Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_C14
(953 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-r... 47 8e-05
sp|Q99541|ADFP_HUMAN Adipophilin (Adipose differentiation-r... 38 0.048
sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding... 35 0.24
sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-r... 34 0.69
sp|P19269|AMY1_DEBOC Alpha-amylase 1 precursor (1,4-alpha-D... 32 2.0
sp|Q00496|BXE_CLOBO Botulinum neurotoxin type E precursor (... 32 3.4
sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2) 32 3.4
sp|P51818|HSP83_ARATH Heat shock protein 81-3 (HSP81-3) (HS... 32 3.4
sp|P57498|CYSN_BUCAI Sulfate adenylyltransferase subunit 1 ... 32 3.4
sp|Q32065|YCX9_CHLRE Hypothetical 341.7 kDa protein in psbD... 32 3.4
>sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 425
Score = 47.0 bits (110), Expect = 8e-05
Identities = 62/317 (19%), Positives = 125/317 (39%), Gaps = 19/317 (5%)
Frame = +1
Query: 16 RIREYPVIKDTFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS------- 174
R+ P++ T+ Y K++ L S E +K V A
Sbjct: 15 RVANLPLVSSTYDLVSSAYVSTKDQYPYLRSVCEMAEKGVKTVTSAAMTSALPIIQKLEP 74
Query: 175 KLGSVDNFACNQLDKLETKYPXXXXXXXXXXXXXGEYYDSSIKPNVDKMIAAKDSAVYIA 354
++ + +AC LD++E + P + +++A+ AV A
Sbjct: 75 QIAVANTYACKGLDRMEERLPI-------------------LNQPTSEIVASARGAVTGA 115
Query: 355 KTPFRITGQTIEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFT 534
K + T+ ++ S V + K K A G+VE+ + +++ +
Sbjct: 116 KD---VVTTTMAGAKDSVASTVSGVVDKTKGAVTGSVERTK-------------SVVNGS 159
Query: 535 LKNIEGLSNVLNSIYDQSVTKNKEWLHGYY----EEFTDEMKKNSGDESKTESTVMQLAQ 702
+ + G+ +NS D ++TK++ + Y+ EE E KK G + + + + +
Sbjct: 160 INTVLGMVQFMNSGVDNAITKSEMLVDQYFPLTQEELEMEAKKVEGFDMVQKPSNYERLE 219
Query: 703 NFSNKL--RVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTY------QEIR 858
+ S KL R +A+S V ++ET+ + T H+ K+ + Q +
Sbjct: 220 SLSTKLCSRAYHQALSRV--KEAKQKSQETIS-QLHSTVHLIEFARKNMHSANQKIQGAQ 276
Query: 859 DQISIENIKWIGQNSIG 909
D++ + ++W + SIG
Sbjct: 277 DKLYVSWVEW--KRSIG 291
>sp|Q99541|ADFP_HUMAN Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 437
Score = 37.7 bits (86), Expect = 0.048
Identities = 61/313 (19%), Positives = 122/313 (38%), Gaps = 15/313 (4%)
Frame = +1
Query: 16 RIREYPVIKDTFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS------- 174
R+ P++ T+ Y K++ L S E+ +K + + A
Sbjct: 15 RVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQKLEP 74
Query: 175 KLGSVDNFACNQLDKLETKYPXXXXXXXXXXXXXGEYYDSSIKPNVDKMIAAKDSAVYIA 354
++ + +AC LD++E + P + +++A AV A
Sbjct: 75 QIAVANTYACKGLDRIEERLPI-------------------LNQPSTQIVANAKGAVTGA 115
Query: 355 KTPFRITGQTIEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFT 534
K T+ ++ S + M K K A G+VEK + + +NT+L
Sbjct: 116 KD---AVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVV-----SGSINTVLGSR 167
Query: 535 LKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTESTVMQLAQNFSN 714
+ + +S+ + + +S +++L EE E KK G + + + + S
Sbjct: 168 MMQL--VSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLST 225
Query: 715 KL--RVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTY------QEIRDQIS 870
