Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_K15
(906 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic 306 4e-83
sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic 303 3e-82
sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic 301 1e-81
sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic 298 1e-80
sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2 262 9e-70
sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1 260 3e-69
sp|O48905|MDHC_MEDSA Malate dehydrogenase, cytoplasmic 259 7e-69
sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic 259 9e-69
sp|Q9SML8|MDHC_BETVU Malate dehydrogenase, cytoplasmic 256 8e-68
sp|Q08062|MDHC_MAIZE Malate dehydrogenase, cytoplasmic 255 1e-67
>sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 306 bits (785), Expect = 4e-83
Identities = 157/257 (61%), Positives = 187/257 (72%), Gaps = 3/257 (1%)
Frame = +1
Query: 1 LQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPA 180
L+EAF D+D ALMVGAMPRK+GMER+DLL +NV IFK QGEALDKYAKKTV+V VVGNPA
Sbjct: 74 LKEAFTDIDVALMVGAMPRKQGMERRDLLSSNVKIFKEQGEALDKYAKKTVKVLVVGNPA 133
Query: 181 NTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYP 360
NTNCLIMSKYAPSIPKENF+ALTRLDHNRAI Q+A KAGVP V+ IWGNHSN Q+P
Sbjct: 134 NTNCLIMSKYAPSIPKENFTALTRLDHNRAIYQVAAKAGVPNTCVKNVCIWGNHSNKQFP 193
Query: 361 DFHHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXD 540
D H +V DGK+ + + DE W+K F+ VQ RGAAVI D
Sbjct: 194 DLSHAVVT-KDGKQHPAKELINDEKWVKEVFIPCVQNRGAAVIGLRKLSRAASAAKAIVD 252
Query: 541 HCRDWFHGTK--EWVSMAVYSDGS-YGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGK 711
RDW+ GTK EWVSM+VYS G YG PKD+ FSFPVTI+ G + +V GL++D++SR
Sbjct: 253 QMRDWWFGTKEGEWVSMSVYSTGDHYGAPKDIYFSFPVTIKDGHYKVVDGLSMDEWSRSL 312
Query: 712 LELTGKELIEERDQALA 762
L+ EL++ER+ ALA
Sbjct: 313 FNLSADELVDEREVALA 329
>sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic
Length = 334
Score = 303 bits (777), Expect = 3e-82
Identities = 160/257 (62%), Positives = 189/257 (73%), Gaps = 3/257 (1%)
Frame = +1
Query: 10 AFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTN 189
AF D+D A++VG+MPR++GMERKDLLKANV IFK QG ALDKYAKK+V+V VVGNPANTN
Sbjct: 77 AFKDLDVAILVGSMPRRDGMERKDLLKANVKIFKCQGAALDKYAKKSVKVIVVGNPANTN 136
Query: 190 CLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFH 369
CL SK APSIPKENFS LTRLDHNRA AQIA K GV D V+ IIWGNHS+TQYPD +
Sbjct: 137 CLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVN 196
Query: 370 HGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCR 549
H VK KEV V +AVKD++WLK +F+ TVQ RGAAVI DH R
Sbjct: 197 HAKVK-LQAKEVGVYEAVKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVR 255
Query: 550 DWFHGTK--EWVSMAVYSDG-SYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLEL 720
D + GT E+VSM + SDG SYGVP D+++SFPVTI+ W IV+GL I+DFSR K++L
Sbjct: 256 DIWFGTPEGEFVSMGIISDGNSYGVPDDLLYSFPVTIKDKTWKIVEGLPINDFSREKMDL 315
Query: 721 TGKELIEERDQALAFCS 771
T KEL EE++ A F S
Sbjct: 316 TAKELAEEKETAFEFLS 332
>sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic
Length = 334
Score = 301 bits (772), Expect = 1e-81
Identities = 159/257 (61%), Positives = 189/257 (73%), Gaps = 3/257 (1%)
Frame = +1
Query: 10 AFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTN 189
AF D+D A++VG+MPR+EGMERKDLLKANV IFK QG ALDKYAKK+V+V VVGNPANTN
Sbjct: 77 AFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTN 136
Query: 190 CLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFH 369
CL SK APSIPKENFS LTRLDHNRA AQIA K GV + V+ IIWGNHS+TQYPD +
Sbjct: 137 CLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVN 196
Query: 370 HGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCR 549
H VK GKEV V +A+KD++WLK +F+ TVQ RGAAVI DH R
Sbjct: 197 HAKVK-LQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVR 255
Query: 550 DWFHGTK--EWVSMAVYSDG-SYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLEL 720
D + GT E+VSM V SDG SYGVP D+++SFPV I++ W V+GL I+DFSR K++L
Sbjct: 256 DIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDL 315
Query: 721 TGKELIEERDQALAFCS 771
T KEL EE++ A F S
Sbjct: 316 TAKELTEEKESAFEFLS 332
>sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic
Length = 334
Score = 298 bits (763), Expect = 1e-80
Identities = 157/257 (61%), Positives = 188/257 (73%), Gaps = 3/257 (1%)
Frame = +1
Query: 10 AFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTN 189
AF D+D A++VG+MPR+EGMERKDLLKANV IFK QG AL+KYAKK+V+V VVGNPANTN
Sbjct: 77 AFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGTALEKYAKKSVKVIVVGNPANTN 136
Query: 190 CLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFH 369
CL SK APSIPKENFS LTRLDHNRA +QIA K GV D V+ IIWGNHS+TQYPD +
Sbjct: 137 CLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVN 196
Query: 370 HGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCR 549
H VK GKEV V +A+KD++WLK +F+ TVQ RGAAVI DH R
Sbjct: 197 HAKVK-LQGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAIADHIR 255
Query: 550 DWFHGTK--EWVSMAVYSDG-SYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLEL 720
D + GT E+VSM V SDG SYGVP D+++S PV I++ W V+GL I+DFSR K++L
Sbjct: 256 DIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSLPVVIKNKTWKFVEGLPINDFSREKMDL 315
Query: 721 TGKELIEERDQALAFCS 771
T KEL EE++ A F S
Sbjct: 316 TAKELTEEKETAFEFLS 332
>sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2
Length = 332
Score = 262 bits (670), Expect = 9e-70
Identities = 135/257 (52%), Positives = 173/257 (67%), Gaps = 2/257 (0%)
Frame = +1
Query: 7 EAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANT 186
EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+K+A +V VV NPANT
Sbjct: 77 EACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAPNCKVLVVANPANT 136
Query: 187 NCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDF 366
N LI+ ++APSIP++N + LTRLDHNRA+ Q++ + VP V+ IIWGNHS+TQYPD
Sbjct: 137 NALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVKNVIIWGNHSSTQYPDV 196
Query: 367 HHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHC 546
+H VK G E V + VK++ WL +F+ TVQ RGAA+I DH
Sbjct: 197 NHATVKTSVG-EKPVRELVKNDEWLNGEFISTVQQRGAAIIKARKLSSALSAASSACDHI 255
Query: 547 RDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLEL 720
RDW GT E +VSM VYSDGSY VP +I+SFPVT +G+W IVQGL IDD SR K++L
Sbjct: 256 RDWVVGTPEGTFVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTIVQGLPIDDASRKKMDL 315
Query: 721 TGKELIEERDQALAFCS 771
T +EL EE+D A + S
Sbjct: 316 TAEELKEEKDLAYSCLS 332
>sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1
Length = 332
Score = 260 bits (665), Expect = 3e-69
Identities = 133/252 (52%), Positives = 170/252 (67%), Gaps = 2/252 (0%)
Frame = +1
Query: 22 VDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANTNCLIM 201
V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+K+A +V VV NPANTN LI+
Sbjct: 82 VNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAAPNCKVLVVANPANTNALIL 141
Query: 202 SKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDFHHGLV 381
++APSIP++N S LTRLDHNRA+ QI+ + VP V+ IIWGNHS++QYPD +H V
Sbjct: 142 KEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVKNVIIWGNHSSSQYPDVNHAKV 201
Query: 382 KGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHCRDWFH 561
+ G E V + VKD+ WL +F+ TVQ RGAA+I DH RDW
Sbjct: 202 QTSSG-EKPVRELVKDDAWLDGEFISTVQQRGAAIIKARKLSSALSAASSACDHIRDWVL 260
Query: 562 GTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLELTGKEL 735
GT E +VSM VYSDGSY VP +I+SFPVT +G W IVQGL ID+ SR K++LT +EL
Sbjct: 261 GTPEGTFVSMGVYSDGSYSVPSGLIYSFPVTCRNGDWSIVQGLPIDEVSRKKMDLTAEEL 320
Query: 736 IEERDQALAFCS 771
EE+D A + S
Sbjct: 321 KEEKDLAYSCLS 332
>sp|O48905|MDHC_MEDSA Malate dehydrogenase, cytoplasmic
Length = 332
Score = 259 bits (662), Expect = 7e-69
Identities = 133/252 (52%), Positives = 168/252 (66%), Gaps = 2/252 (0%)
Frame = +1
Query: 7 EAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANT 186
EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+K+A +V VV NPANT
Sbjct: 77 EACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAANCKVLVVANPANT 136
Query: 187 NCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDF 366
N LI+ ++APSIP+ N S LTRLDHNRA+ QI+ + V V+ IIWGNHS+TQYPD
Sbjct: 137 NALILKEFAPSIPERNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDV 196
Query: 367 HHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHC 546
