Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_D05-2
(255 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9X7L2|LEU1_RHIME 2-isopropylmalate synthase (Alpha-isop... 35 0.041
sp|Q8UD63|LEU1_AGRT5 2-isopropylmalate synthase (Alpha-isop... 33 0.20
sp|Q89AX5|6PGD_BUCBP 6-phosphogluconate dehydrogenase, deca... 30 2.2
sp|Q82BV3|LEU1_STRAW 2-isopropylmalate synthase (Alpha-isop... 28 5.0
sp|Q9PP78|SYE2_CAMJE Glutamyl-tRNA synthetase 2 (Glutamate-... 28 6.5
sp|Q9W4C5|Y252_DROME Hypothetical sodium-dependent transpor... 28 6.5
sp|O31046|LEU1_STRCO 2-isopropylmalate synthase (Alpha-isop... 28 8.5
sp|Q97I63|RRF_CLOAB Ribosome recycling factor (Ribosome rel... 28 8.5
sp|Q8EVM0|Y544_MYCPE Hypothetical UPF0144 protein MYPE5440 28 8.5
>sp|Q9X7L2|LEU1_RHIME 2-isopropylmalate synthase (Alpha-isopropylmalate synthase)
(Alpha-IPM synthetase)
Length = 558
Score = 35.4 bits (80), Expect = 0.041
Identities = 18/58 (31%), Positives = 28/58 (48%)
Frame = +1
Query: 46 TCQLKALKEVISCQLDASNVERISTDYRSSNHLLSDLADPYFRELLSMTFEDMHKSAL 219
T L + + +LD S++ERI Y SN ++ PY EL+ F H+ A+
Sbjct: 283 TLALNMFTQGVDPKLDCSDIERIKEVYEYSNQMVIPERHPYVGELVYTAFSGSHQDAI 340
>sp|Q8UD63|LEU1_AGRT5 2-isopropylmalate synthase (Alpha-isopropylmalate synthase)
(Alpha-IPM synthetase)
Length = 558
Score = 33.1 bits (74), Expect = 0.20
Identities = 17/58 (29%), Positives = 26/58 (44%)
Frame = +1
Query: 46 TCQLKALKEVISCQLDASNVERISTDYRSSNHLLSDLADPYFRELLSMTFEDMHKSAL 219
T L + + +LD ++ERI Y SN + PY EL+ F H+ A+
Sbjct: 283 TLALNMYTQGVDPELDCRDIERIKAVYEYSNEMTIPERHPYVGELVYTAFSGSHQDAI 340
>sp|Q89AX5|6PGD_BUCBP 6-phosphogluconate dehydrogenase, decarboxylating
Length = 468
Score = 29.6 bits (65), Expect = 2.2
Identities = 12/33 (36%), Positives = 22/33 (66%)
Frame = +1
Query: 82 CQLDASNVERISTDYRSSNHLLSDLADPYFREL 180
C + A ++ I+ +Y S+N+ ++ L PYFRE+
Sbjct: 364 CIIRAKLLKNITQEYSSNNNFVNLLLTPYFREI 396
>sp|Q82BV3|LEU1_STRAW 2-isopropylmalate synthase (Alpha-isopropylmalate synthase)
(Alpha-IPM synthetase)
Length = 573
Score = 28.5 bits (62), Expect = 5.0
Identities = 13/59 (22%), Positives = 26/59 (44%)
Frame = +1
Query: 46 TCQLKALKEVISCQLDASNVERISTDYRSSNHLLSDLADPYFRELLSMTFEDMHKSALK 222
T + + + Q+D SN++ + N + PY +L+ +F H+ A+K
Sbjct: 294 TLGMNLFSQGVDPQIDFSNIDEVRRTAEYCNQMEVHARHPYVGDLVYTSFSGSHQDAIK 352
>sp|Q9PP78|SYE2_CAMJE Glutamyl-tRNA synthetase 2 (Glutamate--tRNA ligase 2) (GluRS 2)
Length = 431
Score = 28.1 bits (61), Expect = 6.5
Identities = 19/62 (30%), Positives = 28/62 (45%)
Frame = +1
Query: 37 TQYTCQLKALKEVISCQLDASNVERISTDYRSSNHLLSDLADPYFRELLSMTFEDMHKSA 216
TQ T +K LKE + +A + T+Y+ LL D+ F E ++KS
Sbjct: 334 TQETSTIKELKEKMQAIFNAKDFGEFETEYKILKELLKDI--ELFENYEDFKNELLNKSN 391
Query: 217 LK 222
LK
Sbjct: 392 LK 393
>sp|Q9W4C5|Y252_DROME Hypothetical sodium-dependent transporter CG3252
Length = 641
Score = 28.1 bits (61), Expect = 6.5
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -1
Query: 123 ICTYSFYITRIKLTRYYFF*SFQ 55
IC S+Y + + LT YY F SFQ
Sbjct: 128 ICIISYYSSLLALTLYYLFVSFQ 150
>sp|O31046|LEU1_STRCO 2-isopropylmalate synthase (Alpha-isopropylmalate synthase)
(Alpha-IPM synthetase)
Length = 573
Score = 27.7 bits (60), Expect = 8.5
Identities = 13/59 (22%), Positives = 27/59 (45%)
Frame = +1
Query: 46 TCQLKALKEVISCQLDASNVERISTDYRSSNHLLSDLADPYFRELLSMTFEDMHKSALK 222
T + + + Q+D S+++ I + N + PY +L+ +F H+ A+K
Sbjct: 294 TLGMNLFSQGVDPQIDFSDIDEIRRTWEYCNQMEVHPRHPYVGDLVYTSFSGSHQDAIK 352
>sp|Q97I63|RRF_CLOAB Ribosome recycling factor (Ribosome releasing factor) (RRF)
Length = 185
Score = 27.7 bits (60), Expect = 8.5
Identities = 23/75 (30%), Positives = 37/75 (49%), Gaps = 4/75 (5%)
Frame = +1
Query: 13 SDYFKIKETQYTCQLKALK-EVISCQLDASN---VERISTDYRSSNHLLSDLADPYFREL 180
SD K E + T + ALK E+ S + +N ++RI DY + L+ LA+ E
Sbjct: 3 SDIIKELEEKMTKSISALKKELTSMKAGRANPAMLDRIEVDYYGTMTPLNQLANISVPES 62
Query: 181 LSMTFEDMHKSALKN 225
+ + KSA+K+
Sbjct: 63 RVLMIQPWDKSAMKS 77
>sp|Q8EVM0|Y544_MYCPE Hypothetical UPF0144 protein MYPE5440
Length = 469
Score = 27.7 bits (60), Expect = 8.5
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = +1
Query: 13 SDYFKIKETQYTCQLKALKEVISCQLDASNVERI 114
+DY KIKETQ + Q L+EVIS N+E+I
Sbjct: 80 ADYLKIKETQESYQ--QLQEVISEYQKKLNLEKI 111
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,936,678
Number of Sequences: 369166
Number of extensions: 366532
Number of successful extensions: 1007
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1001
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1007
length of database: 68,354,980
effective HSP length: 55
effective length of database: 58,194,555
effective search space used: 1687642095
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)