Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_J02
(700 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q92331|VPS5_YEAST Vacuolar protein sorting-associated pr... 38 0.023
sp|P32591|SWI3_YEAST Transcription regulatory protein SWI3 ... 35 0.25
sp|P04931|ARP_PLAFA Asparagine-rich protein (AG319) (ARP) 34 0.33
sp|P47104|RAV1_YEAST Regulator of V-ATPase in vacuolar memb... 34 0.43
sp|O13433|PLC1_CANAL 1-phosphatidylinositol-4,5-bisphosphat... 33 0.73
sp|P51136|GSK3H_DICDI Glycogen synthase kinase-3 homolog (G... 33 0.95
sp|Q9V3D6|CPSB_DROME Probable cleavage and polyadenylation ... 33 0.95
sp|P34545|CBP1_CAEEL Protein cbp-1 33 0.95
sp|P0C0R4|LIP_STAES Lipase precursor (Glycerol ester hydrol... 33 0.95
sp|Q7M3S9|RNGB_DICDI Ring finger protein B (Protein rngB) 33 0.95
>sp|Q92331|VPS5_YEAST Vacuolar protein sorting-associated protein VPS5
Length = 675
Score = 38.1 bits (87), Expect = 0.023
Identities = 23/88 (26%), Positives = 46/88 (52%), Gaps = 3/88 (3%)
Frame = -1
Query: 400 DDSIEDNYPAPNTAKSLAAKFKGEPPMPIGSNQSQ--SKINRSSSVNRNVLIPNNV-TQN 230
DD+I D +PNT+KS++ + G+P + S ++Q SK N S R + + T
Sbjct: 133 DDTIYDANTSPNTSKSISGRRSGKPRILFDSARAQRNSKRNHSLKAKRTTASDDTIKTPF 192
Query: 229 VEIVSSSTPNDDWLQSRISNPNQGSENN 146
+ + + +++++ + + N+ ENN
Sbjct: 193 TDPLKKAEKENEFVEEPLDDRNERRENN 220
>sp|P32591|SWI3_YEAST Transcription regulatory protein SWI3 (SWI/SNF complex component
SWI3) (Transcription factor TYE2)
Length = 825
Score = 34.7 bits (78), Expect = 0.25
Identities = 28/120 (23%), Positives = 49/120 (40%), Gaps = 7/120 (5%)
Frame = -1
Query: 406 RHDDSIEDNYPAPNT----AKSLAAKFKGEPPMPIGSNQSQSKINRSSSVNRNVLIPNNV 239
+ D+S++D PNT ++++ K +P M SQ + S +NV +N
Sbjct: 49 QQDESLKDEATVPNTRDAESEAITVTAKQQPTMQANKLDSQETPSTEESRAQNVFGQDNE 108
Query: 238 TQNV---EIVSSSTPNDDWLQSRISNPNQGSENNPVQRTDLISSESKADINEFPEQGITR 68
+ E SS + N+ S +NP N P + D +S D+ + I +
Sbjct: 109 DSDNLFGETESSVSNNEANTPSIPTNPVDNENNKPAIKEDSTIQDSNGDVKNMEDVKIQK 168
>sp|P04931|ARP_PLAFA Asparagine-rich protein (AG319) (ARP)
Length = 537
Score = 34.3 bits (77), Expect = 0.33
Identities = 17/78 (21%), Positives = 40/78 (51%)
Frame = -1
Query: 310 SNQSQSKINRSSSVNRNVLIPNNVTQNVEIVSSSTPNDDWLQSRISNPNQGSENNPVQRT 131
+ + + IN + S+N N + NN N + + +DDW + N+ +++
Sbjct: 439 NKNNNNNINMNESINNNNTLNNNNEYNNQNNNEDEDDDDW--GELGEDKYIDINSIMKKK 496
