Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_E13
(703 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q24799|MYPH_ECHGR Myophilin 224 1e-58
sp|P14318|MP20_DROME Muscle-specific protein 20 167 2e-41
sp|Q08092|CNN1_PIG Calponin-1 (Calponin H1, smooth muscle) ... 124 2e-28
sp|Q08290|CNN1_RAT Calponin-1 (Calponin H1, smooth muscle) ... 124 2e-28
sp|Q08091|CNN1_MOUSE Calponin-1 (Calponin H1, smooth muscle... 123 5e-28
sp|P51911|CNN1_HUMAN Calponin-1 (Calponin H1, smooth muscle... 122 9e-28
sp|P26932|CNN1_CHICK Calponin-1 (Calponin, smooth muscle) 120 3e-27
sp|P37803|CNN1_MELGA Calponin-1 (Calponin, smooth muscle) 117 3e-26
sp|Q9UI15|TAGL3_HUMAN Transgelin-3 (Neuronal protein NP25) ... 114 2e-25
sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-a... 114 3e-25
>sp|Q24799|MYPH_ECHGR Myophilin
Length = 190
Score = 224 bits (572), Expect = 1e-58
Identities = 109/157 (69%), Positives = 127/157 (80%)
Frame = +2
Query: 35 GLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSKSYEDNLKSGVILCKVMNKLQPG 214
GLS QVKKKL KRD QE E +EWI+++ KLD SK YED LK G +LCK+MN ++PG
Sbjct: 9 GLSYQVKKKLEGKRDKDQENEALEWIEALTGLKLDRSKLYEDILKDGTVLCKLMNSIKPG 68
Query: 215 IIKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEKKDIAQVTQSLFALGR 394
IKKINEN TMPFKIMENI+AF EA+K YGVPV D+FQTVDL+EKKDIAQVT++LFALGR
Sbjct: 69 CIKKINENATMPFKIMENISAFLEAMKGYGVPVADLFQTVDLFEKKDIAQVTRTLFALGR 128
Query: 395 TCQTHADYHGPVLGPKLAQENKRGFAEDDIGEGSSIV 505
TCQTH +Y GPVLGPKLA ENKR F E + EG ++V
Sbjct: 129 TCQTHPEYSGPVLGPKLATENKREFTEQQLREGQNVV 165
>sp|P14318|MP20_DROME Muscle-specific protein 20
Length = 184
Score = 167 bits (424), Expect = 2e-41
Identities = 83/156 (53%), Positives = 108/156 (69%)
Frame = +2
Query: 38 LSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSKSYEDNLKSGVILCKVMNKLQPGI 217
L V+ K+ KR+P ++E EWI++I+ EK + +SYED LK G +LCK++N L P
Sbjct: 3 LERAVRAKIASKRNPEMDKEAQEWIEAIIAEKFPAGQSYEDVLKDGQVLCKLINVLSPNA 62
Query: 218 IKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEKKDIAQVTQSLFALGRT 397
+ K+N + FK MENIN FQ+A+K+YGVP DVFQTVDLYEKKDIA VT ++FALGR
Sbjct: 63 VPKVNSSGGQ-FKFMENINNFQKALKEYGVPDIDVFQTVDLYEKKDIANVTNTIFALGRA 121
Query: 398 CQTHADYHGPVLGPKLAQENKRGFAEDDIGEGSSIV 505
HAD+ GP LGPK A E KR F E+ + G +IV
Sbjct: 122 TYKHADFKGPFLGPKPADECKRDFTEEQLKAGQTIV 157
>sp|Q08092|CNN1_PIG Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 124 bits (312), Expect = 2e-28
Identities = 68/163 (41%), Positives = 102/163 (62%)
Frame = +2
Query: 17 NQGVAYGLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSKSYEDNLKSGVILCKVM 196
N+G AYGLSA+VK KL +K D QEQE+ EWI+ + ++ ++ D LK G+ILC+ +
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDHQQEQELREWIEGVTGRRI--GNNFMDGLKDGIILCEFI 64
Query: 197 NKLQPGIIKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEKKDIAQVTQS 376
NKLQPG +KK+NE+ T + +ENI F +AI KYGV D+F+ DL+E + QV +
Sbjct: 65 NKLQPGSVKKVNES-TQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQST 123
Query: 377 LFALGRTCQTHADYHGPVLGPKLAQENKRGFAEDDIGEGSSIV 505
L AL +T + +G K A++ +R F + + EG +I+
