Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_C10
(547 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99KI0|ACON_MOUSE Aconitate hydratase, mitochondrial pre... 217 1e-56
sp|Q9ER34|ACON_RAT Aconitate hydratase, mitochondrial precu... 216 2e-56
sp|P20004|ACON_BOVIN Aconitate hydratase, mitochondrial pre... 216 4e-56
sp|Q99798|ACON_HUMAN Aconitate hydratase, mitochondrial pre... 215 6e-56
sp|P16276|ACON_PIG Aconitate hydratase, mitochondrial precu... 213 2e-55
sp|P34455|ACON_CAEEL Probable aconitate hydratase, mitochon... 207 1e-53
sp|P49609|ACON_GRAVE Aconitate hydratase, mitochondrial pre... 193 3e-49
sp|P19414|ACON_YEAST Aconitate hydratase, mitochondrial pre... 184 2e-46
sp|O13966|ACON_SCHPO Aconitate hydratase, mitochondrial pre... 171 1e-42
sp|P39533|ACON2_YEAST Putative aconitase in PRP21-UBP12 int... 142 4e-34
>sp|Q99KI0|ACON_MOUSE Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 217 bits (553), Expect = 1e-56
Identities = 101/139 (72%), Positives = 117/139 (84%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP TAR YKK GI WVVIGDENYGEGSSREHAALEPRHLGGR II KSFARIHETN
Sbjct: 639 EFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETN 698
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKEVIILNHTF 360
LKKQG+LPLTF+ P DYNKI P DK+++ L FAP KP++C IKH +G +E I+LNHTF
Sbjct: 699 LKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLKCVIKHPNGTQETILLNHTF 758
Query: 361 NKNQIDWFKAGSALNRMRE 417
N+ QI+WF+AGSALNRM+E
Sbjct: 759 NETQIEWFRAGSALNRMKE 777
>sp|Q9ER34|ACON_RAT Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 216 bits (551), Expect = 2e-56
Identities = 101/139 (72%), Positives = 116/139 (83%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP TAR YKK GI WVVIGDENYGEGSSREHAALEPRHLGGR II KSFARIHETN
Sbjct: 639 EFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETN 698
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKEVIILNHTF 360
LKKQG+LPLTF+ P DYNKI P DK+++ L FAP KP+ C IKH +G +E I+LNHTF
Sbjct: 699 LKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLNCIIKHPNGTQETILLNHTF 758
Query: 361 NKNQIDWFKAGSALNRMRE 417
N+ QI+WF+AGSALNRM+E
Sbjct: 759 NETQIEWFRAGSALNRMKE 777
>sp|P20004|ACON_BOVIN Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 216 bits (549), Expect = 4e-56
Identities = 101/139 (72%), Positives = 116/139 (83%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP TAR YKK GI WVVIGDENYGEGSSREHAALEPRHLGGR II KSFARIHETN
Sbjct: 639 EFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETN 698
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKEVIILNHTF 360
LKKQG+LPLTF+ P DYNKI P DK+++ L FAP KP+ C IKH +G +E I+LNHTF
Sbjct: 699 LKKQGLLPLTFADPADYNKIHPVDKLTIKGLKDFAPGKPLTCIIKHPNGTQETILLNHTF 758
Query: 361 NKNQIDWFKAGSALNRMRE 417
N+ QI+WF+AGSALNRM+E
Sbjct: 759 NETQIEWFRAGSALNRMKE 777
>sp|Q99798|ACON_HUMAN Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 780
Score = 215 bits (547), Expect = 6e-56
Identities = 100/139 (71%), Positives = 116/139 (83%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP TAR YKK GI WVVIGDENYGEGSSREHAALEPRHLGGR II KSFARIHETN
Sbjct: 639 EFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETN 698
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKEVIILNHTF 360
LKKQG+LPLTF+ P DYNKI P DK+++ L F P KP++C IKH +G +E I+LNHTF
Sbjct: 699 LKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFTPGKPLKCIIKHPNGTQETILLNHTF 758
Query: 361 NKNQIDWFKAGSALNRMRE 417
N+ QI+WF+AGSALNRM+E
Sbjct: 759 NETQIEWFRAGSALNRMKE 777
>sp|P16276|ACON_PIG Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 781
Score = 213 bits (542), Expect = 2e-55
Identities = 99/139 (71%), Positives = 116/139 (83%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP TAR YK+ GI WVVIGDENYGEGSSREH ALEPRHLGGR II KSFARIHETN
Sbjct: 639 EFGPVPDTARYYKQHGIRWVVIGDENYGEGSSREHRALEPRHLGGRAIITKSFARIHETN 698
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKEVIILNHTF 360
LKKQG+LPLTF+ P DYNKI P DK+++ L FAP KP++C IKH +G +E I+LNHTF
