Planarian EST Database


Dr_sW_019_F01

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_019_F01
         (494 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q94546|ATU_DROME  Another transcription unit protein            50   3e-06
sp|Q9QX47|SON_MOUSE  SON protein                                   33   0.28 
sp|P18583|SON_HUMAN  SON protein (SON3) (Negative regulatory...    33   0.37 
sp|Q5SXM2|SNPC4_HUMAN  snRNA-activating protein complex subu...    32   0.63 
sp|Q00839|HNRPU_HUMAN  Heterogenous nuclear ribonucleoprotei...    30   2.4  
sp|P08551|NFL_MOUSE  Neurofilament triplet L protein (68 kDa...    30   4.1  
sp|Q9UZ14|SYT_PYRAB  Threonyl-tRNA synthetase (Threonine--tR...    29   5.3  
sp|Q86NP2|NELFA_DROME  Negative elongation factor A homolog        29   5.3  
>sp|Q94546|ATU_DROME Another transcription unit protein
          Length = 725

 Score = 50.1 bits (118), Expect = 3e-06
 Identities = 29/105 (27%), Positives = 55/105 (52%), Gaps = 5/105 (4%)
 Frame = +3

Query: 3   DSFTHRKLTLSLADKTHKAAKVKLLPFAGENPDVRQQEMIKREEERMKASLXXXXXXXXX 182
           +SFTH+K+T+SLAD++ K + +K+L   G++P   +   ++ EE +++ ++         
Sbjct: 519 ESFTHKKMTMSLADRSSKTSGIKILTQVGKDPTTDRPTQLREEEAKLRQAMRNQHKSLPK 578

Query: 183 XXXXXXXEL-----TAAFLEQQKDDENNPHAISLSDIKKKARPSS 302
                  E      T+++   +  D+ N  AISLS IK + +  S
Sbjct: 579 KKKPGAGEPLIGGGTSSYQHDEGSDDEN--AISLSAIKNRYKKGS 621
>sp|Q9QX47|SON_MOUSE SON protein
          Length = 2404

 Score = 33.5 bits (75), Expect = 0.28
 Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 2/58 (3%)
 Frame = +1

Query: 265  RCRISRRRRVPAPQSKRKEPSICPRRMRRKRSP--*AISQIPLRKRKERKLELSQTTK 432
            R R   RRR      +R+  SI P R+RR R+P     S+ P+R+++ R  E  ++ K
Sbjct: 1969 RSRTPSRRRRSRSAVRRRSFSISPVRLRRSRTPLRRRFSRSPIRRKRSRSSERGRSPK 2026
>sp|P18583|SON_HUMAN SON protein (SON3) (Negative regulatory element-binding protein)
            (NRE-binding protein) (DBP-5) (Bax antagonist selected in
            saccharomyces 1) (BASS1)
          Length = 2426

 Score = 33.1 bits (74), Expect = 0.37
 Identities = 20/58 (34%), Positives = 31/58 (53%), Gaps = 2/58 (3%)
 Frame = +1

Query: 265  RCRISRRRRVPAPQSKRKEPSICPRRMRRKRSP--*AISQIPLRKRKERKLELSQTTK 432
            R R   RRR      +R+  SI P R+RR R+P     S+ P+R+++ R  E  ++ K
Sbjct: 1991 RSRTPSRRRRSRSVVRRRSFSISPVRLRRSRTPLRRRFSRSPIRRKRSRSSERGRSPK 2048
>sp|Q5SXM2|SNPC4_HUMAN snRNA-activating protein complex subunit 4 (SNAPc subunit 4)
           (snRNA-activating protein complex 190 kDa subunit)
           (SNAPc 190 kDa subunit) (Proximal sequence
           element-binding transcription factor alpha subunit)
           (PSE-binding factor alpha subunit) (PTF alpha subunit)
          Length = 1469

 Score = 32.3 bits (72), Expect = 0.63
 Identities = 19/68 (27%), Positives = 30/68 (44%), Gaps = 1/68 (1%)
 Frame = +2

Query: 104 PPAGDDQARGGANEGLLASRESAQTDAGAREPPRTHRSVPRAAEGR*EQSSRYFAVGYQE 283
           PP G   ++GG+ E    +    +  +  + P R H  VPR+A+      +R      Q 
Sbjct: 600 PPKGSSASQGGSKEASTTAAAPGEETSPVQVPARAHGPVPRSAQASHSADTRPAGAEKQA 659

Query: 284 -EGASQLL 304
            EG  +LL
Sbjct: 660 LEGGRRLL 667
>sp|Q00839|HNRPU_HUMAN Heterogenous nuclear ribonucleoprotein U (hnRNP U) (Scaffold
           attachment factor A) (SAF-A) (pp120)
          Length = 824

 Score = 30.4 bits (67), Expect = 2.4
 Identities = 29/126 (23%), Positives = 45/126 (35%), Gaps = 14/126 (11%)
 Frame = +2

Query: 113 GDDQARGGANEGLLASRESAQTDAGAREPPRTHRSVPRAAEGR*EQSSRYFAVGYQEEGA 292
           GD++   G   G     +  Q  A  ++ P+  R   + A G+    +  FAV     GA
Sbjct: 146 GDEEEGAGDENG--HGEQQPQPPATQQQQPQQQRGAAKEAAGKSSGPTSLFAVTVAPPGA 203

Query: 293 SQLLNQKEKNHRYVLDG*GGR--------------GVHERFLRSPSEKERKES*NYLRRR 430
            Q   Q     +    G GGR              G  +R ++ P E   +    Y+   
Sbjct: 204 RQGQQQAGGKKKAEGGGGGGRPGAPAGDGKTEQKGGDKKRGVKRpreDHGRGYFEYIEEN 263

Query: 431 KFQRIK 448
           K+ R K
Sbjct: 264 KYSRAK 269
>sp|P08551|NFL_MOUSE Neurofilament triplet L protein (68 kDa neurofilament protein)
           (Neurofilament light polypeptide) (NF-L)
          Length = 543

 Score = 29.6 bits (65), Expect = 4.1
 Identities = 21/82 (25%), Positives = 37/82 (45%)
 Frame = +2

Query: 164 ESAQTDAGAREPPRTHRSVPRAAEGR*EQSSRYFAVGYQEEGASQLLNQKEKNHRYVLDG 343
           E+ + +    EPP         +EG  E+  +    G +EEGA +    K+++     + 
Sbjct: 460 EATKAEEAKDEPP---------SEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEE 510

Query: 344 *GGRGVHERFLRSPSEKERKES 409
            GG G  E    S  E++++ES
Sbjct: 511 EGGEGEEEDTKESEEEEKKEES 532
>sp|Q9UZ14|SYT_PYRAB Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)
          Length = 625

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 12/36 (33%), Positives = 22/36 (61%)
 Frame = +3

Query: 204 ELTAAFLEQQKDDENNPHAISLSDIKKKARPSSSIK 311
           E+  AF+  +K DE NP  +SL  I++ ++ +  +K
Sbjct: 37  EVLVAFISVEKVDEKNPEEVSLKAIEEISKVAEQVK 72
>sp|Q86NP2|NELFA_DROME Negative elongation factor A homolog
          Length = 1248

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 12/25 (48%), Positives = 19/25 (76%)
 Frame = -1

Query: 107 ADIGVLpreGQQLNLGGLVSLVGER 33
           +D+G+LP E Q LN   L+S+VG++
Sbjct: 135 SDLGMLPLECQYLNKNALISVVGQQ 159
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,418,397
Number of Sequences: 369166
Number of extensions: 781540
Number of successful extensions: 2172
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2170
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3069744620
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)