Planarian EST Database


Dr_sW_017_E16

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_017_E16
         (739 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9UEW8|STK39_HUMAN  STE20/SPS1-related proline-alanine-ri...    57   4e-08
sp|Q863I2|OXSR1_PIG  Serine/threonine-protein kinase OSR1 (O...    56   1e-07
sp|Q6P9R2|OXSR1_MOUSE  Serine/threonine-protein kinase OSR1 ...    56   1e-07
sp|O95747|OXSR1_HUMAN  Serine/threonine-protein kinase OSR1 ...    55   1e-07
sp|Q5R495|OXSR1_PONPY  Serine/threonine-protein kinase OSR1 ...    55   1e-07
sp|Q9Z1W9|STK39_MOUSE  STE20/SPS1-related proline-alanine-ri...    52   1e-06
sp|O88506|STK39_RAT  STE20/SPS1-related proline-alanine-rich...    51   3e-06
sp|P57994|MURC_MYCLE  UDP-N-acetylmuramate--L-alanine ligase...    32   1.8  
sp|Q44774|FTSA_BORBU  Cell division protein ftsA                   32   2.3  
sp|Q01222|VB18_VACCV  Ankyrin repeat protein B18                   30   6.7  
>sp|Q9UEW8|STK39_HUMAN STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20
           related kinase) (Serine/threonine-protein kinase 39)
           (DCHT)
          Length = 547

 Score = 57.4 bits (137), Expect = 4e-08
 Identities = 45/155 (29%), Positives = 74/155 (47%), Gaps = 18/155 (11%)
 Frame = +3

Query: 99  DEKQEKNPKDFSGEWNFDIKGKNXXXXXX----------------EDPPGPKTKTSTAEN 230
           DEK E+    FS E +  +K +N                      + PP        A +
Sbjct: 392 DEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASS 451

Query: 231 VFYRITLRKRNPKQNNELQDISFIYVLGKDSVDVLARELVEADLLDGCDLLLVAHNLSEL 410
               + LR RN ++  EL DI F +  G+D+ D +++EL  A L+DG D+++VA NL ++
Sbjct: 452 CAVNLVLRLRNSRK--ELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKI 509

Query: 411 VAEP-TMRERVFALNSP-PASGVINEADLNGYAKV 509
           V +P  ++   F L S    S + +E  L G+A++
Sbjct: 510 VDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQL 544
>sp|Q863I2|OXSR1_PIG Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
           protein)
          Length = 529

 Score = 55.8 bits (133), Expect = 1e-07
 Identities = 35/91 (38%), Positives = 58/91 (63%), Gaps = 2/91 (2%)
 Frame = +3

Query: 243 ITLRKRNPKQNNELQDISFIYVLGKDSVDVLARELVEADLLDGCDLLLVAHNLSELVAEP 422
           + LR RN K+  EL DI F +  G+D+ + +++EL+ A L+DG DL++VA NL ++V EP
Sbjct: 438 LVLRLRNSKK--ELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEP 495

Query: 423 -TMRERVFALNS-PPASGVINEADLNGYAKV 509
            + R   F L S    S + +++ L G+A++
Sbjct: 496 QSNRSVTFKLASGVEGSDIPDDSKLIGFAQL 526
>sp|Q6P9R2|OXSR1_MOUSE Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
           protein)
          Length = 527

 Score = 55.8 bits (133), Expect = 1e-07
 Identities = 40/117 (34%), Positives = 65/117 (55%), Gaps = 11/117 (9%)
 Frame = +3

Query: 192 PPGPKTKTSTAENVFYR---------ITLRKRNPKQNNELQDISFIYVLGKDSVDVLARE 344
           PP    K + A++   R         + LR RN K+  EL DI F +  G+D+ + +++E
Sbjct: 410 PPAEPAKPAQAQSSGERSQETKIPISLVLRLRNSKK--ELNDIRFEFTPGRDTAEGVSQE 467

Query: 345 LVEADLLDGCDLLLVAHNLSELVAEP-TMRERVFALNS-PPASGVINEADLNGYAKV 509
           L+ A L+DG DL++VA NL ++V EP + R   F L S    S + ++  L G+A++
Sbjct: 468 LISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQL 524
>sp|O95747|OXSR1_HUMAN Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
           protein)
          Length = 527

 Score = 55.5 bits (132), Expect = 1e-07
 Identities = 35/91 (38%), Positives = 57/91 (62%), Gaps = 2/91 (2%)
 Frame = +3

Query: 243 ITLRKRNPKQNNELQDISFIYVLGKDSVDVLARELVEADLLDGCDLLLVAHNLSELVAEP 422
           + LR RN K+  EL DI F +  G+D+ + +++EL+ A L+DG DL++VA NL ++V EP
Sbjct: 436 LVLRLRNSKK--ELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEP 493

Query: 423 -TMRERVFALNS-PPASGVINEADLNGYAKV 509
            + R   F L S    S + ++  L G+A++
Sbjct: 494 QSNRSVTFKLASGVEGSDIPDDGKLIGFAQL 524
>sp|Q5R495|OXSR1_PONPY Serine/threonine-protein kinase OSR1 (Oxidative stress-responsive 1
           protein)
          Length = 527

