Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_M23
(846 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 122 2e-27
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 121 2e-27
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 121 2e-27
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 121 3e-27
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 120 4e-27
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 118 2e-26
sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1 118 2e-26
sp|P41892|CDC7_SCHPO Cell division control protein 7 115 1e-25
sp|P27636|CDC15_YEAST Cell division control protein 15 114 3e-25
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 114 3e-25
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 122 bits (305), Expect = 2e-27
Identities = 79/220 (35%), Positives = 116/220 (52%), Gaps = 6/220 (2%)
Frame = +3
Query: 195 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDE---ELKKLMVEFSIYEKLGDHPRILK 365
LG G FG VY C + + K+ + D E E+ L E + + L H RI++
Sbjct: 368 LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNL-QHERIVQ 426
Query: 366 CNGFFR--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNIL 539
G R + K I ++YM GS++ L GA E ++++QILE + +LHS I+
Sbjct: 427 YYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIV 486
Query: 540 HRDLKGPKILLDNNLDIKLCGFMSAKMFPD-ASSETFRNHVAESNHGTANWTAPEVINGE 716
HRD+KG IL D+ ++KL F ++K S T V GT W +PEVI+GE
Sbjct: 487 HRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVT----GTPYWMSPEVISGE 542
Query: 717 GYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQ 836
GYG AD+WS G +VE++T K P + AI+++ TQ
Sbjct: 543 GYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQ 582
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 121 bits (304), Expect = 2e-27
Identities = 78/226 (34%), Positives = 124/226 (54%), Gaps = 7/226 (3%)
Frame = +3
Query: 183 KHEELGHGPFGSVYRCSKFGSKDMVVLKKY---KNPNDKDE---ELKKLMVEFSIYEKLG 344
K + +G G FG+VY S +++ +K+ N K++ +++L E + + L
Sbjct: 70 KGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLLKNLS 129
Query: 345 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 524
HP I++ G R + I L+++ GS+ S L K GA E + ++ Q+L L++LH
Sbjct: 130 -HPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLGLEYLH 188
Query: 525 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 704
+ I+HRD+KG IL+DN IKL F ++K + ++ + A+S GT W APEV
Sbjct: 189 NHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATIS----GAKSMKGTPYWMAPEV 244
Query: 705 INGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQK 839
I G+ SADIWS G ++E++TGKAP AI+ +GT K
Sbjct: 245 ILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTK 290
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 121 bits (304), Expect = 2e-27
Identities = 78/226 (34%), Positives = 123/226 (54%), Gaps = 7/226 (3%)
Frame = +3
Query: 183 KHEELGHGPFGSVYRCSKFGSKDMVVLKKYK-NPNDKDEE-----LKKLMVEFSIYEKLG 344
K E +G G FG VY S +++ +K+ P+ +E +++L E + + L
Sbjct: 70 KGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLKNLS 129
Query: 345 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 524
HP I++ G R + I ++++ GS+ S L K G+ EP II ++KQ+L L++LH
Sbjct: 130 -HPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLH 188
Query: 525 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 704
+ I+HRD+KG IL+DN I+L F ++K + ++ + A+S GT W APEV
Sbjct: 189 NNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATV----NGAKSMKGTPYWMAPEV 244
Query: 705 INGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQK 839
I G+ SADIWS G ++E+ TGK P F A+ +G K
