  + A +DG    G VVG  +M +AI KA + G+G VA
Sbjct: 62  RYVDGILSGGINLHPRIKIIREGPSYALLDGDEGFGQVVGYKAMKLAIEKARKTGIGIVA 121

Query: 386 AKGSNHYGIAGWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESD 565
            + SNHYGIAG+Y+  A ++G++G+S TN+ PL  PT   +  +GTNP+ALAAP   +  
Sbjct: 122 VRNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGVERILGTNPIALAAP--TKGK 179

Query: 566 NFLLDMATTAVALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGG-LLPLGGI-EI 739
            FLLDMAT+ V +GK+E+ RRK E IPEGWA ++ G++T+  +E   GG LLPLGG  E+
Sbjct: 180 PFLLDMATSVVPIGKLEVYRRKGEEIPEGWAINSKGEITRSVEEVFNGGSLLPLGGFGEL 239

Query: 740 TGGYKGYGLSMLVEIFCGILSGSLYGPQIR 829
            GG+KGYGLS++V+I  GILSG  +   ++
Sbjct: 240 LGGHKGYGLSLMVDILSGILSGGTWSKHVK 269
>sp|O59028|MDH_PYRHO Malate dehydrogenase
          Length = 360

 Score =  208 bits (529), Expect = 2e-53
 Identities = 118/270 (43%), Positives = 169/270 (62%), Gaps = 3/270 (1%)
 Frame = +2

Query: 29  FKKCMSTESVIIAPTE-VREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLE 205
           F+K    E+ I  P + +  F ++ +  +    E +K +AD L+ AD  G  SHG+ RL+
Sbjct: 2   FEKGYVDENYIRVPKDRLFSFIVRVLTKLGVPEEDAKIVADNLVMADLRGVESHGVQRLK 61

Query: 206 MYVDDIKANITSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVA 385
            YVD I +   +   +  +I E  + A +DG   LG VVG  SM +AI KA   G+G V 
Sbjct: 62  RYVDGIISGGVNLHPKIRVIREGPSYALIDGDEGLGQVVGYRSMKLAIKKAKDTGIGIVI 121

Query: 386 AKGSNHYGIAGWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESD 565
           A+ SNHYGIAG+Y+  A ++G++G+S TN+ PL  PT   +  +GTNP+ALAAP + +  
Sbjct: 122 ARNSNHYGIAGYYALMAAEEGMIGISMTNSRPLVAPTGGIERILGTNPIALAAPTKDKP- 180

Query: 566 NFLLDMATTAVALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGG-LLPLGGI-EI 739
            FLLDMAT+ V +GK+E+ RRK + IPEGWA +  G +T   +E   GG LLPLGG  E+
Sbjct: 181 -FLLDMATSVVPIGKLEVYRRKGKDIPEGWAINREGNITTKVEEVFNGGALLPLGGFGEL 239

Query: 740 TGGYKGYGLSMLVEIFCGILSGSLYGPQIR 829
            GG+KGYGLS++V+I  GILSG  +   ++
Sbjct: 240 LGGHKGYGLSLMVDILSGILSGGTWSKYVK 269
>sp|P16142|MDH_METFE Malate/L-sulfolactate dehydrogenase ((R)-2-hydroxyacid
           dehydrogenase)
          Length = 339

 Score =  181 bits (458), Expect = 3e-45
 Identities = 102/258 (39%), Positives = 141/258 (54%)
 Frame = +2

Query: 56  VIIAPTEVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANI 235
           +II+P E R   IK +N++    EH+K  ADV++ AD  G  SHG+ R   YV+ IK   
Sbjct: 1   MIISPEEERSLIIKILNALGVSEEHAKITADVIVDADLKGFTSHGIGRFPQYVEGIKLGT 60

Query: 236 TSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIA 415
              S    I  E  + A ++G + LG VV    M +AI KA   GVG V    SNH+GIA
Sbjct: 61  IKTSGNIEIEKETDSVALINGNHLLGQVVAYKGMKLAIEKAKNTGVGIVGIHDSNHFGIA 120

