Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_020_A05
(629 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q07192|MP2K2_XENLA Dual specificity mitogen-activated pr... 147 2e-35
sp|P47809|MP2K4_MOUSE Dual specificity mitogen-activated pr... 147 3e-35
sp|P45985|MP2K4_HUMAN Dual specificity mitogen-activated pr... 147 3e-35
sp|O09110|MP2K3_MOUSE Dual specificity mitogen-activated pr... 146 5e-35
sp|P46734|MP2K3_HUMAN Dual specificity mitogen-activated pr... 144 1e-34
sp|P70236|MP2K6_MOUSE Dual specificity mitogen-activated pr... 143 3e-34
sp|P52564|MP2K6_HUMAN Dual specificity mitogen-activated pr... 143 4e-34
sp|Q20347|YR62_CAEEL Putative serine/threonine-protein kina... 125 9e-29
sp|P08018|PBS2_YEAST Polymyxin B resistance protein kinase 123 3e-28
sp|P06784|STE7_YEAST Serine/threonine-protein kinase STE7 121 2e-27
>sp|Q07192|MP2K2_XENLA Dual specificity mitogen-activated protein kinase kinase 2 (MAP
kinase kinase 2) (MAPKK 2) (MAPK-ERK kinase 2)
Length = 446
Score = 147 bits (371), Expect = 2e-35
Identities = 76/161 (47%), Positives = 106/161 (65%), Gaps = 3/161 (1%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRSDVW 178
PSN L+D G +K+CDFGISG L++S+AKT + G +PY+APERI+P S GY +RSDVW
Sbjct: 279 PSNILLDTNGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVW 338
Query: 179 SLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPAL--PADSVYSDTVRNFISQCLVK 352
SLG+++ ELA +FPY +WNS+FDQL VV D PP L + +S + +F++QCL K
Sbjct: 339 SLGITLYELATGRFPYPKWNSVFDQLTQVVKGD-PPQLSNSEEREFSPSFTSFVNQCLTK 397
Query: 353 KDSDRASYETLIKHGMLTPFKDDKYLAESKKQFAEYVKQIL 475
+S R Y+ L+KH + ++ E A YV +IL
Sbjct: 398 DESKRPKYKELLKHPFILMYE------ERTVDVAGYVGKIL 432
>sp|P47809|MP2K4_MOUSE Dual specificity mitogen-activated protein kinase kinase 4 (MAP
kinase kinase 4) (MAPKK 4) (MAPK/ERK kinase 4)
(JNK-activating kinase 1) (C-JUN N-terminal kinase
kinase 1) (JNK kinase 1) (JNKK 1) (SAPK/ERK kinase 1)
(SEK1)
Length = 397
Score = 147 bits (370), Expect = 3e-35
Identities = 76/162 (46%), Positives = 108/162 (66%), Gaps = 3/162 (1%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRSDVW 178
PSN L+D+ G +K+CDFGISG L++S+AKT + G +PY+APERI+P S GY +RSDVW
Sbjct: 230 PSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVW 289
Query: 179 SLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPAL--PADSVYSDTVRNFISQCLVK 352
SLG+++ ELA +FPY +WNS+FDQL VV D PP L + +S + NF++ CL K
Sbjct: 290 SLGITLYELATGRFPYPKWNSVFDQLTQVVKGD-PPQLSNSEEREFSPSFINFVNLCLTK 348
Query: 353 KDSDRASYETLIKHGMLTPFKDDKYLAESKKQFAEYVKQILN 478
+S R Y+ L+KH + ++ E + A YV +IL+
Sbjct: 349 DESKRPKYKELLKHPFILMYE------ERTVEVACYVCKILD 384
>sp|P45985|MP2K4_HUMAN Dual specificity mitogen-activated protein kinase kinase 4 (MAP
kinase kinase 4) (JNK-activating kinase 1) (c-Jun
N-terminal kinase kinase 1) (JNKK) (SAPK/ERK kinase 1)
(SEK1)
Length = 399
Score = 147 bits (370), Expect = 3e-35
Identities = 76/162 (46%), Positives = 108/162 (66%), Gaps = 3/162 (1%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRSDVW 178
PSN L+D+ G +K+CDFGISG L++S+AKT + G +PY+APERI+P S GY +RSDVW
Sbjct: 232 PSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSDVW 291
