Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_H01
(935 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6GYQ0|GRIPE_HUMAN GTPase-activating Rap/Ran-GAP domain-... 292 1e-78
sp|Q6GYP7|GRIPE_MOUSE GTPase-activating RapGAP domain-like ... 288 2e-77
sp|O55007|GRIPE_RAT GTPase-activating RapGAP domain-like 1 ... 150 6e-36
sp|P49815|TSC2_HUMAN Tuberin (Tuberous sclerosis 2 protein) 102 1e-21
sp|P49816|TSC2_RAT Tuberin (Tuberous sclerosis 2 homolog pr... 97 9e-20
sp|Q61037|TSC2_MOUSE Tuberin (Tuberous sclerosis 2 homolog ... 93 1e-18
sp|Q9UUG9|TSC2_SCHPO Tuberous sclerosis 2 protein homolog 81 4e-15
sp|P47736|RGP2_HUMAN Rap1 GTPase-activating protein 1 (Rap1... 72 2e-12
sp|Q80TE4|SI1L2_MOUSE Signal-induced proliferation-associat... 63 1e-09
sp|Q9P2F8|SI1L2_HUMAN Signal-induced proliferation-associat... 63 1e-09
>sp|Q6GYQ0|GRIPE_HUMAN GTPase-activating Rap/Ran-GAP domain-like 1 (GAP-related-interacting
partner to E12) (GRIPE) (Tuberin-like protein 1)
Length = 2036
Score = 292 bits (747), Expect = 1e-78
Identities = 143/267 (53%), Positives = 188/267 (70%), Gaps = 1/267 (0%)
Frame = +2
Query: 8 PNSKFYFCRQILTQLGFLSWKRRASIDLIQKTN-ILRHLKFIDQRRGRETHKIAVLYVAW 184
P S FY+CR +L+ LG SW +R S L++K +LR L+ +D R+ RETHKIAV YVA
Sbjct: 1758 PQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAE 1817
Query: 185 GQEDKESILGNTTGSLEFNTFINGLGWKVSLENHKGFMGGLESSGRNGCFAPYFANSTLE 364
GQEDK SIL NT GS + F+ GLGW+V+L NH GFMGGL+ + G PYFA ST+E
Sbjct: 1818 GQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVE 1877
Query: 365 VMFHVSTWMPSEKPEDKQNKFKHLGNDEVMVIWSENYRDYRSHITKTEFGDVMIIIHPLR 544
V+FHVST MPS+ + K +HLGNDEV ++WSE+ RDYR I TEFGDV+I+I+P++
Sbjct: 1878 VIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMK 1937
Query: 545 NTLFGINIKTKSDVWTKFFGPLFDGAIVGEKELPVLVRATAVNAGRKIRECKQLYEEFFV 724
N +F I I K +V FFGPLFDGAIV K LP++VRATA+NA R ++ LY+ F+
Sbjct: 1938 NHMFSIQIMKKPEV--PFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYE 1995
Query: 725 ERAKYLEKAIKDFCEPMSFEDFAEHIY 805
ERA+YL+ ++ EP +FEDFA ++
Sbjct: 1996 ERARYLQTIVQHHLEPTTFEDFAAQVF 2022
>sp|Q6GYP7|GRIPE_MOUSE GTPase-activating RapGAP domain-like 1 (GAP-related-interacting
partner to E12) (GRIPE) (Tuberin-like protein 1)
Length = 2035
Score = 288 bits (736), Expect = 2e-77
Identities = 141/267 (52%), Positives = 187/267 (70%), Gaps = 1/267 (0%)
Frame = +2
Query: 8 PNSKFYFCRQILTQLGFLSWKRRASIDLIQKTN-ILRHLKFIDQRRGRETHKIAVLYVAW 184
P S FY+CR +L+ LG SW +R S L++K +LR L+ +D R+ RETHKIAV YVA
Sbjct: 1757 PQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAE 1816
Query: 185 GQEDKESILGNTTGSLEFNTFINGLGWKVSLENHKGFMGGLESSGRNGCFAPYFANSTLE 364
GQEDK SIL N GS + F+ GLGW+V+L NH GFMGGL+ + G PYFA ST+E
Sbjct: 1817 GQEDKYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNRSTGLTTPYFATSTVE 1876
