Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_G11
(929 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P35520|CBS_HUMAN Cystathionine beta-synthase (Serine sul... 313 3e-85
sp|P32232|CBS_RAT Cystathionine beta-synthase (Serine sulfh... 296 4e-80
sp|P46794|CBS_DICDI Cystathionine beta-synthase (Serine sul... 238 2e-62
sp|P32582|CBS_YEAST Cystathionine beta-synthase (Serine sul... 178 2e-44
sp|Q9ZMW6|CYSM_HELPJ Cysteine synthase (O-acetylserine sulf... 84 6e-16
sp|P0A535|CYSK_MYCBO Cysteine synthase A (O-acetylserine su... 82 2e-15
sp|P56067|CYSM_HELPY Cysteine synthase (O-acetylserine sulf... 80 6e-15
sp|O32978|CYSK_MYCLE Cysteine synthase A (O-acetylserine su... 80 8e-15
sp|O23735|CYSK2_BRAJU Cysteine synthase (O-acetylserine sul... 73 1e-12
sp|P0ABK5|CYSK_ECOLI Cysteine synthase A (O-acetylserine su... 71 5e-12
>sp|P35520|CBS_HUMAN Cystathionine beta-synthase (Serine sulfhydrase) (Beta-thionase)
Length = 551
Score = 313 bits (803), Expect = 3e-85
Identities = 150/309 (48%), Positives = 212/309 (68%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPFGSILA 180
PLAH+D TA+EIL CD K+DM ARK+K KCP C++IGVDP GSILA
Sbjct: 229 PLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILA 288
Query: 181 QPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLLCGG 360
+P+ LN+T T YEVEGIGYDFIPTVLDRT VDKW+K D+E+F AR LI +EGLLCGG
Sbjct: 289 EPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGG 348
Query: 361 SSGTAVYATIQAIKDFNLPNTSRVIVILPDSVRNYMTKFLSDDWMYDRNFLDKPMTNQSN 540
S+G+ V ++A ++ L R +VILPDSVRNYMTKFLSD WM + FL + +
Sbjct: 349 SAGSTVAVAVKAAQE--LQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKK 406
Query: 541 DQQFERKISSLEMNAPMTVQPSVTVQQALELLKKEGFDQIPVVEANGCIVGVATVGLIMS 720
+ ++ L ++AP+TV P++T +E+L+++GFDQ PVV+ G I+G+ T+G ++S
Sbjct: 407 PWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVILGMVTLGNMLS 466
Query: 721 MLMKKSLNLTDPISKAIYKNFKKVNLNQTVQQLSRLLDIDHFVLVVHQQRQIGASGTEEV 900
L+ + +D + K IYK FK++ L T+ +LS +L++DHF LVVH+Q Q ++G
Sbjct: 467 SLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQ 526
Query: 901 KEMIYGIIT 927
++M++G++T
Sbjct: 527 RQMVFGVVT 535
>sp|P32232|CBS_RAT Cystathionine beta-synthase (Serine sulfhydrase) (Beta-thionase)
(Hemoprotein H-450)
Length = 561
Score = 296 bits (759), Expect = 4e-80
Identities = 150/323 (46%), Positives = 210/323 (65%), Gaps = 14/323 (4%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPFGSILA 180
PLAH+D TAEEIL CD K+DM ARK+K KCP CK+IGVDP GSILA
Sbjct: 226 PLAHYDDTAEEILQQCDGKVDMLVASAGTGGTITGIARKLKEKCPGCKIIGVDPEGSILA 285
Query: 181 QPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLLCGG 360
+P+ LN+T T YEVEGIGYDFIPTVLDR VD+W+K D +SF AR LI +EGLLCGG
Sbjct: 286 EPEELNQTEQTAYEVEGIGYDFIPTVLDRAVVDRWFKSNDDDSFAFARMLISQEGLLCGG 345
Query: 361 SSGTAVYATIQAIKDFNLPNTSRVIVILPDSVRNYMTKFLSDDWMYDRNFLDKPMTNQSN 540
