Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_I24
(566 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 103 3e-22
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 102 5e-22
sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20 99 1e-20
sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 ... 97 3e-20
sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p... 96 8e-20
sp|Q10056|SHK2_SCHPO Serine/threonine-protein kinase shk2 95 1e-19
sp|Q60700|M3K12_MOUSE Mitogen-activated protein kinase kina... 95 1e-19
sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 ... 95 1e-19
sp|Q12852|M3K12_HUMAN Mitogen-activated protein kinase kina... 95 1e-19
sp|Q63796|M3K12_RAT Mitogen-activated protein kinase kinase... 94 2e-19
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 103 bits (257), Expect = 3e-22
Identities = 73/186 (39%), Positives = 99/186 (53%), Gaps = 1/186 (0%)
Frame = +2
Query: 5 GTRFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTAN 184
G +LH+ I+HRD+KG IL+DN IKL F ++K + ++ T A+S GT
Sbjct: 184 GLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTG----AKSMKGTPY 239
Query: 185 WTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPL 361
W APEVI G+ SADIWS G ++E++TGKAP AI+ +GT K
Sbjct: 240 WMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDT 299
Query: 362 KCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSAMEAD*FT*RVDIFSWIN*NNTYML 541
SDA K F+ CL P RPT +L+KH F+ + T D+ S +N N + L
Sbjct: 300 LSSDA-KDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESAST---DLGSVLN-NLSTPL 354
Query: 542 PK*INN 559
P INN
Sbjct: 355 PLQINN 360
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 102 bits (255), Expect = 5e-22
Identities = 61/153 (39%), Positives = 84/153 (54%), Gaps = 1/153 (0%)
Frame = +2
Query: 5 GTRFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTAN 184
G +LH+ I+HRD+KG IL+DN IKL F ++K + ++ + A+S GT
Sbjct: 183 GLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISG----AKSMKGTPY 238
Query: 185 WTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPL 361
W APEVI G+ SADIWS G ++E++TGKAP AI+ +GT K P
Sbjct: 239 WMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKS-HPPIPD 297
Query: 362 KCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 460
S F+ CL EP RPT +L+KH F+
Sbjct: 298 NISSDANDFLLKCLQQEPNLRPTASELLKHPFV 330
>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
Length = 939
Score = 98.6 bits (244), Expect = 1e-20
Identities = 55/159 (34%), Positives = 81/159 (50%)
Frame = +2
Query: 5 GTRFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTAN 184
G FLHS+ +LHRD+K ILL DIKL F + N + GT
Sbjct: 726 GLEFLHSKGVLHRDIKSDNILLSMEGDIKLTDFGFCAQINEL------NLKRTTMVGTPY 779
Query: 185 WTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLK 364
W APEV++ + YG DIWS G +++E+I G+ P +A+Y + T + P
Sbjct: 780 WMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPEN 839
Query: 365 CSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSAMEAD 481
S +LK F+D CL EP +R + +L+ E++ EA+
Sbjct: 840 LSSSLKKFLDWCLCVEPEDRASATELLHDEYITEIAEAN 878
>sp|Q13153|PAK1_HUMAN Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK)
Length = 545
Score = 97.1 bits (240), Expect = 3e-20
Identities = 53/153 (34%), Positives = 81/153 (52%), Gaps = 1/153 (0%)
Frame = +2
Query: 14 FLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWT 190
FLHS ++HRD+K ILL + +KL F A++ P+ S + + GT W
Sbjct: 379 FLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------TMVGTPYWM 431
Query: 191 APEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCS 370
APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E P K S
Sbjct: 432 APEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLS 491
Query: 371 DALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 469
+ F++ CLD + R + K+L++H+FL A
Sbjct: 492 AIFRDFLNRCLDMDVEKRGSAKELLQHQFLKIA 524
>sp|P35465|PAK1_RAT Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P68-PAK) (Alpha-PAK) (Protein kinase MUK2)
Length = 544
Score = 95.5 bits (236), Expect = 8e-20
Identities = 52/153 (33%), Positives = 81/153 (52%), Gaps = 1/153 (0%)
Frame = +2
Query: 14 FLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWT 190
FLHS ++HRD+K ILL + +KL F A++ P+ S + + GT W
Sbjct: 378 FLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------TMVGTPYWM 430
Query: 191 APEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCS 370
APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E P K S
Sbjct: 431 APEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLS 490
Query: 371 DALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 469
+ F++ CL+ + R + K+L++H+FL A
Sbjct: 491 AIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIA 523