Query: 365 VMFHVSTWMPSEKPEDKQNKFKHLGNDEVMVIWSENYRDYRSHITKTEFGDVMIIIHPLR 544
V+FHVST MPS+ + K +HLGNDEV ++WSE+ RDYR I TEFGDV+I+I+P++
Sbjct: 1877 VIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMK 1936
Query: 545 NTLFGINIKTKSDVWTKFFGPLFDGAIVGEKELPVLVRATAVNAGRKIRECKQLYEEFFV 724
N +F I I K +V FFGPLFDGAIV K LP++VR+TA+NA R ++ LY+ F+
Sbjct: 1937 NHMFSIQIMKKPEV--PFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYE 1994
Query: 725 ERAKYLEKAIKDFCEPMSFEDFAEHIY 805
ERA+YL+ ++ EP +FEDFA ++
Sbjct: 1995 ERARYLQTIVQHHLEPTTFEDFAAQVF 2021
>sp|O55007|GRIPE_RAT GTPase-activating RapGAP domain-like 1 (GAP-related-interacting
partner to E12) (GRIPE) (Tuberin-like protein 1)
Length = 747
Score = 150 bits (378), Expect = 6e-36
Identities = 76/140 (54%), Positives = 95/140 (67%), Gaps = 1/140 (0%)
Frame = +2
Query: 8 PNSKFYFCRQILTQLGFLSWKRRASIDLIQKTN-ILRHLKFIDQRRGRETHKIAVLYVAW 184
P S FY+CR +L+ LG SW +R S L++K +LR L+ +D R+ RETHKIAV YVA
Sbjct: 583 PQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAE 642
Query: 185 GQEDKESILGNTTGSLEFNTFINGLGWKVSLENHKGFMGGLESSGRNGCFAPYFANSTLE 364
GQEDK SIL N GS + F+ GLGW+V+L NH GFMGGL+ + G PYFA ST+E
Sbjct: 643 GQEDKYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVE 702
Query: 365 VMFHVSTWMPSEKPEDKQNK 424
V+FHVST MPSE + K
Sbjct: 703 VIFHVSTRMPSESDDSLTKK 722
>sp|P49815|TSC2_HUMAN Tuberin (Tuberous sclerosis 2 protein)
Length = 1807
Score = 102 bits (255), Expect = 1e-21
Identities = 66/237 (27%), Positives = 127/237 (53%), Gaps = 12/237 (5%)
Frame = +2
Query: 113 RHLKFIDQRRGRETHKIAVLYVAWGQEDKE-SILGNTTGSLEFNTFINGLGWKVSLEN-- 283
R ++ +DQ +THKIAVLYV GQ + E +IL N GS + F+ GLG + L++
Sbjct: 1529 RSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQ 1588
Query: 284 -HKGFMGGLESSGRNGCFAPYFANSTLEVMFHVSTWMPSEKPEDKQ--NKFKHLGNDEVM 454
K ++GGL+ G +G F + + ++ +FH++T MP+ K DK +K +HLGND V
Sbjct: 1589 PDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPT-KDVDKHRCDKKRHLGNDFVS 1647
Query: 455 VIWSENYRDYRSHITKTEFGDVMIIIHPLRNTLFGINIKTKSDVWTKFFGPLFDGAIVGE 634
++++++ D++ K +F V +I+ PL ++++ + D+ + IV +
Sbjct: 1648 IVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDM--EGLVDTSVAKIVSD 1705
Query: 635 KELPVLVRATAVNAGRKIR------ECKQLYEEFFVERAKYLEKAIKDFCEPMSFED 787
+ LP + R A++A + +Y ++ R +++++ + CE ++ +
Sbjct: 1706 RNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSN 1762
>sp|P49816|TSC2_RAT Tuberin (Tuberous sclerosis 2 homolog protein)
Length = 1809
Score = 96.7 bits (239), Expect = 9e-20
Identities = 64/202 (31%), Positives = 110/202 (54%), Gaps = 6/202 (2%)
Frame = +2
Query: 89 LIQKTNILRHLKFIDQRRGRETHKIAVLYVAWGQEDKE-SILGNTTGSLEFNTFINGLGW 265
L+ + R ++ +DQ +THKIAVLYV GQ E +IL N GS + F+ GLG
Sbjct: 1523 LLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGR 1582