SSG+A+ ++A ++ L R +VILPDSVRNYM+KFLSD WM + F+ K +
Sbjct: 346 SSGSAMAVAVKAAQE--LKEGQRCVVILPDSVRNYMSKFLSDKWMLQKGFM-KEELSVKR 402
Query: 541 DQQFERKISSLEMNAPMTVQPSVTVQQALELLKKEGFDQIPVVEANGCIVGVATVGLIMS 720
+ ++ L ++AP+TV P+VT + + +L+++GFDQ PVV +G I+G+ T+G ++S
Sbjct: 403 PWWWHLRVQELSLSAPLTVLPTVTCEHTIAILREKGFDQAPVVNESGAILGMVTLGNMLS 462
Query: 721 MLMKKSLNLTDPISKAIYKNFKKVNLNQTVQQLSRLLDIDHFVLVVHQQRQ--------- 873
L+ + +D + K +YK FK ++L T+ LS +L++DHF LVVH+Q Q
Sbjct: 463 SLLAGKVRPSDEVCKVLYKQFKPIHLTDTLGMLSHILEMDHFALVVHEQIQSRDQAWSGV 522
Query: 874 IGA-----SGTEEVKEMIYGIIT 927
+G +G + M++G++T
Sbjct: 523 VGGPTDRNNGVSSKQLMVFGVVT 545
>sp|P46794|CBS_DICDI Cystathionine beta-synthase (Serine sulfhydrase) (Beta-thionase)
Length = 497
Score = 238 bits (606), Expect = 2e-62
Identities = 136/302 (45%), Positives = 191/302 (63%), Gaps = 10/302 (3%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPFGSILA 180
PLAH+DGTAEE+L C+ KIDM ARKIK + PNC V+GVDP GSILA
Sbjct: 183 PLAHYDGTAEELLEQCEGKIDMIVCTAGTGGTITGIARKIKERLPNCIVVGVDPHGSILA 242
Query: 181 QPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLLCGG 360
QP+SLN TN + Y++EGIGYDFIP VL+R VD+W K DKESFIMARRLI+EEGLLC G
Sbjct: 243 QPESLNNTNKS-YKIEGIGYDFIPNVLERKLVDQWIKTDDKESFIMARRLIKEEGLLCAG 301
Query: 361 SSGTAVYATIQAIKDFNLPNTSRVIVILPDSVRNYMTKFLSDDWMYDRNFLDKPMTNQSN 540
SSG+A+ + A K L R +V+L DS+RNYMTK L+DDW+ D F+D ++
Sbjct: 302 SSGSAMVGALLAAK--QLKKGQRCVVLLADSIRNYMTKHLNDDWLVDNGFVDPEY--KTK 357
Query: 541 DQQFERK-----ISSLEMNAPMTVQPSVTVQQALELLKKEGFDQIPVVEANGCIVGVATV 705
DQQ E K + L + P+T+ + T A++LL++ GFDQ+PVV + ++ + +
Sbjct: 358 DQQEEEKYHGATVKDLTLPKPITISATTTCAAAVQLLQQYGFDQLPVVSESKKVLVNSLL 417
Query: 706 GLIMSMLMKKSLNLTDPISKAIY---KNFKKVNLNQ--TVQQLSRLLDIDHFVLVVHQQR 870
++ KK++ TD +SK ++ KN K + + Q ++ LS+ + +H +V +
Sbjct: 418 VTSLTYASKKAVP-TDAVSKVMFRFTKNEKYIPITQSTSLATLSKFFE-NHSSAIVTEND 475
Query: 871 QI 876
+I
Sbjct: 476 EI 477
>sp|P32582|CBS_YEAST Cystathionine beta-synthase (Serine sulfhydrase) (Beta-thionase)
Length = 507
Score = 178 bits (451), Expect = 2e-44
Identities = 99/283 (34%), Positives = 156/283 (55%), Gaps = 18/283 (6%)
Frame = +1
Query: 1 PLAHFDGTAEEIL-----IDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPF 165
P AH+ GT EI ++ + + ++ +K + +++G DPF
Sbjct: 164 PEAHYFGTGREIQRQLEDLNLFDNLRAVVAGAGTGGTISGISKYLKEQNDKIQIVGADPF 223
Query: 166 GSILAQPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEG 345
GSILAQP++LNKT++T Y+VEGIGYDF+P VLDR +D WYK DK SF AR+LI EG
Sbjct: 224 GSILAQPENLNKTDITDYKVEGIGYDFVPQVLDRKLIDVWYKTDDKPSFKYARQLISNEG 283
Query: 346 LLCGGSSGTAVYATIQAIKDF-NLPNTSRVIVILPDSVRNYMTKFLSDDWMYDRNFLDKP 522
+L GGSSG+A A ++ +D L ++ I PDS+R+Y+TKF+ D+W+ N D
Sbjct: 284 VLVGGSSGSAFTAVVKYCEDHPELTEDDVIVAIFPDSIRSYLTKFVDDEWLKKNNLWDDD 343
