Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_A07
(857 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 132 9e-31
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 131 2e-30
sp|P27636|CDC15_YEAST Cell division control protein 15 126 7e-29
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 126 9e-29
sp|P41892|CDC7_SCHPO Cell division control protein 7 120 6e-27
sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinas... 119 1e-26
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 119 1e-26
sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinas... 118 2e-26
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 117 5e-26
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 116 9e-26
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 132 bits (333), Expect = 9e-31
Identities = 94/276 (34%), Positives = 142/276 (51%), Gaps = 1/276 (0%)
Frame = +3
Query: 33 KDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMAN 212
K +++ + + +++L E + + L HP I++ G R I L+++
Sbjct: 98 KQVLIAANFASKEKTQAHIQELEEEVKLLKNLS-HPNIVRYLGTVREDDTLNILLEFVPG 156
Query: 213 GSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF 392
GS+ S L K G E + +++Q+L L++LH+ I+HRD+KG IL+DN IKL F
Sbjct: 157 GSISSLLEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADF 216
Query: 393 MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKA 572
++K + ++ T A+S GT W APEVI G+ SADIWS G ++E++TGKA
Sbjct: 217 GASKQVAELATMT----GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKA 272
Query: 573 PLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 749
P AI+ +GT K SDA K F+ CL P RPT +L+KH F+
Sbjct: 273 PWSQQYKEVAAIFFIGTTKSHPPIPDTLSSDA-KDFLLKCLQEVPNLRPTASELLKHPFV 331
Query: 750 NSAMEAD*FT*RVDIFSWIN*NNTYMLPK*INNCSS 857
+ T D+ S +N N + LP INN S
Sbjct: 332 MGKHKESAST---DLGSVLN-NLSTPLPLQINNTKS 363
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 131 bits (330), Expect = 2e-30
Identities = 89/276 (32%), Positives = 142/276 (51%), Gaps = 1/276 (0%)
Frame = +3
Query: 33 KDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMAN 212
K +++ + +++L E + + L HP I++ G R + I L+++
Sbjct: 97 KQVLITSNCASKEKTQAHIQELEEEVKLLKNLS-HPNIVRYLGTVREDETLNILLEFVPG 155
Query: 213 GSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF 392
GS+ S L K GA E + ++ Q+L L++LH+ I+HRD+KG IL+DN IKL F
Sbjct: 156 GSISSLLEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADF 215
Query: 393 MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKA 572
++K + ++ + A+S GT W APEVI G+ SADIWS G ++E++TGKA
Sbjct: 216 GASKQVAELATIS----GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKA 271
Query: 573 PLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 749
P AI+ +GT K P S F+ CL EP RPT +L+KH F+
Sbjct: 272 PWSQQYKEIAAIFHIGTTKS-HPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFV 330
Query: 750 NSAMEAD*FT*RVDIFSWIN*NNTYMLPK*INNCSS 857
+ + D+ S+++ N+ LP + N +S
Sbjct: 331 TGKQKE---SASKDLTSFMD-NSCSPLPSELTNITS 362
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 126 bits (317), Expect = 7e-29
Identities = 82/251 (32%), Positives = 121/251 (48%), Gaps = 1/251 (0%)
Frame = +3
Query: 6 VYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKAS 185
VY+ + +V +K+ ND EEL +M E S+ + L +H I+K +GF R
Sbjct: 39 VYKAINKHTDQVVAIKEVVYEND--EELNDIMAEISLLKNL-NHNNIVKYHGFIRKSYEL 95
Query: 186 YIKLDYMANGSLRSWLHKNGAQ-KEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLD 362
YI L+Y ANGSLR + ++ E + Q L L +LH + ++HRD+K ILL
Sbjct: 96 YILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANILLS 155
Query: 363 NNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGA 542
+ +KL F T N A + GT NW APE++ G + +DIWS GA
Sbjct: 156 ADNTVKLADF---------GVSTIVNSSALTLAGTLNWMAPEILGNRGASTLSDIWSLGA 206
Query: 543 VMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTV 722
+VE++T P L Y + + + P S+ LK F+ C RPT
Sbjct: 207 TVVEMLTKNPPYHNLTDANIYYAV---ENDTYYPPSSFSEPLKDFLSKCFVKNMYKRPTA 263
