Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_A08
(922 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O35453|HEPS_MOUSE Serine protease hepsin [Contains: Seri... 185 2e-46
sp|Q8K1T0|TMPS3_MOUSE Transmembrane protease, serine 3 181 2e-45
sp|P05981|HEPS_HUMAN Serine protease hepsin (Transmembrane ... 181 3e-45
sp|P57727|TMPS3_HUMAN Transmembrane protease, serine 3 (Ser... 181 3e-45
sp|Q6BEA2|PRS27_RAT Protease serine 27 precursor (Marapsin)... 180 6e-45
sp|Q9JIQ8|TMPS2_MOUSE Transmembrane protease, serine 2 (Epi... 177 3e-44
sp|Q05511|HEPS_RAT Serine protease hepsin [Contains: Serine... 177 4e-44
sp|Q8IU80|TMPS6_HUMAN Transmembrane protease, serine 6 (Mat... 177 5e-44
sp|P03952|KLKB1_HUMAN Plasma kallikrein precursor (Plasma p... 176 6e-44
sp|Q8BJR6|PRS27_MOUSE Protease serine 27 precursor (Marapsi... 176 8e-44
>sp|O35453|HEPS_MOUSE Serine protease hepsin [Contains: Serine protease hepsin
non-catalytic chain; Serine protease hepsin catalytic
chain]
Length = 436
Score = 185 bits (469), Expect = 2e-46
Identities = 102/254 (40%), Positives = 146/254 (57%), Gaps = 7/254 (2%)
Frame = +3
Query: 42 CGISNGSKYKVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFFFQDLP 221
CG ++ GG S G WPWQV + DG H CGG+L+ +WVLTAAHC F ++
Sbjct: 172 CGRRKLPVDRIVGGQDSSLGRWPWQVSLRYDGTHLCGGSLLSGDWVLTAAHC-FPERNRV 230
Query: 222 ISTWRIRAGKTQTNITEPPEQNIKVKKLIDHPGF------TLFTKYHDIALIQLAEPMYF 383
+S WR+ AG T P + V+ +I H G+ T+ +DIAL+ L+ +
Sbjct: 231 LSRWRVFAGAVAR--TSPHAVQLGVQAVIYHGGYLPFRDPTIDENSNDIALVHLSSSLPL 288
Query: 384 DYKFVAPVCLPNPGESF-DDHVCTLSGYGKTETSGEGASTYLLEVNIPIISADTCQEKHC 560
+++ PVCLP G++ D VCT++G+G T+ G+ A L E +PIIS + C
Sbjct: 289 T-EYIQPVCLPAAGQALVDGKVCTVTGWGNTQFYGQQAMV-LQEARVPIISNEVCNSPDF 346
Query: 561 NSNTIYEENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDT 740
N I + CAG+ EG DAC GDSGGP VCE S++G ++ L G+VS+G+GC
Sbjct: 347 YGNQIKPKMFCAGYPEGGIDACQGDSGGPFVCED-SISGTSRWRLCGIVSWGTGCALARK 405
Query: 741 PGVYTRITSYLDWI 782
PGVYT++T + +WI
Sbjct: 406 PGVYTKVTDFREWI 419
>sp|Q8K1T0|TMPS3_MOUSE Transmembrane protease, serine 3
Length = 453
Score = 181 bits (460), Expect = 2e-45
Identities = 99/253 (39%), Positives = 147/253 (58%), Gaps = 2/253 (0%)
Frame = +3
Query: 30 RSNKCGISNGSKYKVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFFF 209
+ + CG G ++ GG S +WPWQV ++ G H CGG++I P W++TAAHC +
Sbjct: 203 KCSACGTRTGYSPRIVGGNMSSLTQWPWQVSLQFQGYHLCGGSVITPLWIVTAAHCVY-- 260
Query: 210 QDL-PISTWRIRAGKTQTNITEPPEQNIKVKKLIDHPGFTLFTKYHDIALIQLAEPMYFD 386
DL +W ++ G ++ + P + V+K+I H + +DIAL++L+EP+ FD
