Planarian EST Database


Dr_sW_019_H23

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_019_H23
         (731 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P13230|GRP33_ARTSA  Glycine-rich protein GRP33                  78   2e-14
sp|Q60749|SAM68_MOUSE  KH domain containing, RNA binding, si...    66   8e-11
sp|Q07666|SAM68_HUMAN  KH domain containing, RNA binding, si...    66   8e-11
sp|Q91V33|SAM68_RAT  KH domain containing, RNA binding, sign...    66   8e-11
sp|Q8UUW7|SAM68_CHICK  KH domain containing, RNA binding, si...    66   8e-11
sp|O01367|HOW_DROME  Held out wings protein (KH-domain prote...    44   4e-04
sp|Q17339|GLD1_CAEEL  Female germline-specific tumor suppres...    40   0.005
sp|Q8NHZ7|MB3L2_HUMAN  Methyl-CpG binding domain protein 3-l...    33   1.0  
sp|Q10900|CTPI_MYCTU  Probable cation-transporting ATPase I        33   1.0  
sp|P25805|CYSP_PLAFA  Trophozoite cysteine proteinase precur...    30   6.6  
>sp|P13230|GRP33_ARTSA Glycine-rich protein GRP33
          Length = 308

 Score = 78.2 bits (191), Expect = 2e-14
 Identities = 41/88 (46%), Positives = 57/88 (64%), Gaps = 7/88 (7%)
 Frame = +2

Query: 2   GAGSLRNPEKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIP- 178
           G GS+R+  KE+EL NSGD KY HL ++LH++I SI +P+EA+  ++YAL E+KK + P 
Sbjct: 117 GRGSMRDRNKEEELRNSGDVKYAHLNEQLHIEIISIASPAEAHARMAYALTEIKKYITPE 176

Query: 179 -DPN-----EQAPGQMPMVTPLMGGPNP 244
            DPN         G M  +  +MGGP P
Sbjct: 177 EDPNYMMMAGHGAGPMMGMGGMMGGPGP 204
>sp|Q60749|SAM68_MOUSE KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score = 66.2 bits (160), Expect = 8e-11
 Identities = 36/85 (42%), Positives = 51/85 (60%), Gaps = 1/85 (1%)
 Frame = +2

Query: 2   GAGSLRNPEKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD 181
           G GS+R+  KE+EL   GDPKY HL   LH+ I+  G P EAY  +++A+ EVKK ++PD
Sbjct: 199 GKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPD 258

Query: 182 -PNEQAPGQMPMVTPLMGGPNPGFG 253
             ++    Q   ++ L G P P  G
Sbjct: 259 MMDDICQEQFLELSYLNGVPEPSRG 283
>sp|Q07666|SAM68_HUMAN KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score = 66.2 bits (160), Expect = 8e-11
 Identities = 36/85 (42%), Positives = 51/85 (60%), Gaps = 1/85 (1%)
 Frame = +2

Query: 2   GAGSLRNPEKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD 181
           G GS+R+  KE+EL   GDPKY HL   LH+ I+  G P EAY  +++A+ EVKK ++PD
Sbjct: 199 GKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPD 258

Query: 182 -PNEQAPGQMPMVTPLMGGPNPGFG 253
             ++    Q   ++ L G P P  G
Sbjct: 259 MMDDICQEQFLELSYLNGVPEPSRG 283
>sp|Q91V33|SAM68_RAT KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score = 66.2 bits (160), Expect = 8e-11
 Identities = 36/85 (42%), Positives = 51/85 (60%), Gaps = 1/85 (1%)
 Frame = +2

Query: 2   GAGSLRNPEKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD 181
           G GS+R+  KE+EL   GDPKY HL   LH+ I+  G P EAY  +++A+ EVKK ++PD
Sbjct: 199 GKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPD 258

Query: 182 -PNEQAPGQMPMVTPLMGGPNPGFG 253
             ++    Q   ++ L G P P  G
Sbjct: 259 MMDDICQEQFLELSYLNGVPEPSRG 283
>sp|Q8UUW7|SAM68_CHICK KH domain containing, RNA binding, signal transduction associated
           protein 1 (Src-associated in mitosis 68 kDa protein)
           (Sam68)
          Length = 433

 Score = 66.2 bits (160), Expect = 8e-11
 Identities = 39/100 (39%), Positives = 53/100 (53%), Gaps = 10/100 (10%)
 Frame = +2

