Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_N04
(839 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q24800|SEVE_ECHGR Severin 171 3e-42
sp|P10733|SEVE_DICDI Severin 130 3e-30
sp|Q07171|GELS_DROME Gelsolin precursor 117 4e-26
sp|Q27319|GELS_HOMAM Gelsolin, cytoplasmic (Actin-depolymer... 111 3e-24
sp|O81644|VILI2_ARATH Villin-2 110 5e-24
sp|O93510|GELS_CHICK Gelsolin precursor (Actin-depolymerizi... 110 5e-24
sp|O81645|VILI3_ARATH Villin-3 106 7e-23
sp|P13020|GELS_MOUSE Gelsolin precursor (Actin-depolymerizi... 103 8e-22
sp|Q62468|VILI_MOUSE Villin-1 102 1e-21
sp|P06396|GELS_HUMAN Gelsolin precursor (Actin-depolymerizi... 102 2e-21
>sp|Q24800|SEVE_ECHGR Severin
Length = 374
Score = 171 bits (432), Expect = 3e-42
Identities = 79/143 (55%), Positives = 101/143 (70%), Gaps = 2/143 (1%)
Frame = +2
Query: 8 KAEKYDWKDSNVELIGSTEDRTVKKSSAETEKAWNAAKACKTSALFIWRIEKFKVVEWPK 187
KA+ YDWKDSN+EL GS++DR VKK SA TEK W + L +WR+ +F + P
Sbjct: 6 KAKDYDWKDSNMELFGSSKDRQVKKESAMTEKCWEPVGRATSPFLMVWRVNQFTLEPVPS 65
Query: 188 QQYGEFYEGDSYIVLNIYQKE--KELLYDVHFWIGKYSTQDEYGTAAYKTVELDTLLDDK 361
+ G FY GDSY++ + +LLY+VHFWIGK+ST DEYGTAAYKTVELDT LDD
Sbjct: 66 DEIGNFYNGDSYVICKATRSPGGDKLLYNVHFWIGKHSTADEYGTAAYKTVELDTFLDDA 125
Query: 362 PVQHREVQHFESEMFKSYFPNII 430
VQHREV+ +ES++FKSYF ++
Sbjct: 126 AVQHREVEGYESQLFKSYFDKLV 148
>sp|P10733|SEVE_DICDI Severin
Length = 362
Score = 130 bits (328), Expect = 3e-30
Identities = 65/140 (46%), Positives = 89/140 (63%)
Frame = +2
Query: 2 MKKAEKYDWKDSNVELIGSTEDRTVKKSSAETEKAWNAAKACKTSALFIWRIEKFKVVEW 181
M K K D +NV IG+ D+ + +A TE W + L IWRIE FKVV
Sbjct: 1 MIKNRKLDITSTNVAGIGTDLDKKCRLDAASTEAQWKGVG--QAPGLKIWRIENFKVVPV 58
Query: 182 PKQQYGEFYEGDSYIVLNIYQKEKELLYDVHFWIGKYSTQDEYGTAAYKTVELDTLLDDK 361
P+ YG+FY+GDSYI+L+ +++ L +D+HF++G ++TQDE GTAAYKTVELD L
Sbjct: 59 PESSYGKFYDGDSYIILHTFKEGNSLKHDIHFFLGTFTTQDEAGTAAYKTVELDDFLGGA 118
Query: 362 PVQHREVQHFESEMFKSYFP 421
P+Q+R+ Q +ES F S FP
Sbjct: 119 PIQYRQCQSYESPSFLSLFP 138
>sp|Q07171|GELS_DROME Gelsolin precursor
Length = 798
Score = 117 bits (293), Expect = 4e-26
Identities = 59/105 (56%), Positives = 75/105 (71%), Gaps = 2/105 (1%)
Frame = +2
Query: 116 AKACKTSALFIWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQ--KEKELLYDVHFWIGK 289
A A +T L IWRIE F+ V +PK YG+FY GDS+IVLN + K+K+L +DVHFW+G
Sbjct: 64 ANAGRTPGLEIWRIENFEPVIYPKTNYGKFYTGDSFIVLNTIENKKDKKLSWDVHFWLGL 123
Query: 290 YSTQDEYGTAAYKTVELDTLLDDKPVQHREVQHFESEMFKSYFPN 424
++ DE G AA TV+LD LL+ PVQHREVQ ES++F SYF N
Sbjct: 124 ETSTDEAGAAAILTVQLDDLLNGGPVQHREVQDHESQLFLSYFKN 168
