Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_J20
(917 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--pr... 335 1e-91
sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--pr... 332 9e-91
sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--pr... 331 2e-90
sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--pr... 327 2e-89
sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--pr... 321 2e-87
sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosacch... 248 2e-65
sp|Q29381|OST48_PIG Dolichyl-diphosphooligosaccharide--prot... 123 8e-28
sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--pro... 66 2e-10
sp|Q01811|VNSS_INSVN Nonstructural protein NS-S 35 0.23
sp|P53882|YNR6_YEAST Hypothetical 67.4 kDa protein in RPS3-... 35 0.29
>sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 439
Score = 335 bits (859), Expect = 1e-91
Identities = 168/290 (57%), Positives = 219/290 (75%), Gaps = 2/290 (0%)
Frame = +2
Query: 53 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 232
TLVLL+ +ETHS+FF+SL++RG +LT+ ADD SL L K+GE+LY N+IIF+PSV +
Sbjct: 30 TLVLLDNLNVRETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVED 89
Query: 233 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFDEEKTAVIDHHNFDRKD 412
FGG I+V T+ F+D GG++LVAASS IG+ +RELGSECGIEFDEEKTAVIDHHN+D D
Sbjct: 90 FGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDISD 149
Query: 413 DGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNP 589
G+HTLI+AD N+ K P IVGK +P+LF+GVG+ AD NPL++++L SS++YSF P
Sbjct: 150 LGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFP 209
Query: 590 DKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARS 766
DKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++
Sbjct: 210 DKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQT 269
Query: 767 GNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEI 916
GN LA A+S+WVFKE GVLRVG VSH ++ P YT+ + V Y I
Sbjct: 270 GNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAP-PNAYTVTDLVEYSI 318
>sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 441
Score = 332 bits (851), Expect = 9e-91
Identities = 167/290 (57%), Positives = 219/290 (75%), Gaps = 2/290 (0%)
Frame = +2
Query: 53 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 232
TLVLL+ ++THS+FF+SL++RG +LT+ ADD SL L K+GE+LY N+IIF+PSV +
Sbjct: 32 TLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVED 91
Query: 233 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFDEEKTAVIDHHNFDRKD 412
FGG I+V T+ F+D GG++LVAASS IG+ +RELGSECGIEFDEEKTAVIDHHN+D D
Sbjct: 92 FGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSD 151
Query: 413 DGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNP 589
G+HTLI+AD N+ K P IVGK +P+LF+GVG+ AD NPL++++L SS++YSF P
Sbjct: 152 LGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFP 211
Query: 590 DKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARS 766
DKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++
Sbjct: 212 DKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQT 271
