Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_M23
(753 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q10348|YDA6_SCHPO Putative endonuclease C1F12.06c 82 2e-15
sp|P52887|ALG8_CAEEL Probable dolichyl pyrophosphate Glc1Ma... 75 2e-13
sp|Q6HGE0|NFI_BACHK Endonuclease V (Deoxyinosine 3'endonucl... 65 3e-10
sp|P96724|NFI_BACSU Endonuclease V (Deoxyinosine 3'endonucl... 65 3e-10
sp|Q8ZYP2|NFI_PYRAE Endonuclease V (Deoxyinosine 3'endonucl... 64 3e-10
sp|Q97M37|NFI_CLOAB Endonuclease V (Deoxyinosine 3'endonucl... 62 2e-09
sp|O66824|NFI_AQUAE Endonuclease V (Deoxyinosine 3'endonucl... 60 8e-09
sp|Q9X2H9|NFI_THEMA Endonuclease V (Deoxyinosine 3'endonucl... 59 2e-08
sp|Q8YPV5|NFI_ANASP Endonuclease V (Deoxyinosine 3'endonucl... 59 2e-08
sp|Q82MH6|NFI_STRAW Endonuclease V (Deoxyinosine 3'endonucl... 55 2e-07
>sp|Q10348|YDA6_SCHPO Putative endonuclease C1F12.06c
Length = 252
Score = 82.0 bits (201), Expect = 2e-15
Identities = 72/206 (34%), Positives = 104/206 (50%), Gaps = 14/206 (6%)
Frame = +1
Query: 31 IRNMNNPDKSIIEIWKTKQLYLKELLILDNVESWQEELNHKIDEMNDLIIPSNLKLIGGI 210
+ N N DK + IW+ +Q+ LK +L +L K +N++ + + G+
Sbjct: 1 MNNDNLHDK--LTIWRQQQIELKGML----------QLIPKFQSLNEI------RYVVGL 42
Query: 211 DISFDKSNNNLACVCFGVIDSCTKKLVRK-HEKIVSIIEPYIVNYLAFRECEHMVGTIKE 387
DISFDKS+ A + D + ++ K + I + E Y+ +L+FRE IK
Sbjct: 43 DISFDKSSPK-AVSALVIYDLEQRMIIYKDYLCIEKLEEDYVPGFLSFRE-------IKW 94
Query: 388 FLVLYPEMKPQ----VILVDGDGVYHTMGFGSACQLGVRLEQEGIDIPTVGVAKNYLQLH 555
+L L + Q +ILVDG+GV H +GFG AC LGV L ++P VGVAKNYL
Sbjct: 95 YLPLLNHIPHQFRIDIILVDGNGVLHPVGFGLACHLGVLL-----NLPVVGVAKNYLHCV 149
Query: 556 GMPS-----REELK----EKITDHMI 606
G+ RE LK +K TDH I
Sbjct: 150 GLTESLDAHRETLKKHVLKKTTDHPI 175
>sp|P52887|ALG8_CAEEL Probable dolichyl pyrophosphate Glc1Man9GlcNAc2
alpha-1,3-glucosyltransferase
(Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl
glucosyltransferase)
Length = 758
Score = 75.1 bits (183), Expect = 2e-13
Identities = 57/166 (34%), Positives = 81/166 (48%), Gaps = 1/166 (0%)
Frame = +1
Query: 61 IIEIWKTKQL-YLKELLILDNVESWQEELNHKIDEMNDLIIPSNLKLIGGIDISFDKSNN 237
I EIW K ++E LI D+ S Q +++++D+ KL+ GID S K N+
Sbjct: 501 IFEIWWAKATCQIRERLIRDSTYSVQA-----VEDLDDV------KLVAGIDTSAAKLNS 549
Query: 238 NLACVCFGVIDSCTKKLVRKHEKIVSIIEPYIVNYLAFRECEHMVGTIKEFLVLYPEMKP 417
++ + K V + PYI YLA RE E M +K + PE++P
Sbjct: 550 DMVYISVSFWTYPDLKHVATISDTRMLELPYIPQYLAVREAEVMADFLKSVITERPELRP 609
Query: 418 QVILVDGDGVYHTMGFGSACQLGVRLEQEGIDIPTVGVAKNYLQLH 555
VIL DG G +H+ G G AC +G I ++GVAKN L LH
Sbjct: 610 DVILCDGFGEFHSRGCGMACHVGAL-----SGIASIGVAKN-LTLH 649
>sp|Q6HGE0|NFI_BACHK Endonuclease V (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V)
(DNase V)
Length = 235
Score = 64.7 bits (156), Expect = 3e-10
Identities = 50/173 (28%), Positives = 80/173 (46%), Gaps = 3/173 (1%)
Frame = +1
Query: 100 ELLILDNVESWQEELNHKIDEMNDLIIPSNLKLIGGIDISFDKSNNN-LACVCFGVIDSC 276
E ++ Q EL+ +I N+ I ++ I G+D+++ N C +ID