>sp|Q27319|GELS_HOMAM Gelsolin, cytoplasmic (Actin-depolymerizing factor) (ADF)
Length = 754
Score = 111 bits (277), Expect = 3e-24
Identities = 53/93 (56%), Positives = 67/93 (72%)
Frame = +2
Query: 140 LFIWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQKEKELLYDVHFWIGKYSTQDEYGTA 319
L IWRIE F+VV +PK++YG+FY+GDSYIVL L +D+HFW+G ++QDE GTA
Sbjct: 14 LTIWRIENFEVVPYPKEKYGQFYQGDSYIVLYTRDVNGNLSWDLHFWLGSETSQDEAGTA 73
Query: 320 AYKTVELDTLLDDKPVQHREVQHFESEMFKSYF 418
A KTVELD L PVQHREV+ E+ +F S F
Sbjct: 74 AIKTVELDDQLGGVPVQHREVEGHETSLFLSRF 106
Score = 55.5 bits (132), Expect = 2e-07
Identities = 31/75 (41%), Positives = 42/75 (56%)
Frame = +2
Query: 194 YGEFYEGDSYIVLNIYQKEKELLYDVHFWIGKYSTQDEYGTAAYKTVELDTLLDDKPVQH 373
+G F+ GDSY++ IY+ Y ++FW G S+QDE ++A TV LD L K VQ
Sbjct: 424 HGFFFGGDSYVLKYIYEVNGNERYILYFWQGCASSQDEKASSAIHTVRLDNELCGKAVQV 483
Query: 374 REVQHFESEMFKSYF 418
R VQ +E F F
Sbjct: 484 RVVQGYEPAHFLRIF 498
>sp|O81644|VILI2_ARATH Villin-2
Length = 976
Score = 110 bits (275), Expect = 5e-24
Identities = 55/99 (55%), Positives = 69/99 (69%), Gaps = 1/99 (1%)
Frame = +2
Query: 146 IWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQ-KEKELLYDVHFWIGKYSTQDEYGTAA 322
IWRIE F+ V PK ++G+FY GD+YIVL Q K L+D+HFWIGK ++QDE GTAA
Sbjct: 21 IWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKDTSQDEAGTAA 80
Query: 323 YKTVELDTLLDDKPVQHREVQHFESEMFKSYFPNIIFSI 439
KTVELD +L + VQHRE+Q ES+ F SYF I +
Sbjct: 81 VKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPL 119
Score = 46.2 bits (108), Expect = 1e-04
Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 1/98 (1%)
Frame = +2
Query: 140 LFIWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQK-EKELLYDVHFWIGKYSTQDEYGT 316
L +W + PK+ G+ Y GD Y+VL Y E++ Y + W GK S ++ T
Sbjct: 392 LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQDT 451
Query: 317 AAYKTVELDTLLDDKPVQHREVQHFESEMFKSYFPNII 430
A + L +PVQ R + E F + F ++
Sbjct: 452 AIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQPMV 489
>sp|O93510|GELS_CHICK Gelsolin precursor (Actin-depolymerizing factor) (ADF) (Brevin)
(Homogenin)
Length = 778
Score = 110 bits (275), Expect = 5e-24
Identities = 57/102 (55%), Positives = 68/102 (66%), Gaps = 1/102 (0%)
Frame = +2
Query: 116 AKACKTSALFIWRIEKFKVVEWPKQQYGEFYEGDSYIVLN-IYQKEKELLYDVHFWIGKY 292
+KA K L IWRIEKF +V PK YG+F+ GDSY+VLN I Q+ L YD+HFW+G
Sbjct: 56 SKAGKEPGLQIWRIEKFDLVPVPKNLYGDFFTGDSYLVLNTIRQRSGNLQYDLHFWLGDE 115
Query: 293 STQDEYGTAAYKTVELDTLLDDKPVQHREVQHFESEMFKSYF 418
S+QDE G AA TV++D L K VQHREVQ ES F YF
Sbjct: 116 SSQDERGAAAIFTVQMDDYLQGKAVQHREVQGHESSTFLGYF 157
