Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_B03
(476 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated anti... 41 0.001
sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane... 40 0.002
sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane... 39 0.005
sp|P28648|CD63_RAT CD63 antigen (AD1 antigen) 39 0.006
sp|Q9UKR8|TNS16_HUMAN Tetraspanin-16 (Tspan-16) (Transmembr... 39 0.006
sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18) 37 0.023
sp|Q5RC27|TSN1_PONPY Tetraspanin-1 (Tspan-1) 37 0.023
sp|P41731|CD63_MOUSE CD63 antigen 37 0.031
sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET... 35 0.068
sp|Q7YQL0|TSN7_PANTR Tetraspanin-7 (Tspan-7) (Transmembrane... 34 0.20
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
(Lysosome-associated membrane glycoprotein 3) (LAMP-3)
(Ocular melanoma-associated antigen) (OMA81H)
(Granulophysin) (Tetraspanin-30) (Tspan-30)
Length = 238
Score = 41.2 bits (95), Expect = 0.001
Identities = 28/123 (22%), Positives = 46/123 (37%)
Frame = +1
Query: 28 IFTAIMFELKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQYDPNKKFEYN 207
I + + KCCG N TD W+ PS ++ P CC
Sbjct: 135 ILDRMQADFKCCGAANYTD------WEKIPSMSKNRV-PDSCCINVTV------------ 175
Query: 208 DAKKNFENDNCPFNNDKSKINENGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACC 387
C N ++ I++ GC+ + + KN+ E++ IVFACC
Sbjct: 176 ---------GCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACC 226
Query: 388 VMR 396
+++
Sbjct: 227 LVK 229
>sp|O70401|TSN6_MOUSE Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
Length = 245
Score = 40.4 bits (93), Expect = 0.002
Identities = 29/119 (24%), Positives = 40/119 (33%)
Frame = +1
Query: 40 IMFELKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKK 219
I L CCGV N D+ + T +P CCKL
Sbjct: 147 IQSTLHCCGVTNYGDWKGTNYYSETG-------FPKSCCKL------------------- 180
Query: 220 NFENDNCPFNNDKSKINENGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 396
+ C D K+NE GC ++ IE M F+++ I A C+ R
Sbjct: 181 ----EGCYPQRDADKVNEEGCFIKVMTTIESEMGVVAGISFGVACFQLIGIFLAYCLSR 235
>sp|O43657|TSN6_HUMAN Tetraspanin-6 (Tspan-6) (Transmembrane 4 superfamily member 6)
(T245 protein) (Tetraspanin TM4-D) (A15 homolog)
Length = 245
Score = 39.3 bits (90), Expect = 0.005
Identities = 30/115 (26%), Positives = 43/115 (37%)
Frame = +1
Query: 52 LKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFEN 231
L CCGV TD+ R+W T K +P CCKL++
Sbjct: 151 LHCCGV---TDY---RDWTDTNYYSEKG-FPKSCCKLED--------------------- 182
Query: 232 DNCPFNNDKSKINENGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 396
C D K+N GC ++ +IE M F+++ I A C+ R
Sbjct: 183 --CTPQRDADKVNNEGCFIKVMTIIESEMGVVAGISFGVACFQLIGIFLAYCLSR 235
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
Length = 238
Score = 38.9 bits (89), Expect = 0.006
Identities = 31/134 (23%), Positives = 51/134 (38%), Gaps = 2/134 (1%)
Frame = +1
Query: 1 NTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQY 180
N AT+ K+ E KCCG +N TD W+ P ++ P CC
Sbjct: 130 NKTATILDKL----QKENKCCGASNYTD------WERIPGMAKDRV-PDSCCI------- 171
Query: 181 DPNKKFEYNDAKKNFENDNCPFNND--KSKINENGCIPSLIDLIEKNMXXXXXXXXXXXX 354
N ND +S I+ GC+ ++ + KN+
Sbjct: 172 ----------------NITVGCGNDFKESTIHTQGCVETIAAWLRKNVLLVAGAALGIAF 215
Query: 355 FEIMCIVFACCVMR 396
E++ I+F+CC+++
Sbjct: 216 VEVLGIIFSCCLVK 229
>sp|Q9UKR8|TNS16_HUMAN Tetraspanin-16 (Tspan-16) (Transmembrane 4 superfamily member 16)
(Tetraspanin TM4-B)
Length = 245
Score = 38.9 bits (89), Expect = 0.006