Sbjct: 245 ILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTK 290
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 121 bits (303), Expect = 3e-27
Identities = 77/219 (35%), Positives = 115/219 (52%), Gaps = 5/219 (2%)
Frame = +3
Query: 195 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDE---ELKKLMVEFSIYEKLGDHPRILK 365
LG G FG VY C + + K+ + D E E+ L E + + L H RI++
Sbjct: 368 LGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNL-QHERIVQ 426
Query: 366 CNGFFR--SVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNIL 539
G R + K I ++YM GS++ L GA E ++++QILE + +LHS I+
Sbjct: 427 YYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIV 486
Query: 540 HRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEG 719
HRD+KG IL D+ ++KL F ++K + S GT W +PEVI+GEG
Sbjct: 487 HRDIKGANILRDSAGNVKLGDFGASKRL---QTICMSGTGIRSVTGTPYWMSPEVISGEG 543
Query: 720 YGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQ 836
YG AD+WS G +VE++T K P + AI+++ TQ
Sbjct: 544 YGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQ 582
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 120 bits (302), Expect = 4e-27
Identities = 76/226 (33%), Positives = 121/226 (53%), Gaps = 7/226 (3%)
Frame = +3
Query: 183 KHEELGHGPFGSVYRCSKFGSKDMVVLKK------YKNPNDKDEELKKLMVEFSIYEKLG 344
K + +G G FG+VY S +++ +K+ + + +++L E + + L
Sbjct: 71 KGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKNLS 130
Query: 345 DHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLH 524
HP I++ G R I L+++ GS+ S L K G E + +++Q+L L++LH
Sbjct: 131 -HPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLLGLEYLH 189
Query: 525 SQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEV 704
+ I+HRD+KG IL+DN IKL F ++K + ++ T A+S GT W APEV
Sbjct: 190 NHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMT----GAKSMKGTPYWMAPEV 245
Query: 705 INGEGYGSSADIWSFGAVMVELITGKAPLF-FLASFQAIYRLGTQK 839
I G+ SADIWS G ++E++TGKAP AI+ +GT K
Sbjct: 246 ILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTK 291
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 118 bits (296), Expect = 2e-26
Identities = 76/219 (34%), Positives = 114/219 (52%), Gaps = 5/219 (2%)
Frame = +3
Query: 195 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDE---ELKKLMVEFSIYEKLGDHPRILK 365
LG G FG VY C + + +K+ + D E E+ L E + + H RI++
Sbjct: 361 LGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNFL-HERIVQ 419
Query: 366 CNGFFRSV--KASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNIL 539
G R K I ++YM GS++ L GA E ++++QILE + +LHS IL
Sbjct: 420 YYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYLHSNMIL 479
Query: 540 HRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEG 719
HRD+KG IL D+ ++KL F ++K + +S GT W +PEVI+G+G
Sbjct: 480 HRDIKGANILRDSTGNVKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPEVISGQG 536
Query: 720 YGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQ 836
YG ADIWS +VE++T K P + AI+++ TQ
Sbjct: 537 YGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQ 575
>sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1
Length = 1116
Score = 118 bits (295), Expect = 2e-26
Identities = 73/232 (31%), Positives = 128/232 (55%), Gaps = 10/232 (4%)
Frame = +3
Query: 177 YIKHEELGHGPFGSVYRCSKFGSKDMVVLKKYKNP---NDKDEELKKLMV-----EFSIY 332
++K E +G+G +G V+ + +++ +K+ + P N + ++L+K +V E S+
Sbjct: 825 WMKGELIGNGTYGKVFLAMNINTGELIAVKQVEIPQTINGRHDQLRKDIVDSINAEISMI 884
Query: 333 EKLGDHPRILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEAL 512
L DH I++ GF ++ I L+Y++ GS+ L G +E + S+Q+L L
Sbjct: 885 ADL-DHLNIVQYLGFEKTETDISIFLEYVSGGSIGRCLRNYGPFEEQLVRFVSRQVLYGL 943
