Planarian EST Database


Dr_sW_019_L02

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_019_L02
         (518 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q99PM3|TF2AA_MOUSE  Transcription initiation factor IIA s...    93   3e-19
sp|P52655|TF2AA_HUMAN  Transcription initiation factor IIA s...    93   3e-19
sp|Q5RCU0|TF2AA_PONPY  Transcription initiation factor IIA s...    93   3e-19
sp|O08949|TF2AA_RAT  Transcription initiation factor IIA sub...    93   3e-19
sp|P52654|TF2AA_DROME  Transcription initiation factor IIA s...    92   7e-19
sp|Q9UNN4|TF2AY_HUMAN  TFIIA-alpha and beta-like factor (Gen...    82   6e-16
sp|Q8R4I4|TF2AY_MOUSE  TFIIA-alpha and beta-like factor (Gen...    81   2e-15
sp|P32773|TOA1_YEAST  Transcription initiation factor IIA la...    51   2e-06
sp|Q8BIE6|FRM4A_MOUSE  FERM domain containing protein 4A           33   0.31 
sp|Q9P2Q2|FRM4A_HUMAN  FERM domain containing protein 4A           33   0.31 
>sp|Q99PM3|TF2AA_MOUSE Transcription initiation factor IIA subunit 1 (General
           transcription factor IIA1) [Contains: Transcription
           initiation factor IIA alpha chain (TFIIA p35 subunit);
           Transcription initiation factor IIA beta chain (TFIIA
           p19 subunit)]
          Length = 378

 Score = 93.2 bits (230), Expect = 3e-19
 Identities = 35/64 (54%), Positives = 54/64 (84%)
 Frame = +3

Query: 219 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 398
           PLNSEDD+SD++ +++F+ +NV+VCQY+K++R++NKWK +   GI NL G+D +F KA+G
Sbjct: 315 PLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIG 374

Query: 399 EAEW 410
           +AEW
Sbjct: 375 DAEW 378
>sp|P52655|TF2AA_HUMAN Transcription initiation factor IIA subunit 1 (General
           transcription factor IIA1) (TFIIA-42) (TFIIAL)
           [Contains: Transcription initiation factor IIA alpha
           chain (TFIIA p35 subunit); Transcription initiation
           factor IIA beta chain (TFIIA p19 subunit)]
          Length = 376

 Score = 93.2 bits (230), Expect = 3e-19
 Identities = 35/64 (54%), Positives = 54/64 (84%)
 Frame = +3

Query: 219 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 398
           PLNSEDD+SD++ +++F+ +NV+VCQY+K++R++NKWK +   GI NL G+D +F KA+G
Sbjct: 313 PLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIG 372

Query: 399 EAEW 410
           +AEW
Sbjct: 373 DAEW 376
>sp|Q5RCU0|TF2AA_PONPY Transcription initiation factor IIA subunit 1 (General
           transcription factor IIA1) [Contains: Transcription
           initiation factor IIA alpha chain (TFIIA p35 subunit);
           Transcription initiation factor IIA beta chain (TFIIA
           p19 subunit)]
          Length = 376

 Score = 93.2 bits (230), Expect = 3e-19
 Identities = 35/64 (54%), Positives = 54/64 (84%)
 Frame = +3

Query: 219 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 398
           PLNSEDD+SD++ +++F+ +NV+VCQY+K++R++NKWK +   GI NL G+D +F KA+G
Sbjct: 313 PLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIG 372

Query: 399 EAEW 410
           +AEW
Sbjct: 373 DAEW 376
>sp|O08949|TF2AA_RAT Transcription initiation factor IIA subunit 1 (General
           transcription factor IIA1) [Contains: Transcription
           initiation factor IIA alpha chain (TFIIA p35 subunit);
           Transcription initiation factor IIA beta chain (TFIIA
           p19 subunit)]
          Length = 377

 Score = 93.2 bits (230), Expect = 3e-19
 Identities = 35/64 (54%), Positives = 54/64 (84%)
 Frame = +3

Query: 219 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 398
           PLNSEDD+SD++ +++F+ +NV+VCQY+K++R++NKWK +   GI NL G+D +F KA+G
Sbjct: 314 PLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIG 373