Sbjct: 9 EKKLIQEFSKIQSELSPRIKLENNFQI-EEIQFIAGVDLAYWNVNKTKYGTCCIVIIDYG 67
Query: 277 TKKLVRKHEKIVSIIEPYIVNYLAFRECEHMVGTIKEFLVLYPEMKPQVILVDGDGVYHT 456
TK++V K I PYI +LAFRE ++ +K+ +P + DG+G H
Sbjct: 68 TKEVVEKVYSYGEIPIPYIPGFLAFRELPLIIEAVKKL-----TKQPDIYFFDGNGYLHY 122
Query: 457 MGFGSACQLGVRLEQEGIDIPTVGVAKNYLQLHGMP--SREELKEKITDHMID 609
G A L + PT+GVAK+YL++ + E LK TD +I+
Sbjct: 123 RHMGIATHASFLLNK-----PTIGVAKSYLKIRDVDFIMPENLKGSYTDIVIN 170
>sp|P96724|NFI_BACSU Endonuclease V (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V)
(DNase V)
Length = 238
Score = 64.7 bits (156), Expect = 3e-10
Identities = 48/163 (29%), Positives = 80/163 (49%), Gaps = 3/163 (1%)
Frame = +1
Query: 133 QEELNHKIDEMNDLIIPSNLKLIGGIDISF-DKSNNNLACVCFGVIDSCTKKLVRKHEKI 309
Q L ++I+ ++ I P ++ G+D+++ ++ C VID+ TK+++ K +
Sbjct: 21 QFNLKNRIN-LSPTIHPDSINTGAGVDLAYWEQDGEPYGVCCIIVIDADTKEVIEKVHSM 79
Query: 310 VSIIEPYIVNYLAFRECEHMVGTIKEFLVLYPEMKPQVILVDGDGVYHTMGFGSACQLGV 489
I PY+ +LAFRE ++ K+ E +P V L DG+G H G A
Sbjct: 80 GRISVPYVSGFLAFRELPLIIEAAKKL-----ETEPDVFLFDGNGYLHYNHMGVATHAAF 134
Query: 490 RLEQEGIDIPTVGVAKNYLQLHG--MPSREELKEKITDHMIDG 612
L + PT+G+AK YL++ G + E TD +IDG
Sbjct: 135 FLGK-----PTIGIAKTYLKIKGCDFVTPEIEVGAYTDIIIDG 172
>sp|Q8ZYP2|NFI_PYRAE Endonuclease V (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V)
(DNase V)
Length = 215
Score = 64.3 bits (155), Expect = 3e-10
Identities = 47/136 (34%), Positives = 76/136 (55%)
Frame = +1
Query: 190 LKLIGGIDISFDKSNNNLACVCFGVIDSCTKKLVRKHEKIVSIIEPYIVNYLAFRECEHM 369
++ + G+D+++ S LA V+ T ++V + I+ PY+ +LAFRE M
Sbjct: 28 VETVAGLDVAYSGS---LAFGAAVVVKRTTLEVVETACSVSRIVVPYVPTFLAFRELTPM 84
Query: 370 VGTIKEFLVLYPEMKPQVILVDGDGVYHTMGFGSACQLGVRLEQEGIDIPTVGVAKNYLQ 549
++ ++ L + KP VILVDG GV H FG A +GV L++ PT+GVAK+ +
Sbjct: 85 ---LRAYIKL--KSKPDVILVDGHGVAHPRRFGIASHIGVVLKK-----PTIGVAKS--R 132
Query: 550 LHGMPSREELKEKITD 597
L+G EE+ +K+ D
Sbjct: 133 LYG----EEVGDKLVD 144
>sp|Q97M37|NFI_CLOAB Endonuclease V (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V)
(DNase V)
Length = 234
Score = 61.6 bits (148), Expect = 2e-09
Identities = 38/126 (30%), Positives = 64/126 (50%), Gaps = 1/126 (0%)
Frame = +1
Query: 187 NLKLIGGIDISF-DKSNNNLACVCFGVIDSCTKKLVRKHEKIVSIIEPYIVNYLAFRECE 363
++KL G+D+++ K C VID T +++ K I PY+ +LAFRE
Sbjct: 38 DIKLCAGVDLAYWTKGEKQYGVCCIIVIDYNTGEIIEKAYDYGEIEVPYMPGFLAFRELP 97
Query: 364 HMVGTIKEFLVLYPEMKPQVILVDGDGVYHTMGFGSACQLGVRLEQEGIDIPTVGVAKNY 543
++ T+K+ + +P + + DG+G H G A L + PT+GVAK+Y
Sbjct: 98 LVIKTVKKL-----KNEPDIFMFDGNGYLHYNHMGIATHASFFLNK-----PTIGVAKSY 147
Query: 544 LQLHGM 561
L++ G+
Sbjct: 148 LKVAGV 153
>sp|O66824|NFI_AQUAE Endonuclease V (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V)
(DNase V)
Length = 220
Score = 59.7 bits (143), Expect = 8e-09
Identities = 49/150 (32%), Positives = 73/150 (48%), Gaps = 4/150 (2%)
Frame = +1
Query: 109 ILDNVESWQEELNHKIDEMNDLIIPSNLKLIGGIDISFDKSNNN--LACVCFGVIDSCTK 282
+L+ ++ Q E K+ +D ++ IGG+D++F+K N N A V++ T
