Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_L16
(824 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q10901|EAA1_CAEEL Excitatory amino acid transporter (Sod... 253 3e-67
sp|P43004|EAA2_HUMAN Excitatory amino acid transporter 2 (S... 248 2e-65
sp|Q25605|EAAT_ONCVO Excitatory amino acid transporter (Sod... 247 2e-65
sp|P43006|EAA2_MOUSE Excitatory amino acid transporter 2 (S... 247 3e-65
sp|P31596|EAA2_RAT Excitatory amino acid transporter 2 (Sod... 247 3e-65
sp|P43003|EAA1_HUMAN Excitatory amino acid transporter 1 (S... 244 3e-64
sp|P46411|EAA1_BOVIN Excitatory amino acid transporter 1 (S... 244 3e-64
sp|P24942|EAA1_RAT Excitatory amino acid transporter 1 (Sod... 243 5e-64
sp|P56564|EAA1_MOUSE Excitatory amino acid transporter 1 (S... 243 5e-64
sp|O57321|EAA1_AMBTI Excitatory amino acid transporter 1 (S... 239 5e-63
>sp|Q10901|EAA1_CAEEL Excitatory amino acid transporter (Sodium-dependent glutamate/
aspartate transporter)
Length = 503
Score = 253 bits (647), Expect = 3e-67
Identities = 126/167 (75%), Positives = 151/167 (90%), Gaps = 1/167 (0%)
Frame = -3
Query: 705 YIYQKESIKVF-HGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGATI 529
++ K++ VF G+ QAW+TALGTASSSATLPITF CLEEN GVD+RVTRFVLP+GATI
Sbjct: 300 FVTTKKNPYVFMRGLFQAWITALGTASSSATLPITFNCLEENLGVDRRVTRFVLPVGATI 359
Query: 528 NMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVLTS 349
NMDGTALYEAVA+IFIAQING++L+FGQ+ T+S+TATLA+IGAASVPSAGLVTM+LVLT+
Sbjct: 360 NMDGTALYEAVAAIFIAQINGVHLSFGQVVTVSLTATLASIGAASVPSAGLVTMLLVLTA 419
Query: 348 VGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELEA 208
VGLP+ D++LI+AVDWLLDR+RTSINVLGDA GAGIV H K +L+A
Sbjct: 420 VGLPVKDVSLIVAVDWLLDRIRTSINVLGDAMGAGIVYHYSKADLDA 466
Score = 63.9 bits (154), Expect = 5e-10
Identities = 33/47 (70%), Positives = 37/47 (78%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNP 684
GKILEI DLA+TA L YMVTVL GLAIH I+L LIFF+ T+KNP
Sbjct: 261 GKILEIHDLADTARMLAMYMVTVLSGLAIHSLISLPLIFFVTTKKNP 307
>sp|P43004|EAA2_HUMAN Excitatory amino acid transporter 2 (Sodium-dependent
glutamate/aspartate transporter 2)
Length = 574
Score = 248 bits (632), Expect = 2e-65
Identities = 118/169 (69%), Positives = 147/169 (86%)
Frame = -3
Query: 711 FLYIYQKESIKVFHGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGAT 532
+ + +K F G+ QAW+TALGTASS+ TLP+TF+CLEEN G+DKRVTRFVLP+GAT
Sbjct: 336 YFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGAT 395
Query: 531 INMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVLT 352
INMDGTALYEAVA+IFIAQ+NG+ L GQI T+S+TATLA++GAAS+PSAGLVTM+L+LT
Sbjct: 396 INMDGTALYEAVAAIFIAQMNGVVLDGGQIVTVSLTATLASVGAASIPSAGLVTMLLILT 455
Query: 351 SVGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELEAI 205
+VGLP DI+L++AVDWLLDR+RTS+NV+GD+FGAGIV HL K EL+ I
Sbjct: 456 AVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTI 504
Score = 68.6 bits (166), Expect = 2e-11
Identities = 32/56 (57%), Positives = 40/56 (71%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNPLKYFTECYK 657
GKI+ IKDL A LG YMVTV++GL IHGGI L LI+F+ TRKNP +F ++
Sbjct: 298 GKIIAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQ 353
>sp|Q25605|EAAT_ONCVO Excitatory amino acid transporter (Sodium-dependent glutamate/
aspartate transporter)
Length = 492
Score = 247 bits (631), Expect = 2e-65
