Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_P12
(843 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9SDL7|HMT1_ARATH Homocysteine S-methyltransferase 1 (S-... 100 5e-21
sp|Q9FUN0|HMT1_MAIZE Homocysteine S-methyltransferase 1 (S-... 99 1e-20
sp|Q9FUM7|HMT4_MAIZE Homocysteine S-methyltransferase 4 (S-... 96 1e-19
sp|Q9FUM9|HMT2_MAIZE Homocysteine S-methyltransferase 2 (S-... 96 2e-19
sp|Q9FUM8|HMT3_MAIZE Homocysteine S-methyltransferase 3 (S-... 94 5e-19
sp|Q8LAX0|HMT3_ARATH Homocysteine S-methyltransferase 3 (S-... 91 4e-18
sp|Q47690|MMUM_ECOLI Homocysteine S-methyltransferase (S-me... 89 2e-17
sp|Q9M1W4|HMT2_ARATH Homocysteine S-methyltransferase 2 (S-... 87 4e-17
sp|P56707|SMTA_ASTBI Selenocysteine methyltransferase (SECY... 79 1e-14
sp|Q12525|MHT1_YEAST Homocysteine S-methyltransferase 1 (S-... 49 2e-05
>sp|Q9SDL7|HMT1_ARATH Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
(AtHMT-1)
Length = 326
Score = 100 bits (249), Expect = 5e-21
Identities = 65/172 (37%), Positives = 103/172 (59%), Gaps = 6/172 (3%)
Frame = +3
Query: 186 VRFLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLANDEILKY--QSSKVE 359
+ +L AGA+++ + SYQA I S +SIE+++ L+ SV LA + ++ + SKV
Sbjct: 60 MEYLEAGADIVVTSSYQAT-IPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVS 118
Query: 360 RTSI---MSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAF 530
S + AASIGSYGA+L+D SEY+G Y + VS LK ++ ++ L+ PD+LAF
Sbjct: 119 GHSYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAF 178
Query: 531 ETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQ 683
ET+P L+ + ++L+ E AWI TS+DG GE ++ +E +N+
Sbjct: 179 ETIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNK 230
>sp|Q9FUN0|HMT1_MAIZE Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
(ZmHMT-1)
Length = 323
Score = 99.4 bits (246), Expect = 1e-20
Identities = 63/171 (36%), Positives = 100/171 (58%), Gaps = 6/171 (3%)
Frame = +3
Query: 186 VRFLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLAN---DEILK--YQSS 350
+++L AGA+VI S SYQA I + MS+ +A+ L+ SV LAN DE K + S
Sbjct: 54 MQYLEAGADVIISSSYQAT-IPGFIARGMSVAEAEDLLRTSVKLANEARDEFWKSTLRKS 112
Query: 351 KVERTSIMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAF 530
K + AASIGSYGA+L+D SEY+GSY ++ LK ++ ++ L PD++AF
Sbjct: 113 KPIYNRALVAASIGSYGAYLADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAF 172
Query: 531 ETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYIN 680
E +P ++ + +++L+ E +WI +S+DG + GE D ++ +N
Sbjct: 173 EAIPNQMEAQALVELLEEEKVQIPSWICFSSVDGKNLCSGESFADCLKILN 223
>sp|Q9FUM7|HMT4_MAIZE Homocysteine S-methyltransferase 4 (S-methylmethionine:homocysteine
methyltransferase 4) (SMM:Hcy S-methyltransferase 4)
(ZmHMT-4)
Length = 342
Score = 95.9 bits (237), Expect = 1e-19
Identities = 59/163 (36%), Positives = 97/163 (59%), Gaps = 6/163 (3%)
Frame = +3
Query: 192 FLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLANDEILKY-----QSSKV 356
+L AGA+VI S SYQA I S S +++++L+ SV +A + + +SS+
Sbjct: 66 YLEAGADVIISASYQAT-IEGFQSRGFSRDESEELLRRSVHVAQEARRVFAAEGDRSSRR 124
Query: 357 ERTSIMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFET 536
R + AAS+GSYGA+ +D SEY+G Y ++K DLK ++ ++ L PD++AFET
Sbjct: 125 GRPPALVAASVGSYGAYRADGSEYSGDYGKSMTKEDLKNFHRRRLQVLAGAGPDLIAFET 184