Query: 767 GNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEI 916
GN LA A+S+WVFKE GVLRVG VSH + + P YT+ + V Y I
Sbjct: 272 GNYELAVALSRWVFKEEGVLRVGPVSHHRVGE-MAPPNAYTVTDLVEYSI 320
>sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit)
Length = 445
Score = 331 bits (848), Expect = 2e-90
Identities = 166/290 (57%), Positives = 218/290 (75%), Gaps = 2/290 (0%)
Frame = +2
Query: 53 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 232
TLVLL+ +ETHS+FF+SL++R +LT+ ADD SL L K+GE+LY N+IIF+PSV +
Sbjct: 36 TLVLLDNLNLRETHSLFFRSLKDRAFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVED 95
Query: 233 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFDEEKTAVIDHHNFDRKD 412
FGG I+V T+ F+D GG++LVAASS IG+ +RELGSECGIEFDEEKTAVIDHHN+D D
Sbjct: 96 FGGNINVETISTFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSD 155
Query: 413 DGKHTLIIADEANVAKIPIIVGKVD-SPVLFKGVGIAADDQNPLLINLLHASSSAYSFNP 589
G+HTLI+AD N+ K P IVG+ +P+LF+GVG+ AD NPL++++L SS++YSF P
Sbjct: 156 LGQHTLIVADPENLLKAPTIVGRSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFP 215
Query: 590 DKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARS 766
DKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V+ A Q ++++
Sbjct: 216 DKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAFFNSAVQKAAPGSQRYSQT 275
Query: 767 GNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEI 916
GN LA A+S+WVFKE GVLRVG VSH ++ P YT+ + V Y I
Sbjct: 276 GNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAP-PNAYTVTDLVEYSI 324
>sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(OST 50 kDa subunit) (DDOST 48 kDa subunit)
Length = 413
Score = 327 bits (839), Expect = 2e-89
Identities = 163/290 (56%), Positives = 219/290 (75%), Gaps = 2/290 (0%)
Frame = +2
Query: 53 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 232
+LVLLE ++THS+FF+SL +RG +LT+ ADD+ L L K+GE+LY N+IIF+PS+ +
Sbjct: 4 SLVLLENLNLRDTHSLFFRSLADRGFELTFRTADDAGLSLIKYGEFLYDNLIIFSPSIED 63
Query: 233 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFDEEKTAVIDHHNFDRKD 412
FGG I+V T+ F+D GG++LVAASS IG+ +RELGSECGIEFDEE+TAVIDHHN+D D
Sbjct: 64 FGGNINVETITAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEERTAVIDHHNYDISD 123
Query: 413 DGKHTLIIADEANVAKIPIIVG-KVDSPVLFKGVGIAADDQNPLLINLLHASSSAYSFNP 589
G+HTLI+AD N+ K P IVG K +P+LF+GVG+ AD NPL++++L SS++YSF P
Sbjct: 124 PGQHTLIVADAENLLKAPTIVGKKALNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFP 183
Query: 590 DKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASVEHAI-SKQTHARS 766
DKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F+++V+ A + ++++
Sbjct: 184 DKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAFFSSAVQKAAPGSKRYSQT 243
Query: 767 GNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHYEI 916
GN LA A+S+WVFKE GVLRVG VSH + + P YT+ + V Y I
Sbjct: 244 GNYELAVALSRWVFKEEGVLRVGAVSHHRVGE-LAPPNAYTVTDLVEYSI 292
>sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit) (OST5OP) (DrOST)
(DmOST50)
Length = 449
Score = 321 bits (823), Expect = 2e-87
