Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_M20
(913 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99816|TS101_HUMAN Tumor susceptibility gene 101 protein 196 8e-50
sp|Q61187|TS101_MOUSE Tumor susceptibility gene 101 protein 187 3e-47
sp|Q6IRE4|TS101_RAT Tumor susceptibility gene 101 protein 186 8e-47
sp|P25604|STP22_YEAST Suppressor protein STP22 of temperatu... 47 6e-05
sp|Q9NR09|BIRC6_HUMAN Baculoviral IAP repeat-containing pro... 37 0.077
sp|Q05854|YL78_YEAST Putative transcriptional regulatory pr... 33 0.85
sp|O78483|RPOC2_GUITH DNA-directed RNA polymerase beta'' ch... 32 1.9
sp|Q6AY08|AL2SB_RAT Amyotrophic lateral sclerosis 2 chromos... 32 3.2
sp|P42749|UBC5_ARATH Ubiquitin-conjugating enzyme E2-21 kDa... 31 4.2
sp|P42748|UBC4_ARATH Ubiquitin-conjugating enzyme E2-21 kDa... 31 4.2
>sp|Q99816|TS101_HUMAN Tumor susceptibility gene 101 protein
Length = 390
Score = 196 bits (498), Expect = 8e-50
Identities = 106/282 (37%), Positives = 151/282 (53%), Gaps = 1/282 (0%)
Frame = +3
Query: 3 YTCKDRVKREVQDTINVYPDLSPKMDNYVFTDGVQRTLISLGGTIKVYYMGQTYNIPVSV 182
Y +D RE + I +Y DL P +D+YVF DG R L++L GTI V Y G TYNIP+ +
Sbjct: 15 YKYRDLTVRETVNVITLYKDLKPVLDSYVFNDGSSRELMNLTGTIPVPYRGNTYNIPICL 74
Query: 183 YLPETYPYKAPMPYVKPTSTMQIKPNEHVDNQGRIYLPYLNEWSESKSDIQGLLSIMQIE 362
+L +TYPY P+ +VKPTS+M IK +HVD G+IYLPYL+EW +SD+ GL+ +M +
Sbjct: 75 WLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHEWKHPQSDLLGLIQVMIVV 134
Query: 363 FGERPPVFSKSASQPY-PVATTNPPXXXXXXXXXXXXXXXXXXXXXNQHGIGSGWTNSNQ 539
FG+ PPVFS+ S Y P T PP N G
Sbjct: 135 FGDEPPVFSRPISASYPPYQATGPPNTSYMPGMPGGISPYPSGYPPNPSGYPGCPYPPGG 194
Query: 540 PYPPATMPTNQYIPGVVNSNPYQPAITSNSVSNSAVINDDVYKMSILSAVEDKIHRMYAD 719
PYP T ++QY P QP +T+ S I++D + S++SAV DK+ +
Sbjct: 195 PYPATT--SSQY--------PSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKE 244
Query: 720 IYAQNSAEIDVLENTNKDLKKGFDTIRRYIENMDKDTINVKK 845
+ AE++ L+ T +DLKKG + + +D++ V K
Sbjct: 245 EMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDK 286
>sp|Q61187|TS101_MOUSE Tumor susceptibility gene 101 protein
Length = 391
Score = 187 bits (476), Expect = 3e-47
Identities = 105/284 (36%), Positives = 149/284 (52%), Gaps = 3/284 (1%)
Frame = +3
Query: 3 YTCKDRVKREVQDTINVYPDLSPKMDNYVFTDGVQRTLISLGGTIKVYYMGQTYNIPVSV 182
Y +D R+ + I +Y DL P +D+YVF DG R L++L GTI V Y G YNIP+ +
Sbjct: 15 YKYRDLTVRQTVNVIAMYKDLKPVLDSYVFNDGSSRELVNLTGTIPVRYRGNIYNIPICL 74
Query: 183 YLPETYPYKAPMPYVKPTSTMQIKPNEHVDNQGRIYLPYLNEWSESKSDIQGLLSIMQIE 362
+L +TYPY P+ +VKPTS+M IK +HVD G+IYLPYL++W +S++ L+ IM +
Sbjct: 75 WLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHDWKHPRSELLELIQIMIVI 134
Query: 363 FGERPPVFSK---SASQPYPVATTNPPXXXXXXXXXXXXXXXXXXXXXNQHGIGSGWTNS 533
FGE PPVFS+ SAS P P T PP N G
Sbjct: 135 FGEEPPVFSRPTVSASYP-PYTATGPPNTSYMPGMPSGISAYPSGYPPNPSGY------P 187
Query: 534 NQPYPPATMPTNQYIPGVVNSNPYQPAITSNSVSNSAVINDDVYKMSILSAVEDKIHRMY 713