Query: 723 KDLMKHEFLNS 755
L+KH ++NS
Sbjct: 264 DQLLKHVWINS 274
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 126 bits (316), Expect = 9e-29
Identities = 79/240 (32%), Positives = 127/240 (52%), Gaps = 1/240 (0%)
Frame = +3
Query: 33 KDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMAN 212
K +++ + +++L E + + L HP I++ G R + I ++++
Sbjct: 97 KQVLIAPSSASKEKTQGHIRELEEEVQLLKNLS-HPNIVRYLGTVRESDSLNILMEFVPG 155
Query: 213 GSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGF 392
GS+ S L K G+ EP II ++KQ+L L++LH+ I+HRD+KG IL+DN I+L F
Sbjct: 156 GSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADF 215
Query: 393 MSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKA 572
++K + ++ + A+S GT W APEVI G+ SADIWS G ++E+ TGK
Sbjct: 216 GASKKVVELATV----NGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKP 271
Query: 573 PLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 749
P F A+ +G + K P S K F+ CL EP R + +L++H F+
Sbjct: 272 PWSEQYQQFAAVLHIG-RTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV 330
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 120 bits (300), Expect = 6e-27
Identities = 82/263 (31%), Positives = 129/263 (49%)
Frame = +3
Query: 3 SVYRCSKFGSKDMVVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKA 182
+VYR + + V +KK K +L + +E + + L DHP I+K G +++ +
Sbjct: 22 AVYRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLLKNL-DHPNIVKYRGSYQTNDS 80
Query: 183 SYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLD 362
I L+Y NGSLRS G E + ++ Q+L+ L +LH+Q ++HRD+KG IL
Sbjct: 81 LCIILEYCENGSLRSICKNFGKIPENLVALYTFQVLQGLLYLHNQGVIHRDIKGANILTT 140
Query: 363 NNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGA 542
+ IKL F A +H S G+ W APEVI G +++DIWS G
Sbjct: 141 KDGTIKLADFGVA-----TKINALEDH---SVVGSPYWMAPEVIELVGATTASDIWSVGC 192
Query: 543 VMVELITGKAPLFFLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTV 722
++EL+ G P + L A++R+ K P S A KSF+ C +P R
Sbjct: 193 TVIELLDGNPPYYDLDPTSALFRM--VKDEHPPLPSNISSAAKSFLMQCFQKDPNLRIKT 250
Query: 723 KDLMKHEFLNSAMEAD*FT*RVD 791
+ L+KH ++ + F+ +D
Sbjct: 251 RKLLKHPWVIMNQTSSKFSDAID 273
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1)
Length = 1607
Score = 119 bits (298), Expect = 1e-26
Identities = 89/255 (34%), Positives = 140/255 (54%), Gaps = 7/255 (2%)
Frame = +3
Query: 6 VYRCSKFGSKDMVVLKKYK-NPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKA 182
VY C + +++ +K+ + PND + +K+ E I+E + HP +++ G +
Sbjct: 1356 VYTCISVDTGELMAMKEIRFQPNDH-KTIKETADELKIFEGI-KHPNLVRYFGVELHREE 1413
Query: 183 SYIKLDYMANGSLRSWLHKNGAQKEPSIIR-FSKQILEALDFLHSQNILHRDLKGPKILL 359
YI ++Y G+L + + G Q+ +IR +SKQI A++ LH I+HRD+KG I L
Sbjct: 1414 MYIFMEYCDEGTLEE-VSRLGLQEH--VIRLYSKQITIAINVLHEHGIVHRDIKGANIFL 1470
Query: 360 DNNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN---GEGYGSSADI 527
++ IKL F S K+ +A +T V S GTA + APEVI GEG+G +ADI
Sbjct: 1471 TSSGLIKLGDFGCSVKLKNNA--QTMPGEV-NSTLGTAAYMAPEVITRAKGEGHGRAADI 1527
Query: 528 WSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEP 704
WS G V++E++TGK P + +FQ +Y++G K P + S K F+ CL+ +P
Sbjct: 1528 WSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPI--PERLSPEGKDFLSHCLESDP 1585
Query: 705 LNRPTVKDLMKHEFL 749
R T L+ H F+
Sbjct: 1586 KMRWTASQLLDHSFV 1600
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 119 bits (297), Expect = 1e-26
Identities = 79/259 (30%), Positives = 129/259 (49%), Gaps = 11/259 (4%)
Frame = +3
Query: 3 SVYRCSKFGSKDMVVLKKYKNPNDKDEE------LKKLMVEFSIYEKLGDHPRILKCNGF 164
+VY C + +M+ +K+ + P + ++ L E S + L DH I++ GF
Sbjct: 1188 AVYLCLNVTTGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKDL-DHLNIVQYLGF 1246
Query: 165 FRSVKASYIKLDYMANGSLRSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKG 344
+ L+Y+A GS+ S + G EP I + Q+L+ L +LHS+ ILHRD+K
Sbjct: 1247 ENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTTQVLKGLAYLHSKGILHRDMKA 1306
