Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_M15
(789 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P06872|TRY2_CANFA Anionic trypsin precursor 131 2e-30
sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-l... 130 3e-30
sp|P35033|TRY3_SALSA Trypsin III precursor 127 3e-29
sp|P15949|KLK9_MOUSE Glandular kallikrein K9 precursor (Tis... 127 5e-29
sp|Q29463|TRY2_BOVIN Anionic trypsin precursor 125 2e-28
sp|Q03238|GRAM_RAT Granzyme M precursor (Met-ase) (Natural ... 124 4e-28
sp|P80646|CTRB_GADMO Chymotrypsin B [Contains: Chymotrypsin... 124 4e-28
sp|P00762|TRY1_RAT Anionic trypsin I precursor (Pretrypsino... 123 5e-28
sp|P00760|TRY1_BOVIN Cationic trypsin precursor (Beta-tryps... 123 5e-28
sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma p... 123 7e-28
>sp|P06872|TRY2_CANFA Anionic trypsin precursor
Length = 247
Score = 131 bits (330), Expect = 2e-30
Identities = 84/248 (33%), Positives = 126/248 (50%), Gaps = 1/248 (0%)
Frame = +3
Query: 6 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 185
D D KI+ G + P VS+ G H CGG+L++ V++AAHC
Sbjct: 19 DDDDKIVGGYTCEENSVPYQVSLNA-GYHFCGGSLISDQWVVSAAHCYKS---------- 67
Query: 186 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNG-FISNYD 362
+++V +GEYN++ LEG E+ K I HP+Y +++ ND+ +IK + + N
Sbjct: 68 --RIQVRLGEYNIDVLEGNEQFINSAKVIRHPNYN---SWILDNDIMLIKLSSPAVLNAR 122
Query: 363 VEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKTYRNV 542
V I + + + PG +C++ GWG+ L G + E LQ D I++ +C +Y
Sbjct: 123 VATISLPR----ACAAPGTQCLISGWGNTLSSGTNYPELLQCLDAPILTQAQCEASYPG- 177
Query: 543 QFVPSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTVYTR 722
Q + ICAG K +C+GDSGGP+ C GIVS+ + C Q NKP VYT+
Sbjct: 178 QITENMICAGFLEGGKDSCQGDSGGPVVCNGEL-----QGIVSWGYG-CAQKNKPGVYTK 231
Query: 723 VSEVAKWI 746
V WI
Sbjct: 232 VCNFVDWI 239
>sp|Q9UKR3|KLK13_HUMAN Kallikrein 13 precursor (Kallikrein-like protein 4) (KLK-L4)
Length = 277
Score = 130 bits (328), Expect = 3e-30
Identities = 76/234 (32%), Positives = 123/234 (52%), Gaps = 1/234 (0%)
Frame = +3
Query: 48 HEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLEYHQVKVVIGEYNVN 227
H P ++ G +CGG L++ VLTAAHC+ E +KV +G++ +
Sbjct: 45 HSQPWQAALLVQGRLLCGGVLVHPKWVLTAAHCLKE------------GLKVYLGKHALG 92
Query: 228 QLEGTEKIYQVTKTIMHPSYKREKAYVD-GNDVAVIKFNGFISNYDVEYIKYVKLDTNSI 404
++E E++ +V +I HP Y+R +++ +D+ +++ + YI+ + L N+
Sbjct: 93 RVEAGEQVREVVHSIPHPEYRRSPTHLNHDHDIMLLELQSPVQL--TGYIQTLPLSHNNR 150
Query: 405 SKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKTYRNVQFVPSNICAGSGRL 584
PG C V GWG+ + + LQ A++ + S EEC + Y + + +CAG+
Sbjct: 151 LTPGTTCRVSGWGTTTSPQVNYPKTLQCANIQLRSDEECRQVYPG-KITDNMLCAGTKEG 209
Query: 585 SKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTVYTRVSEVAKWI 746
K +C+GDSGGPL C + GIVS+ PCGQ ++P VYTRVS WI
Sbjct: 210 GKDSCEGDSGGPLVCNRTL-----YGIVSWGDFPCGQPDRPGVYTRVSRYVLWI 258
>sp|P35033|TRY3_SALSA Trypsin III precursor
Length = 238
Score = 127 bits (319), Expect = 3e-29
