Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_G23
(885 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9V3Q6|M3K7_DROME Mitogen-activated protein kinase kinas... 175 1e-43
sp|P83104|M3LK7_DROME Putative mitogen-activated protein ki... 156 8e-38
sp|Q62073|M3K7_MOUSE Mitogen-activated protein kinase kinas... 144 3e-34
sp|O43318|M3K7_HUMAN Mitogen-activated protein kinase kinas... 144 3e-34
sp|Q9ESL4|MLTK_MOUSE Mitogen-activated protein kinase kinas... 126 7e-29
sp|Q9NYL2|MLTK_HUMAN Mitogen-activated protein kinase kinas... 126 9e-29
sp|Q02779|M3K10_HUMAN Mitogen-activated protein kinase kina... 111 3e-24
sp|P80192|M3K9_HUMAN Mitogen-activated protein kinase kinas... 110 7e-24
sp|Q5TCX8|M3KL4_HUMAN Mitogen-activated protein kinase kina... 107 4e-23
sp|Q05609|CTR1_ARATH Serine/threonine-protein kinase CTR1 103 6e-22
>sp|Q9V3Q6|M3K7_DROME Mitogen-activated protein kinase kinase kinase 7
(TGF-beta-activated kinase 1) (dTAK1)
Length = 678
Score = 175 bits (444), Expect = 1e-43
Identities = 95/243 (39%), Positives = 139/243 (57%)
Frame = +2
Query: 134 GSYGSVQRILLQNTECAVKYFRVREHYQKNLAEVNSLSICDHANIVKLFAAGPDLELQKL 313
GSYG V + + ++ AVK F + EV LS H NI+ L Q+
Sbjct: 28 GSYGVVCKAVWRDKLVAVKEFFASAEQKDIEKEVKQLSRVKHPNIIALHGIS---SYQQA 84
Query: 314 HYYIMEFAPNGSLNELLHEQMEVNYSLAQGLNWVHQCTKGVKYLHTYFDNPILHRDIKPA 493
Y IMEFA GSL+ LH +++ YSLA ++W QC +G+ YLH P++HRD+KP
Sbjct: 85 TYLIMEFAEGGSLHNFLHGKVKPAYSLAHAMSWARQCAEGLAYLHAMTPKPLIHRDVKPL 144
Query: 494 NLLLFNSGTVVKLCDFGNVKSVQQTENTADSGTYVYMAPEVMNSVKDRKAYYNEKCDVYS 673
NLLL N G +K+CDFG V + T T + G+ +MAPEV K Y EKCD++S
Sbjct: 145 NLLLTNKGRNLKICDFGTVAD-KSTMMTNNRGSAAWMAPEVFEGSK-----YTEKCDIFS 198
Query: 674 IAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKERLDDIPGCPEKLQRLIKCCWKHQYKER 853
AI++WE+L R++PFK N ++I + K ER + CP++++ L+ CWK ++R
Sbjct: 199 WAIVLWEVLSRKQPFKGIDNAYTI-QWKIYKGERPPLLTTCPKRIEDLMTACWKTVPEDR 257
Query: 854 PNI 862
P++
Sbjct: 258 PSM 260
>sp|P83104|M3LK7_DROME Putative mitogen-activated protein kinase kinase kinase 7-like
Length = 393
Score = 156 bits (394), Expect = 8e-38
Identities = 92/275 (33%), Positives = 150/275 (54%), Gaps = 7/275 (2%)
Frame = +2
Query: 68 VIKNIDIDLSKDIVKDPDFFKGGSYGSVQRILLQNTECAVKYFRVREHYQKNLAE--VNS 241
++K +D ++ F GS G+V++ QN E AVK F E K AE +
Sbjct: 1 MVKQVDF---AEVKLSEKFLGAGSGGAVRKATFQNQEIAVKIFDFLEETIKKNAEREITH 57
Query: 242 LSICDHANIVKLFAAGPDLELQKLHYYIMEFAPNGSLNELLHEQMEVNYSLAQGLNWVHQ 421
LS DH N++++ + K Y +ME+ GSL+ L+ + Y++ Q + W Q
Sbjct: 58 LSEIDHENVIRVIGRASN---GKKDYLLMEYLEEGSLHNYLYGDDKWEYTVEQAVRWALQ 114
Query: 422 CTKGVKYLHTYFDNPILHRDIKPANLLLFNSGTVVKLCDFGNVKSVQQTENTADSGTYVY 601