Score = 71.2 bits (173), Expect = 3e-12
Identities = 40/91 (43%), Positives = 51/91 (56%)
Frame = +2
Query: 146 IWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQKEKELLYDVHFWIGKYSTQDEYGTAAY 325
IWRIE + V YG+FY GDSYI+L Y+ + ++ W G +STQDE T+A+
Sbjct: 445 IWRIEGSEKVPVDPATYGQFYGGDSYIILYDYRHAGKQGQIIYTWQGAHSTQDEIATSAF 504
Query: 326 KTVELDTLLDDKPVQHREVQHFESEMFKSYF 418
TV+LD L PVQ R VQ E S F
Sbjct: 505 LTVQLDEELGGSPVQKRVVQGKEPPHLMSMF 535
>sp|O81645|VILI3_ARATH Villin-3
Length = 966
Score = 106 bits (265), Expect = 7e-23
Identities = 54/99 (54%), Positives = 68/99 (68%), Gaps = 1/99 (1%)
Frame = +2
Query: 146 IWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQ-KEKELLYDVHFWIGKYSTQDEYGTAA 322
IWRIE F+ V PK ++G+FY GD+YIVL Q K L+D+HFWIGK ++QDE GTAA
Sbjct: 23 IWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKDTSQDEAGTAA 82
Query: 323 YKTVELDTLLDDKPVQHREVQHFESEMFKSYFPNIIFSI 439
KTVELD L + VQ+RE+Q ES+ F SYF I +
Sbjct: 83 VKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPL 121
Score = 47.4 bits (111), Expect = 5e-05
Identities = 29/98 (29%), Positives = 45/98 (45%), Gaps = 1/98 (1%)
Frame = +2
Query: 140 LFIWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQK-EKELLYDVHFWIGKYSTQDEYGT 316
L +W I+ K G+ Y GD Y+VL Y E++ Y + W GK S Q++ T
Sbjct: 394 LEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQET 453
Query: 317 AAYKTVELDTLLDDKPVQHREVQHFESEMFKSYFPNII 430
A + L +PVQ R + E F + F +++
Sbjct: 454 AVRLASTMTNSLKGRPVQARIFEGKEPPQFVALFQHMV 491
>sp|P13020|GELS_MOUSE Gelsolin precursor (Actin-depolymerizing factor) (ADF) (Brevin)
Length = 780
Score = 103 bits (256), Expect = 8e-22
Identities = 50/101 (49%), Positives = 65/101 (64%), Gaps = 1/101 (0%)
Frame = +2
Query: 119 KACKTSALFIWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQ-KEKELLYDVHFWIGKYS 295
KA K L IWR+EKF +V P YG+F+ GD+Y++L Q + L YD+H+W+G
Sbjct: 59 KAGKEPGLQIWRVEKFDLVPVPPNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNEC 118
Query: 296 TQDEYGTAAYKTVELDTLLDDKPVQHREVQHFESEMFKSYF 418
+QDE G AA TV+LD L+ + VQHREVQ FES F YF
Sbjct: 119 SQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESSTFSGYF 159
Score = 64.3 bits (155), Expect = 4e-10
Identities = 42/102 (41%), Positives = 54/102 (52%), Gaps = 6/102 (5%)
Frame = +2
Query: 146 IWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQ---KEKELLYDVHFWIGKYSTQDEYGT 316
IWRIE V YG+FY GDSYI+L Y+ ++ +++Y+ W G STQDE
Sbjct: 447 IWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYN---WQGAQSTQDEVAA 503
Query: 317 AAYKTVELDTLLDDKPVQHREVQHFESEMFKSYF---PNIIF 433