Sbjct: 261 -DLYHPKSWTVQVG--LVSLMDSPVPSHLVEKIIYHSKYKPKRLGNDIALMKLSEPLTFD 317
Query: 387 YKFVAPVCLPNPGESF-DDHVCTLSGYGKTETSGEGASTYLLEVNIPIISADTCQEKHCN 563
+ + P+CLPN E+F D +C SG+G TE G+ AS L +P+IS C +
Sbjct: 318 -ETIQPICLPNSEENFPDGKLCWTSGWGATEDGGD-ASPVLNHAAVPLISNKICNHRDVY 375
Query: 564 SNTIYEENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDTP 743
I LCAG+ +G D+C GDSGGP+VC++ + + L G S+G GC + P
Sbjct: 376 GGIISPSMLCAGYLKGGVDSCQGDSGGPLVCQER-----RLWKLVGATSFGIGCAEVNKP 430
Query: 744 GVYTRITSYLDWI 782
GVYTRITS+LDWI
Sbjct: 431 GVYTRITSFLDWI 443
>sp|P05981|HEPS_HUMAN Serine protease hepsin (Transmembrane protease, serine 1)
[Contains: Serine protease hepsin non-catalytic chain;
Serine protease hepsin catalytic chain]
Length = 417
Score = 181 bits (459), Expect = 3e-45
Identities = 99/254 (38%), Positives = 145/254 (57%), Gaps = 7/254 (2%)
Frame = +3
Query: 42 CGISNGSKYKVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFFFQDLP 221
CG ++ GG + G WPWQV + DGAH CGG+L+ +WVLTAAHC F ++
Sbjct: 153 CGRRKLPVDRIVGGRDTSLGRWPWQVSLRYDGAHLCGGSLLSGDWVLTAAHC-FPERNRV 211
Query: 222 ISTWRIRAGKTQTNITEPPEQNIKVKKLIDHPGFTLF------TKYHDIALIQLAEPMYF 383
+S WR+ AG P + V+ ++ H G+ F +DIAL+ L+ P+
Sbjct: 212 LSRWRVFAGAVAQ--ASPHGLQLGVQAVVYHGGYLPFRDPNSEENSNDIALVHLSSPLPL 269
Query: 384 DYKFVAPVCLPNPGESF-DDHVCTLSGYGKTETSGEGASTYLLEVNIPIISADTCQEKHC 560
+++ PVCLP G++ D +CT++G+G T+ G+ A L E +PIIS D C
Sbjct: 270 T-EYIQPVCLPAAGQALVDGKICTVTGWGNTQYYGQQAGV-LQEARVPIISNDVCNGADF 327
Query: 561 NSNTIYEENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDT 740
N I + CAG+ EG DAC GDSGGP VCE S++ ++ L G+VS+G+GC
Sbjct: 328 YGNQIKPKMFCAGYPEGGIDACQGDSGGPFVCED-SISRTPRWRLCGIVSWGTGCALAQK 386
Query: 741 PGVYTRITSYLDWI 782
PGVYT+++ + +WI
Sbjct: 387 PGVYTKVSDFREWI 400
>sp|P57727|TMPS3_HUMAN Transmembrane protease, serine 3 (Serine protease TADG-12) (Tumor
associated differentially-expressed gene 12 protein)
Length = 454
Score = 181 bits (459), Expect = 3e-45
Identities = 98/248 (39%), Positives = 141/248 (56%), Gaps = 1/248 (0%)
Frame = +3
Query: 42 CGISNGSKYKVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFFFQDLP 221
CG G ++ GG S+ +WPWQ ++ G H CGG++I P W++TAAHC + LP
Sbjct: 207 CGHRRGYSSRIVGGNMSLLSQWPWQASLQFQGYHLCGGSVITPLWIITAAHCVYDLY-LP 265
Query: 222 ISTWRIRAGKTQTNITEPPEQNIKVKKLIDHPGFTLFTKYHDIALIQLAEPMYFDYKFVA 401
S W I+ G ++ + P + V+K++ H + +DIAL++LA P+ F+ + +
Sbjct: 266 KS-WTIQVGLV--SLLDNPAPSHLVEKIVYHSKYKPKRLGNDIALMKLAGPLTFN-EMIQ 321
Query: 402 PVCLPNPGESFDD-HVCTLSGYGKTETSGEGASTYLLEVNIPIISADTCQEKHCNSNTIY 578
