Planarian EST Database


Dr_sW_018_D13

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_018_D13
         (838 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9UKR3|KLK13_HUMAN  Kallikrein 13 precursor (Kallikrein-l...   140   6e-33
sp|P80646|CTRB_GADMO  Chymotrypsin B [Contains: Chymotrypsin...   127   3e-29
sp|P47796|CTRA_GADMO  Chymotrypsin A precursor                    126   8e-29
sp|Q9JHJ7|TEST_MOUSE  Testisin precursor (Tryptase 4)             124   2e-28
sp|P40313|CTRL_HUMAN  Chymotrypsin-like protease CTRL-1 prec...   124   4e-28
sp|Q61955|NRPN_MOUSE  Neuropsin precursor (NP) (Kallikrein 8)     123   5e-28
sp|P26262|KLKB1_MOUSE  Plasma kallikrein precursor (Plasma p...   121   2e-27
sp|P00765|TRYP_ASTFL  Trypsin I                                   120   4e-27
sp|O88780|NRPN_RAT  Neuropsin precursor (NP) (Kallikrein-8) ...   120   4e-27
sp|Q91VE3|KLK7_MOUSE  Kallikrein-7 precursor (Stratum corneu...   118   2e-26
>sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-like protein 4) (KLK-L4)
          Length = 277

 Score =  140 bits (352), Expect = 6e-33
 Identities = 88/249 (35%), Positives = 125/249 (50%), Gaps = 1/249 (0%)
 Frame = +1

Query: 37  TSGWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFK 216
           TSG+ +  G     H  P   ++ VQG   CGG L++   VLTAAHC+ +          
Sbjct: 32  TSGF-LPGGYTCFPHSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCLKE---------- 80

Query: 217 YYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLE-GNDVALIKFDGYISKNQ 393
              +KVY+G++ L R+   E++ +V  +I HP YR    +L   +D+ L++    +    
Sbjct: 81  --GLKVYLGKHALGRVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPVQLT- 137

Query: 394 YDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNV 573
             YI    L  N    PG  C V GWG+   P  +  + LQ A I + S+ EC +++   
Sbjct: 138 -GYIQTLPLSHNNRLTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEECRQVYPG- 195

Query: 574 AYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTR 753
               + +CAG+      SC GDSGGPL C     +    GIVS+G  PCGQP+ P VYTR
Sbjct: 196 KITDNMLCAGTKEGGKDSCEGDSGGPLVC-----NRTLYGIVSWGDFPCGQPDRPGVYTR 250

Query: 754 VSEVAGWIR 780
           VS    WIR
Sbjct: 251 VSRYVLWIR 259
>sp|P80646|CTRB_GADMO Chymotrypsin B [Contains: Chymotrypsin B chain A; Chymotrypsin B
           chain B]
          Length = 245

 Score =  127 bits (320), Expect = 3e-29
 Identities = 89/251 (35%), Positives = 127/251 (50%), Gaps = 3/251 (1%)
 Frame = +1

Query: 34  VTSGWKIINGSQAIMHEYPQMVSIKVQ-GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNT 210
           VT   +I+NG +A+ H +P  VS++   G HFCGG+L+N N V+TAAHC       NV T
Sbjct: 10  VTGYARIVNGEEAVPHSWPWQVSLQQSNGFHFCGGSLINENWVVTAAHC-------NVRT 62

Query: 211 FKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKF--DGYIS 384
           +     +V +GE+D      ++ I + +    HP +         ND++LIK      + 
Sbjct: 63  YH----RVIVGEHDKASDENIQ-ILKPSMVFTHPKWDSRTI---NNDISLIKLASPAVLG 114

Query: 385 KNQYDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIF 564
            N          DV A   PG +C   GWG        T   LQ+AA+ ++SN EC + +
Sbjct: 115 TNVSPVCLGESSDVFA---PGMKCVTSGWGLTRYNAPGTPNKLQQAALPLMSNEECSQTW 171

Query: 565 SNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSV 744
            N       ICAG+     +SC GDSGGPL C       + VGIVS+G+  C     P+V
Sbjct: 172 GNNMISDVMICAGAA--GATSCMGDSGGPLVCQKDNVWTL-VGIVSWGSSRC-SVTTPAV 227

Query: 745 YTRVSEVAGWI 777
           Y RV+E+ GW+
Sbjct: 228 YARVTELRGWV 238
>sp|P47796|CTRA_GADMO Chymotrypsin A precursor
          Length = 263

 Score =  126 bits (316), Expect = 8e-29
 Identities = 82/250 (32%), Positives = 131/250 (52%), Gaps = 2/250 (0%)
 Frame = +1

