Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_I03
(832 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O95433|AHSA1_HUMAN Activator of 90 kDa heat shock protei... 207 4e-53
sp|Q8BK64|AHSA1_MOUSE Activator of 90 kDa heat shock protei... 205 1e-52
sp|Q12449|AHA1_YEAST Hsp90 co-chaperone AHA1 (Activator of ... 93 8e-19
sp|Q9P782|YNY8_SCHPO Hypothetical protein C1711.08 in chrom... 90 9e-18
sp|P53834|HCH1_YEAST Hsp90 co-chaperone HCH1 (High-copy Hsp... 63 9e-10
sp|Q02224|CENPE_HUMAN Centromeric protein E (CENP-E protein) 33 0.74
sp|Q98QL0|RNR_MYCPU Ribonuclease R (RNase R) (VacB protein ... 33 0.74
sp|O42184|REST_CHICK Restin (Cytoplasmic linker protein 170... 33 0.74
sp|Q8RDZ8|SYE_FUSNN Glutamyl-tRNA synthetase (Glutamate--tR... 33 0.74
sp|Q8RHB5|SYFB_FUSNN Phenylalanyl-tRNA synthetase beta chai... 33 0.96
>sp|O95433|AHSA1_HUMAN Activator of 90 kDa heat shock protein ATPase homolog 1 (AHA1)
(p38)
Length = 338
Score = 207 bits (526), Expect = 4e-53
Identities = 108/266 (40%), Positives = 160/266 (60%), Gaps = 5/266 (1%)
Frame = +2
Query: 47 MAKWGEGDPRWIVEEREDAKNVNNWHWSEKNATQWSKDMFKKLLVGLVIDNNEYFFEITD 226
MAKWGEGDPRWIVEER DA NVNNWHW+E++A+ WS D K L + + + N E E+T+
Sbjct: 1 MAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTE 60
Query: 227 LSKIEGEAIANNRKAKVIVFYEWVIEAEWKGHLKNNINKTEFKGKFDIPNLSEEFDSSEI 406
+SK++GEA NNRK K+I FYEW ++ W G K+ + ++KG +IPNLS+E E+
Sbjct: 61 VSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGV---QYKGHVEIPNLSDENSVDEV 117
Query: 407 DVNVSVESHSNDANQMKDYIRLECSKVIREKLGKYISDLKTEYSKDLILPTKKVSNDGPV 586
+++VS+ D N + ++ E K++RE +G YIS LKTE+++ +ILPT + P
Sbjct: 118 EISVSLAKDEPDTN-LVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDP- 175
Query: 587 VVSNAYKQTMNEIVVSKSHKNS-----SLTFLSLELNEEFFCTPSDLYQVFINSDLVSAF 751
V A K + + S + + + L E F +P +LY+VF +LV AF
Sbjct: 176 VGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAF 235
Query: 752 SRSPSKVHGVVGGEFLMFGDTISGVF 829
+ +P+ + GG+F M +SG F
Sbjct: 236 THAPATLEADRGGKFHMVDGNVSGEF 261
>sp|Q8BK64|AHSA1_MOUSE Activator of 90 kDa heat shock protein ATPase homolog 1 (AHA1)
Length = 338
Score = 205 bits (521), Expect = 1e-52
Identities = 106/265 (40%), Positives = 161/265 (60%), Gaps = 4/265 (1%)
Frame = +2
Query: 47 MAKWGEGDPRWIVEEREDAKNVNNWHWSEKNATQWSKDMFKKLLVGLVIDNNEYFFEITD 226
MAKWGEGDPRWIVEER DA NVNNWHW+E++A+ WS + K L + + ++N E E+T+
Sbjct: 1 MAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTEKLKTLFLAVRVENEEGKCEVTE 60
Query: 227 LSKIEGEAIANNRKAKVIVFYEWVIEAEWKGHLKNNINKTEFKGKFDIPNLSEEFDSSEI 406
++K++GEA NNRK K+I FYEW I+ W G K+ + ++KG +IPNLS+E E+
