Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_P20
(380 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P00940|TPIS_CHICK Triosephosphate isomerase (TIM) (Trios... 130 7e-31
sp|Q589R5|TPIS_ORYLA Triosephosphate isomerase (TIM) (Trios... 127 8e-30
sp|P48501|TPIS_SCHMA Triosephosphate isomerase (TIM) (Trios... 120 1e-27
sp|P54714|TPIS_CANFA Triosephosphate isomerase (TIM) (Trios... 118 4e-27
sp|P00941|TPIS_LATCH Triosephosphate isomerase (TIM) (Trios... 117 8e-27
sp|Q60HC9|TPIS_MACFA Triosephosphate isomerase (TIM) (Trios... 117 1e-26
sp|P60174|TPIS_HUMAN Triosephosphate isomerase (TIM) (Trios... 117 1e-26
sp|Q27775|TPIS_SCHJA Triosephosphate isomerase (TIM) (Trios... 116 2e-26
sp|P00939|TPIS_RABIT Triosephosphate isomerase (TIM) (Trios... 115 4e-26
sp|P48500|TPIS_RAT Triosephosphate isomerase (TIM) (Triose-... 115 4e-26
>sp|P00940|TPIS_CHICK Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 130 bits (328), Expect = 7e-31
Identities = 65/90 (72%), Positives = 71/90 (78%)
Frame = -3
Query: 324 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 145
RKFFVGGNWKMNGDK L ++ ++L AKL TEVV PSIYLDF RQKL AKIGV+A
Sbjct: 4 RKFFVGGNWKMNGDKKSLGELIHTLNGAKLSADTEVVCGAPSIYLDFARQKLDAKIGVAA 63
Query: 144 QNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
QN YK+PKGAFTGEISPAMIKD WVIL
Sbjct: 64 QNCYKVPKGAFTGEISPAMIKDIGAAWVIL 93
Score = 31.6 bits (70), Expect = 0.59
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +2
Query: 14 EIPKGAFTGEISPARI 61
++PKGAFTGEISPA I
Sbjct: 68 KVPKGAFTGEISPAMI 83
>sp|Q589R5|TPIS_ORYLA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 127 bits (319), Expect = 8e-30
Identities = 61/91 (67%), Positives = 72/91 (79%)
Frame = -3
Query: 327 QRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVS 148
QR+FFVGGNWKMNG+K+ L ++ ++L A LHE TEVV A PSIYLDF R L ++I V+
Sbjct: 3 QRRFFVGGNWKMNGNKESLEELMSTLNTASLHEDTEVVCAAPSIYLDFARSGLDSRISVA 62
Query: 147 AQNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
AQN YK+ KGAFTGEISPAMIKD DWVIL
Sbjct: 63 AQNCYKVAKGAFTGEISPAMIKDCGADWVIL 93
Score = 28.5 bits (62), Expect = 5.0
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +2
Query: 14 EIPKGAFTGEISPARI 61
++ KGAFTGEISPA I
Sbjct: 68 KVAKGAFTGEISPAMI 83
>sp|P48501|TPIS_SCHMA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 253
Score = 120 bits (301), Expect = 1e-27
Identities = 56/94 (59%), Positives = 70/94 (74%)
Frame = -3
Query: 336 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 157
MS RKFFVGGNWKMNG +D+ K+ L A + TEV++APPS++L +R+ L +I
Sbjct: 1 MSGSRKFFVGGNWKMNGSRDDNDKLLKLLSEAHFDDNTEVLIAPPSVFLHEIRKSLKKEI 60
Query: 156 GVSAQNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
V+AQN YK+ KGAFTGEISPAMI+D CDWVIL
Sbjct: 61 HVAAQNCYKVSKGAFTGEISPAMIRDIGCDWVIL 94
Score = 28.5 bits (62), Expect = 5.0
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +2
Query: 14 EIPKGAFTGEISPARI 61
++ KGAFTGEISPA I
Sbjct: 69 KVSKGAFTGEISPAMI 84
>sp|P54714|TPIS_CANFA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 118 bits (296), Expect = 4e-27
Identities = 58/94 (61%), Positives = 68/94 (72%)
Frame = -3
Query: 336 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 157
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL AKI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKKNLGELITTLNAAKVPADTEVVCAPPTAYIDFARQKLDAKI 60
Query: 156 GVSAQNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
V+AQN YK+ GAFTGEISP MIKD WV+L
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVL 94
>sp|P00941|TPIS_LATCH Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 247
Score = 117 bits (293), Expect = 8e-27
Identities = 58/90 (64%), Positives = 66/90 (73%)
Frame = -3
Query: 324 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 145
RKFFVGGNWKMNGDK L ++ +L AK+ T E+V APP YLDF R K+ K GV+A