Identities = 123/167 (73%), Positives = 149/167 (89%), Gaps = 1/167 (0%)
Frame = -3
Query: 705 YIYQKESIKVF-HGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGATI 529
++ K++ VF G+ QAW+T LGTASSS TLPIT+ CLEEN GVD+RVTRFVLP+GATI
Sbjct: 289 FVTTKKNPYVFMRGLFQAWITGLGTASSSDTLPITYICLEENLGVDRRVTRFVLPVGATI 348
Query: 528 NMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVLTS 349
NMDGTALYEAVA+IFIAQING++L+FGQ+ T+S+TATLA+IGAASVPSAGLVTM+LVLT+
Sbjct: 349 NMDGTALYEAVAAIFIAQINGVHLSFGQVVTVSLTATLASIGAASVPSAGLVTMLLVLTA 408
Query: 348 VGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELEA 208
VGLP+ D++LI+AVDWLLDR+RTSINVLGDA GAGIV H K +L+A
Sbjct: 409 VGLPVKDVSLIVAVDWLLDRIRTSINVLGDAMGAGIVYHYSKADLDA 455
Score = 63.9 bits (154), Expect = 5e-10
Identities = 33/47 (70%), Positives = 37/47 (78%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNP 684
GKILEI DLA+TA L YMVTVL GLAIH I+L LIFF+ T+KNP
Sbjct: 250 GKILEIHDLADTARMLAMYMVTVLSGLAIHSLISLPLIFFVTTKKNP 296
>sp|P43006|EAA2_MOUSE Excitatory amino acid transporter 2 (Sodium-dependent
glutamate/aspartate transporter 2) (GLT-1)
Length = 572
Score = 247 bits (630), Expect = 3e-65
Identities = 118/169 (69%), Positives = 147/169 (86%)
Frame = -3
Query: 711 FLYIYQKESIKVFHGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGAT 532
+ + +K F G+ QAW+TALGTASS+ TLP+TF+CLE+N G+DKRVTRFVLP+GAT
Sbjct: 335 YFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEDNLGIDKRVTRFVLPVGAT 394
Query: 531 INMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVLT 352
INMDGTALYEAVA+IFIAQ+NG+ L GQI T+S+TATLA+IGAAS+PSAGLVTM+L+LT
Sbjct: 395 INMDGTALYEAVAAIFIAQMNGVILDGGQIVTVSLTATLASIGAASIPSAGLVTMLLILT 454
Query: 351 SVGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELEAI 205
+VGLP DI+L++AVDWLLDR+RTS+NV+GD+FGAGIV HL K EL+ I
Sbjct: 455 AVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTI 503
Score = 67.8 bits (164), Expect = 3e-11
Identities = 31/56 (55%), Positives = 40/56 (71%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNPLKYFTECYK 657
GKI+ IKDL A LG YM+TV++GL IHGGI L LI+F+ TRKNP +F ++
Sbjct: 297 GKIIAIKDLEVVARQLGMYMITVIVGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQ 352
>sp|P31596|EAA2_RAT Excitatory amino acid transporter 2 (Sodium-dependent
glutamate/aspartate transporter 2) (GLUT-R) (GLT-1)
Length = 573
Score = 247 bits (630), Expect = 3e-65
Identities = 118/169 (69%), Positives = 147/169 (86%)
Frame = -3
Query: 711 FLYIYQKESIKVFHGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGAT 532
+ + +K F G+ QAW+TALGTASS+ TLP+TF+CLE+N G+DKRVTRFVLP+GAT
Sbjct: 335 YFVVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEDNLGIDKRVTRFVLPVGAT 394
Query: 531 INMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVLT 352
INMDGTALYEAVA+IFIAQ+NG+ L GQI T+S+TATLA+IGAAS+PSAGLVTM+L+LT
Sbjct: 395 INMDGTALYEAVAAIFIAQMNGVILDGGQIVTVSLTATLASIGAASIPSAGLVTMLLILT 454
Query: 351 SVGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELEAI 205
+VGLP DI+L++AVDWLLDR+RTS+NV+GD+FGAGIV HL K EL+ I
Sbjct: 455 AVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTI 503
Score = 67.8 bits (164), Expect = 3e-11
Identities = 31/56 (55%), Positives = 40/56 (71%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNPLKYFTECYK 657
GKI+ IKDL A LG YM+TV++GL IHGGI L LI+F+ TRKNP +F ++
Sbjct: 297 GKIIAIKDLEVVARQLGMYMITVIVGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQ 352