PYPPA Y + P QP +T+ S I++D + S++SAV DK+
Sbjct: 188 GCPYPPA----GPYPATTSSQYPSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRM 243
Query: 714 ADIYAQNSAEIDVLENTNKDLKKGFDTIRRYIENMDKDTINVKK 845
+ AE++ L+ T +DLKKG + + +D++ V K
Sbjct: 244 KEEMDGAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDK 287
>sp|Q6IRE4|TS101_RAT Tumor susceptibility gene 101 protein
Length = 391
Score = 186 bits (472), Expect = 8e-47
Identities = 104/284 (36%), Positives = 148/284 (52%), Gaps = 3/284 (1%)
Frame = +3
Query: 3 YTCKDRVKREVQDTINVYPDLSPKMDNYVFTDGVQRTLISLGGTIKVYYMGQTYNIPVSV 182
Y +D R+ + I +Y DL P +D+YVF DG R L++L GTI V Y G YNIP+ +
Sbjct: 15 YKYRDLTVRQTVNVIAMYKDLKPVLDSYVFNDGSSRELVNLTGTIPVRYRGNIYNIPICL 74
Query: 183 YLPETYPYKAPMPYVKPTSTMQIKPNEHVDNQGRIYLPYLNEWSESKSDIQGLLSIMQIE 362
+L +TYPY P+ +VKPTS+M IK +HVD G+IYLPYL++W +S++ L+ IM +
Sbjct: 75 WLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHDWKHPRSELLELIQIMIVI 134
Query: 363 FGERPPVFSK---SASQPYPVATTNPPXXXXXXXXXXXXXXXXXXXXXNQHGIGSGWTNS 533
FGE PPVFS+ SAS P P PP N G
Sbjct: 135 FGEEPPVFSRPTVSASYP-PYTAAGPPNTSYLPSMPSGISAYPSGYPPNPSGY------P 187
Query: 534 NQPYPPATMPTNQYIPGVVNSNPYQPAITSNSVSNSAVINDDVYKMSILSAVEDKIHRMY 713
PYPPA Y + P QP +T+ S I++D + S++SAV DK+
Sbjct: 188 GCPYPPA----GPYPATTSSQYPSQPPVTTAGPSRDGTISEDTIRASLISAVSDKLRWRM 243
Query: 714 ADIYAQNSAEIDVLENTNKDLKKGFDTIRRYIENMDKDTINVKK 845
+ AE++ L+ T +DLKKG + + +D++ V K
Sbjct: 244 KEEMDGAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDK 287
>sp|P25604|STP22_YEAST Suppressor protein STP22 of temperature-sensitive alpha-factor
receptor and arginine permease (Vacuolar protein
sorting-associated protein VPS23)
Length = 385
Score = 47.4 bits (111), Expect = 6e-05
Identities = 34/131 (25%), Positives = 61/131 (46%), Gaps = 11/131 (8%)
Frame = +3
Query: 54 YPDLSPKMDNYVFTDGVQRTLISLGGTIKVYYMGQT-YNIPVSVYLPETYPYKAPMPYVK 230
+ L P+ + +DG + L+S+ GTI G + ++IPV +++P YP K P +
Sbjct: 42 FHSLRPRTRVFTHSDGTPQLLLSIYGTISTGEDGSSPHSIPVIMWVPSMYPVKPPFISIN 101
Query: 231 ------PTSTMQIKPNEHVDNQGRIYLPYLNEWSESKSD----IQGLLSIMQIEFGERPP 380
T + + E++D+ G I LP L+ W + + +Q L+S++ PP
Sbjct: 102 LENFDMNTISSSLPIQEYIDSNGWIALPILHCWDPAAMNLIMVVQELMSLL-----HEPP 156
Query: 381 VFSKSASQPYP 413
+ P P
Sbjct: 157 QDQAPSLPPKP 167
>sp|Q9NR09|BIRC6_HUMAN Baculoviral IAP repeat-containing protein 6 (Ubiquitin-conjugating
BIR-domain enzyme apollon)
Length = 4829
Score = 37.0 bits (84), Expect = 0.077
Identities = 31/147 (21%), Positives = 58/147 (39%), Gaps = 17/147 (11%)
Frame = +3
Query: 18 RVKREVQDTINVYPDLSPKMDNYVFTDGVQRTL----ISLGGTIKVYYMGQTYNIPVSVY 185
R +R Q+ + + L + VF + L + + G Y + VY
Sbjct: 4546 RARRLAQEAVTLSTSLPLSSSSSVFVRCDEERLDIMKVLITGPADTPYANGCFEF--DVY 4603
Query: 186 LPETYPYKAPMPYVKPTSTMQIKPNEHVDNQGRIYLPYLNEW---------SESKSDIQG 338
P+ YP P+ ++ T ++ N ++ N G++ L LN W ++ S +Q
Sbjct: 4604 FPQDYPSSPPLVNLETTGGHSVRFNPNLYNDGKVCLSILNTWHGRPEEKWNPQTSSFLQV 4663
