Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_J10
(679 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UI08|EVL_HUMAN Ena/vasodilator-stimulated phosphoprote... 47 4e-05
sp|P70429|EVL_MOUSE Ena/vasodilator-stimulated phosphoprote... 45 1e-04
sp|P50551|VASP_CANFA Vasodilator-stimulated phosphoprotein ... 43 9e-04
sp|P70460|VASP_MOUSE Vasodilator-stimulated phosphoprotein ... 38 0.021
sp|O97902|DDEF1_BOVIN 130-kDa phosphatidylinositol 4,5-biph... 38 0.028
sp|P16884|NFH_RAT Neurofilament triplet H protein (200 kDa ... 38 0.028
sp|P50552|VASP_HUMAN Vasodilator-stimulated phosphoprotein ... 37 0.062
sp|O07650|TONB_CAMCO Protein tonB 37 0.062
sp|Q26602|SMOX3_SCHMA Homeobox protein SMOX-3 36 0.081
sp|Q60554|NKX61_MESAU Homeobox protein Nkx-6.1 36 0.11
>sp|Q9UI08|EVL_HUMAN Ena/vasodilator-stimulated phosphoprotein-like protein
(Ena/VASP-like protein)
Length = 416
Score = 47.4 bits (111), Expect = 4e-05
Identities = 36/141 (25%), Positives = 60/141 (42%), Gaps = 4/141 (2%)
Frame = +3
Query: 141 RAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDGINP 320
R K + D E P+ +S+PP SE K R + +
Sbjct: 278 RRKAASQSDKPAEKKEDESQMEDPSTSPSPGTRAASQPPNS---SEAGRKPWERSNSVEK 334
Query: 321 PKKIEQPRTPA---TPTVNLPFKN-PNQSSKPLPSNQDPTTASFVTSADLEKFRAELLQD 488
P RTP+ +P P ++ P+ KP S D +F DL++ + E+L++
Sbjct: 335 PVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAF----DLDRMKQEILEE 390
Query: 489 IVSNMEKFKEDIINGIISKLN 551
+V + K KE+II+ I +L+
Sbjct: 391 VVRELHKVKEEIIDAIRQELS 411
>sp|P70429|EVL_MOUSE Ena/vasodilator-stimulated phosphoprotein-like protein
(Ena/VASP-like protein)
Length = 414
Score = 45.4 bits (106), Expect = 1e-04
Identities = 38/146 (26%), Positives = 66/146 (45%), Gaps = 4/146 (2%)
Frame = +3
Query: 126 LASQLRAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQ 305
LA + +A +T K + D E P+ +S+PP SE K R
Sbjct: 273 LAKRRKAASQTDK--PADRKEDESQTEDPSTSPSPGTRATSQPPNS---SEAGRKPWERS 327
Query: 306 DGINPPKKIEQPRTPA---TPTVNLPFKN-PNQSSKPLPSNQDPTTASFVTSADLEKFRA 473
+ + P RTP+ +P P ++ P+ KP S D + + DL++ +
Sbjct: 328 NSVEKPVSSLLSRTPSVAKSPEAKSPLQSQPHSRVKPAGSVNDVG----LDALDLDRMKQ 383
Query: 474 ELLQDIVSNMEKFKEDIINGIISKLN 551
E+L+++V + K KE+II+ I +L+
Sbjct: 384 EILEEVVRELHKVKEEIIDAIRQELS 409
>sp|P50551|VASP_CANFA Vasodilator-stimulated phosphoprotein (VASP)
Length = 384
Score = 42.7 bits (99), Expect = 9e-04
Identities = 37/138 (26%), Positives = 63/138 (45%), Gaps = 15/138 (10%)
Frame = +3
Query: 180 SNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDGINPPKKI---EQP--R 344
S + S P P +ST+S + + + + + + Q G PPK E+P R
Sbjct: 242 SKQEEASGGPPVPKAESTRSTGGGLME-EMNAMLARRRKATQVGEKPPKDESANEEPEAR 300
Query: 345 TPA---TPTVNLPFKNPN-------QSSKPLPSNQDPTTASFVTSADLEKFRAELLQDIV 494
P + TV P++ + SS S P+T S +DLE+ + ELL+++
Sbjct: 301 VPVPAQSETVRRPWEKNSTTLPRMKSSSSVTTSEAHPSTPSSSDESDLERVKQELLEEVR 360
Query: 495 SNMEKFKEDIINGIISKL 548
++K KE+II + +L
Sbjct: 361 KELQKVKEEIIEAFVQEL 378
>sp|P70460|VASP_MOUSE Vasodilator-stimulated phosphoprotein (VASP)
Length = 376
Score = 38.1 bits (87), Expect = 0.021
Identities = 28/104 (26%), Positives = 46/104 (44%), Gaps = 1/104 (0%)
Frame = +3
Query: 240 ISSKPPKMDFLSEIKLKTQNRQDGINPPKKIEQPRTP-ATPTVNLPFKNPNQSSKPLPSN 416
+ KPPK + S+ + P + E R P + LP SS S
Sbjct: 276 VGEKPPKDESASQ-------EESEARLPAQSEPVRRPWEKNSTTLP--RMKSSSSVTTSE 326
Query: 417 QDPTTASFVTSADLEKFRAELLQDIVSNMEKFKEDIINGIISKL 548
P+T +DLE+ + ELL+++ ++K KE+II + +L
Sbjct: 327 AHPSTPCSSDDSDLERVKQELLEEVRKELQKMKEEIIEVFVQEL 370
>sp|O97902|DDEF1_BOVIN 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1
GTPase-activating protein (PIP2-dependent ARF1 GAP)
(ADP-ribosylation factor-directed GTPase-activating
protein 1) (ARF GTPase-activating protein 1) (Development