Identities = 85/248 (34%), Positives = 125/248 (50%)
Frame = +3
Query: 6 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 185
D D KI+ G + S+ Q G H CGG+L++ V++AAHC
Sbjct: 11 DEDDKIVGGYECRKNSASYQASL-QSGYHFCGGSLISSTWVVSAAHCYKS---------- 59
Query: 186 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFISNYDV 365
+++V +GE+N+ EGTE+ K IMHPSY ND+ +IK + S
Sbjct: 60 --RIQVRLGEHNIAVNEGTEQFIDSVKVIMHPSYNSRNL---DNDIMLIKLSKPASLN-- 112
Query: 366 EYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKTYRNVQ 545
Y+ V L +S + G RC+V GWG++ + + L+ DL I+S+ C+ Y Q
Sbjct: 113 SYVSTVAL-PSSCASSGTRCLVSGWGNLSGSSSNYPDTLRCLDLPILSSSSCNSAYPG-Q 170
Query: 546 FVPSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTVYTRV 725
+ CAG K +C+GDSGGP+ C Q+Q G+VS+ + C Q NKP VYT+V
Sbjct: 171 ITSNMFCAGFMEGGKDSCQGDSGGPVVCNG----QLQ-GVVSWGYG-CAQRNKPGVYTKV 224
Query: 726 SEVAKWIA 749
WI+
Sbjct: 225 CNYRSWIS 232
>sp|P15949|KLK9_MOUSE Glandular kallikrein K9 precursor (Tissue kallikrein 9) (mGK-9)
(Epidermal growth factor-binding protein type C) (EGF-BP
C)
Length = 261
Score = 127 bits (318), Expect = 5e-29
Identities = 80/251 (31%), Positives = 124/251 (49%), Gaps = 8/251 (3%)
Frame = +3
Query: 18 KIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLEYHQV 197
+I+ G + + P V++ + E++CGG LL+ N VLTAAHC Y +
Sbjct: 24 RIVGGFKCEKNSQPWHVAVYRYNEYICGGVLLDANWVLTAAHCY------------YEEN 71
Query: 198 KVVIGEYNVNQLEGTEKIYQVTKTIMHPSYK--------REKAYVDGNDVAVIKFNGFIS 353
KV +G+ N+ + E + + V+K+ +HP Y R Y ND+ +++ +
Sbjct: 72 KVSLGKNNLYEEEPSAQHRLVSKSFLHPGYNRSLHRNHIRHPEYDYSNDLMLLRLSKPAD 131
Query: 354 NYDVEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKTY 533
DV +K + L T K G C+ GWGS P A+ LQ +L ++ E+C K +
Sbjct: 132 ITDV--VKPIALPTEE-PKLGSTCLASGWGSTTPFKFQNAKDLQCVNLKLLPNEDCGKAH 188
Query: 534 RNVQFVPSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTV 713
+ +CAG K CKGDSGGPL C + + GI S+ +PCG+ KP V
Sbjct: 189 IE-KVTDVMLCAGETDGGKDTCKGDSGGPLIC-----DGVLQGITSWGFTPCGEPKKPGV 242
Query: 714 YTRVSEVAKWI 746
YT++ + WI
Sbjct: 243 YTKLIKFTSWI 253
>sp|Q29463|TRY2_BOVIN Anionic trypsin precursor
Length = 247
Score = 125 bits (313), Expect = 2e-28
Identities = 85/248 (34%), Positives = 127/248 (51%), Gaps = 1/248 (0%)
Frame = +3
Query: 6 DADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLE 185
D D KI+ G + P VS+ G H CGG+L+N V++AAHC +
Sbjct: 19 DDDDKIVGGYTCAENSVPYQVSLNA-GYHFCGGSLINDQWVVSAAHCY-----------Q 66
Query: 186 YHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFN-GFISNYD 362
YH ++V +GEYN++ LEG E+ +K I HP Y ++ ND+ +IK + + N
Sbjct: 67 YH-IQVRLGEYNIDVLEGGEQFIDASKIIRHPKY---SSWTLDNDILLIKLSTPAVINAR 122
Query: 363 VEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKTYRNV 542
V + L ++ + G C++ GWG+ L G + + LQ ++S +C +Y
Sbjct: 123 VSTL----LLPSACASAGTECLISGWGNTLSSGVNYPDLLQCLVAPLLSHADCEASYPG- 177
Query: 543 QFVPSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTVYTR 722
Q + ICAG K +C+GDSGGP+ C Q+Q GIVS+ + C Q KP VYT+
Sbjct: 178 QITNNMICAGFLEGGKDSCQGDSGGPVACNG----QLQ-GIVSWGYG-CAQKGKPGVYTK 231
