Planarian EST Database


Dr_sW_016_M09

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_016_M09
         (318 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P27797|CRTC_HUMAN  Calreticulin precursor (CRP55) (Calreg...    77   1e-14
sp|P42918|CRTC2_BOVIN  Calreticulin, brain isoform 2 precurs...    75   6e-14
sp|P52193|CRTC1_BOVIN  Calreticulin, brain isoform 1 precurs...    75   6e-14
sp|P18418|CRTC_RAT  Calreticulin precursor (CRP55) (Calregul...    73   2e-13
sp|P14211|CRTC_MOUSE  Calreticulin precursor (CRP55) (Calreg...    73   2e-13
sp|Q8K3H7|CRTC_CRIGR  Calreticulin precursor (CRP55) (Calreg...    73   2e-13
sp|P15253|CRTC_RABIT  Calreticulin precursor (CRP55) (Calreg...    72   5e-13
sp|Q9D9Q6|CRTC3_MOUSE  Calreticulin-3 precursor (Calreticuli...    69   3e-12
sp|P11012|RAL1_ONCVO  RAL-1 protein precursor (RAL1 antigen)...    67   2e-11
sp|P29413|CRTC_DROME  Calreticulin precursor (CRP55) (Calreg...    66   2e-11
>sp|P27797|CRTC_HUMAN Calreticulin precursor (CRP55) (Calregulin) (HACBP) (ERp60) (grp60)
          Length = 417

 Score = 77.0 bits (188), Expect = 1e-14
 Identities = 34/53 (64%), Positives = 42/53 (79%)
 Frame = +1

Query: 103 NIGSIGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDKQD 261
           N G +G DLWQVKSGTIFDN L+T+D AYA+++  +TW  T A EK+MKDKQD
Sbjct: 310 NFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQD 362

 Score = 54.7 bits (130), Expect = 7e-08
 Identities = 20/27 (74%), Positives = 24/27 (88%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPE 82
           +P+QIDNP YKG WIHPEIDNP+Y P+
Sbjct: 276 KPRQIDNPDYKGTWIHPEIDNPEYSPD 302
>sp|P42918|CRTC2_BOVIN Calreticulin, brain isoform 2 precursor (CRP55) (Calregulin)
           (HACBP)
          Length = 421

 Score = 74.7 bits (182), Expect = 6e-14
 Identities = 33/53 (62%), Positives = 41/53 (77%)
 Frame = +1

Query: 103 NIGSIGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDKQD 261
           N   +G DLWQVKSGTIFDN L+T+D AYA+++  +TW  T A EK+MKDKQD
Sbjct: 314 NFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQD 366

 Score = 58.5 bits (140), Expect = 5e-09
 Identities = 22/29 (75%), Positives = 27/29 (93%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPESN 88
           +P+QIDNP+YKG WIHPEIDNP+Y P+SN
Sbjct: 280 KPRQIDNPEYKGIWIHPEIDNPEYSPDSN 308
>sp|P52193|CRTC1_BOVIN Calreticulin, brain isoform 1 precursor (CRP55) (Calregulin)
           (HACBP)
          Length = 417

 Score = 74.7 bits (182), Expect = 6e-14
 Identities = 33/53 (62%), Positives = 41/53 (77%)
 Frame = +1

Query: 103 NIGSIGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDKQD 261
           N   +G DLWQVKSGTIFDN L+T+D AYA+++  +TW  T A EK+MKDKQD
Sbjct: 310 NFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQD 362

 Score = 58.5 bits (140), Expect = 5e-09
 Identities = 22/29 (75%), Positives = 27/29 (93%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPESN 88
           +P+QIDNP+YKG WIHPEIDNP+Y P+SN
Sbjct: 276 KPRQIDNPEYKGIWIHPEIDNPEYSPDSN 304
>sp|P18418|CRTC_RAT Calreticulin precursor (CRP55) (Calregulin) (HACBP) (ERp60) (CALBP)
           (Calcium-binding protein 3) (CABP3)
          Length = 416

 Score = 73.2 bits (178), Expect = 2e-13
 Identities = 32/49 (65%), Positives = 40/49 (81%)
 Frame = +1

Query: 115 IGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDKQD 261
           +G DLWQVKSGTIFDN L+T+D AYA+++  +TW  T A EK+MKDKQD
Sbjct: 314 LGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQD 362

 Score = 57.4 bits (137), Expect = 1e-08
 Identities = 21/29 (72%), Positives = 26/29 (89%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPESN 88
           +P+QIDNP YKG WIHPEIDNP+Y P++N
Sbjct: 276 KPRQIDNPDYKGTWIHPEIDNPEYSPDAN 304
>sp|P14211|CRTC_MOUSE Calreticulin precursor (CRP55) (Calregulin) (HACBP) (ERp60)
          Length = 416

 Score = 73.2 bits (178), Expect = 2e-13
 Identities = 32/49 (65%), Positives = 40/49 (81%)
 Frame = +1

Query: 115 IGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDKQD 261
           +G DLWQVKSGTIFDN L+T+D AYA+++  +TW  T A EK+MKDKQD
Sbjct: 314 LGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQD 362