Query: 416 GWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTA 595
           G+YS  A    ++G++ TNT P   P   K   +GTNP+A++ P  +      +DM+T A
Sbjct: 121 GYYSDMAMKNDMIGITMTNTEPAVAPLGGKIPVLGTNPIAISIPSNEY--YVAVDMSTAA 178

Query: 596 VALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSML 775
           VA GK+  A RK E IPEG A D NG  T   +E   G +LP       GG+KGY L  +
Sbjct: 179 VARGKLLEAARKNEKIPEGIAVDKNGNPTTDPNEALNGSILPF------GGHKGYALCFM 232

Query: 776 VEIFCGILSGSLYGPQIR 829
           +EI  G L  + +G +++
Sbjct: 233 IEILAGPLVKAEFGSKVK 250
>sp|Q58820|COMC_METJA L-sulfolactate dehydrogenase ((R)-sulfolactate dehydrogenase)
           ((R)-2-hydroxyacid dehydrogenase)
          Length = 344

 Score =  174 bits (441), Expect = 3e-43
 Identities = 101/258 (39%), Positives = 147/258 (56%)
 Frame = +2

Query: 56  VIIAPTEVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANI 235
           +I+ P   ++  I  +       E +K  ADV + AD  G  SHG+ R   Y+  +K   
Sbjct: 1   MILKPENEKKLIIDVLKKFGVPEEDAKITADVFVDADLKGFTSHGIGRFPQYITALKLGN 60

Query: 236 TSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIA 415
            +   +  I+ E  ATA +DG   LG VVG  +M++AI KA  VGVG VA + +NH+GIA
Sbjct: 61  INPKPDIKIVKESPATAVIDGDLGLGQVVGKKAMELAIKKAKNVGVGVVATRNANHFGIA 120

Query: 416 GWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTA 595
           G+YS+ A ++ ++G++ TNT P   P   K+  +GTNP+A+A  G K    F LDMAT +
Sbjct: 121 GYYSELAMNQDMIGITITNTEPAMAPFGGKEKILGTNPIAIAFKGNKY--KFSLDMATAS 178

Query: 596 VALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSML 775
           +A GKI  A RKK  IPEG A D +GK T    +  +G +LP       GG KGYGL++ 
Sbjct: 179 IARGKILEALRKKIKIPEGCAVDKDGKPTTDPAKALEGCILPF------GGPKGYGLALA 232

Query: 776 VEIFCGILSGSLYGPQIR 829
           +E+   I  G+  G +++
Sbjct: 233 IEMLSAI-GGAEVGTKVK 249
>sp|O27273|COMC_METTH L-sulfolactate dehydrogenase ((R)-sulfolactate dehydrogenase)
          Length = 341

 Score =  163 bits (413), Expect = 5e-40
 Identities = 94/255 (36%), Positives = 136/255 (53%)
 Frame = +2

Query: 62  IAPTEVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITS 241
           I+P E  +   + + ++    E S  +ADV L AD  G  SHG+ R   YVD ++     
Sbjct: 3   ISPEEEVKIIKEILTAMNVPEESSDIVADVTLDADLKGFSSHGIGRFPQYVDGLRHGTIR 62

Query: 242 KSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGW 421
              +  I  E  +TA ++G +  G VV   +M++AI KA   GVG V    SNH+G+AG+
Sbjct: 63  ADGDITIERETESTALINGNHIFGHVVAYRAMELAIEKARNTGVGLVGVHDSNHFGVAGY 122

Query: 422 YSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVA 601
           YS  A    ++G+   NT P   P   ++  +GTNP+A+  P  +   +  +DMAT+A A
Sbjct: 123 YSDMAVMNDMIGVVIANTEPAVAPIGGRKPILGTNPVAIGIPSNRYYVS--VDMATSASA 180

