Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_F07
(547 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial pre... 84 2e-16
sp|Q8BH95|ECHM_MOUSE Enoyl-CoA hydratase, mitochondrial pre... 83 4e-16
sp|Q5R646|ECHM_PONPY Enoyl-CoA hydratase, mitochondrial pre... 83 4e-16
sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precu... 82 8e-16
sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial pre... 82 8e-16
sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Croto... 79 9e-15
sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochon... 74 2e-13
sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF 70 4e-12
sp|Q8Z9L5|CAID_SALTI Carnitinyl-CoA dehydratase (Crotonobet... 70 4e-12
sp|Q8ZRX5|CAID_SALTY Carnitinyl-CoA dehydratase (Crotonobet... 69 6e-12
>sp|P30084|ECHM_HUMAN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 84.3 bits (207), Expect = 2e-16
Identities = 55/161 (34%), Positives = 81/161 (50%), Gaps = 3/161 (1%)
Frame = +2
Query: 26 PIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGV-AVGRILPRKIA 202
P+IA++ G A GC+L CDI G ++F+ P IL G G + R + + +A
Sbjct: 129 PVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLA 188
Query: 203 LEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLGKK--NFID 376
+EM+ TGD ISA+ A + GL + + A KIAS S V+ + K+ N
Sbjct: 189 MEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAF 248
Query: 377 QMTLQKEKAYEHTSNVMVENLQLSDCKEGIKAFLEKRKPNW 499
+MTL + E + D KEG+ AF+EKRK N+
Sbjct: 249 EMTLTEGSKLE--KKLFYSTFATDDRKEGMTAFVEKRKANF 287
>sp|Q8BH95|ECHM_MOUSE Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 83.2 bits (204), Expect = 4e-16
Identities = 54/161 (33%), Positives = 80/161 (49%), Gaps = 3/161 (1%)
Frame = +2
Query: 26 PIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGV-AVGRILPRKIA 202
P+IA++ G A GC+L CDI G ++F P IL G G + R + + +A
Sbjct: 129 PVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLA 188
Query: 203 LEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLGKK--NFID 376
+EM+ TGD ISA+ A + GL + + A KIAS S V+ + K+ N
Sbjct: 189 MEMVLTGDRISAQDAKQAGLVSKIFPVEKLVEEAIQCAEKIASNSKIVVAMAKESVNAAF 248
Query: 377 QMTLQKEKAYEHTSNVMVENLQLSDCKEGIKAFLEKRKPNW 499
+MTL + E + D +EG+ AF+EKRK N+
Sbjct: 249 EMTLTEGNKLE--KRLFYSTFATDDRREGMTAFVEKRKANF 287
>sp|Q5R646|ECHM_PONPY Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 83.2 bits (204), Expect = 4e-16
Identities = 57/169 (33%), Positives = 85/169 (50%), Gaps = 3/169 (1%)
Frame = +2
Query: 2 TSIEKYFIPIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGV-AVG 178
T I+K P+IA++ G A GC+L CDI G ++F+ P IL G G +
Sbjct: 124 TQIKK---PVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLT 180
Query: 179 RILPRKIALEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLG 358
R + + +A+EM+ TGD ISA+ A + GL + + A KIAS S + +
Sbjct: 181 RTVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIIAAMA 240
Query: 359 KK--NFIDQMTLQKEKAYEHTSNVMVENLQLSDCKEGIKAFLEKRKPNW 499
K+ N +MTL + E + +D KEG+ AF+EKRK N+
Sbjct: 241 KESVNAAFEMTLAEGSKLE--KKLFYSTFATNDRKEGMTAFVEKRKANF 287
>sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 82.0 bits (201), Expect = 8e-16
Identities = 52/161 (32%), Positives = 80/161 (49%), Gaps = 3/161 (1%)
Frame = +2
Query: 26 PIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGV-AVGRILPRKIA 202
P+IA++ G A GC+L CDI G ++F P IL G G + R + + +A
Sbjct: 129 PVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLA 188
Query: 203 LEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLGKK--NFID 376
+EM+ TGD ISA+ A + GL + + A KIA+ S ++ + K+ N
Sbjct: 189 MEMVLTGDRISAQDAKQAGLVSKIFPVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAF 248
Query: 377 QMTLQKEKAYEHTSNVMVENLQLSDCKEGIKAFLEKRKPNW 499
+MTL + E + D +EG+ AF+EKRK N+
Sbjct: 249 EMTLTEGNKLE--KKLFYSTFATDDRREGMSAFVEKRKANF 287
>sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 82.0 bits (201), Expect = 8e-16
Identities = 55/161 (34%), Positives = 79/161 (49%), Gaps = 3/161 (1%)
Frame = +2
Query: 26 PIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGV-AVGRILPRKIA 202
P+IA++ G A GC+L CDI G ++F P IL G G + R + + +A
Sbjct: 129 PVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGKSLA 188
Query: 203 LEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLGKK--NFID 376
+EM+ TGD ISA+ A + GL + + A KIAS S V + K+ N
Sbjct: 189 MEMVLTGDRISAQDAKQAGLVSKIFPVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAF 248