PVCLPN E+F D VC SG+G TE AS L +P+IS C + I
Sbjct: 322 PVCLPNSEENFPDGKVCWTSGWGATEDGAGDASPVLNHAAVPLISNKICNHRDVYGGIIS 381
Query: 579 EENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDTPGVYTR 758
LCAG+ G D+C GDSGGP+VC++ + + L G S+G GC + PGVYTR
Sbjct: 382 PSMLCAGYLTGGVDSCQGDSGGPLVCQER-----RLWKLVGATSFGIGCAEVNKPGVYTR 436
Query: 759 ITSYLDWI 782
+TS+LDWI
Sbjct: 437 VTSFLDWI 444
>sp|Q6BEA2|PRS27_RAT Protease serine 27 precursor (Marapsin) (Pancreasin)
Length = 328
Score = 180 bits (456), Expect = 6e-45
Identities = 96/247 (38%), Positives = 140/247 (56%), Gaps = 9/247 (3%)
Frame = +3
Query: 69 KVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFFFQDLPISTWRIRAG 248
++ GG ++ GEWPWQV I+R+GAH CGG+LI P WVLTAAHC F IS +++ G
Sbjct: 37 RMVGGEDALEGEWPWQVSIQRNGAHFCGGSLIAPTWVLTAAHC--FSNTSDISIYQVLLG 94
Query: 249 KTQTNITEPPEQNIKVKKLIDHPGFTLFTKYHDIALIQLAEPMYFDYKFVAPVCLPNPGE 428
+ P + VK++ HP + D+AL++L P+ F K++ PVCLP+P
Sbjct: 95 ALKLQQPGPHALYVPVKRVKSHPEYQGMASSADVALVELQVPVTFT-KYILPVCLPDPSV 153
Query: 429 SFDDHV-CTLSGYGKTETSGEGASTYLLE-VNIPIISADTCQ-------EKHCNSNTIYE 581
F + C ++G+G + +L+ + +P+I C E TI +
Sbjct: 154 VFKSGMNCWVTGWGSPSEQDRLPNPRILQKLAVPLIDTPKCNLLYSKDAEADIQLKTIKD 213
Query: 582 ENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDTPGVYTRI 761
+ LCAGF EGK+DAC GDSGGP+VC + +V AGV+S+G GC ++ PGVY R+
Sbjct: 214 DMLCAGFAEGKKDACKGDSGGPLVCLV-----DQSWVQAGVISWGEGCARRNRPGVYIRV 268
Query: 762 TSYLDWI 782
S+ WI
Sbjct: 269 ASHYQWI 275
>sp|Q9JIQ8|TMPS2_MOUSE Transmembrane protease, serine 2 (Epitheliasin) (Plasmic
transmembrane protein X) [Contains: Transmembrane
protease, serine 2 non-catalytic chain; Transmembrane
protease, serine 2 catalytic chain]
Length = 490
Score = 177 bits (450), Expect = 3e-44
Identities = 101/256 (39%), Positives = 145/256 (56%), Gaps = 5/256 (1%)
Frame = +3
Query: 30 RSNKCGISNGSKY-KVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFF 206
R +CG+ + + ++ GG + PG+WPWQV + G H CGG++I P W++TAAHC
Sbjct: 239 RCIECGVRSVKRQSRIVGGLNASPGDWPWQVSLHVQGVHVCGGSIITPEWIVTAAHCV-- 296
Query: 207 FQDLPIS---TWRIRAGKTQTNITEPPEQNIKVKKLIDHPGFTLFTKYHDIALIQLAEPM 377
+ P+S W AG + ++ ++ +V+K+I HP + TK +DIAL++L P+
Sbjct: 297 --EEPLSGPRYWTAFAGILRQSLMFYGSRH-QVEKVISHPNYDSKTKNNDIALMKLQTPL 353
Query: 378 YFDYKFVAPVCLPNPGESFD-DHVCTLSGYGKTETSGEGASTYLLEVNIPIISADTCQEK 554
F+ V PVCLPNPG D D C +SG+G T G+ S L +P+I C K
Sbjct: 354 AFN-DLVKPVCLPNPGMMLDLDQECWISGWGATYEKGK-TSDVLNAAMVPLIEPSKCNSK 411
Query: 555 HCNSNTIYEENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGK 734
+ +N I +CAGF +G D+C GDSGGP+V K NG + L G S+GSGC