Query: 130 DLISSESKADINEFPEQG 77
++I ++ +AD+N+ ++G
Sbjct: 497 NVILNQLEADLNDLSKKG 514
>sp|P47104|RAV1_YEAST Regulator of V-ATPase in vacuolar membrane protein 1 (Suppression of
the onset of impotence protein 3)
Length = 1357
Score = 33.9 bits (76), Expect = 0.43
Identities = 44/201 (21%), Positives = 75/201 (37%), Gaps = 18/201 (8%)
Frame = -1
Query: 598 RTDDVIDELPTPGITKNLLSLWNNIETKXXXXXXXXXXSVCGTPKKSKFTSQHNSVSFQS 419
R D++ + + +L+ W IE + +P K + S +++ +
Sbjct: 1174 RVTDILCRMGCNYLAVSLVKNWKFIERNSIPVQK-----LLKSPTKDRAYSAIGAMASEP 1228
Query: 418 FDVVRHDDSIEDNY----------PAPNTAKSLAAKFKGEPPMPIGSNQSQSKINRSSSV 269
R S+ D + P P SL F PP S S S SSS
Sbjct: 1229 ISTARMRPSLFDKFGSPSASDIESPNPKLPNSLLDDFLQPPP---NSTSSNSLAQSSSSA 1285
Query: 268 NRNVL---IPNNVTQNVEIVSSSTPNDDWLQSRISNPNQGSENNPVQRTDLISSESKADI 98
R++L + + +Q+ E ++ PND + G +N R D +S + D+
Sbjct: 1286 PRSILDEFVSPSYSQHKENLTPKAPND----------SVGETDNSENRKDKLSKDILDDL 1335
Query: 97 NEFPEQ-----GITRNLVGLF 50
+ Q IT+NL+ F
Sbjct: 1336 SSQKPQKPKKSAITKNLLDDF 1356
>sp|O13433|PLC1_CANAL 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 1
(Phosphoinositide phospholipase C) (PLC-1)
(Phospholipase C-1)
Length = 1099
Score = 33.1 bits (74), Expect = 0.73
Identities = 30/124 (24%), Positives = 53/124 (42%), Gaps = 2/124 (1%)
Frame = -1
Query: 475 GTPKKSKFTSQHNSVSFQSFDVVRHDDSIEDNYPAPNTAKSLAAKFKGEPPMPIGSNQSQ 296
G+P KS+ + +S S S+ ++ D PN L + PP P QS
Sbjct: 41 GSPPKSQLLLRKSS-SPSSYSPIKSD--------LPNIYSHLRSNDSESPPQPSPKQQSS 91
Query: 295 --SKINRSSSVNRNVLIPNNVTQNVEIVSSSTPNDDWLQSRISNPNQGSENNPVQRTDLI 122
S + SSS N N+ + + ++ S+ N D +S +SN ++ T +
Sbjct: 92 LSSSSSSSSSSNTKSSTTKNIFKKLLRINKSSDNIDESRSIVSNNGGSPMSDSTTVTSTL 151
Query: 121 SSES 110
S+++
Sbjct: 152 STDT 155
>sp|P51136|GSK3H_DICDI Glycogen synthase kinase-3 homolog (GSK-3)
Length = 467
Score = 32.7 bits (73), Expect = 0.95
Identities = 29/89 (32%), Positives = 42/89 (47%), Gaps = 9/89 (10%)
Frame = -1
Query: 370 PNTAKSLA---AKFKGEPPMPIGSNQSQSKINRSSSVNRNVLIPNNV-----TQNVEIVS 215
P AK+L A + E P P+ N + S N+SSS N N + +N+ + + S
Sbjct: 371 PKLAKTLIPSHAMNQIELPSPLFPNLAISSSNQSSSSNSNANVSSNLNSHSASPSTTSSS 430
Query: 214 SSTPNDDWLQS-RISNPNQGSENNPVQRT 131
SSTPN +QS +N + NN T
Sbjct: 431 SSTPNSIPVQSPSTTNTTSSTTNNTTTTT 459
>sp|Q9V3D6|CPSB_DROME Probable cleavage and polyadenylation specificity factor, 100 kDa