Query: 523 MTNQSNDQQFERK------------ISSLEMNAPMTVQPSVTVQQALELLKKEGFDQIPV 666
+ + + + E + L + ++V+ + V +++LK GFDQ+PV
Sbjct: 344 VLARFDSSKLEASTTKYADVFGNATVKDLHLKPVVSVKETAKVTDVIKILKDNGFDQLPV 403
Query: 667 VEANGCIVGVATVGLIMSMLMKKSLNLTDPISKAIYKNFKKVN 795
+ +G + G+ T+ ++ L + N D K Y +FKK+N
Sbjct: 404 LTEDGKLSGLVTLSELLRKLSINNSN-NDNTIKGKYLDFKKLN 445
>sp|Q9ZMW6|CYSM_HELPJ Cysteine synthase (O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) (CSase)
Length = 305
Score = 84.0 bits (206), Expect = 6e-16
Identities = 50/158 (31%), Positives = 80/158 (50%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPFGSILA 180
P A++ A EI+ + + A+ +K + PN ++IGV+P GSIL
Sbjct: 152 PAAYYHTLAPEIVQELGTNLTSFVAGIGSGGTFAGMAKYLKERIPNIRLIGVEPEGSIL- 210
Query: 181 QPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLLCGG 360
N +E+EGIG +FIP +D++ + D+E F R+L ++ GLL G
Sbjct: 211 -----NGGEPGPHEIEGIGVEFIPPFFANLDIDRFETISDEEGFSYTRKLAKKNGLLVGS 265
Query: 361 SSGTAVYATIQAIKDFNLPNTSRVIVILPDSVRNYMTK 474
SSG A A ++ ++ LP S+V+ I PD Y++K
Sbjct: 266 SSGAAFAAALKEVQ--RLPEGSQVLTIFPDMADRYLSK 301
>sp|P0A535|CYSK_MYCBO Cysteine synthase A (O-acetylserine sulfhydrylase A)
(O-acetylserine (Thiol)-lyase A) (CSase A)
sp|P0A534|CYSK_MYCTU Cysteine synthase A (O-acetylserine sulfhydrylase A)
(O-acetylserine (Thiol)-lyase A) (CSase A)
Length = 310
Score = 82.0 bits (201), Expect = 2e-15
Identities = 54/164 (32%), Positives = 83/164 (50%), Gaps = 1/164 (0%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPFGSILA 180
P H TAEE+ D D K+D+ A+ IK + P+ + + V+P S +
Sbjct: 151 PAIHRVTTAEEVWRDTDGKVDIVVAGVGTGGTITGVAQVIKERKPSARFVAVEPAASPVL 210
Query: 181 QPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLLCGG 360
+ ++GIG F+P VLD+ VD+ VG++++ +ARRL REEGLL G
Sbjct: 211 SGGQKGP-----HPIQGIGAGFVPPVLDQDLVDEIITVGNEDALNVARRLAREEGLLVGI 265
Query: 361 SSGTAVYATIQAIKDFNLPNTSR-VIVILPDSVRNYMTKFLSDD 489
SSG A A +Q + N + ++V+LPD Y++ L D
Sbjct: 266 SSGAATVAALQVAR--RPENAGKLIVVVLPDFGERYLSTPLFAD 307
>sp|P56067|CYSM_HELPY Cysteine synthase (O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) (CSase)
Length = 306
Score = 80.5 bits (197), Expect = 6e-15
Identities = 50/158 (31%), Positives = 78/158 (49%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPFGSILA 180
P A++ A EI+ + + AR +K + P ++IGV+P GSIL
Sbjct: 153 PAAYYHTLAPEIVQELGTNLTSFVAGIGSGGTFAGTARYLKERIPAIRLIGVEPEGSIL- 211
Query: 181 QPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLLCGG 360
N +E+EGIG +FIP + +D + + D+E F R+L ++ GLL G
Sbjct: 212 -----NGGEPGPHEIEGIGVEFIPPFFENLDIDGFETISDEEGFSYTRKLAKKNGLLVGS 266
Query: 361 SSGTAVYATIQAIKDFNLPNTSRVIVILPDSVRNYMTK 474
SSG A A ++ + LP S+V+ I PD Y++K
Sbjct: 267 SSGAAFVAALKEAQ--RLPEGSQVLTIFPDVADRYLSK 302
>sp|O32978|CYSK_MYCLE Cysteine synthase A (O-acetylserine sulfhydrylase A)
(O-acetylserine (Thiol)-lyase A) (CSase A)
Length = 310