C K + YLH+ D PI+HRDIKP N+LL+N +K+CDFG + + T GT Y
Sbjct: 115 CAKALAYLHS-LDRPIVHRDIKPQNMLLYNQHEDLKICDFG-LATDMSNNKTDMQGTLRY 172
Query: 602 MAPEVMNSVKDRKAYYNEKCDVYSIAIIIWEILVRQKPF---KNAPNVFSIMNTVVL-KK 769
MAPE + +K Y KCDVYS I++WE++ RQ P+ +N + ++IM + +K
Sbjct: 173 MAPEAIKHLK-----YTAKCDVYSFGIMLWELMTRQLPYSHLENPNSQYAIMKAISSGEK 227
Query: 770 ERLDDI-PGCPEKLQRLIKCCWKHQYKERPNISDV 871
++ + CPE +++L++CC ++RP++ ++
Sbjct: 228 LPMEAVRSDCPEGIKQLMECCMDINPEKRPSMKEI 262
>sp|Q62073|M3K7_MOUSE Mitogen-activated protein kinase kinase kinase 7 (Transforming
growth factor-beta-activated kinase 1)
(TGF-beta-activated kinase 1)
Length = 579
Score = 144 bits (363), Expect = 3e-34
Identities = 83/247 (33%), Positives = 131/247 (53%), Gaps = 1/247 (0%)
Frame = +2
Query: 134 GSYGSVQRILLQNTECAVKYFRVREHYQKNLAEVNSLSICDHANIVKLFAAGPDLELQKL 313
G++G V + + + A+K + + E+ LS +H NIVKL+ A +
Sbjct: 45 GAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVC--- 101
Query: 314 HYYIMEFAPNGSLNELLHEQMEVNY-SLAQGLNWVHQCTKGVKYLHTYFDNPILHRDIKP 490
+ME+A GSL +LH + Y + A ++W QC++GV YLH+ ++HRD+KP
Sbjct: 102 --LVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKP 159
Query: 491 ANLLLFNSGTVVKLCDFGNVKSVQQTENTADSGTYVYMAPEVMNSVKDRKAYYNEKCDVY 670
NLLL GTV+K+CDFG + QT T + G+ +MAPEV + Y+EKCDV+
Sbjct: 160 PNLLLVAGGTVLKICDFGTACDI-QTHMTNNKGSAAWMAPEVFEG-----SNYSEKCDVF 213
Query: 671 SIAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKERLDDIPGCPEKLQRLIKCCWKHQYKE 850
S II+WE++ R+KPF + V R I P+ ++ L+ CW +
Sbjct: 214 SWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQ 273
Query: 851 RPNISDV 871
RP++ ++
Sbjct: 274 RPSMEEI 280
>sp|O43318|M3K7_HUMAN Mitogen-activated protein kinase kinase kinase 7 (Transforming
growth factor-beta-activated kinase 1)
(TGF-beta-activated kinase 1)
Length = 606
Score = 144 bits (363), Expect = 3e-34
Identities = 83/247 (33%), Positives = 131/247 (53%), Gaps = 1/247 (0%)
Frame = +2
Query: 134 GSYGSVQRILLQNTECAVKYFRVREHYQKNLAEVNSLSICDHANIVKLFAAGPDLELQKL 313
G++G V + + + A+K + + E+ LS +H NIVKL+ A +
Sbjct: 45 GAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVC--- 101
Query: 314 HYYIMEFAPNGSLNELLHEQMEVNY-SLAQGLNWVHQCTKGVKYLHTYFDNPILHRDIKP 490
+ME+A GSL +LH + Y + A ++W QC++GV YLH+ ++HRD+KP
Sbjct: 102 --LVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKP 159
Query: 491 ANLLLFNSGTVVKLCDFGNVKSVQQTENTADSGTYVYMAPEVMNSVKDRKAYYNEKCDVY 670
NLLL GTV+K+CDFG + QT T + G+ +MAPEV + Y+EKCDV+
Sbjct: 160 PNLLLVAGGTVLKICDFGTACDI-QTHMTNNKGSAAWMAPEVFEG-----SNYSEKCDVF 213
Query: 671 SIAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKERLDDIPGCPEKLQRLIKCCWKHQYKE 850
S II+WE++ R+KPF + V R I P+ ++ L+ CW +
