Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_P15
(915 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O00418|EF2K_HUMAN Elongation factor 2 kinase (eEF-2 kina... 135 2e-31
sp|P70531|EF2K_RAT Elongation factor 2 kinase (eEF-2 kinase... 131 3e-30
sp|O08796|EF2K_MOUSE Elongation factor 2 kinase (eEF-2 kina... 130 4e-30
sp|O01991|EF2K_CAEEL Elongation factor 2 kinase (eEF-2 kina... 93 1e-18
sp|O00763|COA2_HUMAN Acetyl-CoA carboxylase 2 (ACC-beta) [I... 33 1.5
sp|Q9UBV2|SEL1L_HUMAN Sel-1 homolog precursor (Suppressor o... 31 5.5
sp|Q9NP71|WBS14_HUMAN Williams-Beuren syndrome chromosome r... 31 5.5
sp|Q9DCV6|K0141_MOUSE Protein KIAA0141 31 5.5
sp|Q9Z2G6|SEL1L_MOUSE Sel-1 homolog precursor (Suppressor o... 31 5.5
sp|Q80Z70|SEL1L_RAT Sel-1 homolog precursor (Suppressor of ... 31 5.5
>sp|O00418|EF2K_HUMAN Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 725
Score = 135 bits (339), Expect = 2e-31
Identities = 88/261 (33%), Positives = 130/261 (49%)
Frame = +3
Query: 120 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 299
+L+++IG SIL +VH + R HE GRF C + W
Sbjct: 513 DLEKKIGKSILGKVHLAMVRYHEGGRF---------CEKGEEWDQ--------------- 548
Query: 300 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 479
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 549 ------ESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLL 602
Query: 480 RAADSGDRRSMIELANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAANKPD 659
+AA++GDR+SMI +A + +N + +W EA+ WY A E + +
Sbjct: 603 KAAEAGDRQSMILVARAFDSGQNLSPDRCQ----------DWLEALHWYNTALEMTDCDE 652
Query: 660 GQLDSKDAEGRYDSADDLMPPQEILAAMAELHFKGGYGLERNCQLAGELYSQAAEIATNA 839
G G YD D P +LA AE+ F GGYGLE++ Q +G+LY+QAAE A A
Sbjct: 653 G--------GEYDGMQD-EPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEA 703
Query: 840 LQGRQAVKYYELSDIAYSQDE 902
++GR A +YY+ ++ A++Q E
Sbjct: 704 MKGRLANQYYQKAEEAWAQME 724
>sp|P70531|EF2K_RAT Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 724
Score = 131 bits (329), Expect = 3e-30
Identities = 86/261 (32%), Positives = 127/261 (48%)
Frame = +3
Query: 120 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 299
+L ++IG S+L +VH + R HE GRF C + W
Sbjct: 512 DLGRKIGKSVLGKVHLAMVRYHEGGRF---------CEKDEEWDQ--------------- 547
Query: 300 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 479
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 548 ------ESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLEETEENKTKGFDYLL 601
Query: 480 RAADSGDRRSMIELANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAANKPD 659
+AA++GDR+SMI +A + N + +W EA+ WY A E + +
Sbjct: 602 KAAEAGDRQSMILVARAFDTGLNLSPDRCQ----------DWSEALHWYNTALETTDCDE 651
Query: 660 GQLDSKDAEGRYDSADDLMPPQEILAAMAELHFKGGYGLERNCQLAGELYSQAAEIATNA 839