Score = 80.1 bits (196), Expect = 8e-15
Identities = 54/164 (32%), Positives = 82/164 (50%), Gaps = 1/164 (0%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPFGSILA 180
P H TAEE+ D D K+D+ AR IK + P + + V+P S +
Sbjct: 151 PAIHAATTAEEVWRDTDGKVDIFVSGIGTGGTITGVARIIKERRPATQFVAVEPAASPVL 210
Query: 181 QPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLLCGG 360
+ ++GIG F+P+VL VD+ VG++E+ +ARRL +EEGLL G
Sbjct: 211 SGGQKGP-----HPIQGIGAGFVPSVLVLDLVDEIVTVGNEEALDLARRLAKEEGLLVGI 265
Query: 361 SSGTAVYATIQAIKDFNLPNTSR-VIVILPDSVRNYMTKFLSDD 489
S+G AV A + + NT + ++V+LPD Y++ L D
Sbjct: 266 SAGAAVCAALHVAR--RPENTGKLIVVVLPDFGERYLSTMLFAD 307
>sp|O23735|CYSK2_BRAJU Cysteine synthase (O-acetylserine sulfhydrylase) (O-acetylserine
(Thiol)-lyase) (CSase) (OAS-TL) (OAS-TL6)
Length = 324
Score = 72.8 bits (177), Expect = 1e-12
Identities = 52/163 (31%), Positives = 75/163 (46%), Gaps = 1/163 (0%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGVDPFGSILA 180
P H++ T EI + KID + +K + PN K+ GV+P S +
Sbjct: 156 PKIHYETTGPEIWKGTEGKIDGFISGIGTGGTITGAGKYLKEQNPNVKLYGVEPVESAIL 215
Query: 181 QPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLLCGG 360
++++GIG FIP VL+ +D+ +V ES MAR L REEGLL G
Sbjct: 216 SGGKPGP-----HKIQGIGAGFIPNVLETNLIDEVVQVSSDESIDMARLLAREEGLLVGI 270
Query: 361 SSGTAVYATIQAIKDFNLPNTSRVIV-ILPDSVRNYMTKFLSD 486
SSG A A I+ K N ++ V + P Y++ L D
Sbjct: 271 SSGAAAAAAIKLAK--RPENAGKLFVAVFPSFGERYLSTVLFD 311
>sp|P0ABK5|CYSK_ECOLI Cysteine synthase A (O-acetylserine sulfhydrylase A)
(O-acetylserine (Thiol)-lyase A) (CSase A) (Sulfate
starvation-induced protein 5) (SSI5)
sp|P0ABK6|CYSK_ECO57 Cysteine synthase A (O-acetylserine sulfhydrylase A)
(O-acetylserine (Thiol)-lyase A) (CSase A) (Sulfate
starvation-induced protein 5) (SSI5)
Length = 323
Score = 70.9 bits (172), Expect = 5e-12
Identities = 54/166 (32%), Positives = 82/166 (49%), Gaps = 3/166 (1%)
Frame = +1
Query: 1 PLAHFDGTAEEILIDCDNKIDMXXXXXXXXXXXXXXARKIKLKCPNCKVIGV--DPFGS- 171
P H T EI D D ++D+ +R IK +I V +P S
Sbjct: 150 PEIHEKTTGPEIWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSP 209
Query: 172 ILAQPDSLNKTNLTFYEVEGIGYDFIPTVLDRTYVDKWYKVGDKESFIMARRLIREEGLL 351
++AQ + + ++++GIG FIP LD VDK + ++E+ ARRL+ EEG+L
Sbjct: 210 VIAQALAGEEIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGIL 269
Query: 352 CGGSSGTAVYATIQAIKDFNLPNTSRVIVILPDSVRNYMTKFLSDD 489
G SSG AV A ++ +D + N ++VILP S Y++ L D
Sbjct: 270 AGISSGAAVAAALKLQEDESFTN-KNIVVILPSSGERYLSTALFAD 314
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,161,340
Number of Sequences: 369166
Number of extensions: 2071261
Number of successful extensions: 6184
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6152
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9558791870
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)