 Score = 57.4 bits (137), Expect = 1e-08
 Identities = 21/29 (72%), Positives = 26/29 (89%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPESN 88
           +P+QIDNP YKG WIHPEIDNP+Y P++N
Sbjct: 276 KPRQIDNPDYKGTWIHPEIDNPEYSPDAN 304
>sp|Q8K3H7|CRTC_CRIGR Calreticulin precursor (CRP55) (Calregulin) (HACBP) (ERp60)
          Length = 417

 Score = 73.2 bits (178), Expect = 2e-13
 Identities = 32/49 (65%), Positives = 40/49 (81%)
 Frame = +1

Query: 115 IGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDKQD 261
           +G DLWQVKSGTIFDN L+T+D AYA+++  +TW  T A EK+MKDKQD
Sbjct: 314 LGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKASEKQMKDKQD 362

 Score = 57.4 bits (137), Expect = 1e-08
 Identities = 21/29 (72%), Positives = 26/29 (89%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPESN 88
           +P+QIDNP YKG WIHPEIDNP+Y P++N
Sbjct: 276 KPRQIDNPDYKGTWIHPEIDNPEYSPDAN 304
>sp|P15253|CRTC_RABIT Calreticulin precursor (CRP55) (Calregulin) (HACBP) (ERp60)
          Length = 418

 Score = 71.6 bits (174), Expect = 5e-13
 Identities = 31/49 (63%), Positives = 39/49 (79%)
 Frame = +1

Query: 115 IGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDKQD 261
           +G DLWQVKSGTIFDN L+T+D AYA+++  +TW  T   EK+MKDKQD
Sbjct: 314 LGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKTAEKQMKDKQD 362

 Score = 57.4 bits (137), Expect = 1e-08
 Identities = 21/29 (72%), Positives = 26/29 (89%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPESN 88
           +P+QIDNP YKG WIHPEIDNP+Y P++N
Sbjct: 276 KPRQIDNPDYKGTWIHPEIDNPEYSPDAN 304
>sp|Q9D9Q6|CRTC3_MOUSE Calreticulin-3 precursor (Calreticulin-2)
          Length = 380

 Score = 68.9 bits (167), Expect = 3e-12
 Identities = 30/48 (62%), Positives = 38/48 (79%)
 Frame = +1

Query: 103 NIGSIGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKM 246
           NIG+IG +LWQV+SGTIFDN L+TDD  YA+K+ + TW ET   EK+M
Sbjct: 293 NIGAIGLELWQVRSGTIFDNFLITDDEEYAEKFGKATWGETKGPEKEM 340
>sp|P11012|RAL1_ONCVO RAL-1 protein precursor (RAL1 antigen) (41 kDa larval antigen)
          Length = 388

 Score = 66.6 bits (161), Expect = 2e-11
 Identities = 32/51 (62%), Positives = 39/51 (76%)
 Frame = +1

Query: 103 NIGSIGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDK 255
           +IG+IGFDLWQVKSGTIFD++++TD    AKK+ EKT K T  GEKK   K
Sbjct: 308 DIGAIGFDLWQVKSGTIFDDVIVTDSVEEAKKFGEKTLKITREGEKKKGKK 358

 Score = 48.9 bits (115), Expect = 4e-06
 Identities = 19/29 (65%), Positives = 22/29 (75%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPESN 88
           +PKQ  NP YKG WIHPEI+ PDY P+ N
Sbjct: 274 KPKQKKNPAYKGKWIHPEIEIPDYTPDDN 302
>sp|P29413|CRTC_DROME Calreticulin precursor (CRP55) (Calregulin) (HACBP)
          Length = 406

 Score = 66.2 bits (160), Expect = 2e-11
 Identities = 33/52 (63%), Positives = 39/52 (75%)
 Frame = +1

Query: 106 IGSIGFDLWQVKSGTIFDNILLTDDPAYAKKYVEKTWKETAAGEKKMKDKQD 261
           I ++GFDLWQVKSGTIFDN+L+TDD   A K   +  K T AGEKKMK+ QD
Sbjct: 311 ICTLGFDLWQVKSGTIFDNVLITDDVELAAKAAAEV-KNTQAGEKKMKEAQD 361

 Score = 49.3 bits (116), Expect = 3e-06
 Identities = 18/27 (66%), Positives = 22/27 (81%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYKPE 82
           +PKQ+DNP YKG W HPEI NP+Y P+
Sbjct: 276 QPKQLDNPNYKGAWEHPEIANPEYVPD 302

 Score = 38.9 bits (89), Expect = 0.004
 Identities = 14/25 (56%), Positives = 19/25 (76%)
 Frame = +2

Query: 2   EPKQIDNPKYKGPWIHPEIDNPDYK 76
           EP  IDNP++KG W   ++DNP+YK
Sbjct: 262 EPPMIDNPEFKGEWQPKQLDNPNYK 286
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,809,263
Number of Sequences: 369166
Number of extensions: 437117
Number of successful extensions: 1462
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1325
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1461
length of database: 68,354,980
effective HSP length: 74
effective length of database: 54,684,590
effective search space used: 1695222290
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)