Sbjct: 61 VNKLDGEASINNRKGKLIFFYEWTIKLNWTGTSKSGV---QYKGHVEIPNLSDENSVDEV 117
Query: 407 DVNVSVESHSNDANQMKDYIRLECSKVIREKLGKYISDLKTEYSKDLILPTKKVSNDGPV 586
+++VS+ D N + ++ + K++RE +G YIS LKTE+++ +ILPT + PV
Sbjct: 118 EISVSLAKDEPDTN-LVALMKEDGVKLLREAVGIYISTLKTEFTQGMILPTVNGESVDPV 176
Query: 587 VVSNAYKQTMN-EIVVSKSHKNS---SLTFLSLELNEEFFCTPSDLYQVFINSDLVSAFS 754
+T + SKS + + L E F +P +LY+VF +LV AF+
Sbjct: 177 GQPALKTETCKAKSAPSKSQAKPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFT 236
Query: 755 RSPSKVHGVVGGEFLMFGDTISGVF 829
+P+ + GG+F M ++G F
Sbjct: 237 HAPAALEADRGGKFHMVDGNVTGEF 261
>sp|Q12449|AHA1_YEAST Hsp90 co-chaperone AHA1 (Activator of Hsp90 ATPase protein 1)
Length = 350
Score = 93.2 bits (230), Expect = 8e-19
Identities = 61/241 (25%), Positives = 116/241 (48%), Gaps = 23/241 (9%)
Frame = +2
Query: 107 NVNNWHWSEKNATQWSKDMFKKLLVGLVIDN--NEYFFEITDLSKIEGEAIANNRKAKVI 280
N NNWHW +KN W+K+ FK+ LVG+ + ++ + +I +S IEG+ N RK KVI
Sbjct: 5 NPNNWHWVDKNCIGWAKEYFKQKLVGVEAGSVKDKKYAKIKSVSSIEGDCEVNQRKGKVI 64
Query: 281 VFYEWVIEAEWKGHLKNNINKT-EFKGKFDIPNLSEEFDSSEIDVNVSVESHSNDANQMK 457
++ I +GH+ + F+G ++P ++ + ++S ++S+ +++ ++ K
Sbjct: 65 SLFDLKITVLIEGHVDSKDGSALPFEGSINVPEVAFDSEASSYQFDISIFKETSELSEAK 124
Query: 458 DYIRLECSKVIREKLGKYISDLKTEYSKDLILPTKKVSND---------GPVVVSNAYKQ 610
IR E +R+ ++ DL + D+ +P +V ++ + +A K
Sbjct: 125 PLIRSELLPKLRQIFQQFGKDLLATHGNDIQVPESQVKSNYTRGNQKSSFTEIKDSASKP 184
Query: 611 TMNEIVVS-----------KSHKNSSLTFLSLELNEEFFCTPSDLYQVFINSDLVSAFSR 757
N + S K +N S S+ L F S+LY+ F++ + A++R
Sbjct: 185 KKNALPSSTSTSAPVSSTNKVPQNGSGNSTSIYLEPTFNVPSSELYETFLDKQRILAWTR 244
Query: 758 S 760
S
Sbjct: 245 S 245
>sp|Q9P782|YNY8_SCHPO Hypothetical protein C1711.08 in chromosome II
Length = 336
Score = 89.7 bits (221), Expect = 9e-18
Identities = 62/257 (24%), Positives = 114/257 (44%), Gaps = 16/257 (6%)
Frame = +2
Query: 107 NVNNWHWSEKNATQWSKDMFKKLLVGLVIDNNEYFFEITDLSKIEGEAIANNRKAKVIVF 286
N NNWHW+ K+ WS + F K L + IT ++ EG+ + RK KVI
Sbjct: 7 NPNNWHWTSKDCRVWSHEYFNKELPKIQASEGPTSARITQVNSCEGDVDVSMRKRKVITI 66
Query: 287 YEWVIEAEWKGHLKNNINKTEFKGKFDIPNLSEEFDSSEIDVNVSVESHSNDANQMKDYI 466
++ I+ E+KG K+ + T G P LS + S+ ++ + S S + +K+ +
Sbjct: 67 FDLKIQMEFKGETKDGVEAT---GSITCPELSYDLGYSDYVFDIDIYSASKEKEPIKELV 123
Query: 467 RLECSKVIREKLGKYISDLKTEYSKDLILPTKKVSNDGP--VVVSNAYKQTMNEIVVSK- 637
R + IR+ + L + D+ L T++ + + + V +++KQ + S
Sbjct: 124 REKIIPQIRQLFSGFSQVLLQTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNK 183
Query: 638 -------------SHKNSSLTFLSLELNEEFFCTPSDLYQVFINSDLVSAFSRSPSKVHG 778
S ++ + + N F ++LY F++ V+A+SR+P ++