subunit (CPSF 100 kDa subunit)
Length = 756
Score = 32.7 bits (73), Expect = 0.95
Identities = 22/90 (24%), Positives = 40/90 (44%), Gaps = 13/90 (14%)
Frame = -1
Query: 292 KINRSSSVNRNVLIPNNVTQNVEIVSSSTPNDDWLQSRISNPNQGSENNPVQRTDLISSE 113
K S + ++ + NNV+ NV + + +W+ +++ +G+ NNP Q +
Sbjct: 255 KNKESGLMAYSLALLNNVSYNV--IEFAKSQIEWMSDKLTKAFEGARNNPFQFKHIQLCH 312
Query: 112 SKADINEFP-------------EQGITRNL 62
S AD+ + P E G TR+L
Sbjct: 313 SLADVYKLPAGPKVVLASTPDLESGFTRDL 342
>sp|P34545|CBP1_CAEEL Protein cbp-1
Length = 2056
Score = 32.7 bits (73), Expect = 0.95
Identities = 29/116 (25%), Positives = 47/116 (40%), Gaps = 8/116 (6%)
Frame = -1
Query: 466 KKSKFTSQHNS--------VSFQSFDVVRHDDSIEDNYPAPNTAKSLAAKFKGEPPMPIG 311
KKS+ S ++S S ++F D +++N P+T+ + G P P G
Sbjct: 7 KKSRADSDYDSGLDALSALESLEAFPTSSKDTDVDNN---PSTSAGGSGGPSGSTPHPQG 63
Query: 310 SNQSQSKINRSSSVNRNVLIPNNVTQNVEIVSSSTPNDDWLQSRISNPNQGSENNP 143
+ Q N ++ P QN+ N LQ + NP+Q S N+P
Sbjct: 64 TPQPAPSNNGGFNLQPGQSQPQQPPQNM----GGGVNGSVLQELLMNPSQTSNNSP 115
>sp|P0C0R4|LIP_STAES Lipase precursor (Glycerol ester hydrolase)
Length = 688
Score = 32.7 bits (73), Expect = 0.95
Identities = 17/76 (22%), Positives = 40/76 (52%)
Frame = -1
Query: 307 NQSQSKINRSSSVNRNVLIPNNVTQNVEIVSSSTPNDDWLQSRISNPNQGSENNPVQRTD 128
++ QS N++ + NV N++T+N + + + N+DW+Q + + N + V++
Sbjct: 61 SEQQSTQNKNVNEKSNV---NSITENESLHNETPKNEDWIQQQKDSQNDNKSESVVEQ-- 115
Query: 127 LISSESKADINEFPEQ 80
+ E++A + E+
Sbjct: 116 --NKENEAFVQNHSEE 129
>sp|Q7M3S9|RNGB_DICDI Ring finger protein B (Protein rngB)
Length = 943
Score = 32.7 bits (73), Expect = 0.95
Identities = 18/79 (22%), Positives = 40/79 (50%)
Frame = -1
Query: 373 APNTAKSLAAKFKGEPPMPIGSNQSQSKINRSSSVNRNVLIPNNVTQNVEIVSSSTPNDD 194
+P + SLA+ ++ + NQ+Q+ N +++ N N N++ +++T N++
Sbjct: 386 SPLSTSSLASSYQSSQSLQFNQNQNQNNNNNNNNNNNN---------NIQTTTTTTTNNN 436
Query: 193 WLQSRISNPNQGSENNPVQ 137
+ +N N + NN V+
Sbjct: 437 --NNNNNNNNNNNNNNNVE 453
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,842,599
Number of Sequences: 369166
Number of extensions: 1629013
Number of successful extensions: 4674
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4425
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4650
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6073541875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)