Query: 34  VTSGWKIINGSQAIMHEYPQMVSIKVQ-GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNT 210
           +T   +I+NG +A+ H +   VS++ Q G HFCGG+L+N N V+TAAHC  +++N++   
Sbjct: 28  ITGYSRIVNGEEAVPHSWSWQVSLQDQTGFHFCGGSLINENWVVTAAHC--NVKNYH--- 82

Query: 211 FKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKN 390
                 +V +GE+D        ++  V +   HP Y     +   ND+ L+K     + N
Sbjct: 83  ------RVVLGEHDRSSNSEGVQVMTVGQVFKHPRYN---GFTINNDILLVKLATPATLN 133

Query: 391 -QYDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFS 567
            +   +   E D   + + G +C   GWG       DT   LQ+AA+ +++N +C + + 
Sbjct: 134 MRVSPVCLAETD--DVFEGGMKCVTSGWGLTRYNAADTPALLQQAALPLLTNEQCKKFWG 191

Query: 568 NVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVY 747
           N     S++   +G    SSC GDSGGPL C       + VGIVS+G+  C  P +P VY
Sbjct: 192 NKI---SDLMICAGAAGASSCMGDSGGPLVCQKAGSWTL-VGIVSWGSGTC-TPTMPGVY 246

Query: 748 TRVSEVAGWI 777
            RV+E+  W+
Sbjct: 247 ARVTELRAWV 256
>sp|Q9JHJ7|TEST_MOUSE Testisin precursor (Tryptase 4)
          Length = 324

 Score =  124 bits (312), Expect = 2e-28
 Identities = 90/259 (34%), Positives = 131/259 (50%), Gaps = 14/259 (5%)
 Frame = +1

Query: 49  KIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVW---DMRNWNVNTFKY 219
           +I+ G  A +  +P   S++V GNH CG TLLN   VLTAAHC     D  +W V     
Sbjct: 54  RIVGGDDAELGRWPWQGSLRVWGNHLCGATLLNRRWVLTAAHCFQKDNDPFDWTV----- 108

Query: 220 YQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYD 399
            Q         L+ +      +Q+    + P Y  +Y     ND+AL+K    ++ N  +
Sbjct: 109 -QFGELTSRPSLWNLQAYSNRYQIEDIFLSPKYSEQYP----NDIALLKLSSPVTYN--N 161

Query: 400 YIDYNELDVNAISKPGDR--CEVVGWGSMLPPGQDTS----RFLQKAAINIISNSECGRI 561
           +I    L +N+  K  +R  C V GWG++   G+D S      LQ+  + II+NS C  +
Sbjct: 162 FIQPICL-LNSTYKFENRTDCWVTGWGAI---GEDESLPSPNTLQEVQVAIINNSMCNHM 217

Query: 562 FSNVAYVRSNI-----CAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQ 726
           +    + R+NI     CAG+      +C GDSGGPL C D      QVG+VS+G + CG+
Sbjct: 218 YKKPDF-RTNIWGDMVCAGTPEGGKDACFGDSGGPLAC-DQDTVWYQVGVVSWG-IGCGR 274

Query: 727 PNVPSVYTRVSEVAGWIRA 783
           PN P VYT +S    WI++
Sbjct: 275 PNRPGVYTNISHHYNWIQS 293
>sp|P40313|CTRL_HUMAN Chymotrypsin-like protease CTRL-1 precursor
          Length = 264

 Score =  124 bits (310), Expect = 4e-28
 Identities = 87/252 (34%), Positives = 130/252 (51%), Gaps = 3/252 (1%)
 Frame = +1

Query: 31  AVTSGWKIINGSQAIMHEYPQMVSIK-VQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVN 207
           A++   +I+NG  A++  +P  VS++   G HFCGG+L++ + V+TAAHC       NV+
Sbjct: 27  ALSFSQRIVNGENAVLGSWPWQVSLQDSSGFHFCGGSLISQSWVVTAAHC-------NVS 79

Query: 208 TFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISK 387
             +++   V +GEYD        ++  V++ I HPS+    +    NDV L+K     S 
Sbjct: 80  PGRHF---VVLGEYDRSSNAEPLQVLSVSRAITHPSWN---STTMNNDVTLLKL---ASP 130

Query: 388 NQYD--YIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRI 561
            QY            N     G  C   GWG +   G  T   LQ+ A+ +++ ++C R 
Sbjct: 131 AQYTTRISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVTPAHLQQVALPLVTVNQC-RQ 189

Query: 562 FSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPS 741
           +   +   S ICAG      SSC+GDSGGPL C  G    + +GIVS+GT  C     P+
Sbjct: 190 YWGSSITDSMICAGGA--GASSCQGDSGGPLVCQKGNT-WVLIGIVSWGTKNC-NVRAPA 245