Sbjct: 214 SWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQ 273
Query: 851 RPNISDV 871
RP++ ++
Sbjct: 274 RPSMEEI 280
>sp|Q9ESL4|MLTK_MOUSE Mitogen-activated protein kinase kinase kinase MLT (MLK-like
mitogen-activated protein triple kinase) (Leucine zipper
and sterile alpha motif kinase ZAK) (Sterile-alpha motif
and leucine zipper containing kinase AZK) (Mixed lineage
kinase-related kinase MRK)
Length = 802
Score = 126 bits (317), Expect = 7e-29
Identities = 83/259 (32%), Positives = 130/259 (50%), Gaps = 6/259 (2%)
Frame = +2
Query: 113 DPDFFK---GGSYGSVQRI--LLQNTECAVKYFRVREHYQKNLAEVNSLSICDHANIVKL 277
D FF+ GGS+GSV R + Q+ E AVK E E LS+ H NI++
Sbjct: 15 DLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEK------EAEILSVLSHRNIIQF 68
Query: 278 FAAGPDLELQKLHYYIM-EFAPNGSLNELLHEQMEVNYSLAQGLNWVHQCTKGVKYLHTY 454
+ + L+ +Y I+ E+A GSL + ++ + + W KG+ YLH
Sbjct: 69 YG----VILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHME 124
Query: 455 FDNPILHRDIKPANLLLFNSGTVVKLCDFGNVKSVQQTENTADSGTYVYMAPEVMNSVKD 634
++HRD+K N+++ G V+K+CDFG + T + + GT+ +MAPEV+ S+
Sbjct: 125 APVKVIHRDLKSRNVVIAADG-VLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLP- 182
Query: 635 RKAYYNEKCDVYSIAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKERLDDIPGCPEKLQR 814
+E CD YS +++WE+L R+ PFK + + VV K ERL CP
Sbjct: 183 ----VSETCDTYSYGVVLWEMLTREVPFKGLEGL-QVAWLVVEKNERLTIPSSCPRSFAE 237
Query: 815 LIKCCWKHQYKERPNISDV 871
L+ CW+ K+RP+ +
Sbjct: 238 LLHQCWEADAKKRPSFKQI 256
>sp|Q9NYL2|MLTK_HUMAN Mitogen-activated protein kinase kinase kinase MLT (MLK-like
mitogen-activated protein triple kinase) (Leucine zipper
and sterile alpha motif kinase ZAK) (Sterile-alpha motif
and leucine zipper containing kinase AZK) (Mixed lineage
kinase-related kinase MRK) (Cervical cancer suppressor
gene-4 protein)
Length = 800
Score = 126 bits (316), Expect = 9e-29
Identities = 83/259 (32%), Positives = 130/259 (50%), Gaps = 6/259 (2%)
Frame = +2
Query: 113 DPDFFK---GGSYGSVQRI--LLQNTECAVKYFRVREHYQKNLAEVNSLSICDHANIVKL 277
D FF+ GGS+GSV R + Q+ E AVK E E LS+ H NI++
Sbjct: 15 DLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEK------EAEILSVLSHRNIIQF 68
Query: 278 FAAGPDLELQKLHYYIM-EFAPNGSLNELLHEQMEVNYSLAQGLNWVHQCTKGVKYLHTY 454
+ + L+ +Y I+ E+A GSL + ++ + + W KG+ YLH
Sbjct: 69 YG----VILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHME 124
Query: 455 FDNPILHRDIKPANLLLFNSGTVVKLCDFGNVKSVQQTENTADSGTYVYMAPEVMNSVKD 634
++HRD+K N+++ G V+K+CDFG + T + + GT+ +MAPEV+ S+
Sbjct: 125 APVKVIHRDLKSRNVVIAADG-VLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLP- 182
Query: 635 RKAYYNEKCDVYSIAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKERLDDIPGCPEKLQR 814
+E CD YS +++WE+L R+ PFK + + VV K ERL CP
Sbjct: 183 ----VSETCDTYSYGVVLWEMLTREVPFKGLEGL-QVAWLVVEKNERLTIPSSCPRSFAE 237
Query: 815 LIKCCWKHQYKERPNISDV 871
L+ CW+ K+RP+ +
Sbjct: 238 LLHQCWEADAKKRPSFKQI 256
>sp|Q02779|M3K10_HUMAN Mitogen-activated protein kinase kinase kinase 10 (Mixed lineage
kinase 2) (Protein kinase MST)
Length = 954
Score = 111 bits (277), Expect = 3e-24
Identities = 77/266 (28%), Positives = 126/266 (47%), Gaps = 16/266 (6%)
Frame = +2
Query: 134 GSYGSVQRILLQNTECAVKYFRVREHYQKNLA------EVNSLSICDHANIVKLFAA--- 286
G +G V R L + E AVK R+ + E H NI+ L A
Sbjct: 107 GGFGKVYRALWRGEEVAVKAARLDPEKDPAVTAEQVCQEARLFGALQHPNIIALRGACLN 166
Query: 287 GPDLELQKLHYYIMEFAPNGSLNELLHEQMEVNYSLAQGLNWVHQCTKGVKYLHTYFDNP 466
P L L +ME+A G+L+ +L + + L +NW Q +G+ YLH P
Sbjct: 167 PPHLCL------VMEYARGGALSRVLAGRRVPPHVL---VNWAVQVARGMNYLHNDAPVP 217
Query: 467 ILHRDIKPANLLLFN-------SGTVVKLCDFGNVKSVQQTENTADSGTYVYMAPEVMNS 625
I+HRD+K N+L+ + TV+K+ DFG + +T + +GTY +MAPEV+
Sbjct: 218 IIHRDLKSINILILEAIENHNLADTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVI-- 275
Query: 626 VKDRKAYYNEKCDVYSIAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKERLDDIPGCPEK 805
R + +++ DV+S +++WE+L + P++ + ++ V + K L CPE
Sbjct: 276 ---RLSLFSKSSDVWSFGVLLWELLTGEVPYREI-DALAVAYGVAMNKLTLPIPSTCPEP 331
Query: 806 LQRLIKCCWKHQYKERPNISDVCEGL 883
RL++ CW RP+ + + L
Sbjct: 332 FARLLEECWDPDPHGRPDFGSILKRL 357
>sp|P80192|M3K9_HUMAN Mitogen-activated protein kinase kinase kinase 9 (Mixed lineage
kinase 1)
Length = 1104
Score = 110 bits (274), Expect = 7e-24
Identities = 76/263 (28%), Positives = 127/263 (48%), Gaps = 13/263 (4%)
Frame = +2
Query: 134 GSYGSVQRILLQNTECAVKYFR------VREHYQKNLAEVNSLSICDHANIVKLFAAGPD 295
G +G V R E AVK R + + + E ++ H NI+ L G
Sbjct: 153 GGFGKVYRAFWIGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIAL--RGVC 210
Query: 296 LELQKLHYYIMEFAPNGSLNELLHEQMEVNYSLAQGLNWVHQCTKGVKYLHTYFDNPILH 475
L+ L +MEFA G LN +L + L +NW Q +G+ YLH PI+H
Sbjct: 211 LKEPNL-CLVMEFARGGPLNRVLSGKRIPPDIL---VNWAVQIARGMNYLHDEAIVPIIH 266
Query: 476 RDIKPANLLLFN-------SGTVVKLCDFGNVKSVQQTENTADSGTYVYMAPEVMNSVKD 634
RD+K +N+L+ S ++K+ DFG + +T + +GTY +MAPEV+
Sbjct: 267 RDLKSSNILILQKVENGDLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVI----- 321
Query: 635 RKAYYNEKCDVYSIAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKERLDDIPGCPEKLQR 814
R + +++ DV+S +++WE+L + PF+ + ++ V + K L CPE +
Sbjct: 322 RASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGL-AVAYGVAMNKLALPIPSTCPEPFAK 380
Query: 815 LIKCCWKHQYKERPNISDVCEGL 883
L++ CW RP+ +++ + L
Sbjct: 381 LMEDCWNPDPHSRPSFTNILDQL 403