Query: 266 KVSLEN---HKGFMGGLESSGRNGCFAPYFANSTLEVMFHVSTWMPSEKPEDKQ--NKFK 430
+ L++ K ++GGL+ G +G F + + ++ +FH++T MP+ K DK +K +
Sbjct: 1583 LIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPT-KDVDKHRCDKKR 1641
Query: 431 HLGNDEVMVIWSENYRDYRSHITKTEFGDVMIIIHPLRNTLFGINIKTKSDVWTKFFGPL 610
HLGND V +I++++ D++ K +F V +II PL + ++ + D+ +
Sbjct: 1642 HLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDM--EGLVDT 1699
Query: 611 FDGAIVGEKELPVLVRATAVNA 676
IV ++ L + R A++A
Sbjct: 1700 SVAKIVSDRNLSFVARQMALHA 1721
>sp|Q61037|TSC2_MOUSE Tuberin (Tuberous sclerosis 2 homolog protein)
Length = 1814
Score = 92.8 bits (229), Expect = 1e-18
Identities = 66/237 (27%), Positives = 122/237 (51%), Gaps = 17/237 (7%)
Frame = +2
Query: 89 LIQKTNILRHLKFIDQRRGRETHKIAVLYVAWGQEDKE-SILGNTTGSLEFNTFINGLGW 265
L+ + R ++ +DQ +THKIAVLYV GQ E +IL N GS + F+ GLG
Sbjct: 1522 LLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGR 1581
Query: 266 KVSLEN---HKGFMGGLESSGRNGCFAPYFANSTLEVMFHVSTWMPSEKPEDKQ--NKFK 430
+ L++ K ++GGL+ G +G F + + ++ +FH++T MP+ K DK +K +
Sbjct: 1582 LIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPT-KDVDKHRCDKKR 1640
Query: 431 HLGNDEVMVIWSENYRDYR-SHITKTEFGDVMIIIHPLRNTLFGINIKTKSD------VW 589
HLGND V +I++++ D++ I + +F V +II PL + ++ + D W
Sbjct: 1641 HLGNDFVSIIYNDSGEDFKLGTIKQGQFNFVHVIITPLDYKCNLLTLQCRKDGPACKCEW 1700
Query: 590 TKFFGPLFDGAIVGEKELPVLVRATAVNAGRKIRECK----QLYEEFFVERAKYLEK 748
+ G + A+ EL V + + ++ + +Y ++ R +++++
Sbjct: 1701 WRQPGEIVVWALPVVMELTVTILLCHLQMASQVHHSRSNPTDIYPSKWIARLRHIKR 1757
>sp|Q9UUG9|TSC2_SCHPO Tuberous sclerosis 2 protein homolog
Length = 1339
Score = 81.3 bits (199), Expect = 4e-15
Identities = 69/245 (28%), Positives = 115/245 (46%), Gaps = 16/245 (6%)
Frame = +2
Query: 104 NILRHLKFIDQRRGRETHKIAVLYVAWGQEDKESILGNTTGSLEFNTFINGLGWKVSLE- 280
++ R + D+ E+ K ++YV + Q + IL NT S +F TF+NGLG L+
Sbjct: 1075 SVRRAISVFDRIPVIESLKAGLVYVGYQQRREADILANTNPSEDFLTFLNGLGTLFELKT 1134
Query: 281 NHKGFMGGLE-SSGRNGCFAPYFANSTLEVMFHVSTWMPSEKPEDK--QNKFKHLGNDEV 451
+ K F GGL+ + +G FA + + +++FH +T MP+ D K +H+GND V
Sbjct: 1135 DQKVFAGGLDRENDIDGAFAYCWKDKVTQMVFHCTTMMPTNIEHDPGCTLKKRHIGNDFV 1194
Query: 452 MVIWSENYRDYRSHITKTEFGDVMIIIHP----LRNT-----LFGINIKTKSDVWTKFFG 604
+I++E+ +Y ++F V I+I P +R T + + TK D+ F
Sbjct: 1195 TIIFNESGLEYDFDTIPSQFNFVNIVITPESESIRRTGRQIKFYKVKALTKYDIDFSLFR 1254
Query: 605 PLFDGAIVGEKELPVLVRATAVNA---GRKIRECKQLYEEFFVERAKYLEKAIKDFCEPM 775
IV LP +VR +NA Y + ER + L++ + F +
Sbjct: 1255 RY---KIVSSDALPAIVRDVTLNAAVFSHIYHRSAGDYVHIWAERLRQLKRLREKFQASV 1311
Query: 776 SFEDF 790
ED+
Sbjct: 1312 LPEDY 1316
>sp|P47736|RGP2_HUMAN Rap1 GTPase-activating protein 1 (Rap1GAP)
Length = 663
Score = 72.0 bits (175), Expect = 2e-12
Identities = 46/156 (29%), Positives = 78/156 (50%), Gaps = 6/156 (3%)
Frame = +2
