Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_F03
(880 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UGP8|SEC63_HUMAN Translocation protein SEC63 homolog 163 5e-40
sp|Q8VHE0|SEC63_MOUSE Translocation protein SEC63 homolog 163 5e-40
sp|P14906|SEC63_YEAST Translocation protein (NPL1 protein) 87 6e-17
sp|Q04960|DNJH_CUCSA DnaJ protein homolog (DNAJ-1) 59 2e-08
sp|P42824|DNJH2_ALLPO DnaJ protein homolog 2 58 4e-08
sp|P43644|DNJH_ATRNU DnaJ protein homolog ANJ1 56 1e-07
sp|Q94AW8|DNAJ3_ARATH Chaperone protein dnaJ 3 (AtJ3) (AtDjA3) 56 1e-07
sp|P42825|DNAJ2_ARATH Chaperone protein dnaJ 2 (AtDjA2) 55 3e-07
sp|P25294|SIS1_YEAST SIS1 protein 54 6e-07
sp|Q823T2|DNAJ_CHLCV Chaperone protein dnaJ 53 1e-06
>sp|Q9UGP8|SEC63_HUMAN Translocation protein SEC63 homolog
Length = 760
Score = 163 bits (413), Expect = 5e-40
Identities = 81/207 (39%), Positives = 122/207 (58%), Gaps = 23/207 (11%)
Frame = +1
Query: 10 MQGNQFEFDETGESFYYFLITLYLFALIPITYFYWPKIDNDV------------------ 135
M G QF++D++G +F+YFL + +IP TY+ WP+ N
Sbjct: 1 MAGQQFQYDDSGNTFFYFLTSFVGLIVIPATYYLWPRDQNAEQIRLKNIRKVYGRCMWYR 60
Query: 136 --LLESNQNFMNRKKKFIRANFIIAWIVFFWLVYKISQIETEWKEFNPFTELGVSKEANM 309
LL+ N + KK + + W +F +L YK+S+ + E++E+NP+ L + A +
Sbjct: 61 LRLLKPQPNIIPTVKKIV---LLAGWALFLFLAYKVSKTDREYQEYNPYEVLNLDPGATV 117
Query: 310 SEIKNAYEQLSLKFHPEKGGDPKRFIRLTKSFMILNNEKYRNNWEKYGNPNGPRIIRIGF 489
+EIK Y LSLK+HP+KGGD F+R+ K++ L +E+ R NWE++GNP+GP+ G
Sbjct: 118 AEIKKQYRLLSLKYHPDKGGDEVMFMRIAKAYAALTDEESRKNWEEFGNPDGPQATSFGI 177
Query: 490 ALPKWFVDKKNSVLVLGVY---FVVIL 561
ALP W VD+KNS+LVL VY F+VIL
Sbjct: 178 ALPAWIVDQKNSILVLLVYGLAFMVIL 204
Score = 35.8 bits (81), Expect = 0.16
Identities = 17/63 (26%), Positives = 35/63 (55%), Gaps = 2/63 (3%)
Frame = +1
Query: 697 DKNVMVKKYSFHINDMISIVAQFVTILHHSTTIYF--PQWEIIEYCMKLNEIFVRGISGI 870
D+ M+KK + +M++++ Q + + + F P +E CMKL+++ V+G+
Sbjct: 321 DQQFMLKKCPALLQEMVNVICQLIVMARNREEREFRAPTLASLENCMKLSQMAVQGLQQF 380
Query: 871 QSP 879
+SP
Sbjct: 381 KSP 383
>sp|Q8VHE0|SEC63_MOUSE Translocation protein SEC63 homolog
Length = 760
Score = 163 bits (413), Expect = 5e-40
Identities = 81/207 (39%), Positives = 122/207 (58%), Gaps = 23/207 (11%)
Frame = +1
Query: 10 MQGNQFEFDETGESFYYFLITLYLFALIPITYFYWPKIDNDV------------------ 135
M G QF++D++G +F+YFL + +IP TY+ WP+ N
Sbjct: 1 MAGQQFQYDDSGNTFFYFLTSFVGLIVIPATYYLWPRDQNAEQIRLKNIRKVYGRCMWYR 60
Query: 136 --LLESNQNFMNRKKKFIRANFIIAWIVFFWLVYKISQIETEWKEFNPFTELGVSKEANM 309