Query: 2   GAGSLRNPEKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD 181
           G GS+R+  KE+EL   GDPKY HL   LH+ I+  G P EAY  +++A+ EVKK ++PD
Sbjct: 182 GKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPD 241

Query: 182 PNEQAPGQ----------MPMVTPLMGGPNPGFGAMRTQP 271
             +    +          +P  T   GGP  G GA    P
Sbjct: 242 MMDDICQEQFLELSYLNGVPEPTRGRGGPVRGRGAAPPPP 281
>sp|O01367|HOW_DROME Held out wings protein (KH-domain protein KH93F) (Putative
           RNA-binding protein) (Muscle-specific protein) (Wings
           held out protein) (Struthio protein) (Quaking-related
           93F)
          Length = 405

 Score = 43.9 bits (102), Expect = 4e-04
 Identities = 25/68 (36%), Positives = 39/68 (57%)
 Frame = +2

Query: 2   GAGSLRNPEKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD 181
           G GS+R+ +KE    N G P + HL D LH+ I      + A   ++ A+AEV+K+++P 
Sbjct: 180 GKGSMRDKKKEDA--NRGKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVP- 236

Query: 182 PNEQAPGQ 205
              QA G+
Sbjct: 237 ---QAEGE 241
>sp|Q17339|GLD1_CAEEL Female germline-specific tumor suppressor gld-1 (Defective in germ
           line development protein 1)
          Length = 463

 Score = 40.4 bits (93), Expect = 0.005
 Identities = 20/61 (32%), Positives = 33/61 (54%)
 Frame = +2

Query: 2   GAGSLRNPEKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD 181
           G GS+R  +K +E  + G   + HL D LH+ +      +  +  +  AL +VKK++IP 
Sbjct: 248 GKGSMR--DKSKESAHRGKANWEHLEDDLHVLVQCEDTENRVHIKLQAALEQVKKLLIPA 305

Query: 182 P 184
           P
Sbjct: 306 P 306
>sp|Q8NHZ7|MB3L2_HUMAN Methyl-CpG binding domain protein 3-like 2 (MBD3-like 2)
          Length = 204

 Score = 32.7 bits (73), Expect = 1.0
 Identities = 24/67 (35%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
 Frame = +2

Query: 86  LHLQ-IDSIGNPSEAYYNISYALAEVKKVMIPDPNEQAPGQMPMVTPLMGGPNPGFGAMR 262
           LHL+ + SI  P  A  ++  A AE     +  P E  PG+ P V    GGP PG G   
Sbjct: 109 LHLESVLSILAPGTASESLDRAGAE----RVRSPLEPTPGRFPAVA---GGPTPGMGCQL 161

Query: 263 TQPMTQQ 283
             P++ Q
Sbjct: 162 PPPLSGQ 168
>sp|Q10900|CTPI_MYCTU Probable cation-transporting ATPase I
          Length = 1625

 Score = 32.7 bits (73), Expect = 1.0
 Identities = 24/84 (28%), Positives = 36/84 (42%), Gaps = 9/84 (10%)
 Frame = +2

Query: 8   GSLRNPEKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPDPN 187
           G+LR  ++ + ++  G       R  LH Q D  G   +  Y ++ AL   ++   PDP+
Sbjct: 412 GALRRLDRVRVVVIDGAALRGDNRAVLHAQGDEPGWDDDRVYEVADALLHGEQAPEPDPD 471

Query: 188 E---------QAPGQMPMVTPLMG 232
           E          AP Q P  TP  G
Sbjct: 472 ELPATGARLRWAPAQGPSATPAQG 495
>sp|P25805|CYSP_PLAFA Trophozoite cysteine proteinase precursor (TCP)
          Length = 569

 Score = 30.0 bits (66), Expect = 6.6
 Identities = 16/56 (28%), Positives = 28/56 (50%)
 Frame = +2

Query: 26  EKEQELLNSGDPKYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPDPNEQ 193
           E+++E        YN+  +  +++ DSI +  E Y N+   L E  K  I + NE+
Sbjct: 103 EEDEEKYTLNSETYNNKNNVSNIKNDSIKSKKEEYINLERILLEKYKKFINENNEE 158
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,469,059
Number of Sequences: 369166
Number of extensions: 1441451
Number of successful extensions: 3230
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3225
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6534486000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)