Query: 742 VYTRVSEVAGWI 777
           VYTRVS+ + WI
Sbjct: 246 VYTRVSKFSTWI 257
>sp|Q61955|NRPN_MOUSE Neuropsin precursor (NP) (Kallikrein 8)
          Length = 260

 Score =  123 bits (309), Expect = 5e-28
 Identities = 79/246 (32%), Positives = 116/246 (47%)
 Frame = +1

Query: 43  GWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKYY 222
           G KI+ G + I H  P   ++       CGG L+    VLTAAHC            K  
Sbjct: 30  GSKILEGRECIPHSQPWQAALFQGERLICGGVLVGDRWVLTAAHC------------KKQ 77

Query: 223 QMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDY 402
           +  V +G++ L      E+  QV ++I HP Y +       +D+ LI+     S N  D 
Sbjct: 78  KYSVRLGDHSLQSRDQPEQEIQVAQSIQHPCYNNSNPEDHSHDIMLIRLQN--SANLGDK 135

Query: 403 IDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYV 582
           +   +L  N   K G +C + GWG++  P ++    L  A + I S ++C R +      
Sbjct: 136 VKPVQL-ANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERAYPG-KIT 193

Query: 583 RSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSE 762
              +CAGS      +C+GDSGGPL C DG       GI S+G+ PCG+P  P VYT++  
Sbjct: 194 EGMVCAGSSN-GADTCQGDSGGPLVC-DGMLQ----GITSWGSDPCGKPEKPGVYTKICR 247

Query: 763 VAGWIR 780
              WI+
Sbjct: 248 YTTWIK 253
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
            (Fletcher factor) [Contains: Plasma kallikrein heavy
            chain; Plasma kallikrein light chain]
          Length = 638

 Score =  121 bits (304), Expect = 2e-27
 Identities = 85/256 (33%), Positives = 126/256 (49%), Gaps = 6/256 (2%)
 Frame = +1

Query: 28   CAVTSGWKIINGSQAIMHEYPQMVSIKVQ---GNHFCGGTLLNGNTVLTAAHCVWDMRNW 198
            C      +I+ G+ A + E+P  VS++V+     H CGG+++    VLTAAHC   +   
Sbjct: 383  CTTKINARIVGGTNASLGEWPWQVSLQVKLVSQTHLCGGSIIGRQWVLTAAHCFDGIPYP 442

Query: 199  NVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGN-DVALIKFDG 375
            +V        ++Y G   L  +       ++ + IIH     EY   EGN D+ALIK   
Sbjct: 443  DV-------WRIYGGILSLSEITKETPSSRIKELIIH----QEYKVSEGNYDIALIKLQT 491

Query: 376  YISKNQYD--YIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSE 549
             ++  ++       ++ D N I      C V GWG     G+ T   LQKA I ++ N E
Sbjct: 492  PLNYTEFQKPICLPSKADTNTIYT---NCWVTGWGYTKEQGE-TQNILQKATIPLVPNEE 547

Query: 550  CGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQP 729
            C + + +    +  ICAG     T +C+GDSGGPL C    +  + VGI S+G   CG+ 
Sbjct: 548  CQKKYRDYVINKQMICAGYKEGGTDACKGDSGGPLVCKHSGRWQL-VGITSWGE-GCGRK 605

Query: 730  NVPSVYTRVSEVAGWI 777
            + P VYT+VSE   WI
Sbjct: 606  DQPGVYTKVSEYMDWI 621
>sp|P00765|TRYP_ASTFL Trypsin I
          Length = 237

 Score =  120 bits (302), Expect = 4e-27
 Identities = 82/254 (32%), Positives = 127/254 (50%), Gaps = 8/254 (3%)
 Frame = +1

Query: 52  IINGSQAIMHEYPQMVSIKVQ----GNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKY 219
           I+ G+ A++ E+P  +S +        HFCG ++ N N  +TA HCV+     N +    
Sbjct: 1   IVGGTDAVLGEFPYQLSFQETFLGFSFHFCGASIYNENYAITAGHCVYGDDYENPSG--- 57

Query: 220 YQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYD 399
             +++  GE D+    G E+   V+K I+H ++ ++   L  ND++L+K  G ++ N   
Sbjct: 58  --LQIVAGELDMSVNEGSEQTITVSKIILHENFDYD---LLDNDISLLKLSGSLTFN--- 109

Query: 400 YIDYNELDVNAISKPGDRCE----VVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFS 567
               N +   A+   G        V GWG+    G +T   LQK  + ++S++EC   + 
Sbjct: 110 ----NNVAPIALPAQGHTATGNVIVTGWGTT-SEGGNTPDVLQKVTVPLVSDAECRDDYG 164

