Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_D22
(606 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28648|CD63_RAT CD63 antigen (AD1 antigen) 47 5e-05
sp|P41731|CD63_MOUSE CD63 antigen 44 2e-04
sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane... 42 0.001
sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane... 40 0.005
sp|Q9UKR8|TNS16_HUMAN Tetraspanin-16 (Tspan-16) (Transmembr... 40 0.006
sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18) 38 0.017
sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane... 38 0.017
sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET... 38 0.023
sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane... 38 0.023
sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated anti... 37 0.030
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
Length = 238
Score = 46.6 bits (109), Expect = 5e-05
Identities = 42/187 (22%), Positives = 70/187 (37%), Gaps = 4/187 (2%)
Frame = +2
Query: 5 CLLITY----SILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLTNVLEKDFNGILNTNAT 172
CL+IT+ S++ K++F K+ K + N L N AT
Sbjct: 81 CLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFSKSFQKQMQNYLTD------NKTAT 134
Query: 173 LFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDPNKK 352
+ K+ E KCCG +N TD++ + K P CC
Sbjct: 135 ILDKL----QKENKCCGASNYTDWERIPGM-------AKDRVPDSCCI------------ 171
Query: 353 FEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIV 532
N C D +STI+ GC+ ++ + KN+ E++ I+
Sbjct: 172 --------NI-TVGCGNDFKESTIHTQGCVETIAAWLRKNVLLVAGAALGIAFVEVLGII 222
Query: 533 FACCVMR 553
F+CC+++
Sbjct: 223 FSCCLVK 229
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 44.3 bits (103), Expect = 2e-04
Identities = 40/187 (21%), Positives = 71/187 (37%), Gaps = 4/187 (2%)
Frame = +2
Query: 5 CLLITY----SILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLTNVLEKDFNGILNTNAT 172
CL+IT+ S++ K++F K+ + + N L+ N AT
Sbjct: 81 CLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFNKSFQQQMQNYLKD------NKTAT 134
Query: 173 LFTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDPNKK 352
+ K+ E CCG +N TD++++ K P CC
Sbjct: 135 ILDKL----QKENNCCGASNYTDWENIPGM-------AKDRVPDSCCI------------ 171
Query: 353 FEYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIV 532
N C D +STI+ GC+ ++ + KN+ E++ I+
Sbjct: 172 --------NI-TVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFVEVLGII 222
Query: 533 FACCVMR 553
F+CC+++
Sbjct: 223 FSCCLVK 229
>sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
(T245 protein) (Tetraspanin TM4-D) (A15 homolog)
Length = 245
Score = 42.0 bits (97), Expect = 0.001
Identities = 39/156 (25%), Positives = 58/156 (37%)
Frame = +2
Query: 86 KNKFQKNLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWK 265
KN F+ N K+L K +N + + KI L CCGV TD+ R+W
Sbjct: 119 KNSFKNNYEKAL-----KQYNSTGDYRSHAVDKIQNT----LHCCGV---TDY---RDWT 163
Query: 266 STSSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIP 445
T+ K +P CCKL++ C D +N GC
Sbjct: 164 DTNYYSEKG-FPKSCCKLED-----------------------CTPQRDADKVNNEGCFI 199
Query: 446 SLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 553
++ +IE M F+++ I A C+ R
Sbjct: 200 KVMTIIESEMGVVAGISFGVACFQLIGIFLAYCLSR 235
>sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
Length = 245
Score = 40.0 bits (92), Expect = 0.005
Identities = 35/156 (22%), Positives = 53/156 (33%)
Frame = +2
Query: 86 KNKFQKNLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWK 265
KN F+ N +L K++N + + KI + L CCGV N D+ +
Sbjct: 119 KNSFKSNYENAL-----KEYNSTGDYRSEAVDKIQST----LHCCGVTNYGDWKGTNYYS 169
Query: 266 STSSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIP 445
T +P CCKL + C D +N GC
Sbjct: 170 ETG-------FPKSCCKL-----------------------EGCYPQRDADKVNEEGCFI 199
Query: 446 SLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 553
++ IE M F+++ I A C+ R
Sbjct: 200 KVMTTIESEMGVVAGISFGVACFQLIGIFLAYCLSR 235
>sp|Q9UKR8|TNS16_HUMAN Tetraspanin-16 (Tspan-16) (Transmembrane 4 superfamily member 16)
(Tetraspanin TM4-B)
