Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_P22-1
(484 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13503|MED21_HUMAN Mediator of RNA polymerase II transcr... 53 4e-07
sp|Q13201|MMRN1_HUMAN Multimerin-1 precursor (Endothelial c... 36 0.041
sp|P47822|MED21_YEAST RNA polymerase II mediator complex su... 34 0.15
sp|Q8JFQ6|K1C13_ONCMY Keratin, type I cytoskeletal 13 (Cyto... 32 1.00
sp|P40813|T1M_SALTY Type I restriction enzyme StySJI M prot... 31 1.7
sp|Q9P2G1|AKIB1_HUMAN Ankyrin repeat and IBR domain contain... 31 1.7
sp|Q96CV9|OPTN_HUMAN Optineurin (Optic neuropathy-inducing ... 30 2.2
sp|P53564|CUTL1_MOUSE CCAAT displacement protein (CDP) (Cut... 30 2.2
sp|P47481|LON_MYCGE ATP-dependent protease La 30 2.9
sp|Q9VJE5|CL190_DROME Restin homolog (Cytoplasmic linker pr... 30 2.9
>sp|Q13503|MED21_HUMAN Mediator of RNA polymerase II transcription subunit 21 (RNA
polymerase II holoenzyme component SRB7) (RNAPII complex
component SRB7)
Length = 144
Score = 52.8 bits (125), Expect = 4e-07
Identities = 34/125 (27%), Positives = 59/125 (47%)
Frame = +2
Query: 5 CDSVGVIQQFAQPSFFGEFDWKARSTRTDNAIIQSVLKDPPPPDYSKQFAKVIRETAAQI 184
C+++GV+QQ P+ F + N P +Y++ FA +I TA I
Sbjct: 20 CNAIGVLQQCGPPASFNNIQTAINKDQPAN----------PTEEYAQLFAALIARTAKDI 69
Query: 185 NYLIDGLPEEDLSLEIHNSALSLLHNDYIESGKELLKTVDNLEKKLEFVREKLVEISEVQ 364
+ LID LP E+ + + ++L L + E+ L V + LE ++ L +I++ Q
Sbjct: 70 DVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQ 129
Query: 365 LTTEA 379
L T +
Sbjct: 130 LKTRS 134
>sp|Q13201|MMRN1_HUMAN Multimerin-1 precursor (Endothelial cell multimerin 1) (EMILIN-4)
(Elastin microfibril interface located protein 4)
(Elastin microfibril interfacer 4)
Length = 1228
Score = 36.2 bits (82), Expect = 0.041
Identities = 22/90 (24%), Positives = 45/90 (50%), Gaps = 3/90 (3%)
Frame = +2
Query: 137 YSKQFAKVIRETAAQINYLIDGLPEEDLSL---EIHNSALSLLHNDYIESGKELLKTVDN 307
YS KV + + + L+ GL + +++ +I + ND E+ +L KTV +
Sbjct: 356 YSSLEGKVSEDKSREFQSLLKGLKSKSINVLIRDIVREQFKIFQNDMQETVAQLFKTVSS 415
Query: 308 LEKKLEFVREKLVEISEVQLTTEALEAEIL 397
L + LE R+ + +++E ++ A + +L
Sbjct: 416 LSEDLESTRQIIQKVNESVVSIAAQQKFVL 445
>sp|P47822|MED21_YEAST RNA polymerase II mediator complex subunit 21 (RNAPII complex
component SRB7) (Suppressor of RNA polymerase B 7)
Length = 140
Score = 34.3 bits (77), Expect = 0.15
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 3/78 (3%)
Frame = +2
Query: 125 PPPDYSKQFAKVIRET---AAQINYLIDGLPEEDLSLEIHNSALSLLHNDYIESGKELLK 295
PP ++S ++ + QIN LID LP D+S E + +L +E E ++
Sbjct: 51 PPEEFSNTIDELSTDIILKTRQINKLIDSLPGVDVSAEEQLRKIDMLQKKLVEVEDEKIE 110
Query: 296 TVDNLEKKLEFVREKLVE 349
+ EK L V + L+E
Sbjct: 111 AIKKKEKLLRHV-DSLIE 127
>sp|Q8JFQ6|K1C13_ONCMY Keratin, type I cytoskeletal 13 (Cytokeratin-13) (CK-13)
(Keratin-13) (K13)
Length = 492
Score = 31.6 bits (70), Expect = 1.00
Identities = 24/94 (25%), Positives = 44/94 (46%), Gaps = 7/94 (7%)
Frame = +2
Query: 134 DYSKQFAKVIRETAAQINYLIDGLPEEDLS---LEIHNSALSLLHNDYIESGKELLKTVD 304
++ ++ + + QIN +D P+EDLS EI S+ + + +
Sbjct: 254 NHEEELLAMRTQMTGQINVEVDAAPQEDLSRVMAEIREQYESVSAKNQRDLEAWFQTKTE 313
Query: 305 NLEKKL----EFVREKLVEISEVQLTTEALEAEI 394
L K++ E ++ EISE++ T +ALE E+
Sbjct: 314 TLNKEVASSTEVLQTSKSEISEIRRTLQALEIEL 347
>sp|P40813|T1M_SALTY Type I restriction enzyme StySJI M protein (M.StySJI)
Length = 529