>sp|P43003|EAA1_HUMAN Excitatory amino acid transporter 1 (Sodium-dependent
glutamate/aspartate transporter 1) (Glial glutamate
transporter) (GLAST-1)
Length = 542
Score = 244 bits (622), Expect = 3e-64
Identities = 129/184 (70%), Positives = 151/184 (82%), Gaps = 6/184 (3%)
Frame = -3
Query: 714 YFLYIYQKESIKVFHGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGA 535
YFL + +K G+LQA +TALGT+SSSATLPITFKCLEENNGVDKRVTRFVLP+GA
Sbjct: 337 YFL-VTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRVTRFVLPVGA 395
Query: 534 TINMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVL 355
TINMDGTALYEA+A+IFIAQ+N L FGQI TISITAT A+IGAA +P AGLVTM++VL
Sbjct: 396 TINMDGTALYEALAAIFIAQVNNFELNFGQIITISITATAASIGAAGIPQAGLVTMVIVL 455
Query: 354 TSVGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELE--AITTGN---- 193
TSVGLP +DITLI+AVDW LDR+RT+ NVLGD+ GAGIV+HL + EL+ + GN
Sbjct: 456 TSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIE 515
Query: 192 ENEM 181
ENEM
Sbjct: 516 ENEM 519
Score = 50.8 bits (120), Expect = 4e-06
Identities = 23/47 (48%), Positives = 32/47 (68%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNP 684
GKI+E++D+ L Y VTV++GL IH I L L++F+ TRKNP
Sbjct: 299 GKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNP 345
>sp|P46411|EAA1_BOVIN Excitatory amino acid transporter 1 (Sodium-dependent
glutamate/aspartate transporter 1) (GLAST1)
Length = 542
Score = 244 bits (622), Expect = 3e-64
Identities = 129/184 (70%), Positives = 151/184 (82%), Gaps = 6/184 (3%)
Frame = -3
Query: 714 YFLYIYQKESIKVFHGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGA 535
YFL + +K G+LQA +TALGT+SSSATLPITFKCLEENNGVDKRVTRFVLP+GA
Sbjct: 337 YFL-VTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRVTRFVLPVGA 395
Query: 534 TINMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVL 355
TINMDGTALYEA+A+IFIAQ+N L FGQI TISITAT A+IGAA +P AGLVTM++VL
Sbjct: 396 TINMDGTALYEALAAIFIAQVNNFELNFGQIITISITATAASIGAAGIPQAGLVTMVIVL 455
Query: 354 TSVGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELE--AITTGN---- 193
TSVGLP +DITLI+AVDW LDR+RT+ NVLGD+ GAGIV+HL + EL+ + GN
Sbjct: 456 TSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIE 515
Query: 192 ENEM 181
ENEM
Sbjct: 516 ENEM 519
Score = 50.8 bits (120), Expect = 4e-06
Identities = 23/47 (48%), Positives = 32/47 (68%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNP 684
GKI+E++D+ L Y VTV++GL IH I L L++F+ TRKNP
Sbjct: 299 GKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNP 345
>sp|P24942|EAA1_RAT Excitatory amino acid transporter 1 (Sodium-dependent
glutamate/aspartate transporter 1) (Glial glutamate
transporter) (GLAST) (GLAST-1)
Length = 543
Score = 243 bits (620), Expect = 5e-64
Identities = 128/184 (69%), Positives = 151/184 (82%), Gaps = 6/184 (3%)
Frame = -3
Query: 714 YFLYIYQKESIKVFHGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGA 535
YFL + +K G+LQA +TALGT+SSSATLPITFKCLEENNGVDKR+TRFVLP+GA
Sbjct: 337 YFL-VTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLPVGA 395
Query: 534 TINMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVL 355
TINMDGTALYEA+A+IFIAQ+N L FGQI TISITAT A+IGAA +P AGLVTM++VL
Sbjct: 396 TINMDGTALYEALAAIFIAQVNNFDLNFGQIITISITATAASIGAAGIPQAGLVTMVIVL 455
Query: 354 TSVGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELE--AITTGN---- 193