>sp|Q5TCX8|M3KL4_HUMAN Mitogen-activated protein kinase kinase kinase (Mixed lineage
kinase 4)
Length = 1036
Score = 107 bits (267), Expect = 4e-23
Identities = 79/276 (28%), Positives = 125/276 (45%), Gaps = 26/276 (9%)
Frame = +2
Query: 134 GSYGSVQRILLQNTECAVKYFRVREHYQKNLA------EVNSLSICDHANIVKLFAAGPD 295
G +G V R Q E AVK R A E ++ H NI++L
Sbjct: 133 GGFGQVYRATWQGQEVAVKAARQDPEQDAAAAAESVRREARLFAMLRHPNIIELRGVC-- 190
Query: 296 LELQKLHY-YIMEFAPNGSLNELLHEQMEVNYSLAQG------------LNWVHQCTKGV 436
LQ+ H ++EFA G+LN L A G +NW Q +G+
Sbjct: 191 --LQQPHLCLVLEFARGGALNRALAAANAAPDPRAPGPRRARRIPPHVLVNWAVQIARGM 248
Query: 437 KYLHTYFDNPILHRDIKPANLLLFNS-------GTVVKLCDFGNVKSVQQTENTADSGTY 595
YLH PILHRD+K +N+LL +K+ DFG + +T + +GTY
Sbjct: 249 LYLHEEAFVPILHRDLKSSNILLLEKIEHDDICNKTLKITDFGLAREWHRTTKMSTAGTY 308
Query: 596 VYMAPEVMNSVKDRKAYYNEKCDVYSIAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKER 775
+MAPEV+ S + +++ D++S +++WE+L + P++ + ++ V + K
Sbjct: 309 AWMAPEVIKS-----SLFSKGSDIWSYGVLLWELLTGEVPYRGIDGL-AVAYGVAVNKLT 362
Query: 776 LDDIPGCPEKLQRLIKCCWKHQYKERPNISDVCEGL 883
L CPE +L+K CW+ RP+ + + E L
Sbjct: 363 LPIPSTCPEPFAKLMKECWQQDPHIRPSFALILEQL 398
>sp|Q05609|CTR1_ARATH Serine/threonine-protein kinase CTR1
Length = 821
Score = 103 bits (257), Expect = 6e-22
Identities = 79/258 (30%), Positives = 129/258 (50%), Gaps = 10/258 (3%)
Frame = +2
Query: 134 GSYGSVQRILLQNTECAVKYFRVREHYQKN----LAEVNSLSICDHANIVKLFAA---GP 292
GS+G+V R ++ AVK ++ + + L EV + H NIV A P
Sbjct: 560 GSFGTVHRAEWHGSDVAVKILMEQDFHAERVNEFLREVAIMKRLRHPNIVLFMGAVTQPP 619
Query: 293 DLELQKLHYYIMEFAPNGSLNELLHEQ-MEVNYSLAQGLNWVHQCTKGVKYLHTYFDNPI 469
+L + + E+ GSL LLH+ + L+ + KG+ YLH + PI
Sbjct: 620 NLSI------VTEYLSRGSLYRLLHKSGAREQLDERRRLSMAYDVAKGMNYLHNR-NPPI 672
Query: 470 LHRDIKPANLLLFNSGTVVKLCDFG--NVKSVQQTENTADSGTYVYMAPEVMNSVKDRKA 643
+HRD+K NLL+ TV K+CDFG +K+ + + +GT +MAPEV+ R
Sbjct: 673 VHRDLKSPNLLVDKKYTV-KVCDFGLSRLKASTFLSSKSAAGTPEWMAPEVL-----RDE 726
Query: 644 YYNEKCDVYSIAIIIWEILVRQKPFKNAPNVFSIMNTVVLKKERLDDIPGCPEKLQRLIK 823
NEK DVYS +I+WE+ Q+P+ N N ++ V K +RL+ ++ +I+
Sbjct: 727 PSNEKSDVYSFGVILWELATLQQPWGNL-NPAQVVAAVGFKCKRLEIPRNLNPQVAAIIE 785
Query: 824 CCWKHQYKERPNISDVCE 877
CW ++ +RP+ + + +
Sbjct: 786 GCWTNEPWKRPSFATIMD 803
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,748,260
Number of Sequences: 369166
Number of extensions: 2127445
Number of successful extensions: 8219
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6592
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6887
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8838279920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)