+H V G E V V D+ WL +F+ TVQ RGAA+I DH
Sbjct: 197 NHATVNTPAG-EKPVRQLVSDDAWLNGEFISTVQQRGAAIIKARKLSSALSAASAACDHI 255
Query: 547 RDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLEL 720
RDW GT + +VSM VYSDGSY VP +I+SFPVT +G+W IVQGL+ID+FSR KL+L
Sbjct: 256 RDWVLGTPQGTFVSMGVYSDGSYNVPSGLIYSFPVTCANGEWKIVQGLSIDEFSRKKLDL 315
Query: 721 TGKELIEERDQA 756
T +EL EE++ A
Sbjct: 316 TAEELTEEKNLA 327
>sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 259 bits (661), Expect = 9e-69
Identities = 131/252 (51%), Positives = 167/252 (66%), Gaps = 2/252 (0%)
Frame = +1
Query: 7 EAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANT 186
EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+++A +V VV NPANT
Sbjct: 77 EACKGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPNCKVLVVANPANT 136
Query: 187 NCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDF 366
N LI+ ++APSIP++N S LTRLDHNRA+ QI+ + V V+ IIWGNHS+TQYPD
Sbjct: 137 NALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDV 196
Query: 367 HHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHC 546
+H VK G + V + V D+ WL +F+ TVQ RGAA+I DH
Sbjct: 197 NHATVK-TQGVDKPVRELVADDAWLNGEFITTVQQRGAAIIKARKLSSALSAASSACDHI 255
Query: 547 RDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLEL 720
DW GT E WVSM VYSDGSY VP +I+SFPVT ++G+W IVQGL IDD SR K++
Sbjct: 256 HDWVLGTPEGTWVSMGVYSDGSYNVPAGIIYSFPVTCKNGEWTIVQGLPIDDDSRKKMDA 315
Query: 721 TGKELIEERDQA 756
T EL+EE+ A
Sbjct: 316 TAAELVEEKTLA 327
>sp|Q9SML8|MDHC_BETVU Malate dehydrogenase, cytoplasmic
Length = 332
Score = 256 bits (653), Expect = 8e-68
Identities = 132/255 (51%), Positives = 170/255 (66%), Gaps = 2/255 (0%)
Frame = +1
Query: 7 EAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANT 186
EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL++YA +V VV NPANT
Sbjct: 77 EACKGVNVAVMVGGFPRKEGMERKDVMPKNVSIYKSQASALEQYAAPNCKVLVVANPANT 136
Query: 187 NCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDF 366
N LI+ ++APSIP++N + LTRLDHNRA+ QI+ + V+ IIWGNHS++QYPD
Sbjct: 137 NALILKEFAPSIPEKNITCLTRLDHNRALGQISERLNAQVSDVKNVIIWGNHSSSQYPDV 196
Query: 367 HHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHC 546
+ VK G E +V + V D+ WL +F+ TVQ RGAA+I DH
Sbjct: 197 NPCTVKTGSG-EKAVRELVADDAWLNGEFITTVQQRGAAIIKARKLSSALSAASSACDHI 255
Query: 547 RDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLEL 720
RDW GT E WVSM VYSDGSY VP +I+SFPVT + G+W IVQGL ID+ SR K++
Sbjct: 256 RDWVLGTPEGTWVSMGVYSDGSYNVPAGIIYSFPVTCKDGEWKIVQGLPIDEVSRQKMDA 315
Query: 721 TGKELIEERDQALAF 765
TG EL+EE+ ALA+
Sbjct: 316 TGAELVEEK--ALAY 328
>sp|Q08062|MDHC_MAIZE Malate dehydrogenase, cytoplasmic
Length = 332
Score = 255 bits (651), Expect = 1e-67
Identities = 131/252 (51%), Positives = 167/252 (66%), Gaps = 2/252 (0%)
Frame = +1
Query: 7 EAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTVRVCVVGNPANT 186
EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+ +A +V VV NPANT
Sbjct: 77 EACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEAHAAPNCKVLVVANPANT 136
Query: 187 NCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIWGNHSNTQYPDF 366
N LI+ ++APSIP++N + LTRLDHNRA+ QI+ + V V+ IIWGNHS++QYPD
Sbjct: 137 NALILKEFAPSIPEKNVTCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSSQYPDV 196
Query: 367 HHGLVKGHDGKEVSVLDAVKDETWLKNDFLKTVQTRGAAVIXXXXXXXXXXXXXXXXDHC 546
+H VK G E V + V D+ WL +F+ TVQ RGAA+I DH
Sbjct: 197 NHATVKTSTG-EKPVRELVSDDEWLNGEFITTVQQRGAAIIKARKFSSALSAASSACDHI 255
Query: 547 RDWFHGTKE--WVSMAVYSDGSYGVPKDVIFSFPVTIEHGKWHIVQGLTIDDFSRGKLEL 720
RDW GT E +VSM VYSDGSYGVP +I+SFPVT G+W IVQGL ID+FSR K++
Sbjct: 256 RDWVLGTPEGTFVSMGVYSDGSYGVPSGLIYSFPVTCSGGEWKIVQGLPIDEFSRKKMDA 315
Query: 721 TGKELIEERDQA 756
T +EL EE+ A
Sbjct: 316 TAQELTEEKTLA 327
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 100,001,531
Number of Sequences: 369166
Number of extensions: 1994191
Number of successful extensions: 5208
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4927
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5075
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9174518830
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)