Query: 723 VSEVAKWI 746
V WI
Sbjct: 232 VCNYVDWI 239
>sp|Q03238|GRAM_RAT Granzyme M precursor (Met-ase) (Natural killer cell granular
protease) (RNK-Met-1)
Length = 258
Score = 124 bits (310), Expect = 4e-28
Identities = 81/246 (32%), Positives = 124/246 (50%), Gaps = 3/246 (1%)
Frame = +3
Query: 18 KIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLEYHQV 197
+II G V H P +VS+ H+CGG L++ VLTAAHC+ E Q+
Sbjct: 20 QIIGGREAVPHSRPYMVSLQNTKSHMCGGVLVHQKWVLTAAHCLSE---------PLQQL 70
Query: 198 KVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFISNYDVEYIK 377
K+V G ++++ + + + + I HP Y + ND+A++K +G + + +K
Sbjct: 71 KLVFGLHSLHDPQDPGLTFYIKQAIKHPGYNLKYE----NDLALLKLDGRVK--PSKNVK 124
Query: 378 YVKLDTNSISKP--GDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKT-YRNVQF 548
+ L KP G RC GWG G+ A+ LQ+ DL ++ T C+ + + N
Sbjct: 125 PLALPRKPRDKPAEGSRCSTAGWGITHQRGQ-LAKSLQELDLRLLDTRMCNNSRFWNGVL 183
Query: 549 VPSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTVYTRVS 728
S +C +G ++ CKGDSGGPL C G+ GI+S+S C I KPTV T V+
Sbjct: 184 TDSMLCLKAGAKGQAPCKGDSGGPLVC----GKGKVDGILSFSSKNCTDIFKPTVATAVA 239
Query: 729 EVAKWI 746
+ WI
Sbjct: 240 PYSSWI 245
>sp|P80646|CTRB_GADMO Chymotrypsin B [Contains: Chymotrypsin B chain A; Chymotrypsin B
chain B]
Length = 245
Score = 124 bits (310), Expect = 4e-28
Identities = 82/245 (33%), Positives = 131/245 (53%), Gaps = 2/245 (0%)
Frame = +3
Query: 18 KIINGSRTVLHEYPEIVSITQ-DGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLEYHQ 194
+I+NG V H +P VS+ Q +G H CGG+L+N N V+TAAHC YH
Sbjct: 15 RIVNGEEAVPHSWPWQVSLQQSNGFHFCGGSLINENWVVTAAHC---------NVRTYH- 64
Query: 195 VKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKF-NGFISNYDVEY 371
+V++GE++ + +++ Q+ K M ++ + + ND+++IK + + +V
Sbjct: 65 -RVIVGEHD----KASDENIQILKPSMVFTHPKWDSRTINNDISLIKLASPAVLGTNVSP 119
Query: 372 IKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKTYRNVQFV 551
+ +++ + PG +CV GWG + T LQ+A L ++S EECS+T+ N
Sbjct: 120 VCLG--ESSDVFAPGMKCVTSGWGLTRYNAPGTPNKLQQAALPLMSNEECSQTWGNNMIS 177
Query: 552 PSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTVYTRVSE 731
ICAG+ ++C GDSGGPL C+ VGIVS+ S C + P VY RV+E
Sbjct: 178 DVMICAGAA--GATSCMGDSGGPLVCQKD-NVWTLVGIVSWGSSRC-SVTTPAVYARVTE 233
Query: 732 VAKWI 746
+ W+
Sbjct: 234 LRGWV 238
>sp|P00762|TRY1_RAT Anionic trypsin I precursor (Pretrypsinogen I)
Length = 246
Score = 123 bits (309), Expect = 5e-28
Identities = 80/249 (32%), Positives = 125/249 (50%), Gaps = 1/249 (0%)
Frame = +3
Query: 3 VDADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWL 182
++ D KI+ G H P VS+ G H CGG+L+N V++AAHC
Sbjct: 18 LEDDDKIVGGYTCPEHSVPYQVSLNS-GYHFCGGSLINDQWVVSAAHCYKS--------- 67
Query: 183 EYHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFIS-NY 359
+++V +GE+N+N LEG E+ K I HP+Y ++ ND+ +IK + + N
Sbjct: 68 ---RIQVRLGEHNINVLEGDEQFINAAKIIKHPNY---SSWTLNNDIMLIKLSSPVKLNA 121
Query: 360 DVEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKTYRN 539