KL R +A+S V +++T+ + T H+ K+ Y Q+ +D++
Sbjct: 226 KLHSRAYQQALSRV--KEAKQKSQQTIS-QLHSTVHLIEFARKNVYSANQKIQDAQDKLY 282
Query: 871 IENIKWIGQNSIG 909
+ ++W + SIG
Sbjct: 283 LSWVEW--KRSIG 293
>sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding protein 2 (Lsd2)
Length = 352
Score = 35.4 bits (80), Expect = 0.24
Identities = 24/82 (29%), Positives = 35/82 (42%), Gaps = 3/82 (3%)
Frame = +1
Query: 1 IKSLERIREYPVIKDTFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS-- 174
++SLERI + PV+ + K+ + Y +K KN LT E + P
Sbjct: 37 LESLERIIKLPVVNAAWDKSQDVYGKVKGKNRVFEWALTAAEDCVTRAVTTAAPFVTKLD 96
Query: 175 -KLGSVDNFACNQLDKLETKYP 237
+ VD +DKLE K P
Sbjct: 97 RPIAYVDQTLVKGIDKLEVKAP 118
>sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 450
Score = 33.9 bits (76), Expect = 0.69
Identities = 58/315 (18%), Positives = 115/315 (36%), Gaps = 17/315 (5%)
Frame = +1
Query: 16 RIREYPVIKDTFTKAGEYYTWIKEKNSKLNSTLTWTESNLKIVKDMVEPIAAS------- 174
R+ P++ T+ Y K++ L S E +K + + A
Sbjct: 15 RVANLPLVSSTYDLVSSAYISRKDQYPYLKSLCEMAEKGMKTITSVAVTSALPIIQKLEP 74
Query: 175 KLGSVDNFACNQLDKLETKYPXXXXXXXXXXXXXGEYYDSSIKPNVDKMIAAKDSAVYIA 354
++ + +AC LD++E K P + ++++A A+ A
Sbjct: 75 QIAVANTYACKGLDRIEEKLPI-------------------LNQPTNQVVANAKGAMTGA 115
Query: 355 KTPFRITGQTIEFLSNTTNSFVHRFMSKDKKAEYGAVEKKEYDILYRRALKEVNTILTFT 534
K T+ ++ S + + + K A G+VEK + + +NT+L
Sbjct: 116 KD---AVTTTVTGAKDSVASTITGVVDRTKGAVTGSVEKTKSVV-----SGSINTVLRSR 167
Query: 535 LKNI--EGLSNVLNS---IYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTESTVMQLA 699
+ + G+ N L + DQ + K +E E KK G + + +
Sbjct: 168 VMQLMSSGVENALTKSELLVDQYLPLTK-------DELEKEAKKVEGFDMVQKPSYYVRL 220
Query: 700 QNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLM---FNKTEHIYSTL--SKSTYQEIRDQ 864
+ S KLR + +ET+ + FN +E + + Q+ +D+
Sbjct: 221 GSLSTKLRSRAYQQALCRVEEAKRKGQETISQLHSAFNLSELARKNVHNANQKIQDAQDK 280
Query: 865 ISIENIKWIGQNSIG 909
+ + ++W + SIG
Sbjct: 281 LYLSWLEW--KRSIG 293
>sp|P19269|AMY1_DEBOC Alpha-amylase 1 precursor (1,4-alpha-D-glucan glucanohydrolase 1)
Length = 512
Score = 32.3 bits (72), Expect = 2.0
Identities = 28/105 (26%), Positives = 48/105 (45%), Gaps = 5/105 (4%)
Frame = +1
Query: 613 HGYYEEFTDEMKKNSGDESKTESTVMQLAQNFSNKLRVSVRAMSTVCYNHMS-SGNKETL 789
HGY+ + DE+ N G T + QLA ++ S+ M V YNH + +G+ ++
Sbjct: 116 HGYWMKNIDELNTNFG----TADELKQLASELHSR---SMLLMVDVVYNHYAWNGDGSSV 168
Query: 790 DLM----FNKTEHIYSTLSKSTYQEIRDQISIENIKWIGQNSIGL 912
D FN+ + + + Y DQ ++E+ W G + L
Sbjct: 169 DYSSFTPFNQQSYFHDYCLITNY---NDQTNVEDC-WEGDTEVSL 209
>sp|Q00496|BXE_CLOBO Botulinum neurotoxin type E precursor (BoNT/E) (Bontoxilysin E)
[Contains: Botulinum neurotoxin E light chain; Botulinum
neurotoxin E heavy chain]
Length = 1251
Score = 31.6 bits (70), Expect = 3.4
Identities = 35/145 (24%), Positives = 67/145 (46%), Gaps = 7/145 (4%)
Frame = +1
Query: 511 VNTILTFTLKNIEGLSN----VLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTE 678
+ TIL FT+K+ G S+ V+ +I + ++++W Y ++ M K + +K +
Sbjct: 642 IPTILVFTIKSFLGSSDNKNKVIKAINNALKERDEKWKEVYSFIVSNWMTKINTQFNKRK 701