Score = 30.8 bits (68), Expect = 1.7
Identities = 18/79 (22%), Positives = 37/79 (46%)
Frame = +2
Query: 149 FAKVIRETAAQINYLIDGLPEEDLSLEIHNSALSLLHNDYIESGKELLKTVDNLEKKLEF 328
F K+ +ET + +YL +G +DL I + N ++ G + K V + +
Sbjct: 36 FLKMCKETGQEADYLPEGYRWDDLKSRIGQDQMQFYRNLLVQLGSDEKKLVQAVFHNVST 95
Query: 329 VREKLVEISEVQLTTEALE 385
E+ +++E+ +AL+
Sbjct: 96 TIEQPKQLTELVSYMDALD 114
>sp|Q9P2G1|AKIB1_HUMAN Ankyrin repeat and IBR domain containing protein 1
Length = 1214
Score = 30.8 bits (68), Expect = 1.7
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 32 FAQPSFFGEFDWKARSTRTDNAIIQSVLKDPPPPD 136
FA P + EF ++ R + + S+L +PP PD
Sbjct: 882 FASPEEYAEFQYRRRHRQRRRGDVHSLLSNPPDPD 916
>sp|Q96CV9|OPTN_HUMAN Optineurin (Optic neuropathy-inducing protein)
(E3-14.7K-interacting protein) (FIP-2)
(Huntingtin-interacting protein HYPL) (NEMO-related
protein) (Transcription factor IIIA-interacting protein)
(TFIIIA-IntP)
Length = 577
Score = 30.4 bits (67), Expect = 2.2
Identities = 28/124 (22%), Positives = 56/124 (45%), Gaps = 12/124 (9%)
Frame = +2
Query: 68 KARSTRTDNAIIQSVLKDPPPPDYSKQFAKVIRETAAQINYLIDGLPEEDLSLEIHNSAL 247
K ++ N+ I S L + Y+ ++ Q+ ++ + E E S L
Sbjct: 333 KCQALERKNSAIPSELNEKQELVYTN------KKLELQVESMLSEIKMEQAKTEDEKSKL 386
Query: 248 SLL---HNDYIESGKELLKTVDNLEKK---------LEFVREKLVEISEVQLTTEALEAE 391
++L HN ++ LKT++ L +K L+ + EKL E++E L ++ L+ +
Sbjct: 387 TVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKL-ELAEKALASKQLQMD 445
Query: 392 ILKK 403
+K+
Sbjct: 446 EMKQ 449
>sp|P53564|CUTL1_MOUSE CCAAT displacement protein (CDP) (Cut-like 1) (Homeobox protein
Cux)
Length = 1515
Score = 30.4 bits (67), Expect = 2.2
Identities = 27/112 (24%), Positives = 51/112 (45%), Gaps = 5/112 (4%)
Frame = +2
Query: 86 TDNAIIQSVLKDPPPPDYSKQFAKVIRETAAQINYLIDGLPEEDLSLEIHNSALSL---- 253
T+N ++ L++ Y+K+FA+V + I L + + E + +L+ ++L
Sbjct: 113 TENQKLRETLEE-----YNKEFAEV-KNQEVTIKALKEKIREYEQTLKSQAETIALEKEQ 166
Query: 254 -LHNDYIESGKELLKTVDNLEKKLEFVREKLVEISEVQLTTEALEAEILKKY 406
L ND+ E ++L +T + KLE KL + T ++ KY
Sbjct: 167 KLQNDFAEKERKLQETQMSTTSKLEEAEHKLQTLQTALEKTRTELFDLKTKY 218
>sp|P47481|LON_MYCGE ATP-dependent protease La
Length = 795
Score = 30.0 bits (66), Expect = 2.9
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = +2
Query: 281 KELLKTVDNLEKKLEFVREKLVEISEVQLTTEALEAEIL 397
K L+KT+D L+ V+E++VE VQL T+ + I+
Sbjct: 337 KNLIKTLDKNHTGLDKVKERIVEYLAVQLRTQKNKGPIM 375
>sp|Q9VJE5|CL190_DROME Restin homolog (Cytoplasmic linker protein 190) (Microtubule binding
protein 190) (d-CLIP-190)
Length = 1690
Score = 30.0 bits (66), Expect = 2.9
Identities = 22/87 (25%), Positives = 41/87 (47%), Gaps = 1/87 (1%)
Frame = +2
Query: 134 DYSKQFAKVIRETAAQINYLIDGLPEEDLSLEIHNSALSLLHNDYIESGKELLKTVDNLE 313
+ K+ +I+E ++ L E + +L++ L L SG+E KTV L
Sbjct: 801 ELKKEKETIIKEKEQELQQLQSKSAESESALKVVQVQLEQLQQQAAASGEEGSKTVAKLH 860
Query: 314 KKLEFVREKLVEI-SEVQLTTEALEAE 391
++ ++ + E SE++ T LEA+
Sbjct: 861 DEISQLKSQAEETQSELKSTQSNLEAK 887
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,274,210
Number of Sequences: 369166
Number of extensions: 702645
Number of successful extensions: 2722
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2615
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2717
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2871696580
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)