Query: 602 LGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVE 781
            GK+  A RK E IPE  A DA GK T   +   KG +LP       GG+KGY LS ++E
Sbjct: 181 RGKLLEAARKGESIPENVALDAEGKPTTDPEMALKGSILPF------GGHKGYALSFMIE 234

Query: 782 IFCGILSGSLYGPQI 826
           I  G L G+ +G  +
Sbjct: 235 ILAGPLVGAAFGTAV 249
>sp|O34736|YJMC_BACSU Hypothetical oxidoreductase yjmC
          Length = 337

 Score =  160 bits (405), Expect = 4e-39
 Identities = 91/261 (34%), Positives = 142/261 (54%)
 Frame = +2

Query: 50  ESVIIAPTEVREFCIKSMNSVQTKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKA 229
           +++ IA  E +E   + ++        ++ +ADVL+ AD    +SHG+   E YV+ + A
Sbjct: 2   KTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLLA 61

Query: 230 NITSKSNEPCIISEKGATAFVDGMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYG 409
              +   +P        T  +DG +  G V  + +MD AI+ A + GVG V A  S+H G
Sbjct: 62  GGINPGAQPVFKETGPVTGVLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHCG 121

Query: 410 IAGWYSQRACDKGLLGMSFTNTSPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMAT 589
              ++ Q+A D+ L+GM+ T+T  +  P   +   +GTNP+A   P  K    F+LDMAT
Sbjct: 122 ALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTNPIAYGVPA-KHKKPFILDMAT 180

Query: 590 TAVALGKIEMARRKKEPIPEGWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLS 769
           + VA GKI  AR + + IPEGW  D NG+     D+        +  +   GG KGYGLS
Sbjct: 181 SKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDK--------VVSLSTFGGPKGYGLS 232

Query: 770 MLVEIFCGILSGSLYGPQIRK 832
           ++V++F G+L+G+ +GP I K
Sbjct: 233 IVVDVFSGLLAGAAFGPHIAK 253
>sp|P58408|ALLD_ECO57 Ureidoglycolate dehydrogenase
          Length = 349

 Score =  140 bits (352), Expect = 6e-33
 Identities = 86/236 (36%), Positives = 120/236 (50%)
 Frame = +2

Query: 119 KHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITSKSNEPCIISEKGATAFVDG 298
           K EH+  +A+VL+ AD  G +SHG  R+E Y + I    T++  E  +      +A +  
Sbjct: 22  KREHAATVAEVLVYADARGIHSHGAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHA 81

Query: 299 MNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGWYSQRACDKGLLGMSFTNTS 478
            N  G V     M+ AI  A Q GV  V      H G   ++ Q+A   GL+G+S   + 
Sbjct: 82  DNAAGQVAAKMGMEHAIKTAQQNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSD 141

Query: 479 PLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVALGKIEMARRKKEPIPEGWA 658
           P+  P    ++  GTNPLA AAPGE + +    DMATT  A GK+  AR +   IP+ WA
Sbjct: 142 PMVVPFGGAEIYYGTNPLAFAAPGEGD-EILTFDMATTVQAWGKVLDARSRNMSIPDTWA 200

Query: 659 ADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVEIFCGILSGSLYGPQI 826
            D NG  T   D  A   LLP        G KGYGL M++++  G+L G  +G Q+
Sbjct: 201 VDKNGAPT--TDPFAVHALLP------AAGPKGYGLMMMIDVLSGVLLGLPFGRQV 248
>sp|P77555|ALLD_ECOLI Ureidoglycolate dehydrogenase
          Length = 349

 Score =  138 bits (347), Expect = 2e-32
 Identities = 85/236 (36%), Positives = 119/236 (50%)
 Frame = +2

Query: 119 KHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITSKSNEPCIISEKGATAFVDG 298
           K EH+  +A+VL+ AD  G +SHG  R+E Y + I    T++  E  +      +A +  
Sbjct: 22  KREHAATVAEVLVYADARGIHSHGAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHA 81