Query: 679 STVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTYQEIR 858
+ Q QN V A+ T+ + +S E + + NK + K E+
Sbjct: 702 EQMYQALQN-------QVNAIKTIIESKYNSYTLEEKNELTNKYD------IKQIENELN 748
Query: 859 DQISI--ENI-KWIGQNSIGLLSNI 924
++SI NI +++ ++SI L I
Sbjct: 749 QKVSIAMNNIDRFLTESSISYLMKI 773
>sp|P55737|HSP82_ARATH Heat shock protein 81-2 (HSP81-2)
Length = 699
Score = 31.6 bits (70), Expect = 3.4
Identities = 20/87 (22%), Positives = 42/87 (48%)
Frame = +1
Query: 457 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 636
G V+ ++ + R + N IL KN+ + L ++ + +NKE + +YE F+
Sbjct: 358 GIVDSEDLPLNISRETLQQNKILKVIRKNL--VKKCLELFFE--IAENKEDYNKFYEAFS 413
Query: 637 DEMKKNSGDESKTESTVMQLAQNFSNK 717
+K ++S+ + + +L + S K
Sbjct: 414 KNLKLGIHEDSQNRTKIAELLRYHSTK 440
>sp|P51818|HSP83_ARATH Heat shock protein 81-3 (HSP81-3) (HSP81.2)
Length = 699
Score = 31.6 bits (70), Expect = 3.4
Identities = 20/87 (22%), Positives = 42/87 (48%)
Frame = +1
Query: 457 GAVEKKEYDILYRRALKEVNTILTFTLKNIEGLSNVLNSIYDQSVTKNKEWLHGYYEEFT 636
G V+ ++ + R + N IL KN+ + L ++ + +NKE + +YE F+
Sbjct: 358 GIVDSEDLPLNISRETLQQNKILKVIRKNL--VKKCLELFFE--IAENKEDYNKFYEAFS 413
Query: 637 DEMKKNSGDESKTESTVMQLAQNFSNK 717
+K ++S+ + + +L + S K
Sbjct: 414 KNLKLGIHEDSQNRTKIAELLRYHSTK 440
>sp|P57498|CYSN_BUCAI Sulfate adenylyltransferase subunit 1 (Sulfate adenylate
transferase) (SAT) (ATP-sulfurylase large subunit)
Length = 473
Score = 31.6 bits (70), Expect = 3.4
Identities = 42/160 (26%), Positives = 73/160 (45%), Gaps = 15/160 (9%)
Frame = +1
Query: 376 GQTIEFLSNTTNSFVHRFMSKD---KKAEYG----AVEKKEYDILYRRALKEVNTILTFT 534
GQTI+ L NS V R ++ D KKAE G V K E DI +++IL +
Sbjct: 269 GQTIKILPENINSRVSRIVTFDKELKKAEIGESITVVLKDEIDINRGDFFVNIDSILQPS 328
Query: 535 LKNIEGL----SNVLNSIYDQSVTKNKEWLHGYYEEFTDEMKKNSGDESKTESTVMQLAQ 702
+ I + N+L + +V + + + Y +E ++ N+ + K+ S V+
Sbjct: 329 QEAIIDIVWMTDNILLAGESYNVKLSGKKIRVYIKEILFKLDVNTLKKVKSHSLVL---- 384
Query: 703 NFSNKLRVSVRAMSTVCYNHMS----SGNKETLDLMFNKT 810
++ RV + V +++ S +GN +DL+ N T
Sbjct: 385 --NSIGRVKIYFSKPVIFDNYSENRMTGNMIFIDLLTNIT 422
>sp|Q32065|YCX9_CHLRE Hypothetical 341.7 kDa protein in psbD-psbC intergenic region
(ORF2971) (ORFB)
Length = 2971
Score = 31.6 bits (70), Expect = 3.4
Identities = 25/91 (27%), Positives = 45/91 (49%), Gaps = 2/91 (2%)
Frame = +1
Query: 676 ESTVMQLAQNFSNKLRVSVRAMSTVCYNHMSSGNKETLDLMFNKTEHIYSTLSKSTYQEI 855
+S ++A +F N L +S++ YN++S NK L K ++S + + +
Sbjct: 435 DSESQEVATSFQNDL-ISIKYCFNNLYNYIS--NKTALS---TKNLFLFSAIKSNATKHK 488
Query: 856 RDQ--ISIENIKWIGQNSIGLLSNIKESLSA 942
R Q S+EN +G NS + + K S++A
Sbjct: 489 RTQSFFSVENTTTLGNNSNFVKGHFKSSINA 519
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.312 0.127 0.352
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,286,457
Number of Sequences: 369166
Number of extensions: 2121952
Number of successful extensions: 4588
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4384
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4586
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 9856975370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)