Query: 537 MPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDD 662
+P L+ ++ +L+ E AW S TS DG++ A G+ +++
Sbjct: 185 IPNKLEAQVYAELLEENGIRIPAWFSFTSKDGVNAASGDPINE 227
>sp|Q9FUM9|HMT2_MAIZE Homocysteine S-methyltransferase 2 (S-methylmethionine:homocysteine
methyltransferase 2) (SMM:Hcy S-methyltransferase 2)
(ZmHMT-2)
Length = 339
Score = 95.5 bits (236), Expect = 2e-19
Identities = 58/161 (36%), Positives = 92/161 (57%), Gaps = 6/161 (3%)
Frame = +3
Query: 192 FLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQSSKVERTS- 368
+L AGA +I + SYQA I S S E ++ L+ SV +A + + +E+++
Sbjct: 65 YLEAGANIIITASYQAT-IQGFESKGFSKEQSENLLTKSVEIALEAREMFLKEHLEKSTP 123
Query: 369 ----IMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFET 536
++ AAS+GSYGA+L+D SEY+G Y + +K LK ++ ++ L PD++AFET
Sbjct: 124 IQHPVLVAASLGSYGAYLADGSEYSGDYGEAGTKEFLKDFHRRRLQVLAEAGPDLIAFET 183
Query: 537 MPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELL 656
+P L+ E ++L+ E N AW S S DG+H G+ L
Sbjct: 184 IPNKLEAEAYVELLEECNINIPAWFSFNSKDGVHIVSGDSL 224
>sp|Q9FUM8|HMT3_MAIZE Homocysteine S-methyltransferase 3 (S-methylmethionine:homocysteine
methyltransferase 3) (SMM:Hcy S-methyltransferase 3)
(ZmHMT-3)
Length = 338
Score = 94.0 bits (232), Expect = 5e-19
Identities = 56/161 (34%), Positives = 93/161 (57%), Gaps = 6/161 (3%)
Frame = +3
Query: 192 FLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQSSKVERTS- 368
+L AGA +I + SYQA I S S E ++ L+ SV +A + + +E+++
Sbjct: 65 YLEAGANIIITASYQAT-IQGFESKGFSKEQSENLLTKSVQIALEAREMFLKEHLEKSTP 123
Query: 369 ----IMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFET 536
I+ AA++GSYGA+L+D SEY+G Y + +K LK ++ ++ L PD++AFET
Sbjct: 124 IQHPILVAAALGSYGAYLADGSEYSGDYGEAGTKEFLKDFHRRRLQVLAEAGPDLIAFET 183
Query: 537 MPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELL 656
+P L+ + ++L+ E N +W+S S DG+H G+ L
Sbjct: 184 IPNKLEAQAYVELLEECNINIPSWLSFNSKDGVHVVSGDSL 224
>sp|Q8LAX0|HMT3_ARATH Homocysteine S-methyltransferase 3 (S-methylmethionine:homocysteine
methyltransferase 3) (SMM:Hcy S-methyltransferase 3)
(AtHMT-3)
Length = 347
Score = 90.9 bits (224), Expect = 4e-18
Identities = 63/173 (36%), Positives = 94/173 (54%), Gaps = 13/173 (7%)
Frame = +3
Query: 192 FLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLAND--EILKYQ------- 344
+L +GA +I + SYQA I + +S+ +A+ L+ SV + + EI +
Sbjct: 65 YLESGANIIITASYQAT-IQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWD 123
Query: 345 ---SSKVERTSIMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKP 515
+ K R I+ AAS+GSYGA+L+D SEY+G Y D VSK LK ++ ++ L
Sbjct: 124 FAYAGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGA 183
Query: 516 DILAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIE 671
D++AFET+P L+ E DL+ E + AW S TS DG+ G D V+E
Sbjct: 184 DLIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRG---DSVVE 233
>sp|Q47690|MMUM_ECOLI Homocysteine S-methyltransferase (S-methylmethionine:homocysteine
methyltransferase)
Length = 310
Score = 88.6 bits (218), Expect = 2e-17
Identities = 53/162 (32%), Positives = 83/162 (51%)
Frame = +3
Query: 201 AGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQSSKVERTSIMSA 380
AGA+ + SYQA P ++ + +K L+ SV LA Y + + +++ A
Sbjct: 61 AGAQCAITASYQATPAGFAAR-GLDEAQSKALIGKSVELARKAREAYLAENPQAGTLLVA 119