Identities = 168/312 (53%), Positives = 217/312 (69%), Gaps = 10/312 (3%)
Frame = +2
Query: 11 LFVSLCVSSCFAI-----DTLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILN 175
L L ++ C A+ +TLVLL+ A +ETHSIFFKSL++RG KLTY LADDSSL+L+
Sbjct: 7 LIAVLAIAHCQAVLETDANTLVLLDNLAIRETHSIFFKSLQDRGFKLTYKLADDSSLLLS 66
Query: 176 KFGEYLYKNVIIFAPSVREFGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGI 355
K+GEYLYKNVIIFAPSV EFGG + V L FVD+GGN+LVA S K G+A+RE SECG
Sbjct: 67 KYGEYLYKNVIIFAPSVEEFGGDVSVERLAQFVDDGGNVLVAGSEKSGDALREFASECGF 126
Query: 356 EFDEEKTAVIDHHNFDRKDDGKHTLIIADEANVAKIPIIVGKVD-----SPVLFKGVGIA 520
E DEE AVIDH ++D D G+HT I+ N+ + IVGK + +P+L++G G+
Sbjct: 127 ELDEENAAVIDHLHYDVSDAGEHTTILTSAKNLIQADTIVGKANRQADAAPLLYRGTGLI 186
Query: 521 ADDQNPLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDL 700
AD +NPL++ LL A S+AYS+NP+ V DYP++VG T LI ALQARNNARV+F GSL
Sbjct: 187 ADKENPLVLKLLTAESTAYSYNPEASVSDYPHAVGRGTLLIAALQARNNARVVFSGSLLF 246
Query: 701 FSDDLFTASVEHAISKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENPR 880
FSD+ FT +V++A S H +GN +A +ISKWVF ETG LRV +V H + + +
Sbjct: 247 FSDESFTTAVQYAQSGVFHKLAGNRDVAESISKWVFGETGRLRVASVQHHKEGELLPPDQ 306
Query: 881 EYTIMENVHYEI 916
YTI + V Y I
Sbjct: 307 AYTITDPVVYTI 318
>sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor
(Oligosaccharyl transferase 48 kDa subunit)
Length = 445
Score = 248 bits (633), Expect = 2e-65
Identities = 134/307 (43%), Positives = 187/307 (60%), Gaps = 3/307 (0%)
Frame = +2
Query: 5 LFLFVSLCVSSCFAIDTLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFG 184
L L S+ A LVL ET A K+THS+F S++ERGH+LT ADDS L L K G
Sbjct: 7 LLLIASIGFHQSLADRVLVLGETAAVKDTHSVFLNSVKERGHELTVRAADDSQLALFKHG 66
Query: 185 EYLYKNVIIFAPSVREFGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECGIEFD 364
+ ++ ++ I AP V+ FGG + + FVD GGN+LVAA S IG+A+RE+ +E G EF+
Sbjct: 67 QLIFDHLFILAPGVQVFGGSLSPSEISKFVDAGGNVLVAAGSNIGDALREIAAEHGFEFE 126
Query: 365 EEKTAVIDHHNFDRK-DDGKHTLIIADEANVAKIPIIVGKVDS--PVLFKGVGIAADDQN 535
E T+VIDHHN+D+ D G HT ++ + + +IVG PVLFKG+G+ A N
Sbjct: 127 EAGTSVIDHHNYDQTLDSGDHTTLVVGKDQLISAELIVGNSAKLHPVLFKGIGLVAGKTN 186
Query: 536 PLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDL 715
L ++++ AS +AYS++P P+ G T L+ LQ+RNNAR++F GS +LFS+
Sbjct: 187 NLALSIVRASGTAYSYDPKAVRATNPSIAGSRTLLVGGLQSRNNARIVFTGSSELFSNTF 246
Query: 716 FTASVEHAISKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIM 895
F+A A+SGN ATAI++WV KE+GVLRV V+H ++V Y I
Sbjct: 247 FSAKTNSVNPSVQGAQSGNADFATAITRWVMKESGVLRVKTVNHHKKGETVPPVEGYFIT 306
Query: 896 ENVHYEI 916
E+V Y I
Sbjct: 307 EDVVYTI 313
>sp|Q29381|OST48_PIG Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(DDOST 48 kDa subunit)
Length = 103
Score = 123 bits (308), Expect = 8e-28
Identities = 58/100 (58%), Positives = 77/100 (77%)
Frame = +2
Query: 53 TLVLLETFATKETHSIFFKSLRERGHKLTYHLADDSSLILNKFGEYLYKNVIIFAPSVRE 232
T VLL+ +ETHS+FF+SL++RG LT+ ADD SL L K+GE+LY N+I+F+PSV +
Sbjct: 4 TXVLLDNLNLRETHSLFFRSLKDRGFVLTFKTADDPSLSLIKYGEFLYDNLIVFSPSVED 63
Query: 233 FGGKIDVPTLMNFVDEGGNILVAASSKIGEAIRELGSECG 352