Sbjct: 184 GTTTVAAGSGSDGSRVSAVVNTADISENYTFDAPANELYATFLDPARVAAWSRAPPQLDV 243
Query: 779 VVGGEFLMFGDTISGVF 829
G F +F + G F
Sbjct: 244 RPQGAFSLFHGNVVGKF 260
>sp|P53834|HCH1_YEAST Hsp90 co-chaperone HCH1 (High-copy Hsp90 suppressor protein 1)
Length = 153
Score = 63.2 bits (152), Expect = 9e-10
Identities = 48/155 (30%), Positives = 71/155 (45%), Gaps = 8/155 (5%)
Frame = +2
Query: 107 NVNNWHWSEKNATQWSKDMFKKLLVGL--VIDNNEYFFEITDLSKIEGEAIANNRKAKVI 280
N NNWHW +KN WSKD L L V + + E+T +S I G++ + RK K I
Sbjct: 5 NPNNWHWVDKNTLPWSKDYLNGKLTSLSTVSSDGKSKIELTQVSSITGDSNVSQRKGKPI 64
Query: 281 VFYEWVIEAEWK-GHLKNNINKTE-----FKGKFDIPNLSEEFDSSEIDVNVSVESHSND 442
+++ + K +L N + + GK +IP EF E D+ + S
Sbjct: 65 CYFDLQLSMNVKVTNLDTNKDDEDDDGILADGKLEIP----EFMHDESDIPIL----SQG 116
Query: 443 ANQMKDYIRLECSKVIREKLGKYISDLKTEYSKDL 547
+ +R E + E L KY DL E+SKD+
Sbjct: 117 FDAFDGLVRSEFVPKVVETLLKYQDDLIKEHSKDI 151
>sp|Q02224|CENPE_HUMAN Centromeric protein E (CENP-E protein)
Length = 2663
Score = 33.5 bits (75), Expect = 0.74
Identities = 42/161 (26%), Positives = 63/161 (39%), Gaps = 12/161 (7%)
Frame = +2
Query: 233 KIEGEAIANNRKAKVIVFYEWVIEAEWKGHLKNNINKTEFKGKFDIPNLSEEFDSSEIDV 412
K E + + +NRK EW+ LKN I K + I ++ F++ I +
Sbjct: 2217 KTEFQQVLSNRKEMTQFLEEWLNTRFDIEKLKNGIQKENDR----ICQVNNFFNNRIIAI 2272
Query: 413 -NVSVESHSNDANQMKDYIR-LECSKVIREKLGKYISDLKTEYSKDLILPTKKVSNDGPV 586
N S E A K++ + L+ K EKL K LKT + + N P
Sbjct: 2273 MNESTEFEERSATISKEWEQDLKSLKEKNEKLFKNYQTLKTSLASGAQVNPTTQDNKNPH 2332
Query: 587 VVSNAYKQT----------MNEIVVSKSHKNSSLTFLSLEL 679
V S A + T ++E S HK S + + EL
Sbjct: 2333 VTSRATQLTTEKIRELENSLHEAKESAMHKESKIIKMQKEL 2373
>sp|Q98QL0|RNR_MYCPU Ribonuclease R (RNase R) (VacB protein homolog)
Length = 725
Score = 33.5 bits (75), Expect = 0.74
Identities = 50/213 (23%), Positives = 84/213 (39%), Gaps = 44/213 (20%)
Frame = +2
Query: 143 TQWSKDMFKKLLVGLVIDNNE-YFFEITDLSKIEGEAIANNRKAKVI------------- 280
+Q SK +F L GL++ NN+ F ++ ++ KI+G +NR I
Sbjct: 32 SQLSKALFSLLDQGLIVKNNDGNFIKVNEIKKIQGIFKQSNRNFAFIETADEQSYFVAKA 91
Query: 281 -------------VFYEWVIEAEWKGHLKNNINKT---EFKGKFDIPNLSEEFDSSE--- 403
+ YE E + K + I K E G I N + F++ E
Sbjct: 92 HFNGAINSFEVEAIVYESPFEKDKKYAVVKKILKNTHPEIIGFIKISNGIKYFNAFEESF 151
Query: 404 ------IDVNVSVESHSNDANQMKDYIRLECSKVIREKL----GKYISDLKTE-YSKDLI 550
+D N+ VE D I + K++ +K+ K +S LK+ Y D++
Sbjct: 152 RSYKFFVDQNIDVEE--------DDVILVVVEKIVDQKIYVNFVKKVSTLKSNFYQIDIV 203
Query: 551 LPTKKVSNDGPVVVSNAYKQTMNEIVVSKSHKN 649
L K D P V + + + V+ K ++N
Sbjct: 204 LEKSKTIIDFPEDVLDE-SALIEDHVIEKDYQN 235
>sp|O42184|REST_CHICK Restin (Cytoplasmic linker protein 170) (CLIP-170)
Length = 1433
