Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_D15
(969 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P25037|UBP1_YEAST Ubiquitin carboxyl-terminal hydrolase ... 37 0.064
sp|P62134|RAD50_METMP DNA double-strand break repair rad50 ... 33 0.92
sp|Q9UPN3|MACF1_HUMAN Microtubule-actin crosslinking factor... 33 1.2
sp|Q96PK2|MACF4_HUMAN Microtubule-actin crosslinking factor... 33 1.2
sp|Q03290|TFB3_YEAST RNA polymerase II transcription factor... 33 1.6
sp|P38426|TPS3_YEAST Alpha,alpha-trehalose-phosphate syntha... 33 1.6
sp|Q9QXL2|KI21A_MOUSE Kinesin family member 21A 32 2.1
sp|O33600|RAD50_SULAC DNA double-strand break repair rad50 ... 32 3.5
sp|Q9QXZ0|MACF1_MOUSE Microtubule-actin crosslinking factor... 31 4.6
sp|P05084|HUNB_DROME Hunchback protein 31 4.6
>sp|P25037|UBP1_YEAST Ubiquitin carboxyl-terminal hydrolase 1 (Ubiquitin thiolesterase 1)
(Ubiquitin-specific processing protease 1)
(Deubiquitinating enzyme 1)
Length = 809
Score = 37.4 bits (85), Expect = 0.064
Identities = 41/193 (21%), Positives = 80/193 (41%), Gaps = 13/193 (6%)
Frame = +1
Query: 286 IHQWEHGQEMTKPIVRHEDYSLIQLKNLSSANTEVSEIDQFYSYITRAYKLLLIY----- 450
+HQ E + + KP++ EDY + N+ ++ +I I+R LL I+
Sbjct: 426 VHQIE--EVLAKPVIDDEDYKKLHTANMVRKCSKSKQI-----LISRPPPLLSIHINRSV 478
Query: 451 YEAR--RAKFTNQKQIINDPINIVP-----PQSSAKSRLKNLNKGLSNENDLNKSFDISQ 609
++ R + N K + +N+ P + + +RL K + + D ++ +I
Sbjct: 479 FDPRTYMIRKNNSKVLFKSRLNLAPWCCDINEINLDARLPMSKKEKAAQQDSSEDENIGG 538
Query: 610 ILYFEKHES-DDEFEDAMDEIKFEDMXXXXXXXXXXXXDIDNELKLIANIKAELRNSQHQ 786
Y + HE + EFED+ +E +++D DI N L + + + +
Sbjct: 539 EYYTKLHERFEQEFEDSEEEKEYDDAEGNYASHYNHTKDISNYDPLNGEVDGVTSDDEDE 598
Query: 787 LKLQYDRLIKRVK 825
+ D L +K
Sbjct: 599 YIEETDALGNTIK 611
>sp|P62134|RAD50_METMP DNA double-strand break repair rad50 ATPase
Length = 993
Score = 33.5 bits (75), Expect = 0.92
Identities = 53/272 (19%), Positives = 120/272 (44%), Gaps = 7/272 (2%)
Frame = +1
Query: 55 INLKLDLSKNLRSLYSNFLAEMLNDQSKYEILIFRDC-VGQRMENLSVLYHTENE-NVCH 228
I K++L L+ Y + L+E ++ + EI++ ++ + + + + L T++ +VC
Sbjct: 445 IEEKINLENELKEKYED-LSEKIDKLN--EIVLKKESKISEYKNSKAELEKTKDSCHVCQ 501
Query: 229 YNLMLHSADDIIVKRKLQLIHQWEHGQEMTKP---IVRHEDYSLIQLKNLSSANTEVSEI 399
+ +++ K ++ ++ + + K I+ ++ ++L + S + E+
Sbjct: 502 SKITEEKKQELLEKYNSEIQNEQLSTESLKKQLEIILNKKEKMKVKLNEIDSFKLKYGEL 561
Query: 400 DQFYSYITRAYKLLLIYYEARRAKF--TNQKQIINDPINIVPPQSSAKSRLKNLNKGLSN 573
+ +Y + + ++ E N+ +ND I+++ +++LKNL
Sbjct: 562 KEKKNYSLKVEESIIETTEKLNELTGKINEYSSLNDEISLI------ENKLKNL------ 609
Query: 574 ENDLNKSFDISQILYFEKHESDDEFEDAMDEIKFEDMXXXXXXXXXXXXDIDNELKLIAN 753
END SQ L +DE E +++ + I+NE K + N
Sbjct: 610 ENDYKNCNYSSQFL-----TKNDESEFLTKKLELSKIIGDYDSSK-----IENEKKSLEN 659
Query: 754 IKAELRNSQHQLKLQYDRLIKRVKVSDNPLDS 849
+K EL+N+ + L+ + + L K +K N + S
Sbjct: 660 LKDELKNTIYNLEREIN-LKKELKNIQNDISS 690
