Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_L07
(515 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cy... 146 2e-35
sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cyto... 144 1e-34
sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cy... 144 1e-34
sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cy... 143 2e-34
sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cy... 143 2e-34
sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mi... 142 4e-34
sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mi... 141 8e-34
sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP... 141 8e-34
sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cy... 139 4e-33
sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP... 129 4e-30
>sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 146 bits (369), Expect = 2e-35
Identities = 70/128 (54%), Positives = 87/128 (67%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL HWLS AH KLPRI+HVNWFRK + G FLWPG+GENSRVL+W+ R+ G
Sbjct: 488 YNFGKYLAHWLSMAHRPAAKLPRIFHVNWFRKDSQ-GKFLWPGYGENSRVLEWMFNRIQG 546
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
KA TA+G IP LNL GL+ + ELF++ EFW E EE++ YF+ Q+ DL
Sbjct: 547 KASAKSTAIGYIPADTALNLKGLE--DINLTELFNISKEFWEKEVEEIKQYFEGQVNADL 604
Query: 407 PEEIKVEL 430
P EI+ EL
Sbjct: 605 PYEIEREL 612
>sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 144 bits (363), Expect = 1e-34
Identities = 67/134 (50%), Positives = 90/134 (67%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL HWLS AH KLP+I+HVNWFRK K +G FLWPGFGENSRVL+W+ R+ G
Sbjct: 488 YNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDK-NGKFLWPGFGENSRVLEWMFGRIEG 546
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
+ T +G +P+ + LNL GL + +ELF + EFW E EE++ Y ++Q+ DL
Sbjct: 547 EDSAKLTPIGYVPKEDALNLKGL--GDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADL 604
Query: 407 PEEIKVELQNFRHR 448
P EI+ EL+ + R
Sbjct: 605 PYEIERELRALKQR 618
>sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 144 bits (363), Expect = 1e-34
Identities = 69/134 (51%), Positives = 88/134 (65%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL HWLS AH KLP+I+HVNWFRK K DG FLWPGFGENSRVL+W+ R+ G
Sbjct: 488 YNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDK-DGKFLWPGFGENSRVLEWMFGRIEG 546
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
+ T +G IP+ LNL GL +ELF + EFW E EE++ Y ++Q+ DL
Sbjct: 547 EDSAKLTPIGYIPKENALNLKGL--GGVNVEELFGISKEFWEKEVEEIDRYLEDQVNTDL 604
Query: 407 PEEIKVELQNFRHR 448
P EI+ EL+ + R
Sbjct: 605 PYEIERELRALKQR 618
>sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 143 bits (361), Expect = 2e-34
Identities = 70/134 (52%), Positives = 88/134 (65%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL HWLS A KLP+I+HVNWFRK KE G FLWPGFGENSRVL+W+ R+ G
Sbjct: 488 YNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKE-GKFLWPGFGENSRVLEWMFNRIDG 546
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
KA T +G IP+ + LNL GL ELFS+ EFW E E++E Y ++Q+ DL
Sbjct: 547 KASTKLTPIGYIPKEDALNLKGL--GHINMMELFSISKEFWEKEVEDIEKYLEDQVNADL 604
Query: 407 PEEIKVELQNFRHR 448
P EI+ E+ + R
Sbjct: 605 PCEIEREILALKQR 618
>sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 143 bits (361), Expect = 2e-34
Identities = 70/134 (52%), Positives = 88/134 (65%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL HWLS A KLP+I+HVNWFRK KE G FLWPGFGENSRVL+W+ R+ G
Sbjct: 488 YNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKE-GKFLWPGFGENSRVLEWMFNRIDG 546
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
