Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_M03
(789 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P70711|UB2D4_RAT Ubiquitin-conjugating enzyme E2 D4 (Ubi... 119 7e-27
sp|P61080|UB2D1_MOUSE Ubiquitin-conjugating enzyme E2 D1 (U... 118 2e-26
sp|P46595|UBC4_SCHPO Ubiquitin-conjugating enzyme E2 4 (Ubi... 117 4e-26
sp|P15732|UBC5_YEAST Ubiquitin-conjugating enzyme E2-16 kDa... 116 6e-26
sp|P62839|UB2D2_RAT Ubiquitin-conjugating enzyme E2 D2 (Ubi... 115 1e-25
sp|O74196|UBC1_COLGL Ubiquitin-conjugating enzyme E2-16 kDa... 114 2e-25
sp|P35129|UBC2_CAEEL Ubiquitin-conjugating enzyme E2 2 (Ubi... 114 3e-25
sp|P25867|UBCD1_DROME Ubiquitin-conjugating enzyme E2-17 kD... 114 4e-25
sp|P61078|UB2D3_RAT Ubiquitin-conjugating enzyme E2 D3 (Ubi... 114 4e-25
sp|P35134|UBCB_ARATH Ubiquitin-conjugating enzyme E2-17 kDa... 113 7e-25
>sp|P70711|UB2D4_RAT Ubiquitin-conjugating enzyme E2 D4 (Ubiquitin-protein ligase D4)
(Ubiquitin carrier protein D4) (Ubiquitin-conjugating
enzyme E2-17 kDa 4) (E2(17)KB 4)
Length = 147
Score = 119 bits (299), Expect = 7e-27
Identities = 53/81 (65%), Positives = 64/81 (79%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K+ F TRIYHPN+N+ GSICL+IL +WSPALT+SKVLL IS+LL DPNPDDP P IAQ
Sbjct: 66 KVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQ 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K D+ + R A EWT KYA
Sbjct: 126 IYKTDRDKYNRTAREWTQKYA 146
Score = 49.3 bits (116), Expect = 1e-05
Identities = 20/37 (54%), Positives = 30/37 (81%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R++KEL + +PPAQC+AGP+ E +M+ W+AT+MGP
Sbjct: 5 RIHKELNDLAQDPPAQCSAGPVGE-DMFHWQATIMGP 40
Score = 42.4 bits (98), Expect = 0.001
Identities = 20/37 (54%), Positives = 23/37 (62%)
Frame = +3
Query: 138 WHYLIIKGN*VGSPYEGGIFFLDIVFPEKYPFSPPAV 248
W I+ N SPY+GG FFL I FP +YPF PP V
Sbjct: 33 WQATIMGPN--DSPYQGGAFFLTIDFPTEYPFKPPKV 67
>sp|P61080|UB2D1_MOUSE Ubiquitin-conjugating enzyme E2 D1 (Ubiquitin-protein ligase D1)
(Ubiquitin carrier protein D1) (Ubiquitin-conjugating
enzyme E2-17 kDa 1) (E2(17)KB 1)
sp|P51668|UB2D1_HUMAN Ubiquitin-conjugating enzyme E2 D1 (Ubiquitin-protein ligase D1)
(Ubiquitin carrier protein D1) (UbcH5)
(Ubiquitin-conjugating enzyme E2-17 kDa 1) (E2(17)KB 1)
Length = 147
Score = 118 bits (295), Expect = 2e-26
Identities = 54/81 (66%), Positives = 64/81 (79%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
KI F T+IYHPNIN+ GSICL+IL +WSPALT+SKVLL I +LL DPNPDDP P IAQ
Sbjct: 66 KIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQ 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K DK+ + R A EWT KYA
Sbjct: 126 IYKSDKEKYNRHAREWTQKYA 146
Score = 45.4 bits (106), Expect = 2e-04
Identities = 17/37 (45%), Positives = 29/37 (78%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R+ KEL + + +PPA C+AGP+ + +++ W+AT+MGP
Sbjct: 5 RIQKELSDLQRDPPAHCSAGPVGD-DLFHWQATIMGP 40
Score = 39.7 bits (91), Expect = 0.010
Identities = 18/46 (39%), Positives = 24/46 (52%)
Frame = +3
Query: 129 DYLWHYLIIKGN*VGSPYEGGIFFLDIVFPEKYPFSPPAVILYNSI 266
D L+H+ S Y+GG+FFL + FP YPF PP + I
Sbjct: 28 DDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKI 73