Query: 299 MNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGWYSQRACDKGLLGMSFTNTS 478
            N  G V     M+ AI  A Q GV  V      H G   ++ Q+A   G +G+S   + 
Sbjct: 82  DNAAGQVAAKMGMEHAIKTAQQNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSD 141

Query: 479 PLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVALGKIEMARRKKEPIPEGWA 658
           P+  P    ++  GTNPLA AAPGE + +    DMATT  A GK+  AR +   IP+ WA
Sbjct: 142 PMVVPFGGAEIYYGTNPLAFAAPGEGD-EILTFDMATTVQAWGKVLDARSRNMSIPDTWA 200

Query: 659 ADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVEIFCGILSGSLYGPQI 826
            D NG  T   D  A   LLP        G KGYGL M++++  G+L G  +G Q+
Sbjct: 201 VDKNGVPT--TDPFAVHALLP------AAGPKGYGLMMMIDVLSGVLLGLPFGRQV 248
>sp|P58409|YBIC_ECO57 Hypothetical oxidoreductase ybiC
          Length = 361

 Score =  119 bits (298), Expect = 1e-26
 Identities = 75/232 (32%), Positives = 114/232 (49%), Gaps = 2/232 (0%)
 Frame = +2

Query: 116 TKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITSKSNEPCIISEKGATAFVD 295
           ++ + +K +AD L+AA+ +GH SHG+  +  YV          ++   I+ E GA   +D
Sbjct: 25  SEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLD 84

Query: 296 GMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGWYSQRACDKGLLGMSFTNT 475
           G    G V  + +M + I KA Q G+  VA   S+H G  G+++++    G + + F + 
Sbjct: 85  GDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSV 144

Query: 476 --SPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVALGKIEMARRKKEPIPE 649
              P+  P   +    GTNP  +  P  K+    LLD AT+A+A GK  +A  K  P+P 
Sbjct: 145 VGIPMVAPFHGRDSRFGTNPFCVVFP-RKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPP 203

Query: 650 GWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVEIFCGILSG 805
           G   D NG  T      A     PLG +     +KGY L+ + EI  G LSG
Sbjct: 204 GCLIDVNGVPT---TNPAVMQESPLGSLLTFAEHKGYALAAMCEILGGALSG 252
>sp|P30178|YBIC_ECOLI Hypothetical oxidoreductase ybiC
          Length = 361

 Score =  117 bits (294), Expect = 3e-26
 Identities = 74/232 (31%), Positives = 114/232 (49%), Gaps = 2/232 (0%)
 Frame = +2

Query: 116 TKHEHSKALADVLLAADYSGHYSHGLNRLEMYVDDIKANITSKSNEPCIISEKGATAFVD 295
           ++ + +K +AD L+AA+ +GH SHG+  +  YV          ++    + E GA   +D
Sbjct: 25  SEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLD 84

Query: 296 GMNCLGPVVGNFSMDIAINKALQVGVGWVAAKGSNHYGIAGWYSQRACDKGLLGMSFTNT 475
           G    G V  + +M + I KA Q G+  VA   S+H G  G+++++    G + + F + 
Sbjct: 85  GDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSV 144

Query: 476 --SPLAFPTRSKQVAVGTNPLALAAPGEKESDNFLLDMATTAVALGKIEMARRKKEPIPE 649
              P+  P   +    GTNP  +  P  K++   LLD AT+A+A GK  +A  K  P+P 
Sbjct: 145 VGIPMVAPFHGRDSRFGTNPFCVVFP-RKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPP 203

Query: 650 GWAADANGKVTKIADEGAKGGLLPLGGIEITGGYKGYGLSMLVEIFCGILSG 805
           G   D NG  T      A     PLG +     +KGY L+ + EI  G LSG
Sbjct: 204 GCLIDVNGVPT---TNPAVMQESPLGSLLTFAEHKGYALAAMCEILGGALSG 252
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,248,366
Number of Sequences: 369166
Number of extensions: 2076112
Number of successful extensions: 5931
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5556
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5891
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)