Query: 568 NVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVY 747
                 S ICAG       SC+GDSGGPL  +D    ++  GIVS+G   C +P  P VY
Sbjct: 165 ADEIFDSMICAGVPEGGKDSCQGDSGGPLAASDTGSTYL-AGIVSWG-YGCARPGYPGVY 222

Query: 748 TRVSEVAGWIRANA 789
           T VS    WI+ANA
Sbjct: 223 TEVSYHVDWIKANA 236
>sp|O88780|NRPN_RAT Neuropsin precursor (NP) (Kallikrein-8) (Brain serine protease 1)
          Length = 260

 Score =  120 bits (302), Expect = 4e-27
 Identities = 77/246 (31%), Positives = 115/246 (46%)
 Frame = +1

Query: 43  GWKIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAHCVWDMRNWNVNTFKYY 222
           G KI+ G +   H  P   ++       CGG L+    VLTAAHC  D         KY 
Sbjct: 30  GSKILEGQECKPHSQPWQTALFQGERLVCGGVLVGDRWVLTAAHCKKD---------KY- 79

Query: 223 QMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDVALIKFDGYISKNQYDY 402
              V +G++ L +    E+  QV ++I HP +         +D+ LI+     S N  D 
Sbjct: 80  --SVRLGDHSLQKRDEPEQEIQVARSIQHPCFNSSNPEDHSHDIMLIRLQN--SANLGDK 135

Query: 403 IDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINIISNSECGRIFSNVAYV 582
           +   EL  N   K G +C + GWG++  P ++    L  A + I S ++C R +      
Sbjct: 136 VKPIEL-ANLCPKVGQKCIISGWGTVTSPQENFPNTLNCAEVKIYSQNKCERAYPG-KIT 193

Query: 583 RSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTVPCGQPNVPSVYTRVSE 762
              +CAGS      +C+GDSGGPL C       +  GI ++G+ PCG+P  P VYT++  
Sbjct: 194 EGMVCAGSSN-GADTCQGDSGGPLVCNG-----VLQGITTWGSDPCGKPEKPGVYTKICR 247

Query: 763 VAGWIR 780
              WI+
Sbjct: 248 YTNWIK 253
>sp|Q91VE3|KLK7_MOUSE Kallikrein-7 precursor (Stratum corneum chymotryptic enzyme)
           (Thymopsin)
          Length = 249

 Score =  118 bits (296), Expect = 2e-26
 Identities = 82/261 (31%), Positives = 130/261 (49%), Gaps = 2/261 (0%)
 Frame = +1

Query: 1   SFLFVLITYCAVTSGW--KIINGSQAIMHEYPQMVSIKVQGNHFCGGTLLNGNTVLTAAH 174
           S + VL++    T+G   +II+G +     +P  V++       CGG L++   VLTAAH
Sbjct: 7   SLITVLLSLALETAGQGERIIDGYKCKEGSHPWQVALLKGNQLHCGGVLVDKYWVLTAAH 66

Query: 175 CVWDMRNWNVNTFKYYQMKVYMGEYDLYRMYGVEKIFQVTKTIIHPSYRHEYAYLEGNDV 354
           C            K  Q +V +G  D       +KI + TK+  HP Y  +      ND+
Sbjct: 67  C------------KMGQYQVQLGS-DKIGDQSAQKI-KATKSFRHPGYSTK---THVNDI 109

Query: 355 ALIKFDGYISKNQYDYIDYNELDVNAISKPGDRCEVVGWGSMLPPGQDTSRFLQKAAINI 534
            L++ D  +  +    ++  +L  +    PG  C V GWG+   P       L  + + +
Sbjct: 110 MLVRLDEPVKMSSK--VEAVQLPEHC-EPPGTSCTVSGWGTTTSPDVTFPSDLMCSDVKL 166

Query: 535 ISNSECGRIFSNVAYVRSNICAGSGRLSTSSCRGDSGGPLFCTDGRKHHIQVGIVSYGTV 714
           IS+ EC +++ ++   ++ +CAG     T++C GDSGGPL C D  +     G+VS+GT 
Sbjct: 167 ISSRECKKVYKDLLG-KTMLCAGIPDSKTNTCNGDSGGPLVCNDTLQ-----GLVSWGTY 220

Query: 715 PCGQPNVPSVYTRVSEVAGWI 777
           PCGQPN P VYT+V +   W+
Sbjct: 221 PCGQPNDPGVYTQVCKYKRWV 241
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 104,825,908
Number of Sequences: 369166
Number of extensions: 2323109
Number of successful extensions: 7201
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5863
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6100
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8148988185
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)