Sbjct: 124 LLALASMAKTKGNKVN--VGVKYAEKQERKFEPEKLREGRNII 164
>sp|Q08290|CNN1_RAT Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 124 bits (311), Expect = 2e-28
Identities = 68/163 (41%), Positives = 103/163 (63%)
Frame = +2
Query: 17 NQGVAYGLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSKSYEDNLKSGVILCKVM 196
N+G AYGLSA+VK KL +K D +EQE+ EWI+ + ++ S ++ D LK G+ILC+ +
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGS--NFMDGLKDGIILCEFI 64
Query: 197 NKLQPGIIKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEKKDIAQVTQS 376
NKLQPG +KK+NE+ T + +ENI F +AI KYGV D+F+ DL+E + QV +
Sbjct: 65 NKLQPGSVKKVNES-TQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQST 123
Query: 377 LFALGRTCQTHADYHGPVLGPKLAQENKRGFAEDDIGEGSSIV 505
L AL +T + +G K A++ +R F + + EG +I+
Sbjct: 124 LLALASMAKTKGNKVN--VGVKYAEKQERRFEPEKLREGRNII 164
>sp|Q08091|CNN1_MOUSE Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 123 bits (308), Expect = 5e-28
Identities = 67/163 (41%), Positives = 102/163 (62%)
Frame = +2
Query: 17 NQGVAYGLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSKSYEDNLKSGVILCKVM 196
N+G AYGLSA+VK KL +K D +EQE+ EWI+ + ++ ++ D LK G+ILC+ +
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRI--GNNFMDGLKDGIILCEFI 64
Query: 197 NKLQPGIIKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEKKDIAQVTQS 376
NKLQPG +KK+NE+ T + +ENI F +AI KYGV D+F+ DL+E + QV +
Sbjct: 65 NKLQPGSVKKVNES-TQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQST 123
Query: 377 LFALGRTCQTHADYHGPVLGPKLAQENKRGFAEDDIGEGSSIV 505
L AL +T + +G K A++ +R F + + EG +I+
Sbjct: 124 LLALASMAKTKGNKVN--VGVKYAEKQERRFEPEKLREGRNII 164
>sp|P51911|CNN1_HUMAN Calponin-1 (Calponin H1, smooth muscle) (Basic calponin)
Length = 297
Score = 122 bits (306), Expect = 9e-28
Identities = 68/163 (41%), Positives = 101/163 (61%)
Frame = +2
Query: 17 NQGVAYGLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSKSYEDNLKSGVILCKVM 196
N+G AYGLSA+VK KL +K D +EQE+ EWI+ + ++ ++ D LK G+ILC+ +
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRI--GNNFMDGLKDGIILCEFI 64
Query: 197 NKLQPGIIKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEKKDIAQVTQS 376
NKLQPG +KKINE+ T + +ENI F +AI KYGV D+F+ DL+E + QV +
Sbjct: 65 NKLQPGSVKKINES-TQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQST 123
Query: 377 LFALGRTCQTHADYHGPVLGPKLAQENKRGFAEDDIGEGSSIV 505
L AL +T + +G K A++ +R F + EG +I+
Sbjct: 124 LLALASMAKTKGNKVN--VGVKYAEKQERKFEPGKLREGRNII 164
>sp|P26932|CNN1_CHICK Calponin-1 (Calponin, smooth muscle)
Length = 292
Score = 120 bits (302), Expect = 3e-27
Identities = 66/163 (40%), Positives = 100/163 (61%)
Frame = +2
Query: 17 NQGVAYGLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSKSYEDNLKSGVILCKVM 196
N+G AYGLSA+VK KL +K DP E+++ WI+ ++ + + D LK GVILC+++
Sbjct: 7 NRGPAYGLSAEVKNKLAQKYDPQTERQLRVWIEGATGRRIGDN--FMDGLKDGVILCELI 64
Query: 197 NKLQPGIIKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEKKDIAQVTQS 376
NKLQPG ++K+N+ P + +ENI F AIK YGV D+F+ DL+E + QV +
Sbjct: 65 NKLQPGSVQKVND-PVQNWHKLENIGNFLRAIKHYGVKPHDIFEANDLFENTNHTQVQST 123