Sbjct: 412 YIYNNLITPAMICAGFLQGSVDSCQGDSGGPLVTLK---NGI--WWLIGDTSWGSGCAKA 466
Query: 735 DTPGVYTRITSYLDWI 782
PGVY +T + DWI
Sbjct: 467 LRPGVYGNVTVFTDWI 482
>sp|Q05511|HEPS_RAT Serine protease hepsin [Contains: Serine protease hepsin
non-catalytic chain; Serine protease hepsin catalytic
chain]
Length = 416
Score = 177 bits (449), Expect = 4e-44
Identities = 99/254 (38%), Positives = 143/254 (56%), Gaps = 7/254 (2%)
Frame = +3
Query: 42 CGISNGSKYKVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFFFQDLP 221
CG ++ GG S G WPWQV + DG H CGG+L+ +WVLTAAHC F ++
Sbjct: 152 CGRRKLPVDRIVGGQDSSLGRWPWQVSLRYDGTHLCGGSLLSGDWVLTAAHC-FPERNRV 210
Query: 222 ISTWRIRAGKTQTNITEPPEQNIKVKKLIDHPGF------TLFTKYHDIALIQLAEPMYF 383
+S WR+ AG T P + V+ +I H G+ T+ +DIAL+ L+ +
Sbjct: 211 LSRWRVFAGAVAR--TSPHAVQLGVQAVIYHGGYLPFRDPTIDENSNDIALVHLSSSLPL 268
Query: 384 DYKFVAPVCLPNPGESF-DDHVCTLSGYGKTETSGEGASTYLLEVNIPIISADTCQEKHC 560
+++ PVCLP G++ D VCT++G+G T+ G+ A L E +PIIS + C
Sbjct: 269 T-EYIQPVCLPAAGQALVDGKVCTVTGWGNTQFYGQQA-VVLQEARVPIISNEVCNSPDF 326
Query: 561 NSNTIYEENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDT 740
N I + CAG+ EG DAC GDSGG VCE ++G ++ L G+VS+G+GC
Sbjct: 327 YGNQIKPKMFCAGYPEGGIDACQGDSGGHFVCED-RISGTSRWRLCGIVSWGTGCALARK 385
Query: 741 PGVYTRITSYLDWI 782
PGVYT++ + +WI
Sbjct: 386 PGVYTKVIDFREWI 399
>sp|Q8IU80|TMPS6_HUMAN Transmembrane protease, serine 6 (Matriptase-2)
Length = 802
Score = 177 bits (448), Expect = 5e-44
Identities = 104/251 (41%), Positives = 142/251 (56%), Gaps = 3/251 (1%)
Frame = +3
Query: 42 CGISNGSKYKVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFFFQDLP 221
CG+ G ++ GG S GEWPWQ ++ G H CGGALI WV+TAAHC F +D
Sbjct: 559 CGLQ-GPSSRIVGGAVSSEGEWPWQASLQVRGRHICGGALIADRWVITAAHC--FQEDSM 615
Query: 222 IST--WRIRAGKTQTNITEPPEQNIKVKKLIDHPGFTLFTKYHDIALIQLAEPMYFDYKF 395
ST W + GK N P E + KV +L+ HP + +D+AL+QL P+
Sbjct: 616 ASTVLWTVFLGKVWQNSRWPGEVSFKVSRLLLHPYHEEDSHDYDVALLQLDHPVVRS-AA 674
Query: 396 VAPVCLPNPGESFDDHV-CTLSGYGKTETSGEGASTYLLEVNIPIISADTCQEKHCNSNT 572
V PVCLP F+ + C ++G+G G S L +V++ +I D C E +
Sbjct: 675 VRPVCLPARSHFFEPGLHCWITGWGALREGGP-ISNALQKVDVQLIPQDLCSEVY--RYQ 731
Query: 573 IYEENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDTPGVY 752
+ LCAG+++GK+DAC GDSGGP+VC+ LS F LAG+VS+G GCG + GVY
Sbjct: 732 VTPRMLCAGYRKGKKDACQGDSGGPLVCKALSGRWF----LAGLVSWGLGCGRPNYFGVY 787
Query: 753 TRITSYLDWIE 785
TRIT + WI+
Sbjct: 788 TRITGVISWIQ 798
>sp|P03952|KLKB1_HUMAN Plasma kallikrein precursor (Plasma prekallikrein) (Kininogenin)
