Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_G11
(927 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P42356|PI4KA_HUMAN Phosphatidylinositol 4-kinase alpha (... 275 1e-73
sp|P37297|STT4_YEAST Phosphatidylinositol 4-kinase STT4 (PI... 224 3e-58
sp|Q9UBF8|PI4KB_HUMAN Phosphatidylinositol 4-kinase beta (P... 163 6e-40
sp|O08561|PI4KB_RAT Phosphatidylinositol 4-kinase beta (Ptd... 161 2e-39
sp|Q8BKC8|PI4KB_MOUSE Phosphatidylinositol 4-kinase beta (P... 161 2e-39
sp|P54677|PI4K_DICDI Phosphatidylinositol 4-kinase (PI4-kin... 160 4e-39
sp|O02810|PI4KB_BOVIN Phosphatidylinositol 4-kinase beta (P... 160 4e-39
sp|Q9UW24|PIK1A_CANAL Phosphatidylinositol 4-kinase PIK1a (... 140 7e-33
sp|P39104|PIK1_YEAST Phosphatidylinositol 4-kinase PIK1 (PI... 136 7e-32
sp|Q9UW20|PIK1_CANAL Phosphatidylinositol 4-kinase PIK1alph... 134 4e-31
>sp|P42356|PI4KA_HUMAN Phosphatidylinositol 4-kinase alpha (PI4-kinase) (PtdIns-4-kinase)
(PI4K-alpha)
Length = 2044
Score = 275 bits (703), Expect = 1e-73
Identities = 125/188 (66%), Positives = 159/188 (84%)
Frame = +1
Query: 364 IYYQACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYRVVATSPGCGVIECVPD 543
I +QA IFKVGDD RQD+LALQ+I LFKNI + GLD +++PYRVVAT+PGCGVIEC+PD
Sbjct: 1784 ISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPD 1843
Query: 544 SKSRDQIGRTTDSGMYDYFLSLFGEETTPSYQEARRNFVMSMASYSVVCYLLQIKDRHNG 723
SRDQ+GR TD GMYDYF +G+E+T ++Q+AR NF+ SMA+YS++ +LLQIKDRHNG
Sbjct: 1844 CTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNG 1903
Query: 724 NIMIDKHGHIIHIDFGFMFESSPGNNMGFEPDIKLTKEMMMVMGGKLDSPAFQWIEQLSV 903
NIM+DK GHIIHIDFGFMFESSPG N+G+EPDIKLT EM+M+MGGK+++ F+W ++ V
Sbjct: 1904 NIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCV 1963
Query: 904 QAYLVLRP 927
+ YL +RP
Sbjct: 1964 RGYLAVRP 1971
Score = 130 bits (328), Expect = 4e-30
Identities = 60/84 (71%), Positives = 74/84 (88%), Gaps = 3/84 (3%)
Frame = +3
Query: 3 TNVSGVIRPYPKGQQRQQACLEALRKIELQPGCYLPSNPDAIVLEIDYLSGIPLQSAAKA 182
TNVS +I+PYPKG +R++ACL AL ++++QPGCYLPSNP+AIVL+IDY SG P+QSAAKA
Sbjct: 1684 TNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKA 1743
Query: 183 PYLAKFKVKRCGVQKLEK---KCR 245
PYLAKFKVKRCGV +LEK +CR
Sbjct: 1744 PYLAKFKVKRCGVSELEKEGLRCR 1767
>sp|P37297|STT4_YEAST Phosphatidylinositol 4-kinase STT4 (PI4-kinase) (PtdIns-4-kinase)
Length = 1900
Score = 224 bits (570), Expect = 3e-58