Query: 339 LLSIMQIEFGERP----PVFSKSASQP 407
L+S+ + P P + +S P
Sbjct: 4664 LVSVQSLILVAEPYFNEPGYERSRGTP 4690
>sp|Q05854|YL78_YEAST Putative transcriptional regulatory protein YLR278C
Length = 1341
Score = 33.5 bits (75), Expect = 0.85
Identities = 25/90 (27%), Positives = 33/90 (36%)
Frame = +3
Query: 387 SKSASQPYPVATTNPPXXXXXXXXXXXXXXXXXXXXXNQHGIGSGWTNSNQPYPPATMPT 566
+ S S P P+A T P Q S Q + + T
Sbjct: 1046 ASSHSDPIPIAMTQSPTPYPQTSNMLPQQHVSRPLPQQQREQPQQHITSPQRFSESNF-T 1104
Query: 567 NQYIPGVVNSNPYQPAITSNSVSNSAVIND 656
NQ G++NSNP Q AI SN S + + D
Sbjct: 1105 NQLNNGMINSNPLQSAIFSNHTSENKQLRD 1134
>sp|O78483|RPOC2_GUITH DNA-directed RNA polymerase beta'' chain (PEP) (Plastid-encoded RNA
polymerase beta'' subunit) (RNA polymerase beta''
subunit)
Length = 1286
Score = 32.3 bits (72), Expect = 1.9
Identities = 28/103 (27%), Positives = 48/103 (46%), Gaps = 1/103 (0%)
Frame = +3
Query: 123 LGGTIKVYYMGQTYNIPVS-VYLPETYPYKAPMPYVKPTSTMQIKPNEHVDNQGRIYLPY 299
L G++ GQ + I + + L + YPY V + +QIK +E V + +
Sbjct: 916 LAGSVIASESGQVFEITKNEIILRKAYPY-----LVSAGAILQIKDHELVQRNDTLAI-L 969
Query: 300 LNEWSESKSDIQGLLSIMQIEFGERPPVFSKSASQPYPVATTN 428
+ E S++ +QGL I +I +P K + +P + TTN
Sbjct: 970 VFERSKTGDIVQGLPRIEEILEARKPKEPCKLSQKPGKINTTN 1012
>sp|Q6AY08|AL2SB_RAT Amyotrophic lateral sclerosis 2 chromosomal region candidate gene
protein 12 homolog
Length = 411
Score = 31.6 bits (70), Expect = 3.2
Identities = 22/84 (26%), Positives = 43/84 (51%), Gaps = 8/84 (9%)
Frame = +3
Query: 630 VSNSAVINDDVYKMSILSAVEDKIHRMYADIYAQNSAEIDV--------LENTNKDLKKG 785
+S S +I+D ++S LSA+ ++++R + S E+D+ E +++LK+
Sbjct: 121 ISRSDIISDLEEQISELSAIIEQMNRDHQSAQKLLSNEMDLRCADMKQKFETESRELKQT 180
Query: 786 FDTIRRYIENMDKDTINVKKLVTQ 857
+EN K+T+ V+K Q
Sbjct: 181 HKEQLEKLENSYKETLKVEKAAAQ 204
>sp|P42749|UBC5_ARATH Ubiquitin-conjugating enzyme E2-21 kDa 2 (Ubiquitin-protein ligase
5) (Ubiquitin carrier protein 5)
Length = 185
Score = 31.2 bits (69), Expect = 4.2
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = +3
Query: 87 VFTDGVQRTLISLGGTIKVYYMGQTYNIPVSVYLPETYPYKAP 215
+ DG+Q + G Y G + I V LP+ YPYK+P
Sbjct: 24 MINDGMQEFFVEFSGPKDSIYEGGVWKIRVE--LPDAYPYKSP 64
>sp|P42748|UBC4_ARATH Ubiquitin-conjugating enzyme E2-21 kDa 1 (Ubiquitin-protein ligase
4) (Ubiquitin carrier protein 4)
Length = 187
Score = 31.2 bits (69), Expect = 4.2
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +3
Query: 96 DGVQRTLISLGGTIKVYYMGQTYNIPVSVYLPETYPYKAP 215
DG+Q + G Y G + I V LP+ YPYK+P
Sbjct: 27 DGMQEFYVEFNGPKDSLYQGGVWKIRVE--LPDAYPYKSP 64
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,948,857
Number of Sequences: 369166
Number of extensions: 1958890
Number of successful extensions: 5745
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5484
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5738
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9270587090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)