Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_D16
(892 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O13433|PLC1_CANAL 1-phosphatidylinositol-4,5-bisphosphat... 33 0.82
sp|P93398|GBB2_TOBAC Guanine nucleotide-binding protein bet... 33 0.82
sp|Q9PHL7|ENGB_CAMJE Probable GTP-binding protein engB 33 1.1
sp|P93339|GBB_NICPL Guanine nucleotide-binding protein beta... 33 1.1
sp|P93397|GBB1_TOBAC Guanine nucleotide-binding protein bet... 33 1.4
sp|Q40507|GBB3_TOBAC Guanine nucleotide-binding protein bet... 32 2.4
sp|O42937|COPB2_SCHPO Probable coatomer beta' subunit (Beta... 31 5.3
sp|P47455|SCPA_MYCGE Segregation and condensation protein A 30 6.9
sp|Q89AA4|CYOB_BUCBP Ubiquinol oxidase polypeptide I (Cytoc... 30 6.9
sp|Q01477|UBP3_YEAST Ubiquitin carboxyl-terminal hydrolase ... 30 9.0
>sp|O13433|PLC1_CANAL 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase 1
(Phosphoinositide phospholipase C) (PLC-1)
(Phospholipase C-1)
Length = 1099
Score = 33.5 bits (75), Expect = 0.82
Identities = 25/105 (23%), Positives = 48/105 (45%), Gaps = 4/105 (3%)
Frame = +1
Query: 52 FINYENSPFKNRSNISLSMKRLWFSLI----KKKQLQPLFVQHFLFSKHYQTEKDANQND 219
+I E S ++ NIS +++ W ++I KK L+ L H T+ ND
Sbjct: 302 YIQNEGSGYREELNISQKLEKNWITIIYFNHKKNSLKSL---------HLITD-----ND 347
Query: 220 SLFNSFNSEIKNIRIIHKEHENLFSMAINQIDNSLLVTSTSKEII 354
F S I N++ + + F + +N++D + + +KE++
Sbjct: 348 HDFKKLISAIYNLKQLRSQLAKEFLIDLNELDENYVKMLLNKELL 392
>sp|P93398|GBB2_TOBAC Guanine nucleotide-binding protein beta subunit 2
Length = 377
Score = 33.5 bits (75), Expect = 0.82
Identities = 17/72 (23%), Positives = 38/72 (52%)
Frame = +1
Query: 577 IHGIQIIKSHPLLPYYITGNTNGQVGVFEWSVDAAVVKTYSPIQKGKITSLNFDANGNKF 756
+ + I S+P L +++G+ + +++ V + +T+ +G + ++ F +GN+F
Sbjct: 207 VQSVSISSSNPRL--FVSGSCDSTARLWDTRVASRAQRTFYG-HEGDVNTVKFFPDGNRF 263
Query: 757 GMGDSEGNFSLF 792
G G +G LF
Sbjct: 264 GTGSDDGTCRLF 275
>sp|Q9PHL7|ENGB_CAMJE Probable GTP-binding protein engB
Length = 198
Score = 33.1 bits (74), Expect = 1.1
Identities = 25/104 (24%), Positives = 51/104 (49%), Gaps = 7/104 (6%)
Frame = +1
Query: 55 INYENSPFKNRSNISLSMKRLWFS----LIKKKQLQPLFVQHFLFSKHYQTEKDANQNDS 222
IN+ + P + +S ++K +W +K + LF+ H + S+H E D N ND
Sbjct: 77 INFIDLPGFGYAKVSKNLKEIWNQNLDEFLKLRTSIKLFI-HLIDSRHTHLEIDVNLNDY 135
Query: 223 LFNSFNSEIKNIRII---HKEHENLFSMAINQIDNSLLVTSTSK 345
L + K +++ K +++ + N+ +S+LV++ +K
Sbjct: 136 LKRFLRPDQKILKVFTKCDKLNQSEKAKLKNEFKDSILVSNLNK 179
>sp|P93339|GBB_NICPL Guanine nucleotide-binding protein beta subunit (NpGPB1)
Length = 377
Score = 33.1 bits (74), Expect = 1.1
Identities = 17/72 (23%), Positives = 38/72 (52%)
Frame = +1
Query: 577 IHGIQIIKSHPLLPYYITGNTNGQVGVFEWSVDAAVVKTYSPIQKGKITSLNFDANGNKF 756
+ + I S+P L +++G+ + +++ V + +T+ +G + ++ F +GN+F
Sbjct: 207 VQSVSISSSNPRL--FVSGSCDTTARLWDTRVASRAQRTFY-CHEGDVNTVKFFPDGNRF 263
Query: 757 GMGDSEGNFSLF 792
G G +G LF
Sbjct: 264 GTGSEDGTCRLF 275
>sp|P93397|GBB1_TOBAC Guanine nucleotide-binding protein beta subunit 1
Length = 377
Score = 32.7 bits (73), Expect = 1.4