Identities = 114/208 (54%), Positives = 147/208 (70%), Gaps = 2/208 (0%)
Frame = +1
Query: 310 THEIKENRKNNPID--NKDDIYYQACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYL 483
T +IK++ K+ P+ NK+ +QA IFKVGDD RQD+LALQ+I LF+ I GLD Y+
Sbjct: 1620 TFKIKKDVKD-PLTGKNKEVEKWQAAIFKVGDDCRQDVLALQLISLFRTIWSSIGLDVYV 1678
Query: 484 YPYRVVATSPGCGVIECVPDSKSRDQIGRTTDSGMYDYFLSLFGEETTPSYQEARRNFVM 663
+PYRV AT+PGCGVI+ +P+S SRD +GR +G+Y+YF S FG E+T +Q AR NFV
Sbjct: 1679 FPYRVTATAPGCGVIDVLPNSVSRDMLGREAVNGLYEYFTSKFGNESTIEFQNARNNFVK 1738
Query: 664 SMASYSVVCYLLQIKDRHNGNIMIDKHGHIIHIDFGFMFESSPGNNMGFEPDIKLTKEMM 843
S+A YSV+ YLLQ KDRHNGNIM D GH +HIDFGF+F+ PG KLTKEM+
Sbjct: 1739 SLAGYSVISYLLQFKDRHNGNIMYDDQGHCLHIDFGFIFDIVPGGIKFEAVPFKLTKEMV 1798
Query: 844 MVMGGKLDSPAFQWIEQLSVQAYLVLRP 927
VMGG +PA+ E+L ++AYL RP
Sbjct: 1799 KVMGGSPQTPAYLDFEELCIKAYLAARP 1826
Score = 73.9 bits (180), Expect = 6e-13
Identities = 38/85 (44%), Positives = 56/85 (65%), Gaps = 2/85 (2%)
Frame = +3
Query: 3 TNVSGVIRPYPKGQ--QRQQACLEALRKIELQPGCYLPSNPDAIVLEIDYLSGIPLQSAA 176
T +SG ++PY K +++ E + KIE++P YLPSNPD +V++ID SG PLQS A
Sbjct: 1554 TGISGKLKPYIKKSKAEKKHKIDEEMSKIEVKPDVYLPSNPDGVVIDIDRKSGKPLQSHA 1613
Query: 177 KAPYLAKFKVKRCGVQKLEKKCRDV 251
KAP++A FK+K+ L K ++V
Sbjct: 1614 KAPFMATFKIKKDVKDPLTGKNKEV 1638
>sp|Q9UBF8|PI4KB_HUMAN Phosphatidylinositol 4-kinase beta (PtdIns 4-kinase) (PI4Kbeta)
(PI4K-beta) (NPIK) (PI4K92)
Length = 816
Score = 163 bits (413), Expect = 6e-40
Identities = 86/204 (42%), Positives = 129/204 (63%), Gaps = 1/204 (0%)
Frame = +1
Query: 316 EIKENRKNNPIDNKDDIYYQACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYR 495
+++ R+ +P + + + I K GDD+RQ++LA QV++ ++I E+E + ++ PY+
Sbjct: 540 KVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYK 599
Query: 496 VVATSPGCGVIECVPDSKSRDQIGRTTDSGMYDYFLSLFGEETTPSYQEARRNFVMSMAS 675
++ S G+IE V ++ S Q+ + + + DYFL G TT ++ A+RNFV S A
Sbjct: 600 ILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAG 659
Query: 676 YSVVCYLLQIKDRHNGNIMIDKHGHIIHIDFGFMFESSPGNNMGFEPD-IKLTKEMMMVM 852
Y +VCYLLQ+KDRHNGNI++D GHIIHIDFGF+ SSP N+GFE KLT E + VM
Sbjct: 660 YCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVM 718
Query: 853 GGKLDSPAFQWIEQLSVQAYLVLR 924
GG LD F + + L +Q + R
Sbjct: 719 GG-LDGDMFNYYKMLMLQGLIAAR 741
>sp|O08561|PI4KB_RAT Phosphatidylinositol 4-kinase beta (PtdIns 4-kinase) (PI4Kbeta)