KA T +G IP+ + LNL GL ELFS+ EFW E E++E Y ++Q+ DL
Sbjct: 547 KAGAKLTPIGYIPKEDALNLKGL--GHINVMELFSISKEFWEKEVEDIEKYLEDQVNADL 604
Query: 407 PEEIKVELQNFRHR 448
P EI+ E+ + R
Sbjct: 605 PCEIEREILALKQR 618
>sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 142 bits (359), Expect = 4e-34
Identities = 64/134 (47%), Positives = 91/134 (67%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL+HWLS +LPRI+HVNWFR+ E G FLWPGFGEN+RVLDWI +R+ G
Sbjct: 506 YNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRD-EAGHFLWPGFGENARVLDWICRRLEG 564
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
+ +T +G++P+ L+LSGL+ + +LFSLP +FW E ++ +Y EQ+ +DL
Sbjct: 565 EDSARETPIGLVPKEGALDLSGLRAIDT--TQLFSLPKDFWEQEVRDIRSYLTEQVNQDL 622
Query: 407 PEEIKVELQNFRHR 448
P+E+ EL+ R
Sbjct: 623 PKEVLAELEALERR 636
>sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 141 bits (356), Expect = 8e-34
Identities = 63/134 (47%), Positives = 90/134 (67%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL+HWLS + +LPRI+HVNWFR+ E G FLWPGFGEN+RVLDWI +R+ G
Sbjct: 506 YNFGRYLEHWLSMEGQKGARLPRIFHVNWFRRD-EAGRFLWPGFGENARVLDWICRRLEG 564
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
+ +T +G++P+ L+LSGL + +LFS+P +FW E ++ Y EQ+ +DL
Sbjct: 565 EDSAQETPIGLVPKEGALDLSGLSAVDT--SQLFSIPKDFWEQEVRDIRGYLTEQVNQDL 622
Query: 407 PEEIKVELQNFRHR 448
P+E+ EL+ R
Sbjct: 623 PKEVLAELEALEGR 636
>sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 647
Score = 141 bits (356), Expect = 8e-34
Identities = 67/134 (50%), Positives = 92/134 (68%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFGDY+ HWLS E ++P+I+HVNWFRKS E G F+WPG+GENSRVL+WI +RV G
Sbjct: 514 YNFGDYVAHWLSM--EKRGQVPKIFHVNWFRKSAE-GKFMWPGYGENSRVLEWILRRVNG 570
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
++ + +A+G IP LNL G+K + KE+FSLP EFW+ E +++ YF+ Q+G DL
Sbjct: 571 ESCYVDSAIGHIPAEGALNLDGMK-DKVDVKEIFSLPKEFWSQEVKDIRTYFESQVGADL 629
Query: 407 PEEIKVELQNFRHR 448
P I +L R
Sbjct: 630 PASIYQQLDELSSR 643
>sp|Q8HYZ4|PPCKC_BOVIN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 139 bits (350), Expect = 4e-33
Identities = 68/134 (50%), Positives = 86/134 (64%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL HWLS A KLP+I+HVNWFRK K G FLWPGFGENSRVL+W+ RV G
Sbjct: 488 YNFGQYLAHWLSMAQRPAAKLPKIFHVNWFRKDKA-GRFLWPGFGENSRVLEWMFNRVVG 546
Query: 227 KAMGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKDL 406
+ T +G IP + L+L GL + KELF + EFW E EE++ Y +EQ+ DL
Sbjct: 547 EGGASVTPIGYIPDEDALDLRGL--GDVDVKELFHISKEFWEEEVEEIQKYLEEQVNVDL 604
Query: 407 PEEIKVELQNFRHR 448
P EIK ++ + R
Sbjct: 605 PPEIKNQVLALKQR 618
>sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 619
Score = 129 bits (324), Expect = 4e-30
Identities = 57/129 (44%), Positives = 91/129 (70%), Gaps = 1/129 (0%)
Frame = +2
Query: 47 HNFGDYLKHWLSFAHESHYKLPRIYHVNWFRKSKEDGSFLWPGFGENSRVLDWIAKRVAG 226
+NFG YL+HW+ + K+P+IYHVNWFR+ + FLWPG+G+N RV+DWI +R+ G
Sbjct: 484 YNFGHYLQHWIDLNKDGR-KVPKIYHVNWFRRDANN-KFLWPGYGQNIRVIDWIVRRLDG 541
Query: 227 KA-MGLKTAVGIIPRFEELNLSGLKITEKQYKELFSLPSEFWNHEFEELEAYFKEQIGKD 403
+ +G+ T +GI+P+ +N SGL + Q+ EL S+P E+W ++ +E+ + +EQ+G D
Sbjct: 542 EPDIGVDTPIGIVPKKGAINASGLP--DIQWDELMSVPKEYWTNDAKEIRKFLEEQVGPD 599
Query: 404 LPEEIKVEL 430
LP+EI+ E+
Sbjct: 600 LPKEIRAEM 608
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,703,581
Number of Sequences: 369166
Number of extensions: 1044777
Number of successful extensions: 3315
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3232
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3354254700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)