LL+ N + KK + + W +F +L YK+S+ + E++E+NP+ L + A +
Sbjct: 61 LRLLKPQPNIIPTVKKIV---LLAGWALFLFLAYKVSKTDREYQEYNPYEVLNLDPGATV 117
Query: 310 SEIKNAYEQLSLKFHPEKGGDPKRFIRLTKSFMILNNEKYRNNWEKYGNPNGPRIIRIGF 489
+EIK Y LSLK+HP+KGGD F+R+ K++ L +E+ R NWE++GNP+GP+ G
Sbjct: 118 AEIKKQYRLLSLKYHPDKGGDEVMFMRIAKAYAALTDEESRKNWEEFGNPDGPQATSFGI 177
Query: 490 ALPKWFVDKKNSVLVLGVY---FVVIL 561
ALP W VD+KNS+LVL VY F+VIL
Sbjct: 178 ALPAWIVDQKNSILVLLVYGLAFMVIL 204
Score = 34.7 bits (78), Expect = 0.36
Identities = 17/63 (26%), Positives = 34/63 (53%), Gaps = 2/63 (3%)
Frame = +1
Query: 697 DKNVMVKKYSFHINDMISIVAQFVTILHHSTTIYF--PQWEIIEYCMKLNEIFVRGISGI 870
D+ M+KK + +M++++ Q + + F P +E CMKL+++ V+G+
Sbjct: 321 DQQFMLKKCPALLQEMVNVICQLIIMARSREEREFRAPTLASLENCMKLSQMAVQGLQQF 380
Query: 871 QSP 879
+SP
Sbjct: 381 KSP 383
>sp|P14906|SEC63_YEAST Translocation protein (NPL1 protein)
Length = 663
Score = 87.0 bits (214), Expect = 6e-17
Identities = 65/230 (28%), Positives = 113/230 (49%), Gaps = 49/230 (21%)
Frame = +1
Query: 25 FEFDETGESFYYFLITLYLFALIPITYFYWPKI-----------------DNDVLLESNQ 153
+E+DE E++ F++T L + P+T +I + +V N+
Sbjct: 5 YEYDEASETWPSFILTGLLMVVGPMTLLQIYQIFFGANAEDGNSGKSKEFNEEVFKNLNE 64
Query: 154 NFMNRKKKFIRANF------------------IIAWIVFFWLVYKISQ----IETEWKEF 267
+ + + K R F I+ WI+ L+ +I+ + K F
Sbjct: 65 EYTSDEIKQFRRKFDKNSNKKSKIWSRRNIIIIVGWILVAILLQRINSNDAIKDAATKLF 124
Query: 268 NPFTELGVSKEANMSEIKNAYEQLSLKFHPE---KGGDP-------KRFIRLTKSFMILN 417
+P+ LG+S A+ +IK+AY +LS+KFHP+ KG P + ++++TK++ L
Sbjct: 125 DPYEILGISTSASDRDIKSAYRKLSVKFHPDKLAKGLTPDEKSVMEETYVQITKAYESLT 184
Query: 418 NEKYRNNWEKYGNPNGPRIIRIGFALPKWFVDKKNSVLVLGVYFVVILGL 567
+E R N+ KYG+P+GP+ G ALP++ VD S L++ V +V +LGL
Sbjct: 185 DELVRQNYLKYGHPDGPQSTSHGIALPRFLVDGSASPLLV-VCYVALLGL 233
>sp|Q04960|DNJH_CUCSA DnaJ protein homolog (DNAJ-1)
Length = 413
Score = 58.5 bits (140), Expect = 2e-08
Identities = 23/57 (40%), Positives = 42/57 (73%)
Frame = +1
Query: 283 LGVSKEANMSEIKNAYEQLSLKFHPEKGGDPKRFIRLTKSFMILNNEKYRNNWEKYG 453
LGVSK A+ ++K AY + ++K HP+KGGDP++F L +++ +L++ + R +++YG
Sbjct: 17 LGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYG 73
>sp|P42824|DNJH2_ALLPO DnaJ protein homolog 2
Length = 418
Score = 57.8 bits (138), Expect = 4e-08
Identities = 23/57 (40%), Positives = 41/57 (71%)
Frame = +1
Query: 283 LGVSKEANMSEIKNAYEQLSLKFHPEKGGDPKRFIRLTKSFMILNNEKYRNNWEKYG 453
LGVSK A ++K AY + ++K HP+KGGDP++F + +++ +LN+ + R +++YG