Query: 377 QMTLQKEKAYEHTSNVMVENLQLSDCKEGIKAFLEKRKPNW 499
+MTL + E + D KEG+ AF+EKRK N+
Sbjct: 249 EMTLAEGVKLE--KKLFYSTFATEDRKEGMAAFVEKRKANF 287
>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
Length = 261
Score = 78.6 bits (192), Expect = 9e-15
Identities = 48/159 (30%), Positives = 78/159 (49%), Gaps = 4/159 (2%)
Frame = +2
Query: 26 PIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGVA----VGRILPR 193
P+IA++ G A GC++ SCDI I +++F P + G+ TPG + R++
Sbjct: 99 PVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQPEVGLGI---TPGFGGTQRLSRLVGM 155
Query: 194 KIALEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLGKKNFI 373
+A ++++T I A+ AL+ GL +++IANKI S + +KL K+
Sbjct: 156 GMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTAKEIANKIVSNAPVAVKLSKQAIN 215
Query: 374 DQMTLQKEKAYEHTSNVMVENLQLSDCKEGIKAFLEKRK 490
M + A S E D K+ + AF+EKRK
Sbjct: 216 RGMQCDIDTALAFESEAFGECFSTEDQKDAMTAFIEKRK 254
>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
Length = 288
Score = 74.3 bits (181), Expect = 2e-13
Identities = 48/169 (28%), Positives = 79/169 (46%), Gaps = 3/169 (1%)
Frame = +2
Query: 2 TSIEKYFIPIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGVAV-G 178
T++ P+IA++ G A G +L CDI G ++F P I G G
Sbjct: 119 TAVSDVKKPVIAAVNGFALGGGNELAMMCDIIYAGEKARFGQPEINIGTIPGAGGTQRWA 178
Query: 179 RILPRKIALEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLG 358
R + A+E+ TG+ ++A+ A ++G+ + K+ KIA S ++++
Sbjct: 179 RAAGKSFAMEVCLTGNHVTAQEAKEHGIVSKIFPADQVVGEAVKLGEKIADQSPLIVQMA 238
Query: 359 KK--NFIDQMTLQKEKAYEHTSNVMVENLQLSDCKEGIKAFLEKRKPNW 499
K+ N ++TLQ+ +E + D KEG+ AF EKRKP W
Sbjct: 239 KEAVNKAYELTLQEGLHFER--RLFHATFATKDRKEGMTAFAEKRKPQW 285
>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
Length = 255
Score = 69.7 bits (169), Expect = 4e-12
Identities = 41/159 (25%), Positives = 76/159 (47%), Gaps = 1/159 (0%)
Frame = +2
Query: 26 PIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGV-AVGRILPRKIA 202
P+IA++ G A AGC+L CD+ + G +++F P I G+ G + R + + +A
Sbjct: 94 PLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLA 153
Query: 203 LEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLGKKNFIDQM 382
+M+ +G+ I+A+ A + GL + ++A+K+A S ++ K+
Sbjct: 154 SKMVLSGESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQ 213
Query: 383 TLQKEKAYEHTSNVMVENLQLSDCKEGIKAFLEKRKPNW 499
+ + + D EGI AFL+KR P++
Sbjct: 214 EVALQAGLAQERQLFTLLAATEDRHEGISAFLQKRTPDF 252
>sp|Q8Z9L5|CAID_SALTI Carnitinyl-CoA dehydratase (Crotonobetainyl-CoA hydratase)
Length = 261
Score = 69.7 bits (169), Expect = 4e-12
Identities = 43/163 (26%), Positives = 80/163 (49%), Gaps = 5/163 (3%)
Frame = +2
Query: 26 PIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGV-AVGRILPRKIA 202
P+IA++ G A G +L + D +C ++ F+ P G+ + GV + ++LP I
Sbjct: 96 PVIAAVNGYAFGGGFELALAADFIVCAENASFALPEAKLGIVPDSGGVLRLPKLLPPAIV 155
Query: 203 LEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLGKKNFIDQM 382
EM+ TG +SAE AL++G+ + ++A ++ + + I K+ +
Sbjct: 156 NEMVMTGRRMSAEEALRWGIVNRVVSQSELMESARELAQQLVNSAPLAIAALKEIYRATS 215
Query: 383 TLQKEKAYEHTSNVMVEN----LQLSDCKEGIKAFLEKRKPNW 499
+ E+ Y + + ++++ L D EG +AF EKR P W
Sbjct: 216 EMPVEEGYRYIRSGVLKHYPSVLHSEDALEGPQAFAEKRAPVW 258
>sp|Q8ZRX5|CAID_SALTY Carnitinyl-CoA dehydratase (Crotonobetainyl-CoA hydratase)
Length = 261
Score = 69.3 bits (168), Expect = 6e-12
Identities = 43/163 (26%), Positives = 80/163 (49%), Gaps = 5/163 (3%)
Frame = +2
Query: 26 PIIASIQGPAAAAGCQLVASCDIAICGRSSKFSTPGILAGLFCSTPGV-AVGRILPRKIA 202
P+IA++ G A G +L + D +C ++ F+ P G+ + GV + ++LP I
Sbjct: 96 PVIAAVNGYAFGGGFELALAADFIVCAENASFALPEAKLGIVPDSGGVLRLPKLLPPAIV 155
Query: 203 LEMMYTGDPISAETALKYGLXXXXXXXXXXXXMSEKIANKIASLSSTVIKLGKKNFIDQM 382
EM+ TG +SAE AL++G+ + ++A ++ + + I K+ +
Sbjct: 156 NEMVMTGRRMSAEEALRWGVVNRVVSQSELMESARELAQQLVNSAPLAIAALKEIYRATS 215
Query: 383 TLQKEKAYEHTSNVMVEN----LQLSDCKEGIKAFLEKRKPNW 499
+ E+ Y + + ++++ L D EG +AF EKR P W
Sbjct: 216 EMPVEEGYRYIRSGVLKHYPSVLHSEDALEGPQAFAEKRDPVW 258
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,982,035
Number of Sequences: 369166
Number of extensions: 1161607
Number of successful extensions: 3200
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3098
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3170
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3783975580
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)