Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_A14
(603 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07954|FUMH_HUMAN Fumarate hydratase, mitochondrial prec... 237 2e-62
sp|P97807|FUMH_MOUSE Fumarate hydratase, mitochondrial prec... 237 2e-62
sp|Q60HF9|FUMH_MACFA Fumarate hydratase, mitochondrial prec... 237 2e-62
sp|P14408|FUMH_RAT Fumarate hydratase, mitochondrial precur... 235 6e-62
sp|P10173|FUMH_PIG Fumarate hydratase, mitochondrial (Fumar... 233 4e-61
sp|Q7SX99|FUMH_BRARE Fumarate hydratase, mitochondrial prec... 231 1e-60
sp|P55250|FUMH_RHIOR Fumarate hydratase, mitochondrial prec... 229 3e-60
sp|P93033|FUM1_ARATH Fumarate hydratase 1, mitochondrial pr... 226 3e-59
sp|Q9FI53|FUM2_ARATH Fumarate hydratase 2, chloroplast prec... 225 8e-59
sp|O69294|FUMC_CAMJE Fumarate hydratase class II (Fumarase C) 222 5e-58
>sp|P07954|FUMH_HUMAN Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 510
Score = 237 bits (604), Expect = 2e-62
Identities = 110/174 (63%), Positives = 141/174 (81%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA+++
Sbjct: 337 KIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTV 396
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
G S G+ ELNV+KP++I N+LHS +LL D S F + CV+G+ NT +IN +N+SLMLV
Sbjct: 397 GGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLV 456
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISPK 524
TALN HIGYDK+A+IAK AHKN +LKE A++L ++ E FD+W+ P M+ PK
Sbjct: 457 TALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK 510
>sp|P97807|FUMH_MOUSE Fumarate hydratase, mitochondrial precursor (Fumarase) (EF-3)
Length = 507
Score = 237 bits (604), Expect = 2e-62
Identities = 110/174 (63%), Positives = 141/174 (81%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA+++
Sbjct: 334 KIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTV 393
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
G S G+ ELNV+KP++I N+LHS +LL D S F + CV+G+ NT +IN +N+SLMLV
Sbjct: 394 GGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLV 453
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISPK 524
TALN HIGYDK+A+IAK AHKN +LKE A++L ++ E FD+W+ P M+ PK
Sbjct: 454 TALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK 507
>sp|Q60HF9|FUMH_MACFA Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 510
Score = 237 bits (604), Expect = 2e-62
Identities = 110/174 (63%), Positives = 141/174 (81%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA+++
Sbjct: 337 KIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTV 396
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
G S G+ ELNV+KP++I N+LHS +LL D S F + CV+G+ NT +IN +N+SLMLV
Sbjct: 397 GGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLV 456
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISPK 524
TALN HIGYDK+A+IAK AHKN +LKE A++L ++ E FD+W+ P M+ PK
Sbjct: 457 TALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK 510
>sp|P14408|FUMH_RAT Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 507
Score = 235 bits (600), Expect = 6e-62
Identities = 109/174 (62%), Positives = 141/174 (81%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA+++
Sbjct: 334 KIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTV 393
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
G S G+ ELNV+KP++I N+LHS +LL D S F + CV+G+ NT +IN +N+SLMLV
Sbjct: 394 GGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLV 453
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISPK 524
TALN HIGYDK+A+IAK AHKN +LK+ A++L ++ E FD+W+ P M+ PK
Sbjct: 454 TALNPHIGYDKAAKIAKTAHKNGSTLKKTAIELGYLTAEQFDEWVKPKDMLGPK 507
>sp|P10173|FUMH_PIG Fumarate hydratase, mitochondrial (Fumarase)
Length = 466
Score = 233 bits (593), Expect = 4e-61
Identities = 108/174 (62%), Positives = 140/174 (80%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+++MV AQVMGN+VA+++
Sbjct: 293 KIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEALTMVAAQVMGNHVAVTV 352
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
G S G+ ELNV+KP++I N+LHS +LL D + F + CV+G+ NT +IN +N+SLMLV
Sbjct: 353 GGSNGHFELNVFKPMMIKNVLHSARLLGDAAVSFTENCVVGIQANTERINKLMNESLMLV 412
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISPK 524
TALN HIGYDK+A+IAK AHKN +LK AV+L ++ E FD+W+ P M+ PK
