Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_O16-1
(413 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8IZF2|GP116_HUMAN Probable G-protein coupled receptor 1... 30 1.5
sp|Q8RG52|SYK_FUSNN Lysyl-tRNA synthetase (Lysine--tRNA lig... 29 4.3
sp|P37370|VRP1_YEAST Verprolin 29 4.3
sp|P34288|GAP_CAEEL GTPase-activating protein GAP (CeGAP) 28 5.6
sp|Q9WVT0|GP116_RAT Probable G-protein coupled receptor 116... 28 5.6
sp|Q17388|FER1_CAEEL Sperm vesicle fusion protein fer-1 28 7.3
sp|Q8KQR1|ISDC_STAAU Iron-regulated surface determinant pro... 28 7.3
sp|P75255|Y523_MYCPN Hypothetical lipoprotein MG348 homolog... 28 7.3
sp|Q59061|YG67_METJA Hypothetical protein MJ1667 28 7.3
sp|Q8KWX2|RPOB_EHRCH DNA-directed RNA polymerase beta chain... 28 7.3
>sp|Q8IZF2|GP116_HUMAN Probable G-protein coupled receptor 116 precursor
Length = 1346
Score = 30.4 bits (67), Expect = 1.5
Identities = 25/73 (34%), Positives = 35/73 (47%)
Frame = +3
Query: 159 LSSWLGSFARTTLLGSSTG*FSNVPLE*PFVYRGMSIFGLHFPSNFSWPKAEKRSIKFFV 338
LS W +++T LGSST FS + P R ++FG N S P+A S++
Sbjct: 1283 LSRWSSQHSKSTSLGSSTPVFS---MSSPISRRFNNLFGKTGTYNVSTPEATSSSLE--- 1336
Query: 339 VFGAGSSSDGSML 377
SSS S+L
Sbjct: 1337 ----NSSSASSLL 1345
>sp|Q8RG52|SYK_FUSNN Lysyl-tRNA synthetase (Lysine--tRNA ligase) (LysRS)
Length = 493
Score = 28.9 bits (63), Expect = 4.3
Identities = 23/65 (35%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
Frame = -2
Query: 298 EKFEGKCKPKIDIPRYTNGHSNGTFENQPVELPSNVVRAND-PSHDDRPEQGITK-NLVN 125
E FE KC+ K+ P + GH PVE+ R D P+ DR E I K N
Sbjct: 361 EFFEQKCEEKVIQPTFVYGH--------PVEISPLAKRNEDNPNFTDRFELFINKREYAN 412
Query: 124 RFREM 110
F E+
Sbjct: 413 AFTEL 417
>sp|P37370|VRP1_YEAST Verprolin
Length = 817
Score = 28.9 bits (63), Expect = 4.3
Identities = 20/69 (28%), Positives = 31/69 (44%), Gaps = 17/69 (24%)
Frame = -2
Query: 388 TQTISMEPSDELPAP------------NTTKNLMERFSALGQEKFEGKC-----KPKIDI 260
T++IS P+ P P + +KNL +R + G + K +P +D+
Sbjct: 693 TESISKNPTKSPPPPPSPSTMDTGTSNSPSKNLKQRLFSTGGSTLQHKHNTHTNQPDVDV 752
Query: 259 PRYTNGHSN 233
RYT G SN
Sbjct: 753 GRYTIGGSN 761
>sp|P34288|GAP_CAEEL GTPase-activating protein GAP (CeGAP)
Length = 1317
Score = 28.5 bits (62), Expect = 5.6
Identities = 18/54 (33%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = -2
Query: 250 TNGHSNGTFENQPVELPSNVVRANDPSHDDRPE-QGITKNLVNRFREMEGSSNN 92
+NG T E+Q V S + D HDD E Q I++ + R R S N
Sbjct: 18 SNGGGTNTEEDQTVSATSKEDPSEDTGHDDPEETQEISETPIRRSRNRNASLRN 71
>sp|Q9WVT0|GP116_RAT Probable G-protein coupled receptor 116 precursor (G-protein coupled
hepta-helical receptor Ig-hepta)
Length = 1349
Score = 28.5 bits (62), Expect = 5.6
Identities = 19/57 (33%), Positives = 28/57 (49%)
Frame = +3
Query: 159 LSSWLGSFARTTLLGSSTG*FSNVPLE*PFVYRGMSIFGLHFPSNFSWPKAEKRSIK 329
LS W +++T LGSST FS + P R ++FG N S P+ S++