Query: 513 DFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWT 692
+LHS+ I+HRDLK +L+D + K+ F +K S + N S G+ W
Sbjct: 944 SYLHSKGIIHRDLKADNLLIDFDGVCKISDFGISK----HSDNVYDNDANLSMQGSIFWM 999
Query: 693 APEVING--EGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKK 842
APEVI+ +GY + D+WS G V++E++ G+ P + QA+++LGT+KK
Sbjct: 1000 APEVIHNDHQGYSAKVDVWSLGCVVLEMLAGRRPWSTDEAIQAMFKLGTEKK 1051
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 115 bits (289), Expect = 1e-25
Identities = 72/211 (34%), Positives = 110/211 (52%)
Frame = +3
Query: 195 LGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNG 374
LG G FG+VYR + + V +KK K +L + +E + + L DHP I+K G
Sbjct: 15 LGKGAFGAVYRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLLKNL-DHPNIVKYRG 73
Query: 375 FFRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLK 554
+++ + I L+Y NGSLRS G E + ++ Q+L+ L +LH+Q ++HRD+K
Sbjct: 74 SYQTNDSLCIILEYCENGSLRSICKNFGKIPENLVALYTFQVLQGLLYLHNQGVIHRDIK 133
Query: 555 GPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSA 734
G IL + IKL F A +H S G+ W APEVI G +++
Sbjct: 134 GANILTTKDGTIKLADFGVA-----TKINALEDH---SVVGSPYWMAPEVIELVGATTAS 185
Query: 735 DIWSFGAVMVELITGKAPLFFLASFQAIYRL 827
DIWS G ++EL+ G P + L A++R+
Sbjct: 186 DIWSVGCTVIELLDGNPPYYDLDPTSALFRM 216
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 114 bits (286), Expect = 3e-25
Identities = 72/216 (33%), Positives = 106/216 (49%), Gaps = 1/216 (0%)
Frame = +3
Query: 177 YIKHEELGHGPFGSVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPR 356
Y + +G G +G VY+ + +V +K+ ND EEL +M E S+ + L +H
Sbjct: 25 YHLKQVIGRGSYGVVYKAINKHTDQVVAIKEVVYEND--EELNDIMAEISLLKNL-NHNN 81
Query: 357 ILKCNGFFRSVKASYIKLDYMANGSLRSWLHKNGAQ-KEPSIIRFSKQILEALDFLHSQN 533
I+K +GF R YI L+Y ANGSLR + ++ E + Q L L +LH +
Sbjct: 82 IVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEG 141
Query: 534 ILHRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVING 713
++HRD+K ILL + +KL F T N A + GT NW APE++
Sbjct: 142 VIHRDIKAANILLSADNTVKLADF---------GVSTIVNSSALTLAGTLNWMAPEILGN 192
Query: 714 EGYGSSADIWSFGAVMVELITGKAPLFFLASFQAIY 821
G + +DIWS GA +VE++T P L Y
Sbjct: 193 RGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYY 228
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 114 bits (286), Expect = 3e-25
Identities = 76/219 (34%), Positives = 115/219 (52%), Gaps = 5/219 (2%)
Frame = +3
Query: 195 LGHGPFGSVYRCSKFGS-KDMVVLKKYKNPNDKD--EELKKLMVEFSIYEKLGDHPRILK 365
LG G FG VY C + +++ V + NP + +E+ L E + + L H RI++
Sbjct: 362 LGQGAFGRVYLCYDVDTGRELAVKQVQFNPESPETSKEVNALECEIQLLKNLL-HERIVQ 420
Query: 366 CNGFFRSV--KASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNIL 539
G R K I ++ GS++ L GA E ++++QILE + +LHS I+
Sbjct: 421 YYGCLRDPQEKTLSIFMELSPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIV 480
Query: 540 HRDLKGPKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEG 719
HRD+KG IL D+ +IKL F ++K + +S GT W +PEVI+GEG
Sbjct: 481 HRDIKGANILRDSTGNIKLGDFGASKRL---QTICLSGTGMKSVTGTPYWMSPEVISGEG 537
Query: 720 YGSSADIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQ 836
YG ADIWS +VE++T K P + AI+++ TQ
Sbjct: 538 YGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQ 576
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,278,430
Number of Sequences: 369166
Number of extensions: 2121911
Number of successful extensions: 9658
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7300
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7779
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8293644780
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)