Score = 33.5 bits (75), Expect = 0.74
Identities = 29/120 (24%), Positives = 56/120 (46%), Gaps = 3/120 (2%)
Frame = +2
Query: 335 INKTEFKGKFDIPNLSEEFDSSEIDV-NVSVESHSNDANQMKDYIRLECSKVIREKLGKY 511
+ K +GK +I LSE+ ++E + N+ E SN +++ E + EK
Sbjct: 769 LQKANSEGKLEIQKLSEQLQAAEKQIQNLETEKVSNLTKELQG---KEQKLLDLEKNLSA 825
Query: 512 ISDLKTEYSKDLILPTKKVSN--DGPVVVSNAYKQTMNEIVVSKSHKNSSLTFLSLELNE 685
++ +K K+L L +K ++ DG A ++T+N++ + K +S EL +
Sbjct: 826 VNQVKDSLEKELQLLKEKFTSAVDGAENAQRAMQETINKL----NQKEEQFALMSSELEQ 881
>sp|Q8RDZ8|SYE_FUSNN Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
Length = 510
Score = 33.5 bits (75), Expect = 0.74
Identities = 50/206 (24%), Positives = 86/206 (41%), Gaps = 13/206 (6%)
Frame = +2
Query: 5 EGFS*FLFIKFYLIMAKWGEGDPRWIVEEREDAKNVNNWHWSEKNATQWSKDMFKKLLVG 184
EG FL + Y +G+G + ++E +D N++ +F + +G
Sbjct: 277 EGLVNFLGLMGY----SYGDGQEIFTLQEFKDNFNIDK--------VTLGGPVFDLVKLG 324
Query: 185 LVIDNNEYFFEITDLSKI-------EGEAIANNRKAKVIVFYEWVIEAEWKG-----HLK 328
V + + ++ +L+++ EG N K + V+ E +G L
Sbjct: 325 WVNNQHMKMKDLGELTRLTIPFFVNEGYLTNENVSEKEFETLKKVVGIEREGAKTLQELA 384
Query: 329 NNINKTEFKGKFDIPNLSEEFDSSEIDVNVSVESHSNDANQMKDYIRLECSKVIREKLGK 508
N +K F +F +P L E+ D E SVE N +KD I L+ K+ EKL K
Sbjct: 385 KN-SKFFFVDEFSLPELREDMDKKE---RKSVERL---LNSLKDEIGLKSIKLFIEKLEK 437
Query: 509 YI-SDLKTEYSKDLILPTKKVSNDGP 583
+ ++ E +KDL+ +GP
Sbjct: 438 WNGNEFTAEQAKDLLHSLLDDLQEGP 463
>sp|Q8RHB5|SYFB_FUSNN Phenylalanyl-tRNA synthetase beta chain (Phenylalanine--tRNA ligase
beta chain) (PheRS)
Length = 798
Score = 33.1 bits (74), Expect = 0.96
Identities = 37/167 (22%), Positives = 66/167 (39%), Gaps = 8/167 (4%)
Frame = +2
Query: 353 KGKFDIPNLSEEFDSSEIDVNVSVESHSNDANQMKDYIRLECSKVIREKLGKYISDLKTE 532
K K+ + ++E +S V V +E D ++ K Y +G+ I ++K +
Sbjct: 186 KVKYPMIEITETIESINTMVKVDIE----DKDRCKRY------------MGRVIKNVKVQ 229
Query: 533 YSKDLILPTKKVSNDGPV--------VVSNAYKQTMNEIVVSKSHKNSSLTFLSLELNEE 688
S D + + P+ V Y Q M+ + K N +T + + NEE
Sbjct: 230 ESPDWLKSRIRAMGLNPINNIVDITNFVMFEYNQPMHAFDLDKLEGN--ITIRAAKKNEE 287
Query: 689 FFCTPSDLYQVFINSDLVSAFSRSPSKVHGVVGGEFLMFGDTISGVF 829
T + +V N +LV A + GV+GG+ + +F
Sbjct: 288 I-TTLDGVDRVLKNGELVIADDEKAIAIAGVIGGQNTQIDNETKNIF 333
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,456,502
Number of Sequences: 369166
Number of extensions: 1650992
Number of successful extensions: 4967
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4811
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4957
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8052550455
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)