FGG I+V T+ F+D GG+ LVAASS IG+ +R LGSECG
Sbjct: 64 FGGNINVETISTFIDGGGSXLVAASSDIGDPLRXLGSECG 103
>sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase beta
subunit precursor (Oligosaccharyl transferase beta
subunit)
Length = 430
Score = 65.9 bits (159), Expect = 2e-10
Identities = 66/310 (21%), Positives = 129/310 (41%), Gaps = 5/310 (1%)
Frame = +2
Query: 2 FLFLFVSLCVSSCFAIDTLVLLETFATK-ETHSIFFKSLRERGHKLTYHLADDSSLILNK 178
F L ++ V TLVL + E +S++ K L +R +KL Y + +S ++
Sbjct: 9 FCILLQAIFVVGTQTSRTLVLYDQSTEPLEEYSVYLKDLEQRNYKLEYLDINSTSTTVDL 68
Query: 179 FG--EYLYKNVIIF-APSVREFGGKIDVPTLMNFVDEGGNILVAAS-SKIGEAIRELGSE 346
+ + L+ N+I+F + +I V L+ F + GNIL +S + IR +E
Sbjct: 69 YDKEQRLFDNIIVFPTKGGKNLARQIPVKQLIKFFENEGNILCMSSPGAVPNTIRLFLNE 128
Query: 347 CGIEFDEEKTAVIDHHNFDRKDDGKHTLIIADEANVAKIPIIVGKVDSPVLFKGVGIAAD 526
GI + + D+ + ++ ++ ++ + + +F A
Sbjct: 129 LGIYPSPKGHVIRDYFSPSSEE------LVVSSNHLLNKYVYNARKSEDFVFGESSAALL 182
Query: 527 DQNPLLINLLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFS 706
+ ++ +L+A ++++ + K S G L+ Q NNAR++++GS D
Sbjct: 183 ENREQIVPILNAPRTSFTESKGKCNSWTSGSQGF---LVVGFQNLNNARLVWIGSSDFL- 238
Query: 707 DDLFTASVEHAISKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreY 886
+ + N+ A + KW F E V++ + HS AD + + Y
Sbjct: 239 --------------KNKNQDSNQEFAKELLKWTFNEKSVIKSVHAVHSHADGTSYDEEPY 284
Query: 887 TIMENVHYEI 916
I + V Y +
Sbjct: 285 KIKDKVIYSV 294
>sp|Q01811|VNSS_INSVN Nonstructural protein NS-S
Length = 449
Score = 35.4 bits (80), Expect = 0.23
Identities = 34/138 (24%), Positives = 58/138 (42%), Gaps = 15/138 (10%)
Frame = -3
Query: 369 SSSNSIPHSLPNSLIASPILELAATKILPPSST-KFIKVGTSIFPPNSLTDGANM----- 208
+++N + H +++S +L + +I P T +F + IFPPN+ + AN
Sbjct: 104 TNTNGVKHQGHLKVLSSQLLRMLEEQIAVPEITSRFGLKESDIFPPNNFIEAANKGSLSC 163
Query: 207 ---MTFLYKYSPN--LFKIS----EESSAKW*VNLCPRSRSDLKNIECVSLVANVSNSTK 55
+ F KYS N + K+S S +W L P N V+ +A S +
Sbjct: 164 VKEVLFDVKYSNNQSMGKVSVLSPTRSVHEWLYTLKPVFNQSQTNNRTVNTLAVKSLAMS 223
Query: 54 VSIAKHEETHKLTKRNKN 1
+ +TH + N N
Sbjct: 224 ATSDLMSDTHSFVRLNNN 241
>sp|P53882|YNR6_YEAST Hypothetical 67.4 kDa protein in RPS3-PSD1 intergenic region
Length = 636
Score = 35.0 bits (79), Expect = 0.29
Identities = 32/109 (29%), Positives = 50/109 (45%), Gaps = 2/109 (1%)
Frame = -3
Query: 381 TAVFSSSNSIPHSLPNSLIASPILELAATKILPPSSTKFIKVGTSIFPPNSLTDGANMMT 202
T++ SS +S+ S P+SL+ S L L+ + + +SIF P++ T + T
Sbjct: 155 TSLSSSWSSL--SSPSSLLVSSSLSLSLSSSYSDTKLFSFDSRSSIFSPSTPTVISPSYT 212
Query: 201 FLYKYSPNLFKIS--EESSAKW*VNLCPRSRSDLKNIECVSLVANVSNS 61
+L S F+IS E S+ W + S K+ C S N S S
Sbjct: 213 YLSSISATSFQISTTSELSSSWFSTISSPSTISNKDTTCPSSSRNTSTS 261
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,470,604
Number of Sequences: 369166
Number of extensions: 2038460
Number of successful extensions: 6075
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5799
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6039
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9366655350
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)