(Fletcher factor) [Contains: Plasma kallikrein heavy
chain; Plasma kallikrein light chain]
Length = 638
Score = 176 bits (447), Expect = 6e-44
Identities = 96/247 (38%), Positives = 147/247 (59%), Gaps = 5/247 (2%)
Frame = +3
Query: 69 KVFGGWRSMPGEWPWQVQIE---RDGAHHCGGALIHPNWVLTAAHCTFFFQDLPIS-TWR 236
++ GG S GEWPWQV ++ H CGG+LI WVLTAAHC F LP+ WR
Sbjct: 390 RIVGGTNSSWGEWPWQVSLQVKLTAQRHLCGGSLIGHQWVLTAAHC---FDGLPLQDVWR 446
Query: 237 IRAGKTQTNITEPPEQNIKVKKLIDHPGFTLFTKYHDIALIQLAEPMYFDYKFVAPVCLP 416
I +G + ++K++I H + + HDIALI+L P+ + +F P+CLP
Sbjct: 447 IYSGILNLSDITKDTPFSQIKEIIIHQNYKVSEGNHDIALIKLQAPLNYT-EFQKPICLP 505
Query: 417 NPGESFDDHV-CTLSGYGKTETSGEGASTYLLEVNIPIISADTCQEKHCNSNTIYEENLC 593
+ G++ + C ++G+G ++ GE L +VNIP+++ + CQ+++ I + +C
Sbjct: 506 SKGDTSTIYTNCWVTGWGFSKEKGE-IQNILQKVNIPLVTNEECQKRY-QDYKITQRMVC 563
Query: 594 AGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDTPGVYTRITSYL 773
AG+KEG +DAC GDSGGP+VC+ NG + L G+ S+G GC ++ PGVYT++ Y+
Sbjct: 564 AGYKEGGKDACKGDSGGPLVCKH---NG--MWRLVGITSWGEGCARREQPGVYTKVAEYM 618
Query: 774 DWIEYET 794
DWI +T
Sbjct: 619 DWILEKT 625
>sp|Q8BJR6|PRS27_MOUSE Protease serine 27 precursor (Marapsin) (Pancreasin)
Length = 328
Score = 176 bits (446), Expect = 8e-44
Identities = 95/247 (38%), Positives = 140/247 (56%), Gaps = 9/247 (3%)
Frame = +3
Query: 69 KVFGGWRSMPGEWPWQVQIERDGAHHCGGALIHPNWVLTAAHCTFFFQDLPISTWRIRAG 248
++ GG ++ GEWPWQV I+R+G H CGG+LI P WVLTAAHC F IS +++ G
Sbjct: 37 RMVGGENALEGEWPWQVSIQRNGIHFCGGSLIAPTWVLTAAHC--FSNTSDISIYQVLLG 94
Query: 249 KTQTNITEPPEQNIKVKKLIDHPGFTLFTKYHDIALIQLAEPMYFDYKFVAPVCLPNPGE 428
+ P + VK++ +P + D+AL++L P+ F ++ PVCLP+P
Sbjct: 95 ALKLQQPGPHALYVPVKQVKSNPQYQGMASSADVALVELQGPVTFT-NYILPVCLPDPSV 153
Query: 429 SFDDHV-CTLSGYGKTETSGEGASTYLLE-VNIPIISADTCQ-------EKHCNSNTIYE 581
F+ + C ++G+G + +L+ + +PII C E TI +
Sbjct: 154 IFESGMNCWVTGWGSPSEQDRLPNPRVLQKLAVPIIDTPKCNLLYNKDVESDFQLKTIKD 213
Query: 582 ENLCAGFKEGKRDACTGDSGGPIVCEKLSVNGFKQFVLAGVVSYGSGCGGKDTPGVYTRI 761
+ LCAGF EGK+DAC GDSGGP+VC + +V AGV+S+G GC ++ PGVY R+
Sbjct: 214 DMLCAGFAEGKKDACKGDSGGPLVCLV-----DQSWVQAGVISWGEGCARRNRPGVYIRV 268
Query: 762 TSYLDWI 782
TS+ WI
Sbjct: 269 TSHHKWI 275
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,020,348
Number of Sequences: 369166
Number of extensions: 2243666
Number of successful extensions: 8221
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6386
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6850
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9414689480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)