Query: 345 PKILLDNNLDIKLCGFMSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN-GEGYGSSA 521
+LLD + K+ F ++ D S + + GT W APE+++ +GY +
Sbjct: 1307 DNLLLDQDGICKISDFGISRKSKDIYSNS-----DMTMRGTVFWMAPEMVDTKQGYSAKV 1361
Query: 522 DIWSFGAVMVELITGKAPLFFLASFQAIYRLGTQKKI----EFAYPLKCSDALKSFVDPC 689
DIWS G +++E+ GK P L A++++G K E PL S ++F+D C
Sbjct: 1362 DIWSLGCIVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPL-ISQIGRNFLDAC 1420
Query: 690 LDPEPLNRPTVKDLMKHEF 746
+ P RPT +L+ H F
Sbjct: 1421 FEINPEKRPTANELLSHPF 1439
>sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4)
Length = 1597
Score = 118 bits (295), Expect = 2e-26
Identities = 88/255 (34%), Positives = 141/255 (55%), Gaps = 7/255 (2%)
Frame = +3
Query: 6 VYRCSKFGSKDMVVLKKYK-NPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKA 182
VY C + +++ +K+ + PND + +K+ E I+E + HP +++ G +
Sbjct: 1346 VYTCISVDTGELMAMKEIRFQPNDH-KTIKETADELKIFEGI-KHPNLVRYFGVELHREE 1403
Query: 183 SYIKLDYMANGSLRSWLHKNGAQKEPSIIR-FSKQILEALDFLHSQNILHRDLKGPKILL 359
YI ++Y G+L + + G Q+ +IR ++KQI A++ LH I+HRD+KG I L
Sbjct: 1404 MYIFMEYCDEGTLEE-VSRLGLQEH--VIRLYTKQITVAINVLHEHGIVHRDIKGANIFL 1460
Query: 360 DNNLDIKLCGF-MSAKMFPDASSETFRNHVAESNHGTANWTAPEVIN---GEGYGSSADI 527
++ IKL F S K+ +A +T V S GTA + APEVI GEG+G +ADI
Sbjct: 1461 TSSGLIKLGDFGCSVKLKNNA--QTMPGEV-NSTLGTAAYMAPEVITRAKGEGHGRAADI 1517
Query: 528 WSFGAVMVELITGKAPLF-FLASFQAIYRLGTQKKIEFAYPLKCSDALKSFVDPCLDPEP 704
WS G V++E++TGK P + +FQ +Y++G K P + S K+F+ CL+ +P
Sbjct: 1518 WSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPI--PERLSPEGKAFLSHCLESDP 1575
Query: 705 LNRPTVKDLMKHEFL 749
R T L+ H F+
Sbjct: 1576 KIRWTASQLLDHAFV 1590
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 117 bits (292), Expect = 5e-26
Identities = 77/238 (32%), Positives = 118/238 (49%), Gaps = 2/238 (0%)
Frame = +3
Query: 42 VVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMANGSL 221
VV K + + ++E++ + E ++ + D P + K G + +I ++Y+ GS
Sbjct: 61 VVAIKIIDLEEAEDEIEDIQQEITVLSQC-DSPYVTKYYGSYLKDTKLWIIMEYLGGGSA 119
Query: 222 RSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSA 401
L G E I ++IL+ LD+LHS+ +HRD+K +LL + ++KL F A
Sbjct: 120 LDLLEP-GPLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVA 178
Query: 402 KMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF 581
D + RN GT W APEVI Y S ADIWS G +EL G+ P
Sbjct: 179 GQLTDTQIK--RNTFV----GTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPPHS 232
Query: 582 FLASFQAIYRL--GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 749
L + ++ + +E Y S LK FV+ CL+ EP RPT K+L+KH+F+
Sbjct: 233 ELHPMKVLFLIPKNNPPTLEGNY----SKPLKEFVEACLNKEPSFRPTAKELLKHKFI 286
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 116 bits (290), Expect = 9e-26
Identities = 77/238 (32%), Positives = 118/238 (49%), Gaps = 2/238 (0%)
Frame = +3
Query: 42 VVLKKYKNPNDKDEELKKLMVEFSIYEKLGDHPRILKCNGFFRSVKASYIKLDYMANGSL 221
VV K + + ++E++ + E ++ + D P + K G + +I ++Y+ GS
Sbjct: 49 VVAIKIIDLEEAEDEIEDIQQEITVLSQC-DSPYVTKYYGSYLKDTKLWIIMEYLGGGSA 107
Query: 222 RSWLHKNGAQKEPSIIRFSKQILEALDFLHSQNILHRDLKGPKILLDNNLDIKLCGFMSA 401
L G E I ++IL+ LD+LHS+ +HRD+K +LL + ++KL F A
Sbjct: 108 LDLLEP-GPLDEIQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVA 166
Query: 402 KMFPDASSETFRNHVAESNHGTANWTAPEVINGEGYGSSADIWSFGAVMVELITGKAPLF 581
D + RN GT W APEVI Y S ADIWS G +EL G+ P
Sbjct: 167 GQLTDTQIK--RNTFV----GTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGEPPHS 220
Query: 582 FLASFQAIYRL--GTQKKIEFAYPLKCSDALKSFVDPCLDPEPLNRPTVKDLMKHEFL 749
L + ++ + +E Y S LK FV+ CL+ EP RPT K+L+KH+F+
Sbjct: 221 ELHPMKVLFLIPKNNPPTLEGNY----SKPLKEFVEACLNKEPSFRPTAKELLKHKFI 274
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,517,973
Number of Sequences: 369166
Number of extensions: 2195329
Number of successful extensions: 8946
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6899
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7333
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)