>sp|P46595|UBC4_SCHPO Ubiquitin-conjugating enzyme E2 4 (Ubiquitin-protein ligase 4)
(Ubiquitin carrier protein 4)
Length = 147
Score = 117 bits (293), Expect = 4e-26
Identities = 53/81 (65%), Positives = 63/81 (77%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K+NF TRIYHPNIN+ GSICL+IL +WSPALT+SKVLL I +LL DPNPDDP P IA
Sbjct: 66 KVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAH 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K D+ +E A EWT KYA
Sbjct: 126 VYKTDRSRYELSAREWTRKYA 146
Score = 43.9 bits (102), Expect = 5e-04
Identities = 16/37 (43%), Positives = 29/37 (78%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R+N+EL + +PP+ C+AGP+ + +++ W+AT+MGP
Sbjct: 5 RINRELADLGKDPPSSCSAGPVGD-DLFHWQATIMGP 40
Score = 43.1 bits (100), Expect = 9e-04
Identities = 20/40 (50%), Positives = 23/40 (57%)
Frame = +3
Query: 129 DYLWHYLIIKGN*VGSPYEGGIFFLDIVFPEKYPFSPPAV 248
D L+H+ SPY GG+FFL I FP YPF PP V
Sbjct: 28 DDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKV 67
>sp|P15732|UBC5_YEAST Ubiquitin-conjugating enzyme E2-16 kDa (Ubiquitin-protein ligase)
(Ubiquitin carrier protein)
Length = 148
Score = 116 bits (291), Expect = 6e-26
Identities = 53/81 (65%), Positives = 64/81 (79%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K+NF T+IYHPNIN++G+ICL+IL +WSPALTLSKVLL I +LL D NPDDP P IAQ
Sbjct: 67 KVNFTTKIYHPNINSSGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQ 126
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K DK +E A EWT KYA
Sbjct: 127 IYKTDKAKYEATAKEWTKKYA 147
Score = 43.5 bits (101), Expect = 7e-04
Identities = 17/37 (45%), Positives = 28/37 (75%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R+ KEL + +PPA C+AGP+ + ++Y W+A++MGP
Sbjct: 6 RIAKELSDLGRDPPASCSAGPVGD-DLYHWQASIMGP 41
Score = 42.4 bits (98), Expect = 0.001
Identities = 20/40 (50%), Positives = 23/40 (57%)
Frame = +3
Query: 129 DYLWHYLIIKGN*VGSPYEGGIFFLDIVFPEKYPFSPPAV 248
D L+H+ SPY GG+FFL I FP YPF PP V
Sbjct: 29 DDLYHWQASIMGPSDSPYAGGVFFLSIHFPTDYPFKPPKV 68
>sp|P62839|UB2D2_RAT Ubiquitin-conjugating enzyme E2 D2 (Ubiquitin-protein ligase D2)
(Ubiquitin carrier protein D2) (Ubiquitin-conjugating
enzyme E2-17 kDa 2) (E2(17)KB 2)
sp|P62838|UB2D2_MOUSE Ubiquitin-conjugating enzyme E2 D2 (Ubiquitin-protein ligase D2)
(Ubiquitin carrier protein D2) (Ubiquitin-conjugating
enzyme E2-17 kDa 2) (E2(17)KB 2)
sp|P62837|UB2D2_HUMAN Ubiquitin-conjugating enzyme E2 D2 (Ubiquitin-protein ligase D2)
(Ubiquitin carrier protein D2) (Ubiquitin-conjugating
enzyme E2-17 kDa 2) (E2(17)KB 2)
sp|P62840|UB5B_XENLA Ubiquitin-conjugating enzyme E2 D2 (Ubiquitin-protein ligase D2)
(Ubiquitin carrier protein D2) (Xubc4)
Length = 147
Score = 115 bits (289), Expect = 1e-25
Identities = 52/81 (64%), Positives = 64/81 (79%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K+ F TRIYHPNIN+ GSICL+IL +WSPALT+SKVLL I +LL DPNPDDP P IA+
Sbjct: 66 KVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIAR 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K D++ + R A EWT KYA
Sbjct: 126 IYKTDREKYNRIAREWTQKYA 146
Score = 48.1 bits (113), Expect = 3e-05