>sp|Q9UPN3|MACF1_HUMAN Microtubule-actin crosslinking factor 1, isoforms 1/2/3/5 (Actin
cross-linking family protein 7) (Macrophin-1)
(Trabeculin-alpha) (620 kDa actin-binding protein)
(ABP620)
Length = 5430
Score = 33.1 bits (74), Expect = 1.2
Identities = 23/95 (24%), Positives = 41/95 (43%)
Frame = +1
Query: 622 EKHESDDEFEDAMDEIKFEDMXXXXXXXXXXXXDIDNELKLIANIKAELRNSQHQLKLQY 801
+K++SD +D D+I+ +N+ KL LR HQ K QY
Sbjct: 1586 QKNQSD--LKDLQDDIQNRATSFATVVKDIEGFMEENQTKLSpreLTALREKLHQAKEQY 1643
Query: 802 DRLIKRVKVSDNPLDSIAFASLHPESKAKQMKKPL 906
+ L + +V+ L+ ++L E++ + K L
Sbjct: 1644 EALQEETRVAQKELEEAVTSALQQETEKSKAAKEL 1678
>sp|Q96PK2|MACF4_HUMAN Microtubule-actin crosslinking factor 1, isoform 4
Length = 5938
Score = 33.1 bits (74), Expect = 1.2
Identities = 23/95 (24%), Positives = 41/95 (43%)
Frame = +1
Query: 622 EKHESDDEFEDAMDEIKFEDMXXXXXXXXXXXXDIDNELKLIANIKAELRNSQHQLKLQY 801
+K++SD +D D+I+ +N+ KL LR HQ K QY
Sbjct: 2088 QKNQSD--LKDLQDDIQNRATSFATVVKDIEGFMEENQTKLSpreLTALREKLHQAKEQY 2145
Query: 802 DRLIKRVKVSDNPLDSIAFASLHPESKAKQMKKPL 906
+ L + +V+ L+ ++L E++ + K L
Sbjct: 2146 EALQEETRVAQKELEEAVTSALQQETEKSKAAKEL 2180
>sp|Q03290|TFB3_YEAST RNA polymerase II transcription factor B subunit 3 (RNA polymerase
II transcription factor B p38 subunit) (RNA polymerase
II transcription factor B 38 kDa subunit)
Length = 321
Score = 32.7 bits (73), Expect = 1.6
Identities = 38/155 (24%), Positives = 64/155 (41%), Gaps = 24/155 (15%)
Frame = +1
Query: 211 NENVCHYNLMLHSADDIIVKRKLQLIHQWEHGQEMTKPIVRHEDYSLIQ----LKNLSSA 378
N ++ YN L +DII K +HG ++ K + Y + + NL +
Sbjct: 100 NGDLVEYNKYLEEVEDIIYKL--------DHGIDVAKTEEKLRTYEELNKQLIMNNLERS 151
Query: 379 NTEVSEIDQFYSYIT--RAYKLLL---IYYEARRAKFTNQKQIIN------DPIN----- 510
TE+ +Q + + K LL I E R K +K+I+N IN
Sbjct: 152 RTEIESFEQRQKFEKEMKLKKRLLERQIEEEERMNKEWTKKEIVNRLSTTTQDINETIEG 211
Query: 511 ----IVPPQSSAKSRLKNLNKGLSNENDLNKSFDI 603
+ +SSA+ +L+ LN+ L N N + ++
Sbjct: 212 VKNTVKLKKSSARRKLEELNRVLKNNPYFNSNVNV 246
>sp|P38426|TPS3_YEAST Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 115 kDa
subunit (Trehalose-6-phosphate synthase)
(UDP-glucose-glucosephosphate glucosyltransferase)
Length = 1022
Score = 32.7 bits (73), Expect = 1.6
Identities = 27/103 (26%), Positives = 50/103 (48%), Gaps = 2/103 (1%)
Frame = -3
Query: 619 NIIFEIYQKICLNHFHLIDLYLNFSIET*RMIVEEQY*LDRL*SVFDW*ISLSLLHNKLI 440
NI+ +Y I ++++HL N S+ R +++E++ NK +
Sbjct: 499 NIVSVMYAPIGIDYYHLTSQLRNGSVLEWRQLIKERW------------------RNKKL 540
Query: 439 VICTPE*CRNKIDRFQTLQ--YLHYLNFLIVLKNNLHVEQLVL 317
++ CR++ DR + LQ L Y FLI +N ++E++VL
Sbjct: 541 IV-----CRDQFDRIRGLQKKMLAYERFLI--ENPEYIEKVVL 576
>sp|Q9QXL2|KI21A_MOUSE Kinesin family member 21A
Length = 1672
Score = 32.3 bits (72), Expect = 2.1
Identities = 33/136 (24%), Positives = 64/136 (47%), Gaps = 15/136 (11%)
Frame = +1