Sbjct: 3 LLEKLKKIQLECAKKVIARDDF---EKVETIGGMDLTFEKINENPTRAWASLVVVELKTL 59
Query: 283 KLVRKHEKIVSIIEPYIVNYLAFRECEHMVGTIKEFLVLYP--EMKPQVILVDGDGVYHT 456
K V +H + PYI +LAFRE + L LY ++KP V +DG G+ H
Sbjct: 60 KPVYQHVVKDIVDFPYIPTFLAFREMPLL-------LKLYETAKVKPDVYFIDGQGIAHP 112
Query: 457 MGFGSACQLGVRLEQEGIDIPTVGVAKNYL 546
G G A GV + TVGVAK+ L
Sbjct: 113 RGCGIASHFGVETGE-----VTVGVAKSKL 137
>sp|Q9X2H9|NFI_THEMA Endonuclease V (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V)
(DNase V)
Length = 225
Score = 58.5 bits (140), Expect = 2e-08
Identities = 48/155 (30%), Positives = 78/155 (50%), Gaps = 2/155 (1%)
Frame = +1
Query: 199 IGGIDISFDKSNNNLACVCFGVIDSCTKKLVRKHEKIVSIIEPYIVNYLAFRECEHMVGT 378
+ G+D+SF LA + V++ + K++ + I PYI LAFRE G
Sbjct: 39 VAGVDLSFPGKEEGLAVIV--VLEYPSFKILEVVSERGEITFPYIPGLLAFRE-----GP 91
Query: 379 IKEFLVLYPEM--KPQVILVDGDGVYHTMGFGSACQLGVRLEQEGIDIPTVGVAKNYLQL 552
+ FL + ++ KP V++ DG G+ H G A +G+ +E IPT+GVAK+ +L
Sbjct: 92 L--FLKAWEKLRTKPDVVVFDGQGLAHPRKLGIASHMGLFIE-----IPTIGVAKS--RL 142
Query: 553 HGMPSREELKEKITDHMIDGSEILLDLFLDEESNA 657
+G E K ++ DG EI+ + +E +A
Sbjct: 143 YGTFKMPEDKRCSWSYLYDGEEIIGCVIRTKEGSA 177
>sp|Q8YPV5|NFI_ANASP Endonuclease V (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V)
(DNase V)
Length = 221
Score = 58.5 bits (140), Expect = 2e-08
Identities = 40/147 (27%), Positives = 73/147 (49%)
Frame = +1
Query: 106 LILDNVESWQEELNHKIDEMNDLIIPSNLKLIGGIDISFDKSNNNLACVCFGVIDSCTKK 285
L ++ + QEEL H++ + P ++ + G+D+ F+ ++ ++ V+ +
Sbjct: 11 LTVEEAITIQEELRHQVITEDQFTQP--VQYVAGVDMGFE-ADGTISRAAVAVLSFPDLQ 67
Query: 286 LVRKHEKIVSIIEPYIVNYLAFRECEHMVGTIKEFLVLYPEMKPQVILVDGDGVYHTMGF 465
++ + PYI +L+FRE ++ + + + KP +IL DG G+ H
Sbjct: 68 VIETNLAYRPTSFPYIPGFLSFREIPAVLDALAKV-----QTKPDIILCDGQGIAHPRRL 122
Query: 466 GSACQLGVRLEQEGIDIPTVGVAKNYL 546
G A LGV L +IPT+GVAK+ L
Sbjct: 123 GIASHLGVLL-----NIPTIGVAKSLL 144
>sp|Q82MH6|NFI_STRAW Endonuclease V (Deoxyinosine 3'endonuclease) (Deoxyribonuclease V)
(DNase V)
Length = 229
Score = 55.5 bits (132), Expect = 2e-07
Identities = 43/142 (30%), Positives = 66/142 (46%), Gaps = 6/142 (4%)
Frame = +1
Query: 133 QEELNHKIDEMNDLII------PSNLKLIGGIDISFDKSNNNLACVCFGVIDSCTKKLVR 294
+EE DE+ +I P + G+D+++D + + V+D+ T +V
Sbjct: 14 EEEARAVQDELRGRVILDEPGPPPGTGRVTGVDVAYDDERDVVVAAAV-VLDAATLDVVA 72
Query: 295 KHEKIVSIIEPYIVNYLAFRECEHMVGTIKEFLVLYPEMKPQVILVDGDGVYHTMGFGSA 474
+ + + PY+ LAFRE + T+ L P P +I+ DG GV H FG A
Sbjct: 73 EATAVGEVSFPYVPGLLAFRE----IPTVLAALDALP-CPPGLIVCDGYGVAHPRRFGLA 127
Query: 475 CQLGVRLEQEGIDIPTVGVAKN 540
LGV +PT+GVAKN
Sbjct: 128 SHLGVL-----TGLPTIGVAKN 144
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,095,929
Number of Sequences: 369166
Number of extensions: 1831044
Number of successful extensions: 5511
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5309
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5475
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6873311200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)