G G YD D P +LA AE+ GG+GL++N Q +G+LY+QAAE A A
Sbjct: 652 G--------GEYDGIQD-EPQYALLAREAEMLLTGGFGLDKNPQRSGDLYTQAAEAAMEA 702
Query: 840 LQGRQAVKYYELSDIAYSQDE 902
++GR A +YYE ++ A++Q E
Sbjct: 703 MKGRLANQYYEKAEEAWAQME 723
>sp|O08796|EF2K_MOUSE Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 724
Score = 130 bits (328), Expect = 4e-30
Identities = 86/261 (32%), Positives = 126/261 (48%)
Frame = +3
Query: 120 NLDQEIGHSILAQVHHELARLHESGRFIKSKQLAAGCLHHQFWSHLLNENDSPEAIVKET 299
+L ++IG S+L +VH + R HE GRF C + W
Sbjct: 512 DLGRKIGKSVLGKVHLAMVRYHEGGRF---------CEKDEEWDR--------------- 547
Query: 300 SNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHMLQDCPFPPTQNDLDCGLDYLW 479
ES +FH HAA LG LEA++ + + H+L D T+ + G DYL
Sbjct: 548 ------ESAIFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLL 601
Query: 480 RAADSGDRRSMIELANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAANKPD 659
+AA++GDR SMI +A + N + +W EA+ WY A E + +
Sbjct: 602 KAAEAGDRHSMILVARAFDTGLNLSPDRCQ----------DWSEALHWYNTALETTDCDE 651
Query: 660 GQLDSKDAEGRYDSADDLMPPQEILAAMAELHFKGGYGLERNCQLAGELYSQAAEIATNA 839
G G YD D P +LA AE+ GG+GL++N Q +G+LY+QAAE A A
Sbjct: 652 G--------GEYDGIQD-EPQYALLAREAEMLLTGGFGLDKNPQRSGDLYTQAAEAAMEA 702
Query: 840 LQGRQAVKYYELSDIAYSQDE 902
++GR A +YYE ++ A++Q E
Sbjct: 703 MKGRLANQYYEKAEEAWAQME 723
>sp|O01991|EF2K_CAEEL Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K)
(Calcium/calmodulin-dependent eukaryotic elongation
factor 2 kinase)
Length = 768
Score = 92.8 bits (229), Expect = 1e-18
Identities = 81/296 (27%), Positives = 144/296 (48%), Gaps = 26/296 (8%)
Frame = +3
Query: 75 SMRENQKPSSATHPTNLDQEIGH--SILAQVHHELARLHESGRFIK----SKQLAAGCLH 236
S++ Q ++ + ++ Q GH S+L Q+H +L+R HE GRF++ K++ G
Sbjct: 500 SLQLQQMAANLENDEDVPQVTGHQFSVLGQIHIDLSRYHELGRFVEVDSEHKEMLEG--- 556
Query: 237 HQFWSHLLNENDSPEAIVKETSNDINWESCLFHERHAAQLGCLEAVIVMARYCLNMSGHM 416
+END+ I + +S +FH A + G LEAV+ A L + +
Sbjct: 557 --------SENDARVPIKYDK------QSAIFHLDIARKCGILEAVLTSAHIVLGLPHEL 602
Query: 417 LQDCP--------FPPTQNDL------------DCGLDYLWRAADSGDRRSMIELANLYR 536
L++ F +N + + G D + AA+ GD+ +M+ +A+ Y
Sbjct: 603 LKEVTVDDLFPNGFGEQENGIRADKGQKPCDLEEFGSDLMEIAAEMGDKGAMLYMAHAY- 661
Query: 537 KTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAANKPDGQLDSKDAEGRYDSADDLM 716
E + G +T ++ +++ WY+R + + +LDS + + S L
Sbjct: 662 -----ETGQHLGPNRRT----DYKKSIDWYQRV--VGFQEEEELDSDCGKTTFSSFAPLT 710
Query: 717 PPQEILAAMAELHFKGGYGLERNCQLAGELYSQAAEIATNALQGRQAVKYYELSDI 884
EILA MAE++ +GGYGL ++ + A L+++AAE A A+ G+ A KYYE +++
Sbjct: 711 R-HEILAKMAEMYKEGGYGLNQDFERAYGLFNEAAEAAMEAMNGKLANKYYEKAEM 765
>sp|O00763|COA2_HUMAN Acetyl-CoA carboxylase 2 (ACC-beta) [Includes: Biotin carboxylase ]
Length = 2483
Score = 32.7 bits (73), Expect = 1.5
Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 3/56 (5%)
Frame = +3
Query: 624 YKRAFEAANKPD-GQLDSKDAEGRYDSADDLMPP--QEILAAMAELHFKGGYGLER 782
YK+ E +PD D KD EGR + +DL+ P ++ A+ H G LE+
Sbjct: 2280 YKKLMEQLGEPDLSDKDRKDLEGRLKAREDLLLPIYHQVAVQFADFHDTPGRMLEK 2335
>sp|Q9UBV2|SEL1L_HUMAN Sel-1 homolog precursor (Suppressor of lin-12-like protein)
(Sel-1L)
Length = 794
Score = 30.8 bits (68), Expect = 5.5
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = +3
Query: 741 MAELHFKGGYGLERNCQLAGELYSQAAEIATNALQGRQAVKYYELSDIAYSQDE 902
+ +LH GG G+E+N Q A + ++ AA + Y E SDI +E
Sbjct: 379 LGQLHLHGGRGVEQNHQRAFDYFNLAANAGNSHAMAFLGKMYSEGSDIVPQSNE 432
>sp|Q9NP71|WBS14_HUMAN Williams-Beuren syndrome chromosome region 14 protein (WS
basic-helix-loop-helix leucine zipper protein) (WS-bHLH)
(Mlx interactor) (MLX-interacting protein-like)
Length = 852
Score = 30.8 bits (68), Expect = 5.5
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 3/61 (4%)
Frame = +3
Query: 522 ANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKW---YKRAFEAANKPDGQLDSKDAEGR 692
A+ +RK E EGN K ++V E KW YK+ ++ D L K AEGR
Sbjct: 154 ADAHRKPEAV---VLEGNYWKRRIEVVMREYHKWRIYYKKRLRKPSREDDLLAPKQAEGR 210
Query: 693 Y 695
+
Sbjct: 211 W 211
>sp|Q9DCV6|K0141_MOUSE Protein KIAA0141
Length = 510
Score = 30.8 bits (68), Expect = 5.5
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +3
Query: 471 YLWRAADSGDRRSMIELANLYRKTENTEEKTYEGNIMKTLLDVNWYEAVKWYKRAFEAAN 650
YLW AA +GD +S L Y K + N EAVK Y++A N
Sbjct: 376 YLWLAASNGDSQSRFHLGICYEKGLGAQR--------------NLGEAVKCYQQAAAMGN 421
Query: 651 KP 656
+P
Sbjct: 422 EP 423
>sp|Q9Z2G6|SEL1L_MOUSE Sel-1 homolog precursor (Suppressor of lin-12-like protein)
(Sel-1L)
Length = 790
Score = 30.8 bits (68), Expect = 5.5
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = +3
Query: 741 MAELHFKGGYGLERNCQLAGELYSQAAEIATNALQGRQAVKYYELSDIAYSQDE 902
+ +LH GG G+E+N Q A + ++ AA + Y E SDI +E
Sbjct: 375 LGQLHLHGGRGVEQNHQRAFDYFNLAANAGNSHAMAFLGKMYSEGSDIVPQSNE 428
>sp|Q80Z70|SEL1L_RAT Sel-1 homolog precursor (Suppressor of lin-12-like protein)
(Sel-1L)
Length = 794
Score = 30.8 bits (68), Expect = 5.5
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = +3
Query: 741 MAELHFKGGYGLERNCQLAGELYSQAAEIATNALQGRQAVKYYELSDIAYSQDE 902
+ +LH GG G+E+N Q A + ++ AA + Y E SDI +E
Sbjct: 379 LGQLHLHGGRGVEQNHQRAFDYFNLAANAGNSHAMAFLGKMYSEGSDIVPQSNE 432
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,048,516
Number of Sequences: 369166
Number of extensions: 1922727
Number of successful extensions: 5023
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4861
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5016
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9318621220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)