Identities = 17/72 (23%), Positives = 38/72 (52%)
Frame = +1
Query: 577 IHGIQIIKSHPLLPYYITGNTNGQVGVFEWSVDAAVVKTYSPIQKGKITSLNFDANGNKF 756
+ + I S+P L +++G+ + +++ V + +T+ +G + ++ F +GN+F
Sbjct: 207 VQSVSISSSNPRL--FVSGSCDTTARLWDTRVASRAQRTFYG-HEGDVNTVKFFPDGNRF 263
Query: 757 GMGDSEGNFSLF 792
G G +G LF
Sbjct: 264 GTGSEDGTCRLF 275
>sp|Q40507|GBB3_TOBAC Guanine nucleotide-binding protein beta subunit
Length = 375
Score = 32.0 bits (71), Expect = 2.4
Identities = 17/72 (23%), Positives = 38/72 (52%)
Frame = +1
Query: 577 IHGIQIIKSHPLLPYYITGNTNGQVGVFEWSVDAAVVKTYSPIQKGKITSLNFDANGNKF 756
+ + I S+P L +++G+ + +++ V + +T+ +G + ++ F +GN+F
Sbjct: 207 VQSVSISSSNPRL--FVSGSCDTTARLWDNRVASRAQRTFYG-HEGDVNTVKFFPDGNRF 263
Query: 757 GMGDSEGNFSLF 792
G G +G LF
Sbjct: 264 GTGSEDGTCRLF 275
>sp|O42937|COPB2_SCHPO Probable coatomer beta' subunit (Beta'-coat protein) (Beta'-COP)
Length = 796
Score = 30.8 bits (68), Expect = 5.3
Identities = 17/72 (23%), Positives = 33/72 (45%)
Frame = +1
Query: 586 IQIIKSHPLLPYYITGNTNGQVGVFEWSVDAAVVKTYSPIQKGKITSLNFDANGNKFGMG 765
++ + HP P+ I + NGQVG+ W+ + + I I + F A N F G
Sbjct: 17 VKAVDFHPTEPWVIASHYNGQVGI--WNYNTQTLVRSFDINDVPIRACAFIARKNWFVCG 74
Query: 766 DSEGNFSLFHFS 801
+ +++++
Sbjct: 75 SDDFQVRVYNYN 86
>sp|P47455|SCPA_MYCGE Segregation and condensation protein A
Length = 471
Score = 30.4 bits (67), Expect = 6.9
Identities = 22/79 (27%), Positives = 39/79 (49%), Gaps = 1/79 (1%)
Frame = +1
Query: 136 KKQLQPLFVQHFLFSKHYQTEKDANQNDSLFNSFNS-EIKNIRIIHKEHENLFSMAINQI 312
K QL+P + +FS + QTE N ++FN++ + N +I L + N+I
Sbjct: 44 KAQLKPFKLA--VFSDNLQTELTPNSKLTVFNNYQQLLVDNNDLIIFATPTLVQLFDNEI 101
Query: 313 DNSLLVTSTSKEIIEISVN 369
D +++ TSK + + N
Sbjct: 102 DQLIVINPTSKSKDQFNCN 120
>sp|Q89AA4|CYOB_BUCBP Ubiquinol oxidase polypeptide I (Cytochrome o subunit 1) (Oxidase
BO(3) subunit 1) (Cytochrome o ubiquinol oxidase subunit
1)
Length = 659
Score = 30.4 bits (67), Expect = 6.9
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +3
Query: 600 ISSIVTLLYNWKYKWSSWC 656
IS I+T+ W+Y W+ WC
Sbjct: 31 ISIIITIAQKWQYLWNEWC 49
>sp|Q01477|UBP3_YEAST Ubiquitin carboxyl-terminal hydrolase 3 (Ubiquitin thiolesterase 3)
(Ubiquitin-specific processing protease 3)
(Deubiquitinating enzyme 3)
Length = 912
Score = 30.0 bits (66), Expect = 9.0
Identities = 23/72 (31%), Positives = 32/72 (44%)
Frame = +1
Query: 46 KNFINYENSPFKNRSNISLSMKRLWFSLIKKKQLQPLFVQHFLFSKHYQTEKDANQNDSL 225
K F YE PFK+ S + K+ F K Q L +Q FS KD +
Sbjct: 745 KKFSEYELLPFKSSSGNDVEAKKQTFI---DKLPQVLLIQFKRFSFINNVNKDNAMTN-- 799
Query: 226 FNSFNSEIKNIR 261
+N++N I+ IR
Sbjct: 800 YNAYNGRIEKIR 811
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,148,576
Number of Sequences: 369166
Number of extensions: 1515711
Number of successful extensions: 4124
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4008
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4122
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8934348180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)