Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_P07
(696 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q07283|TRHY_HUMAN Trichohyalin 36 0.085
sp|O35942|NEK2_MOUSE Serine/threonine-protein kinase Nek2 (... 36 0.085
sp|Q24318|TDP_DROME Transcription factor dp 35 0.25
sp|P35732|YKF4_YEAST Hypothetical 84.0 kDa protein in NUP12... 32 2.1
sp|O74384|ERFB_SCHPO Palmitoyltransferase erf2 (DHHC cystei... 31 2.7
sp|Q9D7G9|MISS_MOUSE MAPK-interacting and spindle-stabilizi... 30 8.0
>sp|Q07283|TRHY_HUMAN Trichohyalin
Length = 1898
Score = 36.2 bits (82), Expect = 0.085
Identities = 44/146 (30%), Positives = 64/146 (43%), Gaps = 11/146 (7%)
Frame = +2
Query: 95 AGAQHSQVLR*SYLPAAPRHRA*FRVPAPQEHGRRCASPLK--QEFVSSEQFRSQLVPAD 268
A A+ S+VL L A RA P QE RR S L+ +E + Q + + D
Sbjct: 731 ADARQSKVL----LEAPQAGRA--EAPQEQEEKRRRESELQWQEEERAHRQQQEEEQRRD 784
Query: 269 RRGQPELPEQSQQHFLVRTARVPLHDDRQALRVAEEIQQRLER---------QLGRSLRG 421
Q + E+S++ +AR PL + R+ AEE QQR +R Q GR R
Sbjct: 785 FTWQWQAEEKSERGRQRLSARPPLREQRERQLRAEERQQREQRFLPEEEEKEQRGRQRRE 844
Query: 422 TSSEIQINYEPVTQFQRSQFDELRHE 499
E+Q E +R + +L+ E
Sbjct: 845 REKELQFLEEEEQLQRRERAQQLQEE 870
>sp|O35942|NEK2_MOUSE Serine/threonine-protein kinase Nek2 (NimA-related protein kinase
2)
Length = 443
Score = 36.2 bits (82), Expect = 0.085
Identities = 24/67 (35%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
Frame = +2
Query: 206 SPLKQEFVSSEQFRSQLVPADRRGQP-ELPEQSQQHFLVRTARVPLHDDRQALRVAEEIQ 382
SPL + V+ EQ R+ R G+P +LP+ S ++ L D QALR E+I
Sbjct: 268 SPLIADMVAEEQRRNLERRGRRSGEPSKLPDSSPVLSELKLKESQLQDREQALRAREDIL 327
Query: 383 QRLERQL 403
++ ER+L
Sbjct: 328 EQKEREL 334
>sp|Q24318|TDP_DROME Transcription factor dp
Length = 445
Score = 34.7 bits (78), Expect = 0.25
Identities = 17/42 (40%), Positives = 22/42 (52%)
Frame = +3
Query: 6 DEFQTLKQSGFTLRLGTSQCTESQYKRCLELVPNTLRFYVEA 131
D+ + LK+ GF L L +CT +R VP L YVEA
Sbjct: 344 DDIEVLKRMGFLLGLDKGECTPENIERVKSWVPPNLAKYVEA 385
>sp|P35732|YKF4_YEAST Hypothetical 84.0 kDa protein in NUP120-CSE4 intergenic region
Length = 738
Score = 31.6 bits (70), Expect = 2.1
Identities = 19/77 (24%), Positives = 38/77 (49%)
Frame = +2
Query: 278 QPELPEQSQQHFLVRTARVPLHDDRQALRVAEEIQQRLERQLGRSLRGTSSEIQINYEPV 457
QP+ P+Q QQ + + P +Q + ++ QQ ++ Q + E Q++
Sbjct: 395 QPQQPQQPQQPQQQQQPQQPQQPQQQLQQQQQQQQQPVQAQ------AQAQEEQLSQNYY 448
Query: 458 TQFQRSQFDELRHEISQ 508
TQ Q+ Q+ + +H++ Q
Sbjct: 449 TQQQQQQYAQQQHQLQQ 465
>sp|O74384|ERFB_SCHPO Palmitoyltransferase erf2 (DHHC cysteine-rich domain-containing
protein erf2) (Ras protein acyltransferase)
Length = 350
Score = 31.2 bits (69), Expect = 2.7
Identities = 24/76 (31%), Positives = 30/76 (39%), Gaps = 8/76 (10%)
Frame = +3
Query: 306 STFWSEQHESPSMMTDKHYESQKRFNSASNASWGDHSGEPLQKYRLTMNPSHNFSG---- 473
S FW H SP++ Y S S D P Y LT NP+H +S
Sbjct: 105 SAFWLWHHVSPAVPITFAYLYALAVVSMFKCSTADPGILPRNAYSLTYNPAHPWSVIPED 164
Query: 474 ----VNSTSYDTKFLN 509
V ST D+ F+N
Sbjct: 165 RKVLVGSTRSDSVFVN 180
>sp|Q9D7G9|MISS_MOUSE MAPK-interacting and spindle-stabilizing protein (Mitogen-activated
protein kinase 1-interacting protein 1)
Length = 263
Score = 29.6 bits (65), Expect = 8.0
Identities = 17/62 (27%), Positives = 28/62 (45%), Gaps = 5/62 (8%)
Frame = +3
Query: 264 QIGGGSQNYRNNPSSTFWSEQHESPSMMTDKHYESQKRFNSASNASWGD-----HSGEPL 428
+I GGS + +NP ST S + + +K + ++ + +WGD H E L
Sbjct: 170 EIPGGSPSDSSNPESTLESTGQKKHLKLDNKSIKRRRSKKKSKRVTWGDIKTLTHKAESL 229
Query: 429 QK 434
K
Sbjct: 230 GK 231
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,499,991
Number of Sequences: 369166
Number of extensions: 1236197
Number of successful extensions: 3613
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 3493
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3611
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6024953540
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)