Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_019_D19 (303 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P68586|MTBP_BPPHT Modification methylase Phi3TI (Cytosin... 33 0.21 sp||P09389_1 [Segment 1 of 2] Modification methylase SPBeta... 33 0.21 sp|Q57532|GYRA_MYCLE DNA gyrase subunit A [Contains: Mle gy... 29 3.0 sp|P17632|MBHL_RHOGE Uptake hydrogenase large subunit (Hydr... 29 3.9 sp|Q97AR2|TRM1_THEVO N(2),N(2)-dimethylguanosine tRNA methy... 29 3.9 sp|P31891|MBHL_RALEU Uptake hydrogenase large subunit (Hydr... 28 5.1 sp|P12636|MBHL_BRAJA Uptake hydrogenase large subunit (Hydr... 28 6.6 sp|P18636|MBHL_RHILV Uptake hydrogenase large subunit (Hydr... 28 6.6 sp|P75318|Y465_MYCPN Hypothetical protein MPN465 (P01_orf199) 28 6.6 sp|Q83B24|SYM_COXBU Methionyl-tRNA synthetase (Methionine--... 28 6.6
>sp|P68586|MTBP_BPPHT Modification methylase Phi3TI (Cytosine-specific methyltransferase Phi3TI) (M.Phi3TI) sp|P68585|MTBP_BACSU Phi-3T prophage-derived modification methylase Phi3TI (Cytosine-specific methyltransferase Phi3TI) (M.Phi3TI) Length = 443 Score = 33.1 bits (74), Expect = 0.21 Identities = 14/30 (46%), Positives = 20/30 (66%), Gaps = 2/30 (6%) Frame = -3 Query: 121 CSSCSF--IIRWESYRRFVKCPMCNLLPKA 38 CS+CS +I +E Y++ VKCP C + KA Sbjct: 94 CSNCSHEHLITYEDYKKGVKCPKCEAVSKA 123
>sp||P09389_1 [Segment 1 of 2] Modification methylase SPBetaI (Cytosine-specific methyltransferase SPBetaI) (M.SPBetaI) Length = 143 Score = 33.1 bits (74), Expect = 0.21 Identities = 14/30 (46%), Positives = 20/30 (66%), Gaps = 2/30 (6%) Frame = -3 Query: 121 CSSCSF--IIRWESYRRFVKCPMCNLLPKA 38 CS+CS +I +E Y++ VKCP C + KA Sbjct: 94 CSNCSHEHLITYEDYKKGVKCPKCEAVSKA 123
>sp|Q57532|GYRA_MYCLE DNA gyrase subunit A [Contains: Mle gyrA intein] Length = 1273 Score = 29.3 bits (64), Expect = 3.0 Identities = 18/44 (40%), Positives = 26/44 (59%), Gaps = 1/44 (2%) Frame = +3 Query: 21 NSLVRVAFGKRLHIGHF-TKRRYDSQRIIKEQLEQINDHGKPSV 149 NSLVR+ FGK + IG T +++S I L+ ++ HG P V Sbjct: 135 NSLVRLLFGKSIRIGDIVTGAQFNSDNPI--DLKVLDRHGNPVV 176
>sp|P17632|MBHL_RHOGE Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound hydrogenase large subunit) Length = 618 Score = 28.9 bits (63), Expect = 3.9 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +3 Query: 120 QINDHGKPSVHLHALFWVASISGL 191 Q++DH HLHAL WV +S L Sbjct: 114 QVHDHAVHFYHLHALDWVDVVSAL 137
>sp|Q97AR2|TRM1_THEVO N(2),N(2)-dimethylguanosine tRNA methyltransferase (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA 2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2,2)G26)dimethyltransferase) Length = 342 Score = 28.9 bits (63), Expect = 3.9 Identities = 15/44 (34%), Positives = 22/44 (50%) Frame = +3 Query: 3 YKSYMMNSLVRVAFGKRLHIGHFTKRRYDSQRIIKEQLEQINDH 134 Y +Y++N + R G R IG+ +R R I+ QL N H Sbjct: 162 YGAYIVNDIYRHEMGIRTLIGYIARRAAAFDRYIEPQLSIWNHH 205
>sp|P31891|MBHL_RALEU Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound hydrogenase large subunit) Length = 618 Score = 28.5 bits (62), Expect = 5.1 Identities = 14/32 (43%), Positives = 18/32 (56%) Frame = +3 Query: 96 RIIKEQLEQINDHGKPSVHLHALFWVASISGL 191 R I + Q++DH HLHAL WV +S L Sbjct: 106 REIMAKTLQVHDHAVHFYHLHALDWVDVMSAL 137
>sp|P12636|MBHL_BRAJA Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound hydrogenase large subunit) Length = 596 Score = 28.1 bits (61), Expect = 6.6 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = +3 Query: 87 DSQRIIKEQLEQINDHGKPSVHLHALFWVASISGL 191 +S R + + Q++DH HLHAL WV +S L Sbjct: 103 NSIRNLMQLALQVHDHVVHFYHLHALDWVDVVSAL 137
>sp|P18636|MBHL_RHILV Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound hydrogenase large subunit) Length = 596 Score = 28.1 bits (61), Expect = 6.6 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = +3 Query: 87 DSQRIIKEQLEQINDHGKPSVHLHALFWVASISGL 191 +S R + + Q++DH HLHAL WV +S L Sbjct: 103 NSIRNLMQLALQVHDHVVHFYHLHALDWVDVVSAL 137
>sp|P75318|Y465_MYCPN Hypothetical protein MPN465 (P01_orf199) Length = 199 Score = 28.1 bits (61), Expect = 6.6 Identities = 12/27 (44%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Frame = -3 Query: 163 SACKCTLGL-PWSLICSSCSFIIRWES 86 SAC+ L + PW+ ICS +F W S Sbjct: 46 SACRIDLSVFPWAFICSPWNFCSTWSS 72
>sp|Q83B24|SYM_COXBU Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS) Length = 546 Score = 28.1 bits (61), Expect = 6.6 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = +3 Query: 111 QLEQINDHGKPSVHLHALFWVASISG 188 Q E + GK V+ HALFW A +SG Sbjct: 286 QTELYHFVGKDIVYFHALFWPAMLSG 311
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 35,705,715 Number of Sequences: 369166 Number of extensions: 667210 Number of successful extensions: 1621 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 1601 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1621 length of database: 68,354,980 effective HSP length: 69 effective length of database: 55,608,265 effective search space used: 1723856215 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)