Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_H14
(504 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Z0J0|NPC2_MOUSE Epididymal secretory protein E1 precur... 86 5e-17
sp|O97763|NPC2_PIG Epididymal secretory protein E1 precurso... 85 9e-17
sp|P61917|NPC2_PANTR Epididymal secretory protein E1 precur... 84 1e-16
sp|P79345|NPC2_BOVIN Epididymal secretory protein E1 precur... 82 6e-16
sp|Q9DGJ3|NPC2_BRARE Epididymal secretory protein E1 precur... 77 3e-14
sp|Q28895|NPC2_CANFA Epididymal secretory protein E1 precur... 69 5e-12
sp|Q9VQ62|NPC2_DROME Hypothetical protein NPC2 precursor (N... 69 8e-12
sp|Q25481|ES16_MANSE Ecdysteroid regulated 16 kDa protein p... 57 3e-08
sp|P27236|DCP_SALTY Peptidyl-dipeptidase dcp (Dipeptidyl ca... 29 5.6
sp|Q25158|OPSC2_HEMSA Compound eye opsin BCRH2 29 7.3
>sp|Q9Z0J0|NPC2_MOUSE Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (mE1)
Length = 149
Score = 85.9 bits (211), Expect = 5e-17
Identities = 44/143 (30%), Positives = 68/143 (47%)
Frame = +2
Query: 5 ILGLSFINISLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQITIKYKSNENX 184
IL L+ + S EP +KDCGSK G+++ V ++PC T PCQL KG + I + S
Sbjct: 8 ILLLALVAASQAEPLHFKDCGSKVGVIKEVNVSPCPTDPCQLHKGQSYSVNITFTSGTQS 67
Query: 185 XXXXXXXXXXXXIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTI 364
+PF +P+ D + S Y N + + YPS+++ +
Sbjct: 68 QNSTALVHGILEGIRVPFPIPEPDGCKSGINCPIQKDKVYS-YLNKLPVKNEYPSIKLVV 126
Query: 365 TWELIDADGNDIVCVQIPTSIVS 433
W+L D N++ C +IP I S
Sbjct: 127 EWKLEDDKKNNLFCWEIPVQITS 149
>sp|O97763|NPC2_PIG Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (16 kDa secretory protein)
Length = 149
Score = 85.1 bits (209), Expect = 9e-17
Identities = 43/134 (32%), Positives = 64/134 (47%)
Frame = +2
Query: 32 SLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQITIKYKSNENXXXXXXXXXX 211
+L EP ++DCGS G+++ V + PC TQPCQL KG + + + SN
Sbjct: 17 ALAEPVHFRDCGSGVGVIKEVNVNPCPTQPCQLHKGQSYSVNVTFTSNTQSKGSKAVVHG 76
Query: 212 XXXIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWELIDADG 391
+PF +PD D QT S Y N + + YPS+++ + W+L D +
Sbjct: 77 IVMGVPIPFPIPDPDGCKSGINCPIQKDQTYS-YLNKLPVKAEYPSIKLVVEWKLQDDND 135
Query: 392 NDIVCVQIPTSIVS 433
+ C QIP I S
Sbjct: 136 QCLFCWQIPVQIES 149
>sp|P61917|NPC2_PANTR Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (EPI-1)
sp|P61918|NPC2_MACFA Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (Epididymal secretory protein 14.6)
(ESP14.6)
sp|P61916|NPC2_HUMAN Epididymal secretory protein E1 precursor (Niemann-Pick disease
type C2 protein) (hE1)
Length = 151
Score = 84.3 bits (207), Expect = 1e-16
Identities = 42/140 (30%), Positives = 66/140 (47%)
Frame = +2
Query: 14 LSFINISLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQITIKYKSNENXXXX 193
L+ + EP +KDCGS G+++ V ++PC TQPCQL KG + + + SN
Sbjct: 11 LALSTAAQAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSS 70
Query: 194 XXXXXXXXXIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWE 373
+PF +P+ D +T S Y N + + YPS+++ + W+
Sbjct: 71 KAVVHGILMGVPVPFPIPEPDGCKSGINCPIQKDKTYS-YLNKLPVKSEYPSIKLVVEWQ 129
Query: 374 LIDADGNDIVCVQIPTSIVS 433
L D + C +IP IVS
Sbjct: 130 LQDDKNQSLFCWEIPVQIVS 149
>sp|P79345|NPC2_BOVIN Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (EPV20)
Length = 149
Score = 82.4 bits (202), Expect = 6e-16
Identities = 39/132 (29%), Positives = 63/132 (47%)
Frame = +2
Query: 32 SLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQITIKYKSNENXXXXXXXXXX 211
+L EP +KDCGS G+++ V ++PC TQPC+L +G + + + SN
Sbjct: 17 ALAEPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNTQSQSSKAVVHG 76
Query: 212 XXXIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWELIDADG 391
+PF +P++D +T + Y N + + YPS++V + WEL D
Sbjct: 77 IVMGIPVPFPIPESDGCKSGIRCPIEKDKTYN-YVNKLPVKNEYPSIKVVVEWELTDDKN 135