(PI4K-beta)
Length = 816
Score = 161 bits (408), Expect = 2e-39
Identities = 85/204 (41%), Positives = 129/204 (63%), Gaps = 1/204 (0%)
Frame = +1
Query: 316 EIKENRKNNPIDNKDDIYYQACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYR 495
+++ R+ +P + + + I K GDD+RQ++LA QV++ ++I E+E + ++ PY+
Sbjct: 540 KVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYK 599
Query: 496 VVATSPGCGVIECVPDSKSRDQIGRTTDSGMYDYFLSLFGEETTPSYQEARRNFVMSMAS 675
++ S G+IE V ++ S Q+ + + + DYFL G TT ++ A+RNFV S A
Sbjct: 600 ILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAG 659
Query: 676 YSVVCYLLQIKDRHNGNIMIDKHGHIIHIDFGFMFESSPGNNMGFEPD-IKLTKEMMMVM 852
Y +VCYLLQ+KDRHNGNI++D GHIIHIDFGF+ SSP N+GFE KLT E + VM
Sbjct: 660 YCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVM 718
Query: 853 GGKLDSPAFQWIEQLSVQAYLVLR 924
GG L+ F + + L +Q + R
Sbjct: 719 GG-LNGDMFNYYKMLMLQGLIAAR 741
>sp|Q8BKC8|PI4KB_MOUSE Phosphatidylinositol 4-kinase beta (PtdIns 4-kinase) (PI4Kbeta)
(PI4K-beta)
Length = 801
Score = 161 bits (408), Expect = 2e-39
Identities = 85/204 (41%), Positives = 129/204 (63%), Gaps = 1/204 (0%)
Frame = +1
Query: 316 EIKENRKNNPIDNKDDIYYQACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYR 495
+++ R+ +P + + + I K GDD+RQ++LA QV++ ++I E+E + ++ PY+
Sbjct: 525 KVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYK 584
Query: 496 VVATSPGCGVIECVPDSKSRDQIGRTTDSGMYDYFLSLFGEETTPSYQEARRNFVMSMAS 675
++ S G+IE V ++ S Q+ + + + DYFL G TT ++ A+RNFV S A
Sbjct: 585 ILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAG 644
Query: 676 YSVVCYLLQIKDRHNGNIMIDKHGHIIHIDFGFMFESSPGNNMGFEPD-IKLTKEMMMVM 852
Y +VCYLLQ+KDRHNGNI++D GHIIHIDFGF+ SSP N+GFE KLT E + VM
Sbjct: 645 YCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVM 703
Query: 853 GGKLDSPAFQWIEQLSVQAYLVLR 924
GG L+ F + + L +Q + R
Sbjct: 704 GG-LNGDMFNYYKMLMLQGLIAAR 726
>sp|P54677|PI4K_DICDI Phosphatidylinositol 4-kinase (PI4-kinase) (PtdIns-4-kinase)
(PI4K-alpha)
Length = 1093
Score = 160 bits (406), Expect = 4e-39
Identities = 85/205 (41%), Positives = 130/205 (63%), Gaps = 2/205 (0%)
Frame = +1
Query: 316 EIKENRKNNPIDNKDDIYYQACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYR 495
+I+ +K +P + + + I K GDD RQ+ +A+Q+I F I + L YL PY
Sbjct: 813 KIERYKKISPFGDYPNWRLYSVIVKTGDDCRQEQMAVQLISKFDEIWKETRLPLYLRPYS 872
Query: 496 VVATSPGCGVIECVPDSKSRDQIGRTTD--SGMYDYFLSLFGEETTPSYQEARRNFVMSM 669