Sbjct: 699 LKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFAPGKPLKCIIKHPNGTQETILLNHTF 758
Query: 361 NKNQIDWFKAGSALNRMRE 417
N+ QI+WF+AGSALNRM+E
Sbjct: 759 NETQIEWFRAGSALNRMKE 777
>sp|P34455|ACON_CAEEL Probable aconitate hydratase, mitochondrial precursor (Citrate
hydro-lyase) (Aconitase)
Length = 777
Score = 207 bits (528), Expect = 1e-53
Identities = 97/139 (69%), Positives = 114/139 (82%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP+TAR YK G+ WV IGDENYGEGSSREHAALEPRHLGGR IIVKSFARIHETN
Sbjct: 633 EYGAVPATARKYKADGVRWVAIGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETN 692
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKEVIILNHTF 360
LKKQGMLPLTF+ P DY+KI PSD +S++ L+ FAP KP+ K +G+K + LNHTF
Sbjct: 693 LKKQGMLPLTFANPADYDKIDPSDNVSIVGLSSFAPGKPLTAIFKKTNGSKVEVTLNHTF 752
Query: 361 NKNQIDWFKAGSALNRMRE 417
N+ QI+WFKAGSALNRM+E
Sbjct: 753 NEQQIEWFKAGSALNRMKE 771
>sp|P49609|ACON_GRAVE Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 779
Score = 193 bits (490), Expect = 3e-49
Identities = 90/138 (65%), Positives = 107/138 (77%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP TAR YK G+ WVVIGDENYGEGSSREHAALEPRHLGG +IVKSFARIHETN
Sbjct: 637 EYGTVPDTARAYKAEGVKWVVIGDENYGEGSSREHAALEPRHLGGVAVIVKSFARIHETN 696
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKEVIILNHTF 360
LKKQGMLPLTF+ P DY+KI SDK+SL+ L P +PV + DG I+LNHTF
Sbjct: 697 LKKQGMLPLTFNNPADYDKIDSSDKVSLVGLKNLTPGEPVTMTVTKADGTSMDILLNHTF 756
Query: 361 NKNQIDWFKAGSALNRMR 414
N Q++WF+AGSALN+++
Sbjct: 757 NDEQLEWFRAGSALNKIK 774
>sp|P19414|ACON_YEAST Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 778
Score = 184 bits (466), Expect = 2e-46
Identities = 87/138 (63%), Positives = 104/138 (75%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP TAR+Y+ GI WVVIGDEN+GEGSSREHAALEPR LGG II KSFARIHETN
Sbjct: 636 EYKGVPDTARDYRDQGIKWVVIGDENFGEGSSREHAALEPRFLGGFAIITKSFARIHETN 695
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKEVIILNHTF 360
LKKQG+LPL F P DY+KI P D+I +L LA+ AP KPV + K+G +L HTF
Sbjct: 696 LKKQGLLPLNFKNPADYDKINPDDRIDILGLAELAPGKPVTMRVHPKNGKPWDAVLTHTF 755
Query: 361 NKNQIDWFKAGSALNRMR 414
N QI+WFK GSALN+++
Sbjct: 756 NDEQIEWFKYGSALNKIK 773
>sp|O13966|ACON_SCHPO Aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase)
(Aconitase)
Length = 778
Score = 171 bits (432), Expect = 1e-42
Identities = 82/138 (59%), Positives = 103/138 (74%), Gaps = 1/138 (0%)
Frame = +1
Query: 1 DWSDVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETN 180
++ VP+ A +Y+ GI WV +G++N+GEGSSREHAALEPR+LGG +I KSFARIHETN
Sbjct: 634 EYKTVPNVAIDYRDHGIRWVTLGEQNFGEGSSREHAALEPRYLGGAAVITKSFARIHETN 693
Query: 181 LKKQGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEKPVECEIKHKDGAKE-VIILNHT 357
LKKQG+LPLTF+ P Y+KI P D + + L FAP KP+ + DG+ E LNHT
Sbjct: 694 LKKQGLLPLTFADPAAYDKISPFDTVDIDGLTTFAPGKPLTLVVHPADGSAEWSTKLNHT 753
Query: 358 FNKNQIDWFKAGSALNRM 411
FNK+QI+WFKAGSALN M
Sbjct: 754 FNKDQIEWFKAGSALNHM 771
>sp|P39533|ACON2_YEAST Putative aconitase in PRP21-UBP12 intergenic region
Length = 789
Score = 142 bits (359), Expect = 4e-34
Identities = 70/137 (51%), Positives = 90/137 (65%), Gaps = 5/137 (3%)
Frame = +1
Query: 10 DVPSTARNYKKAGIPWVVIGDENYGEGSSREHAALEPRHLGGRVIIVKSFARIHETNLKK 189
D+P +K G PW VI + NYGEGS+REHAAL PR LGG +++VKSFARIHETNLKK
Sbjct: 644 DIPGLMMKWKSDGRPWTVIAEHNYGEGSAREHAALSPRFLGGEILLVKSFARIHETNLKK 703
Query: 190 QGMLPLTFSKPEDYNKIQPSDKISLLNLAKFAPEK-----PVECEIKHKDGAKEVIILNH 354
QG+LPLTF+ DY+KI D + LNL + ++ +I +G I H
Sbjct: 704 QGVLPLTFANESDYDKISSGDVLETLNLVDMIAKDGNNGGEIDVKITKPNGESFTIKAKH 763
Query: 355 TFNKNQIDWFKAGSALN 405
T +K+QID+FKAGSA+N
Sbjct: 764 TMSKDQIDFFKAGSAIN 780
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,625,586
Number of Sequences: 369166
Number of extensions: 1153927
Number of successful extensions: 3938
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3836
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3933
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3783975580
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)