Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_O14
(374 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9XES1|ECA4_ARATH Calcium-transporting ATPase 4, endopla... 30 1.7
sp|Q59043|YG49_METJA Hypothetical UPF0064 protein MJ1649 29 2.9
sp|Q4J6G3|THII_SULAC Probable thiamine biosynthesis protein... 28 5.0
sp|P59420|SYA_BUCBP Alanyl-tRNA synthetase (Alanine--tRNA l... 28 5.0
sp|O94833|BPAEA_HUMAN Bullous pemphigoid antigen 1, isoform... 28 5.0
sp|Q8XPA2|GLGB1_CLOPE 1,4-alpha-glucan branching enzyme 1 (... 28 6.5
sp|P45360|Y2874_CLOAB Putative UDP-N-acetylglucosamine 2-ep... 28 8.5
sp|P53255|YG2M_YEAST Hypothetical 58.2 kDa protein in DBF2-... 28 8.5
>sp|Q9XES1|ECA4_ARATH Calcium-transporting ATPase 4, endoplasmic reticulum-type
Length = 1061
Score = 30.0 bits (66), Expect = 1.7
Identities = 18/41 (43%), Positives = 27/41 (65%), Gaps = 1/41 (2%)
Frame = +2
Query: 101 KSDDKPPF-KSLSEREEKFFIELQEKLYREELIRCHQFIYG 220
KSD P + K +SE EEKF + ++ L +E+++ HQ IYG
Sbjct: 20 KSDTFPAWGKDVSECEEKFGVSREKGLSTDEVLKRHQ-IYG 59
>sp|Q59043|YG49_METJA Hypothetical UPF0064 protein MJ1649
Length = 387
Score = 29.3 bits (64), Expect = 2.9
Identities = 17/57 (29%), Positives = 30/57 (52%), Gaps = 3/57 (5%)
Frame = +2
Query: 173 KLYREELIR---CHQFIYGSQYNPMNIEPFLFDRERLSGAYTEQDRLLRKQYLEDIV 334
K+YRE ++ +++IY + +FD+ GA +L+ K+YL+DIV
Sbjct: 87 KIYRENILNYKNTYRWIYAEGDE---LPTIIFDKYNELGAMQLMSKLIEKEYLKDIV 140
>sp|Q4J6G3|THII_SULAC Probable thiamine biosynthesis protein thiI
Length = 369
Score = 28.5 bits (62), Expect = 5.0
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +2
Query: 83 YCLIMGKSDDKPPFKSLSEREEKFFIELQEKLYREELIR 199
YC+I KS K + E E K I +E + + EL+R
Sbjct: 330 YCVISNKSIVKSQLDKVLEEERKIEINYEELIKKAELVR 368
>sp|P59420|SYA_BUCBP Alanyl-tRNA synthetase (Alanine--tRNA ligase) (AlaRS)
Length = 880
Score = 28.5 bits (62), Expect = 5.0
Identities = 21/69 (30%), Positives = 33/69 (47%)
Frame = +3
Query: 117 HRLNL*VSARKSFLLSYKKNYIEKN*LDVINLYMGHNTIL*ILNHFFLIERGFQEPTLNK 296
H +++ + K +L +K+N I KN L+ N Y + + I HF G+Q NK
Sbjct: 417 HNISINIMEFKRYLNQHKQNSINKNFLNTRNAYYIEDNNINIKTHFV----GYQ---FNK 469
Query: 297 IDF*ENNIL 323
NNI+
Sbjct: 470 TQSIINNII 478
>sp|O94833|BPAEA_HUMAN Bullous pemphigoid antigen 1, isoforms 6/9/10 (Trabeculin-beta)
(Bullous pemphigoid antigen) (BPA) (Hemidesmosomal plaque
protein) (Dystonia musculorum protein) (Dystonin)
Length = 5171
Score = 28.5 bits (62), Expect = 5.0
Identities = 22/110 (20%), Positives = 56/110 (50%), Gaps = 21/110 (19%)
Frame = +2
Query: 92 IMGKSDDKPPFKSLSEREEKFFIELQEKLY-REELIR---CHQFIYG------------- 220
++ +DK +S + + ++IE+QEK + R EL++ C+ I+G
Sbjct: 3158 VLSSREDKDMVQSKLDFSQVWYIEIQEKSHSRSELLQQALCNAKIFGEDEVELMNWLNEV 3217
Query: 221 -SQYNPMNIEPFLFD---RERLSGAYTEQDRLLRKQYLEDIVMSSIEPVK 358
+ + ++++ + + +++ ++D LLRKQ ++ +++ +E +K
Sbjct: 3218 HDKLSKLSVQDYSTEGLWKQQSELRVLQEDILLRKQNVDQALLNGLELLK 3267
>sp|Q8XPA2|GLGB1_CLOPE 1,4-alpha-glucan branching enzyme 1 (Glycogen branching enzyme 1)
(BE 1) (1,4-alpha-D-glucan:1,4-alpha-D-glucan
6-glucosyl-transferase 1)
Length = 674
Score = 28.1 bits (61), Expect = 6.5
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +2
Query: 107 DDKPPFKSLSEREEKFFIELQEKLYREELIRCHQFIYGSQYNPMN 241
DDK K + R+EK+ +L+ K++ E+ + + F G YN N
Sbjct: 8 DDKLEIKPVRLRKEKYKTQLKNKIFNEDDL--YLFHEGRNYNAYN 50
>sp|P45360|Y2874_CLOAB Putative UDP-N-acetylglucosamine 2-epimerase
(UDP-GlcNAc-2-epimerase)
Length = 385
Score = 27.7 bits (60), Expect = 8.5
Identities = 14/57 (24%), Positives = 31/57 (54%)
Frame = +3
Query: 159 LSYKKNYIEKN*LDVINLYMGHNTIL*ILNHFFLIERGFQEPTLNKIDF*ENNILKI 329
L KKN + + ++ N+++ NT++ +NH + F+ LNK+D+ ++ +
Sbjct: 153 LGSKKNLLREA-VNEKNIFITGNTVVDAMNHTVEKDYVFENDELNKLDYKNKKVIMV 208
>sp|P53255|YG2M_YEAST Hypothetical 58.2 kDa protein in DBF2-VAS1 intergenic region
Length = 507
Score = 27.7 bits (60), Expect = 8.5
Identities = 22/68 (32%), Positives = 36/68 (52%), Gaps = 8/68 (11%)
Frame = +2
Query: 98 GKSDDKPPFK-------SLSEREEKFF-IELQEKLYREELIRCHQFIYGSQYNPMNIEPF 253
GKS+D+ S+SER + E+ ++L ++ R H F + + N +EPF
Sbjct: 116 GKSEDQVDILLTKEWGLSISERCGRLSGSEVVDELAKKLQARYH-FAFSDEINFYELEPF 174
Query: 254 LFDRERLS 277
++RERLS
Sbjct: 175 QWERERLS 182
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,409,903
Number of Sequences: 369166
Number of extensions: 625560
Number of successful extensions: 1487
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1466
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1486
length of database: 68,354,980
effective HSP length: 91
effective length of database: 51,544,095
effective search space used: 1700955135
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)