Query: 392 NDIVCVQIPTSI 427
C QIP +
Sbjct: 136 QRFFCWQIPIEV 147
>sp|Q9DGJ3|NPC2_BRARE Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (16.5 kDa secretory protein)
Length = 149
Score = 76.6 bits (187), Expect = 3e-14
Identities = 40/140 (28%), Positives = 62/140 (44%)
Frame = +2
Query: 14 LSFINISLTEPTIYKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQITIKYKSNENXXXX 193
LSF+ + +P + DCGS G V V+I PCS QPC+L KG + + + S
Sbjct: 11 LSFLAYTCADPVKFVDCGSVDGKVVQVDIKPCSQQPCKLHKGQSYTVNVTFSSGVESQTS 70
Query: 194 XXXXXXXXXIFNLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWE 373
+PF +P +D Q Y + + YP+++V + WE
Sbjct: 71 KAVVHGVLAGVPVPFPIPIDD-GCKSGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWE 129
Query: 374 LIDADGNDIVCVQIPTSIVS 433
L D D+ C++ P IV+
Sbjct: 130 LRDDSSKDLFCIKFPVQIVN 149
>sp|Q28895|NPC2_CANFA Epididymal secretory protein E1 precursor (Niemann Pick type C2
protein homolog) (cE1)
Length = 149
Score = 69.3 bits (168), Expect = 5e-12
Identities = 33/125 (26%), Positives = 57/125 (45%)
Frame = +2
Query: 53 YKDCGSKKGIVQSVEITPCSTQPCQLKKGTKPQITIKYKSNENXXXXXXXXXXXXXIFNL 232
+KDCGS G+++ + + PC QPC+L KG + + + SN +
Sbjct: 24 FKDCGSAVGVIKELNVNPCPAQPCKLHKGQSYSVNVTFTSNIPSQSSKAVVHGIVLGVAV 83
Query: 233 PFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWELIDADGNDIVCVQ 412
PF +P+ D +T S Y N + + YPS+++ + W L+ + + C +
Sbjct: 84 PFPIPEADGCKSGINCPIQKDKTYS-YLNKLPVKNEYPSIKLVVQWMLLGDNNQHLFCWE 142
Query: 413 IPTSI 427
IP I
Sbjct: 143 IPVQI 147
>sp|Q9VQ62|NPC2_DROME Hypothetical protein NPC2 precursor (Niemann Pick type C2 protein
homolog)
Length = 148
Score = 68.6 bits (166), Expect = 8e-12
Identities = 40/127 (31%), Positives = 54/127 (42%), Gaps = 2/127 (1%)
Frame = +2
Query: 53 YKDCGSKKGIVQSVEITPCSTQP--CQLKKGTKPQITIKYKSNENXXXXXXXXXXXXXIF 226
+ DCGSK G V I C T C LK+ T +I + E
Sbjct: 21 FSDCGSKTGKFTRVAIEGCDTTKAECILKRNTTVSFSIDFALAEEATAVKTVVHGKVLGI 80
Query: 227 NLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWELIDADGNDIVC 406
+PF L + D + YT ++ +L+ YP V V + WEL D DG DI+C
Sbjct: 81 EMPFPLANPDACVDSGLKCPLEKDESYRYTATLPVLRSYPKVSVLVKWELQDQDGADIIC 140
Query: 407 VQIPTSI 427
V+IP I
Sbjct: 141 VEIPAKI 147
>sp|Q25481|ES16_MANSE Ecdysteroid regulated 16 kDa protein precursor
Length = 145
Score = 56.6 bits (135), Expect = 3e-08
Identities = 36/127 (28%), Positives = 53/127 (41%), Gaps = 2/127 (1%)
Frame = +2
Query: 53 YKDCGSKKGIVQSVEIT--PCSTQPCQLKKGTKPQITIKYKSNENXXXXXXXXXXXXXIF 226
Y DCGSK VQSV ++ P + + C LK+ + I+I + +
Sbjct: 19 YTDCGSKLATVQSVGVSGWPENARECVLKRNSNVTISIDFSPTTDVSAITTEVHGVIMSL 78
Query: 227 NLPFSLPDNDLXXXXXXXXXXXXQTNSTYTNSIDILKYYPSVRVTITWELIDADGNDIVC 406
+PF D + Y ++ +LK YP V V + WEL D D+VC
Sbjct: 79 PVPFPCRSPDACKDNGLTCPIKAGVVANYKTTLPVLKSYPKVSVDVKWEL-KKDEEDLVC 137
Query: 407 VQIPTSI 427
+ IP I
Sbjct: 138 ILIPARI 144
>sp|P27236|DCP_SALTY Peptidyl-dipeptidase dcp (Dipeptidyl carboxypeptidase)
Length = 680
Score = 29.3 bits (64), Expect = 5.6
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 308 TYTNSIDILKYYPSVRVTITWELIDADG 391
T+ DI Y+P VRV WE+ D+DG
Sbjct: 380 TFVERFDIPVYHPDVRV---WEIFDSDG 404
>sp|Q25158|OPSC2_HEMSA Compound eye opsin BCRH2
Length = 377
Score = 28.9 bits (63), Expect = 7.3
Identities = 11/31 (35%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
Frame = -1
Query: 180 FSLDLYLIVI-CGFVPFFNWHGWVLQGVIST 91
F+L ++I I C PFF W ++L+G++ +
Sbjct: 174 FALISWVIAIGCALPPFFGWGNYILEGILDS 204
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,072,218
Number of Sequences: 369166
Number of extensions: 907861
Number of successful extensions: 1940
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1931
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3218280650
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)