Query: 179 SLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPAL--PADSVYSDTVRNFISQCLVK 352
SLG+++ ELA +FPY +WNS+FDQL VV D PP L + +S + NF++ CL K
Sbjct: 292 SLGITLYELATGRFPYPKWNSVFDQLTQVVKGD-PPQLSNSEEREFSPSFINFVNLCLTK 350
Query: 353 KDSDRASYETLIKHGMLTPFKDDKYLAESKKQFAEYVKQILN 478
+S R Y+ L+KH + ++ E + A YV +IL+
Sbjct: 351 DESKRPKYKELLKHPFILMYE------ERAVEVACYVCKILD 386
>sp|O09110|MP2K3_MOUSE Dual specificity mitogen-activated protein kinase kinase 3 (MAP
kinase kinase 3) (MAPKK 3) (MAPK/ERK kinase 3)
Length = 347
Score = 146 bits (368), Expect = 5e-35
Identities = 78/159 (49%), Positives = 107/159 (67%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRSDVW 178
PSN LI+K G VK+CDFGISG L++S+AKT + G +PY+APERINP+ + GY+++SDVW
Sbjct: 193 PSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVW 252
Query: 179 SLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQCLVKKD 358
SLG++++E+AI +FPY W + F QLK VV + P LPAD +S +F SQCL K
Sbjct: 253 SLGITMIEMAILRFPYESWGTPFQQLKQVV-EEPSPQLPADQ-FSPEFVDFTSQCLRKNP 310
Query: 359 SDRASYETLIKHGMLTPFKDDKYLAESKKQFAEYVKQIL 475
++R SY L++H T K K A +VK+IL
Sbjct: 311 AERMSYLELMEHPFFTLHK------TKKTDIAAFVKEIL 343
>sp|P46734|MP2K3_HUMAN Dual specificity mitogen-activated protein kinase kinase 3 (MAP
kinase kinase 3) (MAPKK 3) (MAPK/ERK kinase 3)
Length = 347
Score = 144 bits (364), Expect = 1e-34
Identities = 77/159 (48%), Positives = 107/159 (67%), Gaps = 1/159 (0%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRSDVW 178
PSN LI+K G VK+CDFGISG L++S+AKT + G +PY+APERINP+ + GY+++SDVW
Sbjct: 193 PSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVW 252
Query: 179 SLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQCLVKKD 358
SLG++++E+AI +FPY W + F QLK VV + P LPAD +S +F +QCL K
Sbjct: 253 SLGITMIEMAILRFPYESWGTPFQQLKQVV-EEPSPQLPADR-FSPEFVDFTAQCLRKNP 310
Query: 359 SDRASYETLIKHGMLTPFKDDKYLAESKKQFAEYVKQIL 475
++R SY L++H T K K A +VK+IL
Sbjct: 311 AERMSYLELMEHPFFTLHK------TKKTDIAAFVKEIL 343
>sp|P70236|MP2K6_MOUSE Dual specificity mitogen-activated protein kinase kinase 6 (MAP
kinase kinase 6) (MAPKK 6) (MAPK/ERK kinase 6) (SAPKK3)
Length = 334
Score = 143 bits (361), Expect = 3e-34
Identities = 79/160 (49%), Positives = 108/160 (67%), Gaps = 2/160 (1%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRSDVW 178
PSN LI+ G+VK+CDFGISG L++S+AKT + G +PY+APERINP+ + GYS++SD+W
Sbjct: 182 PSNVLINTLGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIW 241
Query: 179 SLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQCLVKKD 358
SLG++++ELAI +FPY W + F QLK VV + P LPAD +D V +F SQCL K
Sbjct: 242 SLGITMIELAILRFPYDSWGTPFQQLKQVV-EEPSPQLPADKFSADFV-DFTSQCLKKNS 299
Query: 359 SDRASYETLIKHGMLTPFKDDKYLAESK-KQFAEYVKQIL 475
+R +Y L++H T + ESK A +VK IL
Sbjct: 300 KERPTYPELMQHPFFT-------VHESKAADVASFVKLIL 332
>sp|P52564|MP2K6_HUMAN Dual specificity mitogen-activated protein kinase kinase 6 (MAP
kinase kinase 6) (MAPKK 6) (MAPK/ERK kinase 6) (SAPKK3)
Length = 334
Score = 143 bits (360), Expect = 4e-34
Identities = 79/160 (49%), Positives = 108/160 (67%), Gaps = 2/160 (1%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKT-NVGSQPYLAPERINPDRSGAGYSIRSDVW 178