Query: 381 ASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDILAFETMPAMLDVE 560
S+G YGA+L+D SEY G Y V F + ++ ++ LL D+LA ET+P ++E
Sbjct: 120 GSVGPYGAYLADGSEYRGDYHCSVEAF--QAFHRPRVEALLDAGADLLACETLPNFSEIE 177
Query: 561 LIIDLMREYPNTSAWISMTSLDGIHTAHGELLDDVIEYINQY 686
+ +L+ YP AW S T D H + G L DV+ + Y
Sbjct: 178 ALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAGY 219
>sp|Q9M1W4|HMT2_ARATH Homocysteine S-methyltransferase 2 (S-methylmethionine:homocysteine
methyltransferase 2) (SMM:Hcy S-methyltransferase 2)
(AtHMT-2)
Length = 333
Score = 87.4 bits (215), Expect = 4e-17
Identities = 56/170 (32%), Positives = 93/170 (54%), Gaps = 11/170 (6%)
Frame = +3
Query: 192 FLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQ-----SSKV 356
+L AGA++I+S SYQA I + S E+++ L+ SV +A + Y SS +
Sbjct: 61 YLEAGADIISSASYQAT-IQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSM 119
Query: 357 E-----RTSIMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGKPDI 521
+ + I+ AAS+GSYGA+L+D SEY+G Y D ++ LK ++ ++ L D+
Sbjct: 120 DDKILKKRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADL 179
Query: 522 LAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVI 668
+AFET+P ++ + DL+ E W S S DG++ G+ + + I
Sbjct: 180 IAFETIPNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECI 229
>sp|P56707|SMTA_ASTBI Selenocysteine methyltransferase (SECYS-methyltransferase)
(SECYS-MT)
Length = 338
Score = 79.3 bits (194), Expect = 1e-14
Identities = 54/171 (31%), Positives = 88/171 (51%), Gaps = 14/171 (8%)
Frame = +3
Query: 192 FLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLANDEILKYQSSKVERTS- 368
+L GA++I + SYQA I + S E+ + L+ SV +A + Y E +S
Sbjct: 58 YLENGADIIITASYQAT-IQGFKAKGFSDEEGEALLRRSVEIAREARDLYYQRCAESSSD 116
Query: 369 ------------IMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTLLIGK 512
I+ A S+GSYGA+L+D SE++G+Y D + LK ++ ++ L
Sbjct: 117 NGDDSRILKQRPILIAGSVGSYGAYLADGSEFSGNYGDAIKSETLKDFHRRKVQILADSG 176
Query: 513 PDILAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDD 662
D+LAFE +P L+ + DL+ E T AW + TS DG + G+ +++
Sbjct: 177 VDLLAFEAVPNKLEAQAYADLLEEENIITPAWFAFTSKDGNNVVSGDSIEE 227
>sp|Q12525|MHT1_YEAST Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
Length = 324
Score = 48.9 bits (115), Expect = 2e-05
Identities = 39/186 (20%), Positives = 84/186 (45%), Gaps = 1/186 (0%)
Frame = +3
Query: 141 QKVIEKLLNSTSKGIVRFLCAGAEVITSLSYQACPITLSSSFNMSIEDAKQLMVDSVLLA 320
+KV+E++ F+ AGA ++ +++YQA ++S + ++ A + +D ++
Sbjct: 58 RKVVEEMYRD-------FMIAGANILMTITYQANFQSISENTSIKTLAAYKRFLDKIVSF 110
Query: 321 NDEILKYQSSKVERTSIMSAASIGSYGAHLSDLSEYTGSYVDKVSKFDLKTYYEEQISTL 500
E + + + SIG + AH+S EYTG Y D +++ Q+
Sbjct: 111 TREFIGEERYLI--------GSIGPWAAHVS--CEYTGDYGPHPENIDYYGFFKPQLENF 160
Query: 501 LIGKP-DILAFETMPAMLDVELIIDLMREYPNTSAWISMTSLDGIHTAHGELLDDVIEYI 677
+ D++ FET+P +++ I+ + + +I ++ D G L+++ +I
Sbjct: 161 NQNRDIDLIGFETIPNFHELKAILSWDEDIISKPFYIGLSVDDNSLLRDGTTLEEISVHI 220
Query: 678 NQYSTK 695
K
Sbjct: 221 KGLGNK 226
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,867,455
Number of Sequences: 369166
Number of extensions: 1793776
Number of successful extensions: 4979
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4813
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4963
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)