++ TS G G+IE +PD+ S + ++T + + +YF S +G+ + ++ A+ NF+ SM
Sbjct: 873 ILVTSSGGGIIETIPDTMSLHNLKKSTPGFTTLLNYFKSTYGDPSGLRFRTAQSNFIESM 932
Query: 670 ASYSVVCYLLQIKDRHNGNIMIDKHGHIIHIDFGFMFESSPGNNMGFEPDIKLTKEMMMV 849
A+YS+V Y+LQIKDRHNGNI+IDK GHI+HIDFGF+ +SPGN KLT+E++ V
Sbjct: 933 AAYSIVTYILQIKDRHNGNILIDKEGHIVHIDFGFILSNSPGNISFESAPFKLTQELVDV 992
Query: 850 MGGKLDSPAFQWIEQLSVQAYLVLR 924
MGG + S FQ+ + L V+ + R
Sbjct: 993 MGG-IQSGQFQYFKVLCVRGLIEAR 1016
>sp|O02810|PI4KB_BOVIN Phosphatidylinositol 4-kinase beta (PtdIns 4-kinase) (PI4Kbeta)
(PI4K-beta)
Length = 816
Score = 160 bits (406), Expect = 4e-39
Identities = 85/204 (41%), Positives = 128/204 (62%), Gaps = 1/204 (0%)
Frame = +1
Query: 316 EIKENRKNNPIDNKDDIYYQACIFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYR 495
+++ R+ +P + + + I K GDD+RQ++LA QV++ ++I E+E + ++ PY+
Sbjct: 540 KVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYK 599
Query: 496 VVATSPGCGVIECVPDSKSRDQIGRTTDSGMYDYFLSLFGEETTPSYQEARRNFVMSMAS 675
++ S G+IE V ++ S Q+ + + + DYFL G TT ++ A+RNFV S A
Sbjct: 600 ILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAG 659
Query: 676 YSVVCYLLQIKDRHNGNIMIDKHGHIIHIDFGFMFESSPGNNMGFEPD-IKLTKEMMMVM 852
Y + CYLLQ+KDRHNGNI++D GHIIHIDFGF+ SSP N+GFE KLT E + VM
Sbjct: 660 YCLGCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSP-RNLGFETSAFKLTTEFVDVM 718
Query: 853 GGKLDSPAFQWIEQLSVQAYLVLR 924
GG LD F + + L +Q + R
Sbjct: 719 GG-LDGDMFNYYKMLMLQGLIAAR 741
>sp|Q9UW24|PIK1A_CANAL Phosphatidylinositol 4-kinase PIK1a (PI4-kinase) (PtdIns-4-kinase)
Length = 956
Score = 140 bits (352), Expect = 7e-33
Identities = 80/212 (37%), Positives = 128/212 (60%), Gaps = 15/212 (7%)
Frame = +1
Query: 289 ANNFKKQTHEIKENRKNNPIDNKDDIYYQACIFKVGDDVRQDILALQVIRLFKNILEREG 468
A ++ ++ H I+++ ++N D + I K GDD+ Q+ A Q+I L NI ++
Sbjct: 657 AEDWNEKKHRIRKSSIYGHLENWD---LCSVIVKNGDDLPQEAFACQLISLISNIWKKHK 713
Query: 469 LDHYLYPYRVVATSPGCGVIECVPDSKSRDQIGRTT-----DSG---------MYDYFLS 606
+D + +++ TS G++E + ++ S I ++ ++G + DYF
Sbjct: 714 VDFWTKRMKILITSANAGLVETITNAMSIHSIKKSLTELSIENGENAKGRIFTLKDYFQK 773
Query: 607 LFGEETTPSYQEARRNFVMSMASYSVVCYLLQIKDRHNGNIMIDKHGHIIHIDFGFMFES 786
L+G + Y +A+ NF +S+ASYS++CY+LQIKDRHNGNIM+D GHIIHIDFGF+ +
Sbjct: 774 LYGSVDSSKYVKAQENFAISLASYSIICYVLQIKDRHNGNIMLDHEGHIIHIDFGFLLSN 833
Query: 787 SPGNNMGFE-PDIKLTKEMMMVMGGKLDSPAF 879
SPG ++GFE KLT E + V+GG L+S A+