 Score = 55.5 bits (132), Expect = 1e-07
 Identities = 35/91 (38%), Positives = 57/91 (62%), Gaps = 2/91 (2%)
 Frame = +3

Query: 243 ITLRKRNPKQNNELQDISFIYVLGKDSVDVLARELVEADLLDGCDLLLVAHNLSELVAEP 422
           + LR RN K+  EL DI F +  G+D+ + +++EL+ A L+DG DL++VA NL ++V EP
Sbjct: 436 LVLRLRNSKK--ELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEP 493

Query: 423 -TMRERVFALNS-PPASGVINEADLNGYAKV 509
            + R   F L S    S + ++  L G+A++
Sbjct: 494 QSNRSVTFKLASGVEGSDIPDDGKLIGFAQL 524
>sp|Q9Z1W9|STK39_MOUSE STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20
           related kinase) (Serine/threonine-protein kinase 39)
          Length = 556

 Score = 52.4 bits (124), Expect = 1e-06
 Identities = 33/91 (36%), Positives = 56/91 (61%), Gaps = 2/91 (2%)
 Frame = +3

Query: 243 ITLRKRNPKQNNELQDISFIYVLGKDSVDVLARELVEADLLDGCDLLLVAHNLSELVAEP 422
           + LR RN ++  EL DI F +  G+D+ D +++EL  A L+DG D+++VA NL ++V +P
Sbjct: 465 LVLRLRNSRK--ELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDP 522

Query: 423 -TMRERVFALNSP-PASGVINEADLNGYAKV 509
             ++   F L S    S + +E  L G+A++
Sbjct: 523 KALKTLTFKLASGCDGSEIPDEVKLIGFAQL 553
>sp|O88506|STK39_RAT STE20/SPS1-related proline-alanine-rich protein kinase (Ste-20
           related kinase) (Serine/threonine-protein kinase 39)
           (Pancreatic serine/threonine-protein kinase) (PS/TK)
           (PSTK1)
          Length = 553

 Score = 51.2 bits (121), Expect = 3e-06
 Identities = 32/91 (35%), Positives = 56/91 (61%), Gaps = 2/91 (2%)
 Frame = +3

Query: 243 ITLRKRNPKQNNELQDISFIYVLGKDSVDVLARELVEADLLDGCDLLLVAHNLSELVAEP 422
           + LR RN ++  EL DI F +  G+D+ D +++EL  A L+DG D+++VA NL ++V +P
Sbjct: 462 LVLRLRNSRK--ELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDP 519

Query: 423 -TMRERVFALNSP-PASGVINEADLNGYAKV 509
             ++   F L S    + + +E  L G+A++
Sbjct: 520 KALKTLTFKLASGCDGAEIPDEVKLIGFAQL 550
>sp|P57994|MURC_MYCLE UDP-N-acetylmuramate--L-alanine ligase
           (UDP-N-acetylmuramoyl-L-alanine synthetase)
          Length = 495

 Score = 32.0 bits (71), Expect = 1.8
 Identities = 20/50 (40%), Positives = 26/50 (52%)
 Frame = +3

Query: 243 ITLRKRNPKQNNELQDISFIYVLGKDSVDVLARELVEADLLDGCDLLLVA 392
           IT+R   PK N EL +        +  + VL R  V A L+DGC  L+VA
Sbjct: 78  ITIRTAIPKTNPELVEAR------RRGIPVLLRSAVLARLMDGCTTLMVA 121
>sp|Q44774|FTSA_BORBU Cell division protein ftsA
          Length = 413

 Score = 31.6 bits (70), Expect = 2.3
 Identities = 22/86 (25%), Positives = 41/86 (47%), Gaps = 4/86 (4%)
 Frame = +3

Query: 321 SVDVLARELVEADLLDGCDLLLVAHNLSELVAEPTMRERVFALNSPPASGVINEAD---- 488
           ++D ++  +  A+L+ GCD+  ++ ++S    E T    V A+NS      INE D    
Sbjct: 53  ALDSISNSIEAAELISGCDITSLSVSMSGSSVEGTNSRGVVAINSKTRE--INEEDVERV 110

Query: 489 LNGYAKVLLRFAHEDLPVLPKT*FTD 566
           +     +++    E L V+P+    D
Sbjct: 111 IEAAKAIVIPMDREILHVIPQEFIVD 136
>sp|Q01222|VB18_VACCV Ankyrin repeat protein B18
          Length = 574

 Score = 30.0 bits (66), Expect = 6.7
 Identities = 22/54 (40%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
 Frame = +3

Query: 465 SGVINEADLNGYAKVLLRFAHED--LPVLPKT*FTDKNCLFSCLAFEISYLMKY 620
           S ++NE  LN Y K +      D   PV     FTDKNCL + L  EI Y + Y
Sbjct: 509 SNIMNERYLNVYYKDMYVSKVYDKLFPV-----FTDKNCLLTLLPSEIIYEILY 557
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,669,173
Number of Sequences: 369166
Number of extensions: 1292873
Number of successful extensions: 3299
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3298
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6631293200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)