Sbjct: 18 LGVSKNATPEDLKKAYRKAAIKNHPDKGGDPEKFKEIGQAYEVLNDPEKREIYDQYG 74
>sp|P43644|DNJH_ATRNU DnaJ protein homolog ANJ1
Length = 417
Score = 56.2 bits (134), Expect = 1e-07
Identities = 22/57 (38%), Positives = 42/57 (73%)
Frame = +1
Query: 283 LGVSKEANMSEIKNAYEQLSLKFHPEKGGDPKRFIRLTKSFMILNNEKYRNNWEKYG 453
LGV K+A+ ++K AY++ ++K HP+KGGDP++F L ++ +L++ + R +++YG
Sbjct: 18 LGVPKDASPEDLKKAYKKAAIKNHPDKGGDPEKFKELAHAYEVLSDPEKREIYDQYG 74
>sp|Q94AW8|DNAJ3_ARATH Chaperone protein dnaJ 3 (AtJ3) (AtDjA3)
Length = 420
Score = 56.2 bits (134), Expect = 1e-07
Identities = 22/57 (38%), Positives = 42/57 (73%)
Frame = +1
Query: 283 LGVSKEANMSEIKNAYEQLSLKFHPEKGGDPKRFIRLTKSFMILNNEKYRNNWEKYG 453
LGV K A+ ++K AY++ ++K HP+KGGDP++F L +++ +L++ + R +++YG
Sbjct: 19 LGVPKSASPEDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYG 75
>sp|P42825|DNAJ2_ARATH Chaperone protein dnaJ 2 (AtDjA2)
Length = 419
Score = 54.7 bits (130), Expect = 3e-07
Identities = 22/57 (38%), Positives = 41/57 (71%)
Frame = +1
Query: 283 LGVSKEANMSEIKNAYEQLSLKFHPEKGGDPKRFIRLTKSFMILNNEKYRNNWEKYG 453
LGV K A ++K AY++ ++K HP+KGGDP++F L +++ +L++ + R +++YG
Sbjct: 19 LGVPKTAAPEDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYG 75
>sp|P25294|SIS1_YEAST SIS1 protein
Length = 352
Score = 53.9 bits (128), Expect = 6e-07
Identities = 26/66 (39%), Positives = 43/66 (65%), Gaps = 1/66 (1%)
Frame = +1
Query: 259 KEFNPFTELGVSKEANMSEIKNAYEQLSLKFHPEK-GGDPKRFIRLTKSFMILNNEKYRN 435
KE + LGVS AN E+K Y + +LK+HP+K GD ++F ++++F ILN+ + R
Sbjct: 3 KETKLYDLLGVSPSANEQELKKGYRKAALKYHPDKPTGDTEKFKEISEAFEILNDPQKRE 62
Query: 436 NWEKYG 453
+++YG
Sbjct: 63 IYDQYG 68
>sp|Q823T2|DNAJ_CHLCV Chaperone protein dnaJ
Length = 392
Score = 53.1 bits (126), Expect = 1e-06
Identities = 27/66 (40%), Positives = 47/66 (71%), Gaps = 4/66 (6%)
Frame = +1
Query: 283 LGVSKEANMSEIKNAYEQLSLKFHPEKG-GD---PKRFIRLTKSFMILNNEKYRNNWEKY 450
LGVSK A+ EIK +Y +L++K+HP+K GD KRF +++++ +L++ + R ++++Y
Sbjct: 7 LGVSKTASPEEIKKSYRKLAVKYHPDKNPGDAEAEKRFKEVSEAYEVLSDPQKRESYDRY 66
Query: 451 GNPNGP 468
G NGP
Sbjct: 67 GK-NGP 71
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,340,468
Number of Sequences: 369166
Number of extensions: 1883413
Number of successful extensions: 5330
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5296
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8742211660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)