Sbjct: 834 SPG-SVGFEAAPFKLTSEYVEVLGG-LESKAY 863
>sp|P39104|PIK1_YEAST Phosphatidylinositol 4-kinase PIK1 (PI4-kinase) (PtdIns-4-kinase)
Length = 1066
Score = 136 bits (343), Expect = 7e-32
Identities = 77/197 (39%), Positives = 115/197 (58%), Gaps = 16/197 (8%)
Frame = +1
Query: 382 IFKVGDDVRQDILALQVIRLFKNILEREGLDHYLYPYRVVATSPGCGVIECVPDSKSRDQ 561
I K GDD+RQ+ A Q+I+ NI +E +D ++ +++ TS G++E + ++ S
Sbjct: 794 IAKTGDDLRQEAFAYQMIQAMANIWVKEKVDVWVKRMKILITSANTGLVETITNAMSVHS 853
Query: 562 IGRTTDSGMY---------------DYFLSLFGEETTPSYQEARRNFVMSMASYSVVCYL 696
I + M D+FL FG Y+ A+ NF S+A+YSV+CYL
Sbjct: 854 IKKALTKKMIEDAELDDKGGIASLNDHFLRAFGNPNGFKYRRAQDNFASSLAAYSVICYL 913
Query: 697 LQIKDRHNGNIMIDKHGHIIHIDFGFMFESSPGNNMGFE-PDIKLTKEMMMVMGGKLDSP 873
LQ+KDRHNGNIMID GH+ HIDFGFM +SPG ++GFE KLT E + ++GG ++
Sbjct: 914 LQVKDRHNGNIMIDNEGHVSHIDFGFMLSNSPG-SVGFEAAPFKLTYEYIELLGG-VEGE 971
Query: 874 AFQWIEQLSVQAYLVLR 924
AF+ +L+ ++ LR
Sbjct: 972 AFKKFVELTKSSFKALR 988
>sp|Q9UW20|PIK1_CANAL Phosphatidylinositol 4-kinase PIK1alpha (PI4-kinase)
(PtdIns-4-kinase)
Length = 977
Score = 134 bits (337), Expect = 4e-31
Identities = 84/225 (37%), Positives = 129/225 (57%), Gaps = 15/225 (6%)
Frame = +1
Query: 295 NFKKQTHEIKENRKNNPIDNKDDIYYQACIFKVGDDVRQDILALQVIRLFKNILEREGLD 474
N KKQ IK++ + N D + I K GDD+ Q+ A Q+I + NI ++ +
Sbjct: 682 NTKKQ--RIKKSSAYGHLKNWD---LCSVIAKNGDDLPQEAFACQLISMISNIWKKNNIP 736
Query: 475 HYLYPYRVVATSPGCGVIECVPDSKSRDQIGRT-----TDSG---------MYDYFLSLF 612
+ +++ TS G++E + ++ S I ++ SG + DYF S+F
Sbjct: 737 VWTKRMKILITSANTGLVETITNAMSIHSIKKSFTEHSIKSGENSKGKIFTLLDYFHSVF 796
Query: 613 GEETTPSYQEARRNFVMSMASYSVVCYLLQIKDRHNGNIMIDKHGHIIHIDFGFMFESSP 792
G + S++ A++NF S+A+YS++CY+LQIKDRHNGNIM+D GHIIHIDFGF+ +SP
Sbjct: 797 GSPNSTSFRTAQQNFAKSLAAYSIICYVLQIKDRHNGNIMVDGDGHIIHIDFGFLLSNSP 856
Query: 793 GNNMGFE-PDIKLTKEMMMVMGGKLDSPAFQWIEQLSVQAYLVLR 924
G ++GFE KLT E + ++GG +DS + L Q + LR
Sbjct: 857 G-SVGFEAAPFKLTVEYVELLGG-VDSEIYSQFVYLCKQCFKSLR 899
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,681,735
Number of Sequences: 369166
Number of extensions: 2038865
Number of successful extensions: 5696
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5480
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5652
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9510757740
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)