and differentiation-enhancing factor 1)
(Differentiation-enhancing factor 1) (DEF-1)
Length = 1129
Score = 37.7 bits (86), Expect = 0.028
Identities = 23/94 (24%), Positives = 41/94 (43%)
Frame = +3
Query: 168 TSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDGINPPKKIEQPRT 347
TST+S I+ P PP +T+ P + ++ + +PP RT
Sbjct: 781 TSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLSTQTSSGSSTLSKKRSPPPPPGHKRT 840
Query: 348 PATPTVNLPFKNPNQSSKPLPSNQDPTTASFVTS 449
+ P LP PN+ + P ++ P+++S T+
Sbjct: 841 LSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTN 874
>sp|P16884|NFH_RAT Neurofilament triplet H protein (200 kDa neurofilament protein)
(Neurofilament heavy polypeptide) (NF-H)
Length = 831
Score = 37.7 bits (86), Expect = 0.028
Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 7/83 (8%)
Frame = +3
Query: 204 ESPTPPVQSTQSISSKPPKMDFLS---EIKLKTQNRQDGINPPKKIEQPRTPATPTVNLP 374
E+ +P + T++ P K + S E+K K PPKK+E+ +TPATP +
Sbjct: 616 EAKSPEKEETRTEKVAPKKEEVKSPVEEVKAK--------EPPKKVEEEKTPATPKTEVK 667
Query: 375 FKN----PNQSSKPLPSNQDPTT 431
P ++ KP ++P T
Sbjct: 668 ESKKDEAPKEAQKPKAEEKEPLT 690
>sp|P50552|VASP_HUMAN Vasodilator-stimulated phosphoprotein (VASP)
Length = 380
Score = 36.6 bits (83), Expect = 0.062
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +3
Query: 393 SSKPLPSNQDPTTASFVTSADLEKFRAELLQDIVSNMEKFKEDIINGIISKL 548
SS S P T S +DL++ + ELL+++ ++K KE+II + +L
Sbjct: 323 SSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQEL 374
>sp|O07650|TONB_CAMCO Protein tonB
Length = 232
Score = 36.6 bits (83), Expect = 0.062
Identities = 35/123 (28%), Positives = 56/123 (45%), Gaps = 4/123 (3%)
Frame = +3
Query: 153 ETMKHTSTNSN----ADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDGINP 320
ET+K N N S PT P Q+ Q S+ PK + + EIK ++ I
Sbjct: 40 ETIKENKFNMAIKHFVQNSSDMKPTQPTQTIQEPSNVQPK-EPVQEIKKIKPRKEKLIAK 98
Query: 321 PKKIEQPRTPATPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSADLEKFRAELLQDIVSN 500
PKKI P ++ P K+ N +P+ Q P +S+ + A +E L++I S
Sbjct: 99 PKKIIPPAN--AKAISQPKKDTNM-QQPIMQQQTPQASSYQSVALTS--NSEFLKEIKSA 153
Query: 501 MEK 509
+++
Sbjct: 154 IDE 156
>sp|Q26602|SMOX3_SCHMA Homeobox protein SMOX-3
Length = 288
Score = 36.2 bits (82), Expect = 0.081
Identities = 30/149 (20%), Positives = 70/149 (46%), Gaps = 13/149 (8%)
Frame = +3
Query: 141 RAKQETMKHTSTNSNADNISR---ESPTPPVQSTQSISSKPPKMDFLSEIKLKTQNRQDG 311
+ KQ ++ T T+ + R E+ P + + + ++ + + + +++ QNR+
Sbjct: 1 KRKQRRIRTTFTSLQLKELERAFQETHYPDIYTREDLALRIDLTE--ARVQVWFQNRRAK 58
Query: 312 INPPKKIEQPRTPATPTVNLPFK------NPN----QSSKPLPSNQDPTTASFVTSADLE 461
+++ QP T TP+ ++P NP+ Q + P S +P S V+ D +
Sbjct: 59 FRKTERVIQPDTTTTPSSSIPINMNNIEHNPSLTNVQLTSPDNSIYNPKLLSSVSLDDQD 118
Query: 462 KFRAELLQDIVSNMEKFKEDIINGIISKL 548
K + D + M+K++ + N +++++
Sbjct: 119 KLHKAI--DAYNTMDKYETNTTNDLLNRM 145
>sp|Q60554|NKX61_MESAU Homeobox protein Nkx-6.1
Length = 364
Score = 35.8 bits (81), Expect = 0.11
Identities = 28/99 (28%), Positives = 42/99 (42%), Gaps = 4/99 (4%)
Frame = +3
Query: 168 TSTNSNADNISRESPTPPVQSTQSISSKPPKMDFLSEIKLKTQN----RQDGINPPKKIE 335
T S++ + S SP+PP+ + KPP LS + Q GIN +
Sbjct: 45 TGPPSSSSSSSSSSPSPPLGAHNPGGLKPPAAGGLSSLSSPPQQLSAATPHGIN--DILS 102
Query: 336 QPRTPATPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSA 452
+P P LP +P+ SS S+ T+AS +A
Sbjct: 103 RPSMPVASGAALPSASPSGSSSSSSSSASATSASAAAAA 141
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,031,718
Number of Sequences: 369166
Number of extensions: 1210957
Number of successful extensions: 4419
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4054
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4360
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5733423530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)