Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_B22
(874 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP... 306 4e-83
sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cy... 303 4e-82
sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cy... 301 2e-81
sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cy... 297 2e-80
sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cy... 296 4e-80
sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cyto... 295 9e-80
sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cy... 291 2e-78
sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mi... 288 2e-77
sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mi... 286 7e-77
sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP... 261 1e-69
>sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 647
Score = 306 bits (785), Expect = 4e-83
Identities = 142/240 (59%), Positives = 180/240 (75%)
Frame = -2
Query: 852 VTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIFGGRRPEG 673
V ITDW G PW + G AAHPNSRFC+PA+QCPIIDE WE GVPISA++FGGRRP G
Sbjct: 407 VQITDWLGKPWTKDSGKPAAHPNSRFCTPAAQCPIIDEAWEDPAGVPISAMLFGGRRPAG 466
Query: 672 VPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDYLKHWIHF 493
VPLIYE+RDW HGVF+GA MRSE+TAAAEHK VIMHDPFAMRPFFGYNFGDY+ HW+
Sbjct: 467 VPLIYEARDWTHGVFIGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGDYVAHWLSM 526
Query: 492 ADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKESAFGNLP 313
+K ++P+I+HVNWFRKSA+ GKF+WPG+GEN RVLEWIL+R++G +SA G++P
Sbjct: 527 --EKRGQVPKIFHVNWFRKSAE-GKFMWPGYGENSRVLEWILRRVNGESCYVDSAIGHIP 583
Query: 312 LISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRNELTKLKS 133
+++ G+ D++E+FS+ F +E ++I YF Q+G LP I +L +L S
Sbjct: 584 AEGALNLDGMK-DKVDVKEIFSLPKEFWSQEVKDIRTYFESQVGADLPASIYQQLDELSS 642
>sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 303 bits (776), Expect = 4e-82
Identities = 146/246 (59%), Positives = 170/246 (69%)
Frame = -2
Query: 873 DEIDFNAVTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIF 694
DE + VTIT W+ W G AHPNSRFC+PASQCPIID WES EGVPI IIF
Sbjct: 374 DEPLASGVTITSWKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIF 433
Query: 693 GGRRPEGVPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDY 514
GGRRP GVPL+YE+ W HGVFVGA MRSE+TAAAEHK +IMHDPFAMRPFFGYNFG Y
Sbjct: 434 GGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKY 493
Query: 513 LKHWIHFADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKE 334
L HW+ A + KLP+I+HVNWFRK K GKFLWPGFGEN RVLEW+ RIDG K
Sbjct: 494 LAHWLSMAQHPAAKLPKIFHVNWFRKD-KEGKFLWPGFGENSRVLEWMFNRIDGKASTKL 552
Query: 333 SAFGNLPLISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRN 154
+ G +P +++ G+ N M ELFS+ F +KE E+IEKY +Q+ LP I
Sbjct: 553 TPIGYIPKEDALNLKGLGHIN--MMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIER 610
Query: 153 ELTKLK 136
E+ LK
Sbjct: 611 EILALK 616
>sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 301 bits (770), Expect = 2e-81
Identities = 145/246 (58%), Positives = 170/246 (69%)
Frame = -2
Query: 873 DEIDFNAVTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIF 694
DE + VTIT W+ W G AHPNSRFC+PASQCPIID WES EGVPI IIF
Sbjct: 374 DEPLASGVTITSWKNKEWSPEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIF 433
Query: 693 GGRRPEGVPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDY 514
GGRRP GVPL+YE+ W HGVFVGA MRSE+TAAAEHK +IMHDPFAMRPFFGYNFG Y
Sbjct: 434 GGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKY 493
Query: 513 LKHWIHFADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKE 334
L HW+ A + KLP+I+HVNWFRK K GKFLWPGFGEN RVLEW+ RIDG K
Sbjct: 494 LAHWLSMAQHPAAKLPKIFHVNWFRKD-KEGKFLWPGFGENSRVLEWMFNRIDGKAGAKL 552
Query: 333 SAFGNLPLISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRN 154
+ G +P +++ G+ N + ELFS+ F +KE E+IEKY +Q+ LP I