Query: 158 KIAVLYVAWGQEDKESILGNTTGSLEFNTFINGLGWKVSLENHKGFMGGLE-SSGRNGCF 334
K V+Y GQ +E + S F F+ LG KV L++ KGF GGL+ + G+ G
Sbjct: 194 KFGVIYQKLGQTSEEELFSTNEESPAFVEFLEFLGQKVKLQDFKGFRGGLDVTHGQTGTE 253
Query: 335 APYFANSTLEVMFHVSTWMPSEKPEDKQ-NKFKHLGNDEVMVIWSENYRDYRSHITKTEF 511
+ Y E+MFHVST +P + + +Q + +H+GND V V++ + + + + F
Sbjct: 254 SVYCNFRNKEIMFHVSTKLPYTEGDAQQLQRKRHIGNDIVAVVFQDENTPFVPDMIASNF 313
Query: 512 GDVMIIIHP----LRNTLFGINIKTKSDVWTKFFGP 607
+++ L+ +++ + DV FFGP
Sbjct: 314 LHAYVVVQAEGGGPDGPLYKVSVTARDDV--PFFGP 347
>sp|Q80TE4|SI1L2_MOUSE Signal-induced proliferation-associated 1-like protein 2
Length = 1185
Score = 63.2 bits (152), Expect = 1e-09
Identities = 55/218 (25%), Positives = 94/218 (43%), Gaps = 15/218 (6%)
Frame = +2
Query: 155 HKIAVLYVAWGQEDKESILGNTTGSLEFNTFINGLGWKVSLENHKGFMGGLES-SGRNGC 331
HKI +LY GQ +E + N T F F++ LG +V L+ + L++ + G
Sbjct: 71 HKIGILYCRAGQSTEEEMYNNETAGPAFEEFLDLLGQRVRLKGFSKYRAQLDNKTDSTGT 130
Query: 332 FAPYFANSTLEVMFHVSTWMPSEKPEDKQN--KFKHLGNDEVMVIWSE------NYRDYR 487
+ Y E+MFHVST +P P ++Q + +H+GND V +++ E ++ R
Sbjct: 131 HSLYTTYKDFELMFHVSTLLP-YMPNNRQQLLRKRHIGNDIVTIVFQEPGALPFTPKNIR 189
Query: 488 SHITKTEFGDVMIIIHP----LRNTLFGINIKTKSDVWTKFFGPLFDGAIVGEKELPV-- 649
SH F V +I+ N + + + DV FGP + K
Sbjct: 190 SH-----FQHVFVIVKVHNPCTENVCYSVGVSRSKDV--PPFGPPIPKGVTFPKSAVFRD 242
Query: 650 LVRATAVNAGRKIRECKQLYEEFFVERAKYLEKAIKDF 763
+ A +NA + ++ R +YL+ ++F
Sbjct: 243 FLLAKVINAENAAHKSEKFRAMATRTRQEYLKDLAENF 280
>sp|Q9P2F8|SI1L2_HUMAN Signal-induced proliferation-associated 1-like protein 2
Length = 1514
Score = 62.8 bits (151), Expect = 1e-09
Identities = 52/213 (24%), Positives = 92/213 (43%), Gaps = 10/213 (4%)
Frame = +2
Query: 155 HKIAVLYVAWGQEDKESILGNTTGSLEFNTFINGLGWKVSLENHKGFMGGLES-SGRNGC 331
HKI +LY GQ +E + N T F F++ LG +V L+ + L++ + G
Sbjct: 399 HKIGILYCKAGQSTEEEMYNNETAGPAFEEFLDLLGQRVRLKGFSKYRAQLDNKTDSTGT 458
Query: 332 FAPYFANSTLEVMFHVSTWMPSEKPEDKQN--KFKHLGNDEVMVIWSE-NYRDYRSHITK 502
+ Y E+MFHVST +P P ++Q + +H+GND V +++ E + +
Sbjct: 459 HSLYTTYKDYELMFHVSTLLP-YMPNNRQQLLRKRHIGNDIVTIVFQEPGALPFTPKSIR 517
Query: 503 TEFGDVMIIIHP----LRNTLFGINIKTKSDVWTKFFGPLFDGAIVGEKELPV--LVRAT 664
+ F V +I+ N + + + DV FGP + K + A
Sbjct: 518 SHFQHVFVIVKVHNPCTENVCYSVGVSRSKDV--PPFGPPIPKGVTFPKSAVFRDFLLAK 575
Query: 665 AVNAGRKIRECKQLYEEFFVERAKYLEKAIKDF 763
+NA + ++ R +YL+ ++F
Sbjct: 576 VINAENAAHKSEKFRAMATRTRQEYLKDLAENF 608
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 110,906,136
Number of Sequences: 369166
Number of extensions: 2308129
Number of successful extensions: 6013
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5739
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5985
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9654860130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)