Query: 535 KSRLKNLNKGLSNENDLNKSFDISQILYFEKHESDDEFEDAMD-EIKFEDMXXXXXXXXX 711
K K +++ +NE D+ ++ ++S E+ E D+E ED ++ E ++
Sbjct: 585 KKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKAN 644
Query: 712 XXXDIDN---ELKLIANIKAELRNSQHQ---LKLQYDR--LIKRVKVSDNPLD------S 849
D+ N E+ + + EL NSQ + LK QY+ ++ + K+ D L+ +
Sbjct: 645 YQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQN 704
Query: 850 IAFASLHPESKAKQMK 897
+ + E KAK++K
Sbjct: 705 LGSVESYSEEKAKKVK 720
>sp|O33600|RAD50_SULAC DNA double-strand break repair rad50 ATPase
Length = 886
Score = 31.6 bits (70), Expect = 3.5
Identities = 63/331 (19%), Positives = 126/331 (38%), Gaps = 24/331 (7%)
Frame = +1
Query: 16 ERINEYYNQQRILINLKLDLSKNLRSLYSNFLAEMLN-DQSKYEILIFRDCVGQRMENLS 192
E + E LI + D ++L+S+ E D+ K EI + + ++EN+
Sbjct: 161 EMLRESNGPIHSLIKVLTDRIRSLQSIKDILKREEAEIDRLKKEI----EEIKVKLENIE 216
Query: 193 VLYHTENENVCHYNLMLHSADDIIVKRKLQLIHQWEHGQEMTKPIVRHEDYSLIQLKNLS 372
+ + + YN + +I V+ + +++ + +R +D+ + K +
Sbjct: 217 REAKEKEDELNQYNTEFNRIKEIKVQYDILSGELSVVNKKIEEIALRLKDFEEKE-KRYN 275
Query: 373 SANTEVSEIDQFYSYITR--AYKLLLIYYEARRAKFTNQKQIINDPINIVPPQSSAKSRL 546
TEV E+D+ I ++K +L+ ++ +++ IN+V K
Sbjct: 276 KIETEVKELDENREKINTISSFKSILVQIDSLKSQ-----------INVVENDLKRKKEK 324
Query: 547 KNLNKGLSNENDLNKSFDISQILYFEKHESDDEFE-----------------DAMDEIKF 675
K L + + + + EK + +E E + ++ +
Sbjct: 325 LKRKKELEEKEKQYEEIEKRKKELEEKEKQYEEIEKRLTYVLKNIERQKNEIEKLNYVDT 384
Query: 676 EDMXXXXXXXXXXXXDIDNELKLIANIKAELRNSQHQLKLQYDRLIK----RVKVSDNPL 843
+D+ IDNELK + + + +L + Q Y+ L R + PL
Sbjct: 385 QDLENKIKDVSDRINQIDNELKGLLDRRGDLNGRKEQTLKIYNNLNSIEDDRCPICGRPL 444
Query: 844 DSIAFASLHPESKAKQMKKPLPKPGTPLSKR 936
DS A + E K + ++ L K T L R
Sbjct: 445 DSEHKAKIREEIKVQLLE--LNKQITALQAR 473
>sp|Q9QXZ0|MACF1_MOUSE Microtubule-actin crosslinking factor 1 (Actin cross-linking family
7)
Length = 5327
Score = 31.2 bits (69), Expect = 4.6
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +1
Query: 727 DNELKLIANIKAELRNSQHQLKLQYDRLIKRVKVSDNPLDSIAFASLHPESK 882
+N+ KL LR HQ K QY+ L +R +V+ L+ ++L E++
Sbjct: 1620 ENQTKLSPQELTALREKLHQAKEQYEVLQERTRVAQKELEEAVTSALQQETE 1671
>sp|P05084|HUNB_DROME Hunchback protein
Length = 758
Score = 31.2 bits (69), Expect = 4.6
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +1
Query: 487 QIINDPINIVPPQSSAKSRLKNLNKGLSNENDLNKSFDISQILYFEKHESDD 642
Q + P+++ PP+S AKS N+ ++ N S D+ Y + E DD
Sbjct: 175 QALTPPMDVTPPKSPAKSSQSNIEPEKEHDQMSNSSEDMK---YMAESEDDD 223
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 95,729,891
Number of Sequences: 369166
Number of extensions: 1822888
Number of successful extensions: 4970
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4756
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4968
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10096222345
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)