Sbjct: 553 TPIGYIPKEDALNLKGLGHIN--VMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIER 610
Query: 153 ELTKLK 136
E+ LK
Sbjct: 611 EILALK 616
>sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 297 bits (761), Expect = 2e-80
Identities = 142/239 (59%), Positives = 168/239 (70%)
Frame = -2
Query: 852 VTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIFGGRRPEG 673
VTIT W+ W AHPNSRFC+PASQCPIID WES EGVPI IIFGGRRPEG
Sbjct: 381 VTITSWKNKEWRPQDAEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPEG 440
Query: 672 VPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDYLKHWIHF 493
VPL+YE+ W HGVFVGA MRSE+TAAAEHK +IMHDPFAMRPFFGYNFG YL HW+
Sbjct: 441 VPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSM 500
Query: 492 ADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKESAFGNLP 313
A + KLP+I+HVNWFRK K GKFLWPGFGEN RVLEW+ RI+G K + G +P
Sbjct: 501 AHRPAAKLPKIFHVNWFRKD-KDGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYIP 559
Query: 312 LISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRNELTKLK 136
+ +++ G+ N +EELF + F +KE EEI++Y +Q+ LP I EL LK
Sbjct: 560 KENALNLKGLGGVN--VEELFGISKEFWEKEVEEIDRYLEDQVNTDLPYEIERELRALK 616
>sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 296 bits (759), Expect = 4e-80
Identities = 138/239 (57%), Positives = 170/239 (71%)
Frame = -2
Query: 852 VTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIFGGRRPEG 673
VT+T W+ W G AHPNSRFC+PASQCPI+D WES EGVPI IIFGGRRP G
Sbjct: 381 VTLTSWKNKDWTPDNGEPCAHPNSRFCTPASQCPIMDPAWESPEGVPIEGIIFGGRRPAG 440
Query: 672 VPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDYLKHWIHF 493
VPL+YE+ +W HGVF+GA MRSE+TAAAEHK +IMHDPFAMRPFFGYNFG YL HW+
Sbjct: 441 VPLVYEAFNWQHGVFIGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSM 500
Query: 492 ADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKESAFGNLP 313
A + KLPRI+HVNWFRK ++ GKFLWPG+GEN RVLEW+ RI G K +A G +P
Sbjct: 501 AHRPAAKLPRIFHVNWFRKDSQ-GKFLWPGYGENSRVLEWMFNRIQGKASAKSTAIGYIP 559
Query: 312 LISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRNELTKLK 136
+ +++ G+ N + ELF++ F +KE EEI++YF Q+ LP I EL L+
Sbjct: 560 ADTALNLKGLEDIN--LTELFNISKEFWEKEVEEIKQYFEGQVNADLPYEIERELLALE 616
>sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 295 bits (756), Expect = 9e-80
Identities = 143/239 (59%), Positives = 166/239 (69%)
Frame = -2
Query: 852 VTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIFGGRRPEG 673
VTIT W+ W AHPNSRFC+PASQCPIID WES EGVPI IIFGGRRP G
Sbjct: 381 VTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAG 440
Query: 672 VPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDYLKHWIHF 493
VPL+YE+ W HGVFVGA MRSE+TAAAEHK VIMHDPFAMRPFFGYNFG YL HW+
Sbjct: 441 VPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGKYLAHWLSM 500
Query: 492 ADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKESAFGNLP 313
A + KLP+I+HVNWFRK K GKFLWPGFGEN RVLEW+ RI+G K + G +P
Sbjct: 501 AHRPAAKLPKIFHVNWFRKD-KNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVP 559
Query: 312 LISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRNELTKLK 136
+++ G+ N +EELF + F +KE EEI+KY +Q+ LP I EL LK
Sbjct: 560 KEDALNLKGLGDVN--VEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALK 616
>sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 291 bits (745), Expect = 2e-78
Identities = 137/239 (57%), Positives = 168/239 (70%)
Frame = -2
Query: 852 VTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIFGGRRPEG 673
V + W+G WD G AHPNSRFC+PASQCPIID +WES EGVPI IIFGGRRP G
Sbjct: 381 VKLISWKGKEWDPKDGEPCAHPNSRFCTPASQCPIIDPDWESPEGVPIEGIIFGGRRPVG 440
Query: 672 VPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDYLKHWIHF 493
VPL+YE+ W HGVFVGA MRSE+TAAAE+K VIMHDPFAM PFFGYNFG YL HW+
Sbjct: 441 VPLVYEALSWQHGVFVGAAMRSEATAAAEYKGKVIMHDPFAMGPFFGYNFGQYLAHWLSM 500
Query: 492 ADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKESAFGNLP 313