+A T +LD L PVQ R VQ E S F P II+
Sbjct: 504 SAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIY 545
>sp|Q62468|VILI_MOUSE Villin-1
Length = 827
Score = 102 bits (255), Expect = 1e-21
Identities = 47/96 (48%), Positives = 64/96 (66%)
Frame = +2
Query: 131 TSALFIWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQKEKELLYDVHFWIGKYSTQDEY 310
T + IWRIE ++V P +G F++GD Y+VL I++ L YD+H+WIG+ S+QDE
Sbjct: 16 TPGIQIWRIEAMQMVPVPSSTFGSFFDGDCYVVLAIHKTSSTLSYDIHYWIGQDSSQDEQ 75
Query: 311 GTAAYKTVELDTLLDDKPVQHREVQHFESEMFKSYF 418
G AA T ++D L + VQHREVQ ESE F+SYF
Sbjct: 76 GAAAIYTTQMDDYLKGRAVQHREVQGNESETFRSYF 111
Score = 60.8 bits (146), Expect = 4e-09
Identities = 32/91 (35%), Positives = 48/91 (52%)
Frame = +2
Query: 146 IWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQKEKELLYDVHFWIGKYSTQDEYGTAAY 325
+WRIE ++V + G FY GD Y++L Y ++ Y ++ W G ++QDE +AY
Sbjct: 402 VWRIEDLELVPVESKWLGHFYGGDCYLLLYTYLIGEKQHYLLYIWQGSQASQDEIAASAY 461
Query: 326 KTVELDTLLDDKPVQHREVQHFESEMFKSYF 418
+ V LD +D+PVQ R E S F
Sbjct: 462 QAVLLDQKYNDEPVQIRVTMGKEPPHLMSIF 492
>sp|P06396|GELS_HUMAN Gelsolin precursor (Actin-depolymerizing factor) (ADF) (Brevin)
(AGEL)
Length = 782
Score = 102 bits (253), Expect = 2e-21
Identities = 50/101 (49%), Positives = 65/101 (64%), Gaps = 1/101 (0%)
Frame = +2
Query: 119 KACKTSALFIWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQ-KEKELLYDVHFWIGKYS 295
KA K L IWR+EKF +V P YG+F+ GD+Y++L Q + L YD+H+W+G
Sbjct: 61 KAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNEC 120
Query: 296 TQDEYGTAAYKTVELDTLLDDKPVQHREVQHFESEMFKSYF 418
+QDE G AA TV+LD L+ + VQHREVQ FES F YF
Sbjct: 121 SQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYF 161
Score = 64.3 bits (155), Expect = 4e-10
Identities = 42/102 (41%), Positives = 54/102 (52%), Gaps = 6/102 (5%)
Frame = +2
Query: 146 IWRIEKFKVVEWPKQQYGEFYEGDSYIVLNIYQ---KEKELLYDVHFWIGKYSTQDEYGT 316
IWRIE V YG+FY GDSYI+L Y+ ++ +++Y+ W G STQDE
Sbjct: 449 IWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYN---WQGAQSTQDEVAA 505
Query: 317 AAYKTVELDTLLDDKPVQHREVQHFESEMFKSYF---PNIIF 433
+A T +LD L PVQ R VQ E S F P II+
Sbjct: 506 SAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIY 547
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,083,336
Number of Sequences: 369166
Number of extensions: 1611050
Number of successful extensions: 4234
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4055
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4208
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8197207050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)