Identities = 19/37 (51%), Positives = 30/37 (81%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R++KEL + +PPAQC+AGP+ + +M+ W+AT+MGP
Sbjct: 5 RIHKELNDLARDPPAQCSAGPVGD-DMFHWQATIMGP 40
Score = 43.1 bits (100), Expect = 9e-04
Identities = 20/37 (54%), Positives = 23/37 (62%)
Frame = +3
Query: 138 WHYLIIKGN*VGSPYEGGIFFLDIVFPEKYPFSPPAV 248
W I+ N SPY+GG+FFL I FP YPF PP V
Sbjct: 33 WQATIMGPN--DSPYQGGVFFLTIHFPTDYPFKPPKV 67
>sp|O74196|UBC1_COLGL Ubiquitin-conjugating enzyme E2-16 kDa (Ubiquitin-protein ligase)
(Ubiquitin carrier protein) (Colletotrichum
hard-surface-induced protein 1)
Length = 147
Score = 114 bits (286), Expect = 2e-25
Identities = 51/81 (62%), Positives = 63/81 (77%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K+NF TRIYHPNIN+ GSICL+IL +WSPALT+SKVLL I ++L DPNPD+P P IA
Sbjct: 66 KVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDEPLVPEIAH 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K D+ +E A EWT KYA
Sbjct: 126 VYKTDRARYEATAREWTRKYA 146
Score = 46.2 bits (108), Expect = 1e-04
Identities = 18/37 (48%), Positives = 29/37 (78%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R+NKEL + +PP+ C+AGP+ E +++ W+AT+MGP
Sbjct: 5 RINKELTDLGRDPPSSCSAGPVGE-DLFHWQATIMGP 40
Score = 41.6 bits (96), Expect = 0.003
Identities = 17/25 (68%), Positives = 18/25 (72%)
Frame = +3
Query: 174 SPYEGGIFFLDIVFPEKYPFSPPAV 248
SPY GG+FFL I FP YPF PP V
Sbjct: 43 SPYSGGVFFLAIHFPTDYPFKPPKV 67
>sp|P35129|UBC2_CAEEL Ubiquitin-conjugating enzyme E2 2 (Ubiquitin-protein ligase 2)
(Ubiquitin carrier protein 2)
Length = 147
Score = 114 bits (285), Expect = 3e-25
Identities = 51/81 (62%), Positives = 64/81 (79%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K+ F TRIYHPNIN+ GSICL+IL +WSPALT+SKVLL I +LL DPNPDDP P IA+
Sbjct: 66 KVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIAR 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K D++ + + A EWT KYA
Sbjct: 126 IYKTDRERYNQLAREWTQKYA 146
Score = 48.1 bits (113), Expect = 3e-05
Identities = 19/37 (51%), Positives = 30/37 (81%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R+ KELQ+ +PPAQC+AGP+ + +++ W+AT+MGP
Sbjct: 5 RIQKELQDLGRDPPAQCSAGPVGD-DLFHWQATIMGP 40
Score = 42.7 bits (99), Expect = 0.001
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = +3
Query: 174 SPYEGGIFFLDIVFPEKYPFSPPAV 248
SPY+GG+FFL I FP YPF PP V
Sbjct: 43 SPYQGGVFFLTIHFPTDYPFKPPKV 67
>sp|P25867|UBCD1_DROME Ubiquitin-conjugating enzyme E2-17 kDa (Ubiquitin-protein ligase)
(Ubiquitin carrier protein) (Effete protein)
Length = 147
Score = 114 bits (284), Expect = 4e-25
Identities = 51/81 (62%), Positives = 63/81 (77%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K+ F TRIYHPNIN+ GSICL+IL +WSPALT+SKVLL I +LL DPNPDDP P IA+
Sbjct: 66 KVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIAR 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K D++ + A EWT KYA
Sbjct: 126 IYKTDREKYNELAREWTRKYA 146
Score = 50.4 bits (119), Expect = 5e-06
Identities = 20/37 (54%), Positives = 31/37 (83%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R+NKELQ+ +PPAQC+AGP+ + +++ W+AT+MGP