Sbjct: 3 RKFFVGGNWKMNGDKKSLGELIQTLNAAKVPFTGEIVCAPPEAYLDFARLKVDPKFGVAA 62
Query: 144 QNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
QN YK+ KGAFTGEISPAMIKD WVIL
Sbjct: 63 QNCYKVSKGAFTGEISPAMIKDCGVTWVIL 92
Score = 29.3 bits (64), Expect = 2.9
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +2
Query: 14 EIPKGAFTGEISPARITQSHILSLII 91
++ KGAFTGEISPA I + +I+
Sbjct: 67 KVSKGAFTGEISPAMIKDCGVTWVIL 92
>sp|Q60HC9|TPIS_MACFA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
sp|P15426|TPIS_MACMU Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 117 bits (292), Expect = 1e-26
Identities = 57/94 (60%), Positives = 67/94 (71%)
Frame = -3
Query: 336 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 157
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKQNLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 156 GVSAQNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
V+AQN YK+ GAFTGEISP MIKD WV+L
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVL 94
>sp|P60174|TPIS_HUMAN Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
sp|P60175|TPIS_PANTR Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 117 bits (292), Expect = 1e-26
Identities = 57/94 (60%), Positives = 67/94 (71%)
Frame = -3
Query: 336 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 157
M+ RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 156 GVSAQNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
V+AQN YK+ GAFTGEISP MIKD WV+L
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVL 94
>sp|Q27775|TPIS_SCHJA Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 252
Score = 116 bits (290), Expect = 2e-26
Identities = 56/94 (59%), Positives = 69/94 (73%)
Frame = -3
Query: 336 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 157
MS RKFFVGGNWKMNG + + + +SL A TE+++A P +YL+ VRQ L +I
Sbjct: 1 MSGSRKFFVGGNWKMNGSRADNKVLIHSLSEAHFCGDTEILIAAPFVYLNEVRQSLAKEI 60
Query: 156 GVSAQNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
V+AQN YK+PKGAFTGEISP+MIKD CDWVIL
Sbjct: 61 HVAAQNCYKVPKGAFTGEISPSMIKDVGCDWVIL 94
Score = 30.4 bits (67), Expect = 1.3
Identities = 12/16 (75%), Positives = 15/16 (93%)
Frame = +2
Query: 14 EIPKGAFTGEISPARI 61
++PKGAFTGEISP+ I
Sbjct: 69 KVPKGAFTGEISPSMI 84
>sp|P00939|TPIS_RABIT Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 248
Score = 115 bits (287), Expect = 4e-26
Identities = 56/90 (62%), Positives = 65/90 (72%)
Frame = -3
Query: 324 RKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKIGVSA 145
RKFFVGGNWKMNG K L ++ +L AK+ TEVV APP+ Y+DF RQKL KI V+A
Sbjct: 4 RKFFVGGNWKMNGRKKNLGELITTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAA 63
Query: 144 QNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
QN YK+ GAFTGEISP MIKD WV+L
Sbjct: 64 QNCYKVTNGAFTGEISPGMIKDCGATWVVL 93
>sp|P48500|TPIS_RAT Triosephosphate isomerase (TIM) (Triose-phosphate isomerase)
Length = 249
Score = 115 bits (287), Expect = 4e-26
Identities = 58/94 (61%), Positives = 67/94 (71%)
Frame = -3
Query: 336 MSNQRKFFVGGNWKMNGDKDELTKICNSLMNAKLHETTEVVVAPPSIYLDFVRQKLPAKI 157
M+ RKFFVGGNWKMNG K L ++ +L AKL TEVV APP+ Y+DF RQKL KI
Sbjct: 1 MAPSRKFFVGGNWKMNGRKKCLGELICTLNAAKLPADTEVVCAPPTAYIDFARQKLDPKI 60
Query: 156 GVSAQNSYKIPKGAFTGEISPAMIKDNMCDWVIL 55
V+AQN YK+ GAFTGEISP MIKD WV+L
Sbjct: 61 AVAAQNCYKVTNGAFTGEISPGMIKDLGATWVVL 94
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,950,402
Number of Sequences: 369166
Number of extensions: 765198
Number of successful extensions: 2687
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2403
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2552
length of database: 68,354,980
effective HSP length: 93
effective length of database: 51,174,625
effective search space used: 1688762625
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)