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +1
Query: 40 IMFELKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCK 159
+M +LKCCGVNN TDF S +++ T T YP CCK
Sbjct: 147 VMEKLKCCGVNNYTDF-SGSSFEMT----TGHTYPRSCCK 181
>sp|Q80WR1|TSN18_MOUSE Tetraspanin-18 (Tspan-18)
Length = 248
Score = 37.0 bits (84), Expect = 0.023
Identities = 27/132 (20%), Positives = 55/132 (41%)
Frame = +1
Query: 1 NTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQY 180
+ + +F+ + ++M CCGVN DF ++ + T+++ P CC+
Sbjct: 128 DNDTDVFSATWNSVMITFGCCGVNGPEDFKLASVFRLL-TLDTEEV-PKACCR------R 179
Query: 181 DPNKKFEYNDAKKNFENDNCPFNNDKSKINENGCIPSLIDLIEKNMXXXXXXXXXXXXFE 360
+P + +++ + PF IN+ GC +++ E + E
Sbjct: 180 EPQTRDGVVLSREECQLGRNPF------INKQGCYTVILNTFETYVYLAGAFAIGVLAIE 233
Query: 361 IMCIVFACCVMR 396
+ +VFA C+ R
Sbjct: 234 LFLMVFAMCLFR 245
>sp|Q5RC27|TSN1_PONPY Tetraspanin-1 (Tspan-1)
Length = 241
Score = 37.0 bits (84), Expect = 0.023
Identities = 28/99 (28%), Positives = 37/99 (37%)
Frame = +1
Query: 19 FTKIFTAIMFELKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQYDPNKKF 198
FT+++ M LKCCG N TDF+ +P +P CC
Sbjct: 136 FTQVWNTTMKGLKCCGFTNYTDFED------SPYLKENHAFPPFCC-------------- 175
Query: 199 EYNDAKKNFENDNCPFNNDKSKINENGCIPSLIDLIEKN 315
ND N ND C + + E GC L+ I N
Sbjct: 176 --NDNITNTANDTCTKQKAEDQKVE-GCFNQLLYDIRTN 211
>sp|P41731|CD63_MOUSE CD63 antigen
Length = 238
Score = 36.6 bits (83), Expect = 0.031
Identities = 30/134 (22%), Positives = 50/134 (37%), Gaps = 2/134 (1%)
Frame = +1
Query: 1 NTNATLFTKIFTAIMFELKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQY 180
N AT+ K+ E CCG +N TD W+ P ++ P CC
Sbjct: 130 NKTATILDKL----QKENNCCGASNYTD------WENIPGMAKDRV-PDSCCI------- 171
Query: 181 DPNKKFEYNDAKKNFENDNCPFNND--KSKINENGCIPSLIDLIEKNMXXXXXXXXXXXX 354
N ND +S I+ GC+ ++ + KN+
Sbjct: 172 ----------------NITVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAF 215
Query: 355 FEIMCIVFACCVMR 396
E++ I+F+CC+++
Sbjct: 216 VEVLGIIFSCCLVK 229
>sp|O60635|TSN1_HUMAN Tetraspanin-1 (Tspan-1) (Tetraspan NET-1) (Tetraspanin TM4-C)
Length = 241
Score = 35.4 bits (80), Expect = 0.068
Identities = 28/102 (27%), Positives = 38/102 (37%), Gaps = 3/102 (2%)
Frame = +1
Query: 19 FTKIFTAIMFELKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQYDPNKKF 198
FT+++ M LKCCG N TDF+ +P +P CC
Sbjct: 136 FTQVWNTTMKGLKCCGFTNYTDFED------SPYFKENSAFPPFCC-------------- 175
Query: 199 EYNDAKKNFENDNCPFNNDKSKINE---NGCIPSLIDLIEKN 315
ND N N+ C K K ++ GC L+ I N
Sbjct: 176 --NDNVTNTANETC----TKQKAHDQKVEGCFNQLLYDIRTN 211
>sp|Q7YQL0|TSN7_PANTR Tetraspanin-7 (Tspan-7) (Transmembrane 4 superfamily member 2)
Length = 244
Score = 33.9 bits (76), Expect = 0.20
Identities = 28/115 (24%), Positives = 43/115 (37%)
Frame = +1
Query: 52 LKCCGVNNVTDFDSVRNWQFTPSSGTKQIYPVGCCKLKEKYQYDPNKKFEYNDAKKNFEN 231
L CCGV N T NW +P I P CC + D N + +N
Sbjct: 143 LSCCGVQNYT------NWSTSPYFLEHGI-PPSCCM----NETDCNPQDLHN-------- 183
Query: 232 DNCPFNNDKSKINENGCIPSLIDLIEKNMXXXXXXXXXXXXFEIMCIVFACCVMR 396
+K+N+ GC + +E NM +++ ++ ACC+ R
Sbjct: 184 ----LTVAATKVNQKGCYDLVTSFMETNMGIIAGVAFGIAFSQLIGMLLACCLSR 234
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,035,277
Number of Sequences: 369166
Number of extensions: 955149
Number of successful extensions: 2427
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2341
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2408
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2832714465
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)