Length = 245
Score = 39.7 bits (91), Expect = 0.006
Identities = 19/40 (47%), Positives = 22/40 (55%)
Frame = +2
Query: 197 IMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCK 316
+M +LKCCGVNN TDF S+ T YP CCK
Sbjct: 147 VMEKLKCCGVNNYTDFSG-----SSFEMTTGHTYPRSCCK 181
>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
Length = 248
Score = 38.1 bits (87), Expect = 0.017
Identities = 34/154 (22%), Positives = 66/154 (42%), Gaps = 1/154 (0%)
Frame = +2
Query: 95 FQKNLGKSL-TNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKST 271
F+++L + T L K + G +T+ +F+ + ++M CCGVN DF ++
Sbjct: 108 FREHLTREFFTKELTKHYQGDNDTD--VFSATWNSVMITFGCCGVNGPEDFKLASVFRLL 165
Query: 272 SSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFENDNCPFDNDKSTININGCIPSL 451
+ T+++ P CC+ +P + +++ + PF IN GC +
Sbjct: 166 TLD-TEEV-PKACCR------REPQTRDGVVLSREECQLGRNPF------INKQGCYTVI 211
Query: 452 IDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 553
++ E + E+ +VFA C+ R
Sbjct: 212 LNTFETYVYLAGAFAIGVLAIELFLMVFAMCLFR 245
>sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A)
Length = 253
Score = 38.1 bits (87), Expect = 0.017
Identities = 24/105 (22%), Positives = 47/105 (44%)
Frame = +2
Query: 2 KCLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLTNVLEKDFNGILNTNATLFT 181
+C L T+ I+ + K + + +S+ V K +NG TN +
Sbjct: 80 RCGLATFVIILLLVFVTEVVVVVLGYVYRAKVENEVDRSIQKVY-KTYNG---TNPDAAS 135
Query: 182 KIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCK 316
+ + +L CCG++N +D+++ +K T + Q P+ CC+
Sbjct: 136 RAIDYVQRQLHCCGIHNYSDWENTDWFKETKN----QSVPLSCCR 176
>sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET-1) (Tetraspanin TM4-C)
Length = 241
Score = 37.7 bits (86), Expect = 0.023
Identities = 27/99 (27%), Positives = 37/99 (37%)
Frame = +2
Query: 176 FTKIFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDPNKKF 355
FT+++ M LKCCG N TDF+ +K S+ +P CC
Sbjct: 136 FTQVWNTTMKGLKCCGFTNYTDFEDSPYFKENSA------FPPFCC-------------- 175
Query: 356 EYNDAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKN 472
ND N N+ C + GC L+ I N
Sbjct: 176 --NDNVTNTANETCTKQKAHDQ-KVEGCFNQLLYDIRTN 211
>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
(Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
Length = 253
Score = 37.7 bits (86), Expect = 0.023
Identities = 20/75 (26%), Positives = 40/75 (53%)
Frame = +2
Query: 92 KFQKNLGKSLTNVLEKDFNGILNTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWKST 271
K + + +S+ V K +NG TN+ ++ + +L CCG++N +D+++ +K T
Sbjct: 110 KVENEVDRSIQKVY-KTYNG---TNSDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKET 165
Query: 272 SSSGTKQIYPVGCCK 316
+ Q P+ CC+
Sbjct: 166 KN----QSVPLSCCR 176
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
(Lysosome-associated membrane glycoprotein 3) (LAMP-3)
(Ocular melanoma-associated antigen) (OMA81H)
(Granulophysin) (Tetraspanin-30) (Tspan-30)
Length = 238
Score = 37.4 bits (85), Expect = 0.030
Identities = 35/183 (19%), Positives = 61/183 (33%)
Frame = +2
Query: 5 CLLITYSILXXXXXXXXXXXXXXXXXEKNKFQKNLGKSLTNVLEKDFNGILNTNATLFTK 184
CL+IT++I ++K + +E N T
Sbjct: 81 CLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMEN----YPKNNHT--AS 134
Query: 185 IFTAIMFELKCCGVNNVTDFDSVRNWKSTSSSGTKQIYPVGCCKLKEKYQYDPNKKFEYN 364
I + + KCCG N TD++ + S +K P CC
Sbjct: 135 ILDRMQADFKCCGAANYTDWEKI-------PSMSKNRVPDSCCINVTV------------ 175
Query: 365 DAKKNFENDNCPFDNDKSTININGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACC 544
C + ++ I+ GC+ + + KN+ E++ IVFACC
Sbjct: 176 ---------GCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACC 226
Query: 545 VMR 553
+++
Sbjct: 227 LVK 229
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,411,185
Number of Sequences: 369166
Number of extensions: 1258646
Number of successful extensions: 3131
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3043
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3122
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4699949280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)