V + ++ + G +C++ GWG+ L +G + + LQ D ++S +C Y
Sbjct: 122 RVAPVAL----PSACAPAGTQCLISGWGNTLSNGVNNPDLLQCVDAPVLSQADCEAAYPG 177
Query: 540 VQFVPSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTVYT 719
+ S IC G K +C+GDSGGP+ C Q+Q GIVS+ + C + P VYT
Sbjct: 178 -EITSSMICVGFLEGGKDSCQGDSGGPVVCNG----QLQ-GIVSWGYG-CALPDNPGVYT 230
Query: 720 RVSEVAKWI 746
+V WI
Sbjct: 231 KVCNFVGWI 239
>sp|P00760|TRY1_BOVIN Cationic trypsin precursor (Beta-trypsin) [Contains: Alpha-trypsin
chain 1; Alpha-trypsin chain 2]
Length = 243
Score = 123 bits (309), Expect = 5e-28
Identities = 83/248 (33%), Positives = 117/248 (47%)
Frame = +3
Query: 3 VDADWKIINGSRTVLHEYPEIVSITQDGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWL 182
VD D KI+ G + P VS+ G H CGG+L+N V++AAHC
Sbjct: 15 VDDDDKIVGGYTCGANTVPYQVSLNS-GYHFCGGSLINSQWVVSAAHCYKS--------- 64
Query: 183 EYHQVKVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGNDVAVIKFNGFISNYD 362
++V +GE N+N +EG E+ +K+I+HPSY ND+ +IK S
Sbjct: 65 ---GIQVRLGEDNINVVEGNEQFISASKSIVHPSYNSNTL---NNDIMLIKLKSAASLN- 117
Query: 363 VEYIKYVKLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKTYRNV 542
+ + L T S + G +C++ GWG+ G + L+ I+S C Y
Sbjct: 118 -SRVASISLPT-SCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKSAYPG- 174
Query: 543 QFVPSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPTVYTR 722
Q + CAG K +C+GDSGGP+ C GIVS+ S C Q NKP VYT+
Sbjct: 175 QITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKL-----QGIVSWG-SGCAQKNKPGVYTK 228
Query: 723 VSEVAKWI 746
V WI
Sbjct: 229 VCNYVSWI 236
>sp|P26262|KLKB1_MOUSE Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 123 bits (308), Expect = 7e-28
Identities = 92/256 (35%), Positives = 131/256 (51%), Gaps = 9/256 (3%)
Frame = +3
Query: 18 KIINGSRTVLHEYPEIVSITQ---DGEHVCGGTLLNGNTVLTAAHCIWEMRYWTKEWLEY 188
+I+ G+ L E+P VS+ H+CGG+++ VLTAAHC + + Y
Sbjct: 390 RIVGGTNASLGEWPWQVSLQVKLVSQTHLCGGSIIGRQWVLTAAHCF--------DGIPY 441
Query: 189 HQV-KVVIGEYNVNQLEGTEKIYQVTKTIMHPSYKREKAYVDGN-DVAVIKFNGFISNYD 362
V ++ G +++++ ++ + I+H YK +GN D+A+IK + NY
Sbjct: 442 PDVWRIYGGILSLSEITKETPSSRIKELIIHQEYKVS----EGNYDIALIKLQTPL-NY- 495
Query: 363 VEYIKYV----KLDTNSISKPGDRCVVVGWGSMLPDGEDTAEYLQKADLNIVSTEECSKT 530
E+ K + K DTN+I C V GWG GE T LQKA + +V EEC K
Sbjct: 496 TEFQKPICLPSKADTNTIYT---NCWVTGWGYTKEQGE-TQNILQKATIPLVPNEECQKK 551
Query: 531 YRNVQFVPSNICAGSGRLSKSACKGDSGGPLFCRNSFGEQIQVGIVSYSHSPCGQINKPT 710
YR+ ICAG ACKGDSGGPL C++S G VGI S+ CG+ ++P
Sbjct: 552 YRDYVINKQMICAGYKEGGTDACKGDSGGPLVCKHS-GRWQLVGITSWGEG-CGRKDQPG 609
Query: 711 VYTRVSEVAKWIAMNT 758
VYT+VSE WI T
Sbjct: 610 VYTKVSEYMDWILEKT 625
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,175,051
Number of Sequences: 369166
Number of extensions: 1883323
Number of successful extensions: 6751
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5420
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5648
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7454154400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)