PSN LI+ G+VK+CDFGISG L++S+AKT + G +PY+APERINP+ + GYS++SD+W
Sbjct: 182 PSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIW 241
Query: 179 SLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQCLVKKD 358
SLG++++ELAI +FPY W + F QLK VV + P LPAD ++ V +F SQCL K
Sbjct: 242 SLGITMIELAILRFPYDSWGTPFQQLKQVV-EEPSPQLPADKFSAEFV-DFTSQCLKKNS 299
Query: 359 SDRASYETLIKHGMLTPFKDDKYLAESK-KQFAEYVKQIL 475
+R +Y L++H T L ESK A +VK IL
Sbjct: 300 KERPTYPELMQHPFFT-------LHESKGTDVASFVKLIL 332
>sp|Q20347|YR62_CAEEL Putative serine/threonine-protein kinase F42G10.2
Length = 363
Score = 125 bits (314), Expect = 9e-29
Identities = 71/132 (53%), Positives = 84/132 (63%), Gaps = 4/132 (3%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKTN-VGSQPYLAPERINPDRSGAGYSIRSDVW 178
PSN L+D G VK+CDFGI G L S AKT+ G QPYLAPERI S Y +RSDVW
Sbjct: 197 PSNILVDGTGAVKLCDFGICGQLEESFAKTHDAGCQPYLAPERIT---SSDKYDVRSDVW 253
Query: 179 SLGLSVLELAIRKFPYSRWNSLFDQLKDVVASDTPPALPADS---VYSDTVRNFISQCLV 349
SLG+++ E+A KFPY WNSLFDQ+ VV+ D PP L DS YS + FI+ CL
Sbjct: 254 SLGITLYEIATGKFPYQEWNSLFDQIATVVSGD-PPILHPDSDDFHYSLPLVKFINTCLT 312
Query: 350 KKDSDRASYETL 385
K R Y+TL
Sbjct: 313 KDRRHRPKYDTL 324
>sp|P08018|PBS2_YEAST Polymyxin B resistance protein kinase
Length = 668
Score = 123 bits (309), Expect = 3e-28
Identities = 71/165 (43%), Positives = 107/165 (64%), Gaps = 7/165 (4%)
Frame = +2
Query: 2 PSNTLIDKG-GRVKVCDFGISGDLINSMAKTNVGSQPYLAPERI---NPDRSGAGYSIRS 169
P+N L G VK+CDFG+SG+L+ S+AKTN+G Q Y+APERI NPDR A Y+++S
Sbjct: 488 PTNILCSANQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSLNPDR--ATYTVQS 545
Query: 170 DVWSLGLSVLELAIRKFPY--SRWNSLFDQLKDVVASDTPPALPADSVYSDTVRNFISQC 343
D+WSLGLS+LE+A+ ++PY ++++F QL +V PP LP+D SD ++F+S C
Sbjct: 546 DIWSLGLSILEMALGRYPYPPETYDNIFSQLSAIV-DGPPPRLPSDKFSSD-AQDFVSLC 603
Query: 344 LVKKDSDRASYETLIKHGMLTPFKD-DKYLAESKKQFAEYVKQIL 475
L K R +Y L +H L +++ D +++E + E +IL
Sbjct: 604 LQKIPERRPTYAALTEHPWLVKYRNQDVHMSEYITERLERRNKIL 648
>sp|P06784|STE7_YEAST Serine/threonine-protein kinase STE7
Length = 515
Score = 121 bits (303), Expect = 2e-27
Identities = 61/149 (40%), Positives = 94/149 (63%), Gaps = 7/149 (4%)
Frame = +2
Query: 2 PSNTLIDKGGRVKVCDFGISGDLINSMAKTNVGSQPYLAPERINPDRSGAGYSIRSDVWS 181
PSN LI+ G++K+CDFG+S LINS+A T VG+ Y++PERI G YSI+ DVWS
Sbjct: 334 PSNVLINSKGQIKLCDFGVSKKLINSIADTFVGTSTYMSPERI----QGNVYSIKGDVWS 389
Query: 182 LGLSVLELAIRKFPYSRWNSLFDQLKDV---VASDTPPALPADSVYSDTVRNFISQCLVK 352
LGL ++EL +FP N D + D+ + ++ P LP D +YS + +F+++C +K
Sbjct: 390 LGLMIIELVTGEFPLGGHNDTPDGILDLLQRIVNEPSPRLPKDRIYSKEMTDFVNRCCIK 449
Query: 353 KDSDRASYETLIKHGML----TPFKDDKY 427
+ +R+S L+ H ++ +P KDDK+
Sbjct: 450 NERERSSIHELLHHDLIMKYVSPSKDDKF 478
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,207,045
Number of Sequences: 369166
Number of extensions: 1399886
Number of successful extensions: 5628
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4901
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5065
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5023626210
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)