Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_K18
(362 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--pr... 149 3e-36
sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--pr... 149 3e-36
sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--pr... 147 6e-36
sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--pr... 147 8e-36
sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--pr... 123 1e-28
sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosacch... 92 5e-19
sp|P09147|GALE_ECOLI UDP-glucose 4-epimerase (Galactowalden... 29 3.0
sp|Q56093|GALE_SALTI UDP-glucose 4-epimerase (Galactowalden... 29 3.0
sp|Q8XJP7|TRMD_CLOPE tRNA (Guanine-N(1)-)-methyltransferase... 29 3.9
sp|Q9FI00|ML11_ARATH MLO-like protein 11 (AtMlo11) 28 5.1
>sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 439
Score = 149 bits (375), Expect = 3e-36
Identities = 74/121 (61%), Positives = 92/121 (76%), Gaps = 1/121 (0%)
Frame = +2
Query: 2 FDEEKTAVIDHPIFDRKDDGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQN 178
FDEEKTAVIDH +D D G+HTLI+AD N+ K P IVGK +P+LF+GVG+ AD N
Sbjct: 132 FDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDN 191
Query: 179 PLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDYL 358
PL++++L SS++YSF PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD
Sbjct: 192 PLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSF 251
Query: 359 F 361
F
Sbjct: 252 F 252
>sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 441
Score = 149 bits (375), Expect = 3e-36
Identities = 74/121 (61%), Positives = 92/121 (76%), Gaps = 1/121 (0%)
Frame = +2
Query: 2 FDEEKTAVIDHPIFDRKDDGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQN 178
FDEEKTAVIDH +D D G+HTLI+AD N+ K P IVGK +P+LF+GVG+ AD N
Sbjct: 134 FDEEKTAVIDHHNYDVSDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDN 193
Query: 179 PLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDYL 358
PL++++L SS++YSF PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD
Sbjct: 194 PLVLDILTGSSTSYSFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAF 253
Query: 359 F 361
F
Sbjct: 254 F 254
>sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(OST 50 kDa subunit) (DDOST 48 kDa subunit)
Length = 413
Score = 147 bits (372), Expect = 6e-36
Identities = 73/121 (60%), Positives = 92/121 (76%), Gaps = 1/121 (0%)
Frame = +2
Query: 2 FDEEKTAVIDHPIFDRKDDGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQN 178
FDEE+TAVIDH +D D G+HTLI+AD N+ K P IVGK +P+LF+GVG+ AD N
Sbjct: 106 FDEERTAVIDHHNYDISDPGQHTLIVADAENLLKAPTIVGKKALNPILFRGVGMVADPDN 165
Query: 179 PLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDYL 358
PL++++L SS++YSF PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD
Sbjct: 166 PLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAF 225
Query: 359 F 361
F
Sbjct: 226 F 226
>sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit)
Length = 445
Score = 147 bits (371), Expect = 8e-36
Identities = 73/121 (60%), Positives = 92/121 (76%), Gaps = 1/121 (0%)
Frame = +2
Query: 2 FDEEKTAVIDHPIFDRKDDGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQN 178
FDEEKTAVIDH +D D G+HTLI+AD N+ K P IVG+ +P+LF+GVG+ AD N
Sbjct: 138 FDEEKTAVIDHHNYDVSDLGQHTLIVADPENLLKAPTIVGRSSLNPILFRGVGMVADPDN 197
Query: 179 PLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDYL 358
PL++++L SS++YSF PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD
Sbjct: 198 PLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAF 257
Query: 359 F 361
F
Sbjct: 258 F 258
>sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit) (OST5OP) (DrOST)
(DmOST50)
Length = 449
Score = 123 bits (309), Expect = 1e-28
Identities = 64/124 (51%), Positives = 84/124 (67%), Gaps = 5/124 (4%)
Frame = +2
Query: 5 DEEKTAVIDHPIFDRKDDGKHTLIIADEANVAKIPIIVGKVD-----SPVLFKGVGIAAD 169
DEE AVIDH +D D G+HT I+ N+ + IVGK + +P+L++G G+ AD
Sbjct: 129 DEENAAVIDHLHYDVSDAGEHTTILTSAKNLIQADTIVGKANRQADAAPLLYRGTGLIAD 188
Query: 170 DQNPLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFS 349
+NPL++ LL A S+AYS+NP+ V DYP++VG T LI ALQARNNARV+F GSL FS
Sbjct: 189 KENPLVLKLLTAESTAYSYNPEASVSDYPHAVGRGTLLIAALQARNNARVVFSGSLLFFS 248
Query: 350 DYLF 361
D F
Sbjct: 249 DESF 252
>sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor
(Oligosaccharyl transferase 48 kDa subunit)
Length = 445
Score = 91.7 bits (226), Expect = 5e-19
Identities = 49/123 (39%), Positives = 74/123 (60%), Gaps = 3/123 (2%)
Frame = +2
Query: 2 FDEEKTAVIDHPIFDRK-DDGKHTLIIADEANVAKIPIIVGKVDS--PVLFKGVGIAADD 172
F+E T+VIDH +D+ D G HT ++ + + +IVG PVLFKG+G+ A
Sbjct: 125 FEEAGTSVIDHHNYDQTLDSGDHTTLVVGKDQLISAELIVGNSAKLHPVLFKGIGLVAGK 184
Query: 173 QNPLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSD 352
N L ++++ AS +AYS++P P+ G T L+ LQ+RNNAR++F GS +LFS+
Sbjct: 185 TNNLALSIVRASGTAYSYDPKAVRATNPSIAGSRTLLVGGLQSRNNARIVFTGSSELFSN 244
Query: 353 YLF 361
F
Sbjct: 245 TFF 247
>sp|P09147|GALE_ECOLI UDP-glucose 4-epimerase (Galactowaldenase) (UDP-galactose
4-epimerase)
Length = 338
Score = 29.3 bits (64), Expect = 3.0
Identities = 15/50 (30%), Positives = 26/50 (52%)
Frame = +2
Query: 203 ASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSD 352
A A + KP++ Y N+V +LI+A++A N +F S ++ D
Sbjct: 81 AGLKAVGESVQKPLEYYDNNVNGTLRLISAMRAANVKNFIFSSSATVYGD 130
>sp|Q56093|GALE_SALTI UDP-glucose 4-epimerase (Galactowaldenase) (UDP-galactose
4-epimerase)
Length = 338
Score = 29.3 bits (64), Expect = 3.0
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = +2
Query: 236 KPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSD 352
KP++ Y N+V +L++A++A N ++F S ++ D
Sbjct: 92 KPLEYYDNNVNGTLRLVSAMRAANVKNLIFSSSATVYGD 130
>sp|Q8XJP7|TRMD_CLOPE tRNA (Guanine-N(1)-)-methyltransferase (M1G-methyltransferase)
(tRNA [GM37] methyltransferase)
Length = 236
Score = 28.9 bits (63), Expect = 3.9
Identities = 15/46 (32%), Positives = 22/46 (47%), Gaps = 8/46 (17%)
Frame = +2
Query: 218 YSFNPDKPVDDYPNSVGIN--------TQLITALQARNNARVLFVG 331
Y+ N K VDDYP G I A++ +N +V+F+G
Sbjct: 40 YTLNKHKKVDDYPYGGGAGMVMTPQPVVDAIKAVKEKNKGKVIFLG 85
>sp|Q9FI00|ML11_ARATH MLO-like protein 11 (AtMlo11)
Length = 573
Score = 28.5 bits (62), Expect = 5.1
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -3
Query: 111 IGILATLASSAIINVCFPSSF 49
I +L T SS I N+C PSSF
Sbjct: 76 ISLLLTATSSTIANICVPSSF 96
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,546,639
Number of Sequences: 369166
Number of extensions: 646412
Number of successful extensions: 1701
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1664
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1692
length of database: 68,354,980
effective HSP length: 87
effective length of database: 52,283,035
effective search space used: 1725340155
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)