Query: 377 LFALGRTCQTHADYHGPVLGPKLAQENKRGFAEDDIGEGSSIV 505
L AL +T + G LG K A++ +R F + + EG +I+
Sbjct: 124 LIALASQAKTKGNNVG--LGVKYAEKQQRRFQPEKLREGRNII 164
>sp|P37803|CNN1_MELGA Calponin-1 (Calponin, smooth muscle)
Length = 176
Score = 117 bits (293), Expect = 3e-26
Identities = 65/163 (39%), Positives = 99/163 (60%)
Frame = +2
Query: 17 NQGVAYGLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSKSYEDNLKSGVILCKVM 196
N+G AYGLSA+VK KL +K DP E+++ WI+ ++ + + D LK GVIL +++
Sbjct: 1 NRGPAYGLSAEVKNKLAQKYDPQTERQLRVWIEGATGRRIGDN--FXDGLKDGVILMELI 58
Query: 197 NKLQPGIIKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEKKDIAQVTQS 376
NKLQPG ++K+N+ P + +ENI F AIK YGV D+F+ DL+E + QV +
Sbjct: 59 NKLQPGSVQKVND-PVQNWHKLENIGNFLRAIKHYGVKPHDIFEANDLFENTNHTQVQST 117
Query: 377 LFALGRTCQTHADYHGPVLGPKLAQENKRGFAEDDIGEGSSIV 505
L AL +T + G LG K A++ +R F + + EG +I+
Sbjct: 118 LIALASQAKTKGNNVG--LGVKYAEKQQRRFQPEKLREGRNII 158
>sp|Q9UI15|TAGL3_HUMAN Transgelin-3 (Neuronal protein NP25) (Neuronal protein 22) (NP22)
Length = 199
Score = 114 bits (285), Expect = 2e-25
Identities = 68/175 (38%), Positives = 107/175 (61%), Gaps = 10/175 (5%)
Frame = +2
Query: 11 VANQGVAYGLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLD----SSKSYEDNLKSGV 178
+AN+G +YGLS +V++K+ +K D E ++++WI E ++ ++ L G
Sbjct: 1 MANRGPSYGLSREVQEKIEQKYDADLENKLVDWIILQCAEDIEHPPPGRAHFQKWLMDGT 60
Query: 179 ILCKVMNKLQPG---IIKKINENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEK 349
+LCK++N L P I KI+E+ M FK ME I+ F +A + YGV TD+FQTVDL+E
Sbjct: 61 VLCKLINSLYPPGQEPIPKISESK-MAFKQMEQISQFLKAAETYGVRTTDIFQTVDLWEG 119
Query: 350 KDIAQVTQSLFALGRTCQTHAD--YHG-PVLGPKLAQENKRGFAEDDIGEGSSIV 505
KD+A V ++L ALG T D Y G P + AQ+N+RGF+E+ + +G +++
Sbjct: 120 KDMAAVQRTLMALGSVAVTKDDGCYRGEPSWFHRKAQQNRRGFSEEQLRQGQNVI 174
>sp|P19966|TAGL_CHICK Transgelin (Smooth muscle protein 22-alpha) (SM22-alpha)
Length = 200
Score = 114 bits (284), Expect = 3e-25
Identities = 70/175 (40%), Positives = 102/175 (58%), Gaps = 10/175 (5%)
Frame = +2
Query: 11 VANQGVAYGLSAQVKKKLNEKRDPAQEQEVIEWIQSILNEKLDSSK----SYEDNLKSGV 178
+AN+G AYG+S V+ K+ +K D E ++EWI + + ++ LK+G+
Sbjct: 1 MANKGPAYGMSRDVQSKIEKKYDDELEDRLVEWIVAQCGSSVGRPDRGRLGFQVWLKNGI 60
Query: 179 ILCKVMNKLQPGIIKKI---NENPTMPFKIMENINAFQEAIKKYGVPVTDVFQTVDLYEK 349
+L +++N L P K + + PTM FK ME I F +A + YGV TD+FQTVDL+E
Sbjct: 61 VLSQLVNSLYPDGSKPVKIPDSPPTMVFKQMEQIAQFLKAAEDYGVVKTDMFQTVDLFEA 120
Query: 350 KDIAQVTQSLFALGRTCQTHAD--YHG-PVLGPKLAQENKRGFAEDDIGEGSSIV 505
KD+A V ++L ALG T D YHG P K AQE+KR F+E + EG +I+
Sbjct: 121 KDMAAVQRTLVALGSLAVTKNDGHYHGDPNWFMKKAQEHKREFSESQLKEGKNII 175
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,440,075
Number of Sequences: 369166
Number of extensions: 1654989
Number of successful extensions: 5069
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4764
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4993
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6122130210
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)