Sbjct: 413 TALNPHIGYDKAAKIAKTAHKNGSTLKATAVELGYLTAEQFDEWVKPRDMLGPK 466
>sp|Q7SX99|FUMH_BRARE Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 509
Score = 231 bits (588), Expect = 1e-60
Identities = 107/174 (61%), Positives = 142/174 (81%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA+++
Sbjct: 336 KIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTV 395
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
G S G+ ELNV+KP+II N+L+S +LL D S F CV+G+ NT +IN +++SLMLV
Sbjct: 396 GGSNGHFELNVFKPMIIKNVLNSARLLGDASVSFTNNCVVGIEANTERINKLMSESLMLV 455
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISPK 524
TALN HIGYDK+A+IAK AHK+ +LKEAA++L ++++ F++W+ P M+ PK
Sbjct: 456 TALNPHIGYDKAAKIAKTAHKDGSTLKEAALKLGFLNEQQFEEWVRPHDMLGPK 509
>sp|P55250|FUMH_RHIOR Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 494
Score = 229 bits (585), Expect = 3e-60
Identities = 109/174 (62%), Positives = 138/174 (79%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND R+LGSGPRCG+GE LPENEPGSSIMPGK+NPTQCE+M+MVCAQVMGN AIS+
Sbjct: 320 KIANDIRYLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEAMTMVCAQVMGNNTAISV 379
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
S G ELNV+KP++I NL+ SI+L++D S F K CV+G+ N +I++ +N+SLMLV
Sbjct: 380 AGSNGQFELNVFKPVMIKNLIQSIRLISDASISFTKNCVVGIEANEKKISSIMNESLMLV 439
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISPK 524
TALN HIGYDK+A+ AK AHK +LKEAA+ L ++ E+FD+W+ P MIS K
Sbjct: 440 TALNPHIGYDKAAKCAKKAHKEGTTLKEAALSLGYLTSEEFDQWVRPEDMISAK 493
>sp|P93033|FUM1_ARATH Fumarate hydratase 1, mitochondrial precursor (Fumarase 1)
Length = 492
Score = 226 bits (576), Expect = 3e-59
Identities = 106/173 (61%), Positives = 139/173 (80%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND RFLGSGPRCG+GE +LPENEPGSSIMPGK+NPTQCE+++MVCAQVMGN+VA+++
Sbjct: 318 KIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTV 377
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
G S G+ ELNV+KP+I + LLHS++L+AD S F K CV G+ N +I+ +++SLMLV
Sbjct: 378 GGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIEANRERISKLLHESLMLV 437
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISP 521
T+LN IGYD +A +AK AHK C+LKEAA+ L +++ E+FD + P KMI P
Sbjct: 438 TSLNPKIGYDNAAAVAKKAHKEGCTLKEAALNLGVLTAEEFDTLVVPEKMIGP 490
>sp|Q9FI53|FUM2_ARATH Fumarate hydratase 2, chloroplast precursor (Fumarase 2)
Length = 499
Score = 225 bits (573), Expect = 8e-59
Identities = 107/173 (61%), Positives = 138/173 (79%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND RFLGSGPRCG+GE LPENEPGSSIMPGK+NPTQCE+++MVCAQVMGN+VA++I
Sbjct: 325 KIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTI 384
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
G S G+ ELNV+KP+I + LLHSI+L+AD S F K CV G+ N +I+ +++SLMLV
Sbjct: 385 GGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESLMLV 444
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISP 521
T+LN IGYD +A +AK AHK C+LK AA++L +++ E+FD + P KMI P
Sbjct: 445 TSLNPKIGYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGP 497
>sp|O69294|FUMC_CAMJE Fumarate hydratase class II (Fumarase C)
Length = 463
Score = 222 bits (566), Expect = 5e-58
Identities = 107/174 (61%), Positives = 137/174 (78%)
Frame = +3
Query: 3 KIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVAISI 182
KIAND R+L SGPRCG+GE ++PENEPGSSIMPGK+NPTQCE+++MV QVMGN VAI
Sbjct: 289 KIANDIRWLASGPRCGLGELIIPENEPGSSIMPGKVNPTQCEAVTMVAVQVMGNDVAIGF 348
Query: 183 GSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSLMLV 362
+SQGN ELNV+KP+II N L S+ LLAD F +C +G+ N A+I+ ++ SLMLV
Sbjct: 349 AASQGNFELNVFKPVIIYNFLQSLDLLADSMHSFNIHCAVGIEPNRAKIDHNLHNSLMLV 408
Query: 363 TALNSHIGYDKSAQIAKYAHKNNCSLKEAAVQLKLVSDEDFDKWLDPWKMISPK 524
TALN HIGY+ +A++AK AHK SLKE+ ++L LVS+EDF+K++DP KMI PK
Sbjct: 409 TALNPHIGYENAAKVAKNAHKKGISLKESTMELGLVSEEDFNKFVDPTKMIGPK 462
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,331,522
Number of Sequences: 369166
Number of extensions: 1373795
Number of successful extensions: 3772
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3681
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3766
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4650991475
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)