Sbjct: 5 RINKELQDLGRDPPAQCSAGPVGD-DLFHWQATIMGP 40
Score = 43.1 bits (100), Expect = 9e-04
Identities = 20/40 (50%), Positives = 24/40 (60%)
Frame = +3
Query: 129 DYLWHYLIIKGN*VGSPYEGGIFFLDIVFPEKYPFSPPAV 248
D L+H+ SPY+GG+FFL I FP YPF PP V
Sbjct: 28 DDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYPFKPPKV 67
>sp|P61078|UB2D3_RAT Ubiquitin-conjugating enzyme E2 D3 (Ubiquitin-protein ligase D3)
(Ubiquitin carrier protein D3) (Ubiquitin-conjugating
enzyme E2-17 kDa 3) (E2(17)KB 3) (Phosphoarginine
phosphatase) (PAPase)
sp|P61079|UB2D3_MOUSE Ubiquitin-conjugating enzyme E2 D3 (Ubiquitin-protein ligase D3)
(Ubiquitin carrier protein D3) (Ubiquitin-conjugating
enzyme E2-17 kDa 3) (E2(17)KB 3)
sp|P61077|UB2D3_HUMAN Ubiquitin-conjugating enzyme E2 D3 (Ubiquitin-protein ligase D3)
(Ubiquitin carrier protein D3) (Ubiquitin-conjugating
enzyme E2-17 kDa 3) (E2(17)KB 3)
Length = 147
Score = 114 bits (284), Expect = 4e-25
Identities = 51/81 (62%), Positives = 63/81 (77%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K+ F TRIYHPNIN+ GSICL+IL +WSPALT+SKVLL I +LL DPNPDDP P IA+
Sbjct: 66 KVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIAR 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K D+ + R + EWT KYA
Sbjct: 126 IYKTDRDKYNRISREWTQKYA 146
Score = 50.1 bits (118), Expect = 7e-06
Identities = 20/37 (54%), Positives = 30/37 (81%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R+NKEL + +PPAQC+AGP+ + +M+ W+AT+MGP
Sbjct: 5 RINKELSDLARDPPAQCSAGPVGD-DMFHWQATIMGP 40
Score = 43.1 bits (100), Expect = 9e-04
Identities = 20/37 (54%), Positives = 23/37 (62%)
Frame = +3
Query: 138 WHYLIIKGN*VGSPYEGGIFFLDIVFPEKYPFSPPAV 248
W I+ N SPY+GG+FFL I FP YPF PP V
Sbjct: 33 WQATIMGPN--DSPYQGGVFFLTIHFPTDYPFKPPKV 67
>sp|P35134|UBCB_ARATH Ubiquitin-conjugating enzyme E2-17 kDa 11 (Ubiquitin-protein ligase
11) (Ubiquitin carrier protein 11)
Length = 148
Score = 113 bits (282), Expect = 7e-25
Identities = 49/81 (60%), Positives = 63/81 (77%)
Frame = +1
Query: 286 KINFITRIYHPNINTTGSICLNILNKEWSPALTLSKVLLCISALLVDPNPDDPYSPAIAQ 465
K++F T++YHPNIN+ GSICL+IL ++WSPALT+SKVLL I +LL DPNPDDP P IA
Sbjct: 66 KVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAH 125
Query: 466 LFKEDKKSFERKAAEWTYKYA 528
++K D+ +E A WT KYA
Sbjct: 126 MYKTDRSKYESTARSWTQKYA 146
Score = 45.1 bits (105), Expect = 2e-04
Identities = 18/37 (48%), Positives = 30/37 (81%)
Frame = +2
Query: 14 RLNKELQEFRFNPPAQCTAGPINESNMYKWRATLMGP 124
R+ KEL++ + +PP+ C+AGP+ E +M+ W+AT+MGP
Sbjct: 5 RILKELKDLQKDPPSNCSAGPVAE-DMFHWQATIMGP 40
Score = 38.5 bits (88), Expect = 0.021
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +3
Query: 174 SPYEGGIFFLDIVFPEKYPFSPPAV 248
SPY GG+F + I FP YPF PP V
Sbjct: 43 SPYAGGVFLVSIHFPPDYPFKPPKV 67
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,830,350
Number of Sequences: 369166
Number of extensions: 1335730
Number of successful extensions: 3391
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3099
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3321
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7454154400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)