Query: 399 EAEW 410
           +AEW
Sbjct: 374 DAEW 377
>sp|P52654|TF2AA_DROME Transcription initiation factor IIA subunit 1 (dTFIIA-L) [Contains:
           Transcription initiation factor IIA alpha chain (TFIIA
           p30 subunit); Transcription initiation factor IIA beta
           chain (TFIIA p20 subunit)]
          Length = 366

 Score = 92.0 bits (227), Expect = 7e-19
 Identities = 38/64 (59%), Positives = 51/64 (79%)
 Frame = +3

Query: 219 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 398
           PLNSEDD++D+D  ++F+ DNVIVCQY+K+ R+RNKWK     GI N++GKD VF K+ G
Sbjct: 303 PLNSEDDVTDEDSAEMFDTDNVIVCQYDKITRSRNKWKFYLKDGIMNMRGKDYVFQKSNG 362

Query: 399 EAEW 410
           +AEW
Sbjct: 363 DAEW 366
>sp|Q9UNN4|TF2AY_HUMAN TFIIA-alpha and beta-like factor (General transcription factor II
           A, 1-like factor)
          Length = 478

 Score = 82.4 bits (202), Expect = 6e-16
 Identities = 34/64 (53%), Positives = 47/64 (73%)
 Frame = +3

Query: 219 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 398
           PLNS DD+S+ D   +F+ DNVIVCQY+K++R++NKWK     G+    G+D VF KA+G
Sbjct: 415 PLNSGDDVSEQDVPDLFDTDNVIVCQYDKIHRSKNKWKFYLKDGVMCFGGRDYVFAKAIG 474

Query: 399 EAEW 410
           +AEW
Sbjct: 475 DAEW 478
>sp|Q8R4I4|TF2AY_MOUSE TFIIA-alpha and beta-like factor (General transcription factor II
           A, 1-like factor)
          Length = 468

 Score = 80.9 bits (198), Expect = 2e-15
 Identities = 33/64 (51%), Positives = 47/64 (73%)
 Frame = +3

Query: 219 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 398
           PLNS DD+S+ D   +F+ +NVIVCQY+K++R++N+WK     G+    G+D VF KA+G
Sbjct: 405 PLNSGDDVSEQDVPDLFDTENVIVCQYDKIHRSKNRWKFYLKDGVMCFGGRDYVFAKAIG 464

Query: 399 EAEW 410
           EAEW
Sbjct: 465 EAEW 468
>sp|P32773|TOA1_YEAST Transcription initiation factor IIA large chain (TFIIA 32 kDa
           subunit)
          Length = 286

 Score = 50.8 bits (120), Expect = 2e-06
 Identities = 22/62 (35%), Positives = 36/62 (58%)
 Frame = +3

Query: 225 NSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVGEA 404
           +S+DD    + E+    +N+++C Y+KV RT+ +WK +   G+  +   D  F KA  EA
Sbjct: 224 DSDDDYLISEGEEDGPDENLMLCLYDKVTRTKARWKCSLKDGVVTINRNDYTFQKAQVEA 283

Query: 405 EW 410
           EW
Sbjct: 284 EW 285
>sp|Q8BIE6|FRM4A_MOUSE FERM domain containing protein 4A
          Length = 1020

 Score = 33.5 bits (75), Expect = 0.31
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = +1

Query: 259  RKYSRQTTLSFASMRRSTELATSGSSISLVESRICRARIQCSTRRSARPSGERAVTPS 432
            +++SR +  S  S    ++ +TS  S  +  SR+ R    C    +A P  +R+ TPS
Sbjct: 921  KEHSRLSHTSSTSSDSGSQYSTSSQSTFVAHSRVTRMPQMCKATSAALPQSQRSSTPS 978
>sp|Q9P2Q2|FRM4A_HUMAN FERM domain containing protein 4A
          Length = 1024

 Score = 33.5 bits (75), Expect = 0.31
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = +1

Query: 259  RKYSRQTTLSFASMRRSTELATSGSSISLVESRICRARIQCSTRRSARPSGERAVTPS 432
            +++SR +  S  S    ++ +TS  S  +  SR+ R    C    +A P  +R+ TPS
Sbjct: 925  KEHSRLSHTSSTSSDSGSQYSTSSQSTFVAHSRVTRMPQMCKATSAALPQSQRSSTPS 982
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,730,535
Number of Sequences: 369166
Number of extensions: 654805
Number of successful extensions: 1648
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1624
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1648
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3403581975
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)