TSVGLP +DITLI+AVDW LDR+RT+ NVLGD+ GAGIV+HL + EL+ + GN
Sbjct: 456 TSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIE 515
Query: 192 ENEM 181
ENEM
Sbjct: 516 ENEM 519
Score = 52.0 bits (123), Expect = 2e-06
Identities = 24/47 (51%), Positives = 32/47 (68%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNP 684
GKILE++D+ L Y VTV++GL IH I L L++F+ TRKNP
Sbjct: 299 GKILEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNP 345
>sp|P56564|EAA1_MOUSE Excitatory amino acid transporter 1 (Sodium-dependent
glutamate/aspartate transporter) (High-affinity neuronal
glutamate transporter) (Glial high affinity glutamate
transporter) (GLAST)
Length = 543
Score = 243 bits (620), Expect = 5e-64
Identities = 128/184 (69%), Positives = 151/184 (82%), Gaps = 6/184 (3%)
Frame = -3
Query: 714 YFLYIYQKESIKVFHGMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGA 535
YFL + +K G+LQA +TALGT+SSSATLPITFKCLEENNGVDKR+TRFVLP+GA
Sbjct: 337 YFL-VTRKNPWVFIGGLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLPVGA 395
Query: 534 TINMDGTALYEAVASIFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVL 355
TINMDGTALYEA+A+IFIAQ+N L FGQI TISITAT A+IGAA +P AGLVTM++VL
Sbjct: 396 TINMDGTALYEALAAIFIAQVNNFDLNFGQIITISITATAASIGAAGIPQAGLVTMVIVL 455
Query: 354 TSVGLPINDITLILAVDWLLDRVRTSINVLGDAFGAGIVDHLCKKELE--AITTGN---- 193
TSVGLP +DITLI+AVDW LDR+RT+ NVLGD+ GAGIV+HL + EL+ + GN
Sbjct: 456 TSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIE 515
Query: 192 ENEM 181
ENEM
Sbjct: 516 ENEM 519
Score = 50.8 bits (120), Expect = 4e-06
Identities = 23/47 (48%), Positives = 32/47 (68%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNP 684
GKI+E++D+ L Y VTV++GL IH I L L++F+ TRKNP
Sbjct: 299 GKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNP 345
>sp|O57321|EAA1_AMBTI Excitatory amino acid transporter 1 (SEAAT1)
Length = 543
Score = 239 bits (611), Expect = 5e-63
Identities = 122/172 (70%), Positives = 146/172 (84%)
Frame = -3
Query: 669 GMLQAWVTALGTASSSATLPITFKCLEENNGVDKRVTRFVLPIGATINMDGTALYEAVAS 490
G+LQA +TALGT+SSSATLPITFKCLEENN VDKRVTRFVLP+GATINMDGTALYEA+A+
Sbjct: 352 GILQALITALGTSSSSATLPITFKCLEENNKVDKRVTRFVLPVGATINMDGTALYEALAA 411
Query: 489 IFIAQINGIYLTFGQIFTISITATLAAIGAASVPSAGLVTMILVLTSVGLPINDITLILA 310
IFIAQ+N L FGQI TISITAT A+IGAA +P AGLVTM++VLTSVGLP +DITLI+A
Sbjct: 412 IFIAQVNNYDLNFGQILTISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIA 471
Query: 309 VDWLLDRVRTSINVLGDAFGAGIVDHLCKKELEAITTGNENEMVTLDQNESK 154
VDW LDR+RT+ NVLGD+ GAGIV+HL + EL++ N ++ ++NE K
Sbjct: 472 VDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELQSGDAEMGNSVI--EENEMK 521
Score = 52.0 bits (123), Expect = 2e-06
Identities = 24/47 (51%), Positives = 31/47 (65%)
Frame = -1
Query: 824 GKILEIKDLANTASSLGSYMVTVLLGLAIHGGITLNLIFFIFTRKNP 684
GKI E++D+ LG Y VTV++GL IH I L L++F TRKNP
Sbjct: 300 GKIAEMEDMGVVGGQLGMYTVTVIIGLLIHAVIVLPLLYFAVTRKNP 346
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,271,961
Number of Sequences: 369166
Number of extensions: 1597334
Number of successful extensions: 4884
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4693
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4864
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7956112725
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)