A + KLP+I+HVNWFRK K G+FLWPGFGEN RVLEW+ R+ G + G +P
Sbjct: 501 AQRPAAKLPKIFHVNWFRKD-KAGRFLWPGFGENSRVLEWMFNRVVGEGGASVTPIGYIP 559
Query: 312 LISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRNELTKLK 136
+D+ G+ + D++ELF + F ++E EEI+KY EQ+ LP I+N++ LK
Sbjct: 560 DEDALDLRGL--GDVDVKELFHISKEFWEEEVEEIQKYLEEQVNVDLPPEIKNQVLALK 616
>sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 288 bits (736), Expect = 2e-77
Identities = 136/244 (55%), Positives = 170/244 (69%)
Frame = -2
Query: 852 VTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIFGGRRPEG 673
VTIT W G PW AHPNSRFC PA QCPI+D WE+ EGVPI AIIFGGRRP+G
Sbjct: 399 VTITSWLGKPWKPGDKEPCAHPNSRFCVPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKG 458
Query: 672 VPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDYLKHWIHF 493
VPL+YE+ +W HGVFVG+ MRSESTAAAEHK IMHDPFAMRPFFGYNFG YL+HW+
Sbjct: 459 VPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKTIMHDPFAMRPFFGYNFGRYLEHWLSM 518
Query: 492 ADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKESAFGNLP 313
K +LPRI+HVNWFR+ + G+FLWPGFGEN RVL+WI +R++G +E+ G +P
Sbjct: 519 EGQKGARLPRIFHVNWFRRD-EAGRFLWPGFGENARVLDWICRRLEGEDSAQETPIGLVP 577
Query: 312 LISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRNELTKLKS 133
+D+SG+ S D +LFS+ F ++E +I Y EQ+ + LP+ + EL L+
Sbjct: 578 KEGALDLSGL--SAVDTSQLFSIPKDFWEQEVRDIRGYLTEQVNQDLPKEVLAELEALEG 635
Query: 132 HA*K 121
K
Sbjct: 636 RVQK 639
>sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 286 bits (731), Expect = 7e-77
Identities = 133/239 (55%), Positives = 168/239 (70%)
Frame = -2
Query: 852 VTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIFGGRRPEG 673
VT+T W G PW AHPNSRFC+PA QCPI+D WE+ EGVPI AIIFGGRRP+G
Sbjct: 399 VTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKG 458
Query: 672 VPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDYLKHWIHF 493
VPL+YE+ +W HGVFVG MRSESTAAAEHK +IMHDPFAMRPFFGYNFG YL+HW+
Sbjct: 459 VPLVYEAFNWRHGVFVGRAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSM 518
Query: 492 ADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKCKESAFGNLP 313
K +LPRI+HVNWFR+ + G FLWPGFGEN RVL+WI +R++G +E+ G +P
Sbjct: 519 EGRKGAQLPRIFHVNWFRRD-EAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVP 577
Query: 312 LISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIRNELTKLK 136
+D+SG+ D +LFS+ F ++E +I Y EQ+ + LP+ + EL L+
Sbjct: 578 KEGALDLSGLRA--IDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALE 634
>sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 619
Score = 261 bits (668), Expect = 1e-69
Identities = 126/243 (51%), Positives = 164/243 (67%), Gaps = 1/243 (0%)
Frame = -2
Query: 873 DEIDFNAVTITDWQGHPWDRTKGTKAAHPNSRFCSPASQCPIIDENWESLEGVPISAIIF 694
DEI V + +W G W AHPNSRF +PASQCPII WES +GVPI AIIF
Sbjct: 370 DEIKDKNVDMINWLGEKWRIGDPGLCAHPNSRFAAPASQCPIIHPEWESPKGVPIDAIIF 429
Query: 693 GGRRPEGVPLIYESRDWIHGVFVGATMRSESTAAAEHKANVIMHDPFAMRPFFGYNFGDY 514
GGRRP GVPL++E+R W+HG+F GA ++SE+TAAAEHK +MHDP AMRPF GYNFG Y
Sbjct: 430 GGRRPAGVPLVFETRSWLHGIFTGACLKSEATAAAEHKGKTVMHDPMAMRPFMGYNFGHY 489
Query: 513 LKHWIHFADDKSHKLPRIYHVNWFRKSAKTGKFLWPGFGENCRVLEWILKRIDGSVKC-K 337
L+HWI D K+P+IYHVNWFR+ A KFLWPG+G+N RV++WI++R+DG
Sbjct: 490 LQHWIDLNKD-GRKVPKIYHVNWFRRDA-NNKFLWPGYGQNIRVIDWIVRRLDGEPDIGV 547
Query: 336 ESAFGNLPLISDIDVSGINVSNSDMEELFSVDPVFLKKEAEEIEKYFHEQIGKYLPEPIR 157
++ G +P I+ SG+ + +EL SV + +A+EI K+ EQ+G LP+ IR
Sbjct: 548 DTPIGIVPKKGAINASGL--PDIQWDELMSVPKEYWTNDAKEIRKFLEEQVGPDLPKEIR 605
Query: 156 NEL 148
E+
Sbjct: 606 AEM 608
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 102,648,191
Number of Sequences: 369166
Number of extensions: 2201948
Number of successful extensions: 5923
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5655
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5817
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)