>sp|Q10056|SHK2_SCHPO Serine/threonine-protein kinase shk2
Length = 589
Score = 95.1 bits (235), Expect = 1e-19
Identities = 52/155 (33%), Positives = 80/155 (51%)
Frame = +2
Query: 5 GTRFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTAN 184
G + LH++NI+HRD+K +LLDN+ +IK+ F + R + + GT
Sbjct: 421 GIQHLHARNIIHRDIKSDNVLLDNSGNIKITDFGFCARLSN------RTNKRVTMVGTPY 474
Query: 185 WTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLK 364
W APEV+ YG+ DIWS G +++E+I + P +A+Y + P
Sbjct: 475 WMAPEVVKQNEYGTKVDIWSLGIMIIEMIENEPPYLREDPIRALYLIAKNGTPTLKKPNL 534
Query: 365 CSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 469
S LKSF++ CL + + R T +L+ H FLN A
Sbjct: 535 VSKNLKSFLNSCLTIDTIFRATAAELLTHSFLNQA 569
>sp|Q60700|M3K12_MOUSE Mitogen-activated protein kinase kinase kinase 12 (Mixed lineage
kinase) (Leucine-zipper protein kinase) (ZPK) (Dual
leucine zipper bearing kinase) (DLK) (MAPK-upstream
kinase) (MUK)
Length = 888
Score = 94.7 bits (234), Expect = 1e-19
Identities = 53/155 (34%), Positives = 80/155 (51%)
Frame = +2
Query: 5 GTRFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTAN 184
G +LH I+HRDLK P +L+ + +K+ F ++K D S++ S GT
Sbjct: 256 GMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKM-------SFAGTVA 308
Query: 185 WTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLK 364
W APEVI E DIWSFG V+ EL+TG+ P + S I+ +G+ + P
Sbjct: 309 WMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGS-NSLHLPVPSS 367
Query: 365 CSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 469
C D K + C + +P NRP+ + ++ H + SA
Sbjct: 368 CPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASA 402
>sp|O88643|PAK1_MOUSE Serine/threonine-protein kinase PAK 1 (p21-activated kinase 1)
(PAK-1) (P65-PAK) (Alpha-PAK) (CDC42/RAC effector kinase
PAK-A)
Length = 545
Score = 94.7 bits (234), Expect = 1e-19
Identities = 52/153 (33%), Positives = 80/153 (52%), Gaps = 1/153 (0%)
Frame = +2
Query: 14 FLHSQNILHRDLKGPKILLDNNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWT 190
FLHS ++HRD+K ILL + +KL F A++ P+ S + + GT W
Sbjct: 379 FLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRS-------TMVGTPYWM 431
Query: 191 APEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCS 370
APEV+ + YG DIWS G + +E+I G+ P +A+Y + T E P K S
Sbjct: 432 APEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLS 491
Query: 371 DALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 469
+ F+ CL+ + R + K+L++H+FL A
Sbjct: 492 AIFRDFLQCCLEMDVEKRGSAKELLQHQFLKIA 524
>sp|Q12852|M3K12_HUMAN Mitogen-activated protein kinase kinase kinase 12 (Mixed lineage
kinase) (Leucine-zipper protein kinase) (ZPK) (Dual
leucine zipper bearing kinase) (DLK) (MAPK-upstream
kinase) (MUK)
Length = 859
Score = 94.7 bits (234), Expect = 1e-19
Identities = 53/155 (34%), Positives = 80/155 (51%)
Frame = +2
Query: 5 GTRFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTAN 184
G +LH I+HRDLK P +L+ + +K+ F ++K D S++ S GT
Sbjct: 223 GMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKM-------SFAGTVA 275
Query: 185 WTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLK 364
W APEVI E DIWSFG V+ EL+TG+ P + S I+ +G+ + P
Sbjct: 276 WMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGS-NSLHLPVPSS 334
Query: 365 CSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 469
C D K + C + +P NRP+ + ++ H + SA
Sbjct: 335 CPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASA 369
>sp|Q63796|M3K12_RAT Mitogen-activated protein kinase kinase kinase 12 (Mixed lineage
kinase) (Leucine-zipper protein kinase) (ZPK) (Dual
leucine zipper bearing kinase) (DLK) (MAPK-upstream
kinase) (MUK)
Length = 888
Score = 94.4 bits (233), Expect = 2e-19
Identities = 53/155 (34%), Positives = 80/155 (51%)
Frame = +2
Query: 5 GTRFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTAN 184
G +LH I+HRDLK P +L+ + +K+ F ++K D S++ S GT
Sbjct: 256 GMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKM-------SFAGTVA 308
Query: 185 WTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLK 364
W APEVI E DIWSFG V+ EL+TG+ P + S I+ +G+ + P
Sbjct: 309 WMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGS-NSLHLPVPSS 367
Query: 365 CSDALKSFVDPCLDPEPLNRPTVKDLMKHEFLNSA 469
C D K + C + +P NRP+ + ++ H + SA
Sbjct: 368 CPDGFKILLRQCWNRKPRNRPSFRQILLHLDIASA 402
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,322,382
Number of Sequences: 369166
Number of extensions: 1383887
Number of successful extensions: 6598
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4974
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5214
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4127973360
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)