Sbjct: 1286 LSRWSSQHSKSTSLGSSTPVFS---MSSPISRRFNNLFGKTGTYNVSTPETTSSSVE 1339
>sp|Q17388|FER1_CAEEL Sperm vesicle fusion protein fer-1
Length = 2034
Score = 28.1 bits (61), Expect = 7.3
Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = -3
Query: 258 LDIQTVIPMEH-LKINLLNSQAMLFEQTIPAMTIDQNKALPRTW 130
+D+ IP+E LKI ++ + +L +Q I + TID L W
Sbjct: 1436 VDMTVTIPLEKDLKITVMGKRRILSDQEIGSTTIDLENRLLTKW 1479
>sp|Q8KQR1|ISDC_STAAU Iron-regulated surface determinant protein d precursor
sp|Q6GHV5|ISDC_STAAR Iron-regulated surface determinant protein d precursor
Length = 227
Score = 28.1 bits (61), Expect = 7.3
Identities = 24/77 (31%), Positives = 34/77 (44%), Gaps = 9/77 (11%)
Frame = -2
Query: 361 DELPAPNTTKNLMERFSALGQEKFEGKCKPKIDI---------PRYTNGHSNGTFENQPV 209
+ + + NT K+ ER S K GK KID+ P + H N T++
Sbjct: 88 ERIISKNTAKD--ERTSEFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNG- 144
Query: 208 ELPSNVVRANDPSHDDR 158
PS+V AN P DD+
Sbjct: 145 --PSDVAGANAPGKDDK 159
>sp|P75255|Y523_MYCPN Hypothetical lipoprotein MG348 homolog precursor (G12_orf305)
Length = 305
Score = 28.1 bits (61), Expect = 7.3
Identities = 22/80 (27%), Positives = 40/80 (50%)
Frame = +3
Query: 153 SGLSSWLGSFARTTLLGSSTG*FSNVPLE*PFVYRGMSIFGLHFPSNFSWPKAEKRSIKF 332
S +S SF+ LLGS S ++ P V+R +S + ++S E ++++
Sbjct: 6 SSISKKKVSFSTLLLLGSGIVLSSCSNIDKPNVFRTLSQQSVENKVDYSKLPKENKTVR- 64
Query: 333 FVVFGAGSSSDGSMLIVCVT 392
+VFG +DG+ ++V T
Sbjct: 65 NLVFGTAEYNDGNYVLVVTT 84
>sp|Q59061|YG67_METJA Hypothetical protein MJ1667
Length = 418
Score = 28.1 bits (61), Expect = 7.3
Identities = 22/72 (30%), Positives = 31/72 (43%), Gaps = 10/72 (13%)
Frame = -2
Query: 277 KPKIDIPRYTNGHSNGTF------ENQPVELPSNVVRANDPSHDDRPEQGITKNLVNRFR 116
K K D+P G + GTF E++ + + ++ N DD + I KNL N F
Sbjct: 241 KKKFDVPINMEGMTKGTFSINIKIEDEFFKNINKRLKTNYNPKDDEKKFDILKNLCNNFS 300
Query: 115 ----EMEGSSNN 92
E E NN
Sbjct: 301 KTVVEFELKKNN 312
>sp|Q8KWX2|RPOB_EHRCH DNA-directed RNA polymerase beta chain (RNAP beta subunit)
(Transcriptase beta chain) (RNA polymerase beta subunit)
Length = 1378
Score = 28.1 bits (61), Expect = 7.3
Identities = 19/73 (26%), Positives = 33/73 (45%), Gaps = 4/73 (5%)
Frame = -2
Query: 310 ALGQEKFEGKCKPKIDIPRYTNGHSNGTFENQPVELPSNVVRANDPSHD----DRPEQGI 143
A QEK E ++ ++ + N +P+ P + V+AND D D+ E +
Sbjct: 757 AFDQEKNEYLGVDIYNLRKFQRSNHNTCINQRPLVKPGDYVKANDVIADGSAIDQGELAL 816
Query: 142 TKNLVNRFREMEG 104
KN++ F +G
Sbjct: 817 GKNVLVAFMSWQG 829
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,396,022
Number of Sequences: 369166
Number of extensions: 1069721
Number of successful extensions: 2695
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2628
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2694
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 1902516170
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)